| 南大西洋深海沉积物中可培养放线菌的多样性 |
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| 引用本文： | 张玉便,张改云.南大西洋深海沉积物中可培养放线菌的多样性[J].台湾海峡,2014(4):508-515. |
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| 作者姓名： | 张玉便 张改云 |
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| 作者单位： | 国家海洋局第三海洋研究所 国家海洋局海洋生物遗传资源重点实验室,福建 厦门,361005 |
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| 基金项目： | 国际海域资源调查与开发“十二五”重大资助项目 |
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| 摘 要： | 探究了南大西洋深海沉积物中可培养放线菌的多样性,筛选药源活性次级代谢产物产生菌,为后续资源开发奠定基础.采用3种预处理方法及8种选择性培养基对南大西洋3个深海沉积物样品中的放线菌菌株进行选择性分离鉴定;利用兼并引物扩增法,选取代表菌株进行聚酮合酶（PKSⅠ、PKSⅡ）基因和非核糖体多肽合成酶（NRPS）基因的检测;以4株细菌为指示菌检测代表菌株的抑菌活性.共分离得到132株放线菌纯菌株,分布于放线菌亚纲的6个目、13个科、19个属中,其中有5个属为较新或较稀有种属,有2株为潜在新种.34株化合物合成基因检测菌中PKSⅠ基因、PKSⅡ基因呈阳性的比率均为17.64%,NRPS基因呈阳性的则为52.94%.抗枯草芽孢杆菌（Bacillus subtilis）和抗副溶血性弧菌（Vibrio parahaemolyticus）的菌株分别有47.06%和7.82%.南大西洋深海沉积物中放线菌资源丰富,存在较多潜在新分类单元,筛选到的活性菌株可用于后续药源活性次级代谢产物的分离.
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| 关 键 词： | 海洋生物学 放线菌 深海 多样性 功能基因 |
Diversity of cultivable actinomycetes in deepsea sediments of the South Atlantic Ocean |
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| ZHANG Yu-bian,ZHANG Gai-yun.Diversity of cultivable actinomycetes in deepsea sediments of the South Atlantic Ocean[J].Journal of Oceanography In Taiwan Strait,2014(4):508-515. |
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| Authors: | ZHANG Yu-bian ZHANG Gai-yun |
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| Institution: | (Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, SOA, Xiamen 361005, China) |
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| Abstract: | To investigate the diversity of cultivable actinomycetes in South Atlantic Ocean and screen actinomycetes with a pharmaceutical potential for producing biologically active secondary metabolites.Three different treatment approaches and 8 selective media were used to isolate actinomycetes from 3 sediment samples.Used degenerate oligonucleotide primers,representative strains were screened for genes encoding type I and type II polyketide synthases and nonribosomal peptide synthases by polymerase chain reaction.Antimicrobial activity was determined by observing bacterial growth inhibition.A total of 132 actinomycetes were isolated from 3 sediment samples collected from South Atlantic Ocean.The isolates can be assigned to 6 orders,13 families and 19 genera,in which strains YB250 and YB268 maybe 2 putative novel species.Nonribosomal peptide synthetase sequences were detected in most of the isolates（52.94%） whereas both PKS-I and PKS-II sequences were detected in only six out of the 34strains（17.64%）.Out of the 34 tested strains,16 strains（47.06%） exhibited inhibition activity against Bacillus subtilis and 3 strains（7.82%） against Vibrio parahaemolyticus.The deep-sea environments of South Atlantic Ocean harbour highly diverse actinobacterial communities,many of which appear to be novel candidate species.The actinomycetes having antimicrobial activity could be a promising source for antimicrobial bioactive agents. |
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| Keywords: | marine biology actinomycetes deepsea diversity functional gene |
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