| 大泷六线鱼(Hexagrammos otakii)荧光AFLP分析体系的建立及优化 |
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| 引用本文： | 仝颜丽,李海东,王玲,王波,郑明刚.大泷六线鱼(Hexagrammos otakii)荧光AFLP分析体系的建立及优化[J].海洋通报,2011(6):668-673. |
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| 作者姓名： | 仝颜丽 李海东 王玲 王波 郑明刚 |
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| 作者单位： | 日照职业技术学院，山东 日照 276826;日照职业技术学院，山东 日照 276826;青岛大学，山东 青岛 266061;国家海洋局第一海洋研究所 海洋生态中心，山东 青岛 266061;国家海洋局第一海洋研究所 海洋生态中心，山东 青岛 266061 |
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| 基金项目： | 国家海洋公益性行业科研专项（201009）；国家农业科技成果转化资金项目（2009-511-208） |
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| 摘 要： | 以大泷六线鱼为材料,通过对DNA提取质量和浓度、EcoR Ⅰ/MseⅠ酶切反应时间、Mg2+浓度、dNTPs浓度、引物浓度以及选择性扩增中预扩增产物稀释倍数等关键因素进行研究,建立并优化了大泷六线鱼荧光AFLP分子标记反应体系.用优化的反应体系,筛选到10对适合大泷六线鱼的引物,为今后利用AFLP标记技术进行大泷六线鱼...
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| 关 键 词： | 大泷六线鱼 AFLP 分子标记 反应体系 |
| 收稿时间： | 2010/8/19 0:00:00 |
| 修稿时间： | 3/7/2011 12:00:00 AM |
Establishment and optimization of fluorescence-based AFLP markers for Hexagrammos otakiis |
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| TONG Yan-li,LI Hai-dong,WANG Ling,WANG Bo and ZHENG Ming-gang.Establishment and optimization of fluorescence-based AFLP markers for Hexagrammos otakiis[J].Marine Science Bulletin,2011(6):668-673. |
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| Authors: | TONG Yan-li LI Hai-dong WANG Ling WANG Bo and ZHENG Ming-gang |
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| Institution: | Rizhao Polytechnic, Rizhao 276826, China;Rizhao Polytechnic, Rizhao 276826, China;Qingdao University, Qingdao 266061, China;The First Institute of Oceanography, Qingdao 266061, China;The First Institute of Oceanography, Qingdao 266061, China |
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| Abstract: | In this study, we optimized the main factors influencing the results of AFLP, including the quality and concentration of the extracted DNA, reaction time of enzymes EcoRI/MseI digestion, and the concentration of Mg2+, dNTPs, selective primer and the dilute multiple of pre-amplification production were also optimized in selective amplification reaction system. Ten pairs of primers were identified from eighty-one pairs of primers by optimizing the AFLP reaction system. The result established a strong foundation for the AFLP analysis of H. otakii which provided a standardized program for studying genetic diversity of H. Otakii. |
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| Keywords: | Hexagrammos otaki fluorescence-based AFLP markers reaction system |
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