| 10种蟹类18S rDNA和COⅠ基因序列分析及其分子系统发育研究 |
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| 引用本文： | 邢炳鹏,林荣澄,徐宪忠,牛文涛.10种蟹类18S rDNA和COⅠ基因序列分析及其分子系统发育研究[J].海洋学研究,2012(2):86-92. |
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| 作者姓名： | 邢炳鹏 林荣澄 徐宪忠 牛文涛 |
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| 作者单位： | 国家海洋局第三海洋研究所 |
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| 基金项目： | 中国大洋协会国际海底区域研究开发“十一五”资助项目(DYXM-115-02-4-05) |
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| 摘 要： | 应用分子系统发育学的方法,以蟹类18S rDNA和线粒体细胞色素C氧化酶亚单位(COI)基因序列片段为分子标记,结合形态学特征对10种蟹类的分类地位进行探讨。实验共获得10条18S rDNA序列,长度为1780～1787 bp,其中A、T、G、C平均含量分别为23.72%,24.58%,24.52%和27,17%;通过序列对比,发现18S rDNA序列相对保守,只含有1个从88 bp到130 bp约5()bp的高可变区;物种澡碱基距离比较小,从0.001到0.017。18S rDNA系统发育树为方蟹总科、沙蟹总科和梭子蟹总科起源于同一海洋蟹类祖先提供了分子生物学证据,并且支持将厚蟹属从相手蟹科移至弓蟹科。获得的5条COI基因序列,长度均为709 bp,A、T、G、C平均含量分别为26.88%,37.62%,17.50%和18.00%;COI基因片段变异性高,物种澡碱基距离从0.016到0.138。COⅠ基因系统发育树真实地反映了蟳属各物种之间的进化关系,和传统分类非常吻合,为形态特征相似的蟳类鉴定提供了一种快速准确的方法。
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| 关 键 词： | 蟹类 18S rDNA COⅠ 分子系统发育 |
18S rDNA and CO I gene sequence analysis of 10 crabs and study of their molecular phylogeny |
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| XING Bing-peng,LIN Rong-cheng,XU Xian-zhong,NIU Wen-tao.18S rDNA and CO I gene sequence analysis of 10 crabs and study of their molecular phylogeny[J].Journal of Marine Sciences,2012(2):86-92. |
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| Authors: | XING Bing-peng LIN Rong-cheng XU Xian-zhong NIU Wen-tao |
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| Institution: | (Third Institute of Oceanography,SOA,Xianmen 361005,China) |
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| Abstract: | Using molecular phylogenetic methodology with gene sequence fragments of 18S rDNA and subunitⅠof C enzyme oxide of mitochondrial dell pigment(COⅠ) as the molecular mark and combined with morphological character,evolutionary history of selected brachyuran crabs were discussed.Totally 10 18S rDNA sequnces and 5 COⅠgene fragments were obtained by Polymerase Chain Reaction(PCR),and summited to the Genebank.The length of 18S rDNA sequences from 10 crabs,ranges from 1 780 to 1 787 bp, in which the shortest one is Hemigrapsus sinensis,the longest one is Parthenope validus.In the 18S rDNA sequnces,the average content of base A,T,G and C is 23.72%,24.58%,24.52%,and 27.17%separately. Sequence alignment shows that 18S rDNA sequences are relatively conservative,containing only one hypervariable region of about 50 bp between 88 bp and 130 bp.The base distance of the species is very small, from 0.001 to 0.017.The phylogenetic trees of 18S rDNA give molecular biological proof for the same origin of Grapsioidea,Ocypodioidea and Portunoidea,and also support moving the Helice crab from Sesarminae to Varuninae.The length of mitochondrial DNA sequence from 5 charybdis crabs is 709 bp,and the average content of the base A,T,G and C is 26.88%,37.62%,17.50%and 18.00%separately.The fragment of COⅠgene has high species variability,more suitable for the study of the interspecific evolutionary history. The base distance of the charybdis is long,from 0.016 to 0.138.The phylogenetic trees of COⅠgene true reflect the evolutionary relations of different charybdis species and are concordant with traditional taxology. This provides a molecular method for the identification of charybdis species of similar morphological characters. |
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| Keywords: | crab 18S rDNA COⅠ phylogenesis |
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