| Genetic variation of Porphyra yezoensis by using AFLP1 |
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| 作者姓名： | Rui Yang Biqian Liu Qijun Luo Yajun Wang Jiamei Bao |
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| 作者单位： | Rui Yang，Biqian Liu，Qijun Luo，Yajun Wang，Jiamei Bao Marine Biotechnology Institution，Ningbo University，Ningbo 315211，China.E-mail:yangrui @ nbu.edu.cn |
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| 基金项目： | National Natural Science Foundation of China under contract No. 49976030，Key Ph D Foundation of Ningbo City under contract No. 02J20110-15 and No. 01N40100-59 |
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| 摘 要： | Genetic variation of 11 lines of Porphyra yezoensis from the coastline of Kagoshima ofJapan, Qingdao, Nantong, Putuo and Nanji Islands of China were studied by using amplified fragment length polymorphism (AFLP).778 bands were obtained with AFLP analysis of 16 primer combinations, among which 15 were unique, about 98.07% were polymorphic. The AFLP data showed that the closest genetic distance was 0.180 between two Kagoshima samples, and the farthest one was 0.397 between Kagoshima No.1 and Nantong No.9 line. The genetic distance showed that the variation was within the inner species scope. Neighbor-joining cluster and UPGMA cluster indicated that samples from Kagoshima and Qingdao were with high similarity and either with the samples of Nantong, Putuo and Nanji Islands. P. yezoensis in China shared high genetic diversity, and the genetic distance showed positive correlation with the geographic distance.
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| 关 键 词： | Porphyra yezoensis AFLP genetic variation |
| 收稿时间： | 6/8/2002 12:00:00 AM |
| 修稿时间： | 2002/12/30 0:00:00 |
Genetic variation of Porphyra yezoensis by using AFLP1 |
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| Rui Yang,Biqian Liu,Qijun Luo,Yajun Wang,Jiamei Bao.Genetic variation of Porphyra yezoensis by using AFLP1[J].Acta Oceanologica Sinica,2003,22(3):453-457. |
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| Authors: | Rui Yang Biqian Liu Qijun Luo Yajun Wang and Jiamei Bao |
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| Institution: | Marine Biotechnology Institution, Ningbo University, Ningbo 315211, China |
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| Abstract: | Genetic variation of 11 lines of Porphyra yezoensis from the coastline of Kagoshima ofJapan, Qingdao, Nantong, Putuo and Nanji Islands of China were studied by using amplified fragmentlength polymorphism (AFLP). 778 bands were obtained with AFLP analysis of 16 primer combina-tions, among which 15 were unique, about 98.07% were polymorphic. The AFLP data showed thatthe closest genetic distance was 0.180 between two Kagoshima samples, and the farthest one was 0.397between Kagoshima No. 1 and Nantong No. 9 line. The genetic distance showed that the variation waswithin the inner species scope. Neighbor-joining cluster and UPGMA cluster indicated that samples fromKagoshima and Qingdao were with high similarity and either with the samples of Nantong, Putuo andNanji Islands. P. yezoensis in China shared high genetic diversity, and the genetic distance showed posi-tive correlation with the geographic distance. |
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| Keywords: | Porphyra yezoensis AFLP genetic variation |
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