| 一株产褐藻酸多糖的海洋假单胞细菌Pseudomonas sp.QDA的筛选和鉴定 |
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| 引用本文： | 韩文君,路新枝,肖琳,于文功.一株产褐藻酸多糖的海洋假单胞细菌Pseudomonas sp.QDA的筛选和鉴定[J].中国海洋大学学报(自然科学版),2004,34(1):60-64. |
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| 作者姓名： | 韩文君 路新枝 肖琳 于文功 |
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| 作者单位： | 中国海洋大学海洋药物与食品研究所,山东,青岛,266003 |
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| 基金项目： | 教育部优秀青年教师资助计划项目 ( 2 0 0 0 2 - 350 )资助 |
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| 摘 要： | 根据已获褐藻酸多糖生物合成基因簇中褐藻胶裂解酶基因(αlgL)的序列设计特异性引物，利用PCR技术从海洋微生物中筛选到1株能够分泌胞外多糖的细菌。采用形态学观察和16S rDNA序列分析鉴定该菌株，结果为假单胞属细菌，命名为Pseudomonas sp.QDA；系统发育树显示该菌株与P.putida亲缘关系最近。菌株产生的胞外多糖可被褐藻胶裂解酶(AlgL)降解，并在紫外234nm处检测到特征性吸收，初步证明含有褐藻酸多糖。
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| 关 键 词： | 褐藻胶裂解酶基因 PCR 海洋假单胞细菌 16S rDNA |
| 文章编号： | 1672-1574(2004)01-060-05 |
| 修稿时间： | 2003年3月5日 |
Screening of an Alginate-Producing Marine Bacterium Pseudomonas sp.Strain QDA and its Characterization |
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| HAN Wen-jun,LU Xin-zhi,XIAO Lin,YU Wen-gong.Screening of an Alginate-Producing Marine Bacterium Pseudomonas sp.Strain QDA and its Characterization[J].Periodical of Ocean University of China,2004,34(1):60-64. |
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| Authors: | HAN Wen-jun LU Xin-zhi XIAO Lin YU Wen-gong |
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| Abstract: | Based on the DNA sequence of a novel alginate lyase gene (algL) that was cloned from a marine environmental DNA library, specific primer pairs were designed and used in PCR screening of an alginate-producing marine bacterium. With the method of phenotype observation and sequence analysis of the 16S rDNA, a marine bacterium was identified as some Pseudomonas that was most closely related to P.putida in the phylogenetic tree. The deacetylated exocellular polysaccharides could be degraded by a defined alginate lyase (AlgL) and the scanning graph of the digested mixture showed a characteristic absorption at 234nm, which suggested the existence of alginate. |
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| Keywords: | alginate lyase gene PCR marine Pseudomonas 16S rDNA |
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