| 利用AFLP技术研究条斑紫菜的遗传变异 |
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| 引用本文： | 杨锐,刘必谦,骆其君,王亚军,包佳美.利用AFLP技术研究条斑紫菜的遗传变异[J].海洋学报,2005,27(3):159-162. |
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| 作者姓名： | 杨锐 刘必谦 骆其君 王亚军 包佳美 |
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| 作者单位： | 1.中国科学院, 海洋研究所, 实验海洋生物学重点实验室, 山东, 青岛, 266071;宁波大学, 海洋生物工程重点实验室, 浙江, 宁波, 315211 |
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| 基金项目： | 浙江省重点项目(2004C22045)，浙江省自然科学基金(M403015)，宁波市重点博士基金资助项目(02J2011015). |
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| 摘 要： | 利用AFLP技术对11个条斑紫菜品系进行了研究.由16对引物共扩增出778条谱带,其中仅15条为共有谱带,多态位点的比例高达98.07%.日本鹿儿岛,我国的青岛、江苏、浙江等不同地点的野生或栽培条斑紫菜品系之间最近的遗传距离为0.180(两个日本样本之间),最远为0.397(日本样本与江苏样本之间),属于物种内种群间的遗传变异.聚类结果显示日本的样本与青岛的野生样本间的遗传相似系数较高而聚合在一起;浙江野生条斑紫菜与江苏的样本遗传距离相近,聚合在一起.结果表明,我国条斑紫菜的遗传多样性程度较高而且各品系间的遗传距离与其地理间距呈正相关关系.
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| 关 键 词： | 条斑紫菜 AFLP 遗传变异 |
| 文章编号： | 0253-4193(2005)03-0159-04 |
| 收稿时间： | 7/8/2003 12:00:00 AM |
| 修稿时间： | 2003/12/30 0:00:00 |
Genetic variation of Porphyra yezoensis by using AFLP |
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| YANG Rui,LIU Bi-qian,LUO Qi-jun,WANG Ya-jun and BAO Jia-mei.Genetic variation of Porphyra yezoensis by using AFLP[J].Acta Oceanologica Sinica (in Chinese),2005,27(3):159-162. |
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| Authors: | YANG Rui LIU Bi-qian LUO Qi-jun WANG Ya-jun and BAO Jia-mei |
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| Institution: | 1.Key Laboratory of Experimental Marine Biology, Institite of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;Key Laboratory of Marine Biotechnology, Ningbo University, Ningbo 315211, China2.Key Laboratory of Marine Biotechnology, Ningbo University, Ningbo 315211, China |
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| Abstract: | Genetic variation of 11 lines of Porphyra yezoensis from the coast seas of Kagoshima (Japan), Qingdao, Nantong, Putuo and Naiji islands were studied by using amplified fragment length polymorphism(AFLP). 778 bands are obtained with AFLP analysis of 16 primer combinations, among which 15 are unique, about 98.07% are polymorphic. The AFLP data show that the closest genetic distance are 0.180 between two Kagoshima samples, and the furthest one 0.397 is between Kagoshima No.1 line and Nantong No.9 line. The genetic distance shows that the variation is within the inner species scope. Neighbor-joining cluster and UPGMA cluster indicate that samples from Kagoshima and Qingdao were with high similarity and either with the samples of Nantong, Putuo and Nanji islands. Porphyra yezoensis in China shares high genetic diversity, and the genetic distance shows positive correlation with the geographic distance. |
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| Keywords: | Porphyra yezoensis AFLP genetic variation |
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