| 基于线粒体COI 基因的毛蚶群体遗传多样性 |
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| 引用本文： | 田吉腾,侯 丫,刘志鸿,杨爱国,吴 彪,周丽青,董春光.基于线粒体COI 基因的毛蚶群体遗传多样性[J].海洋科学,2016,40(1):1-9. |
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| 作者姓名： | 田吉腾 侯 丫 刘志鸿 杨爱国 吴 彪 周丽青 董春光 |
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| 作者单位： | 农业部海洋渔业可持续发展重点实验室, 中国水产科学研究院 黄海水产研究所,农业部海洋渔业可持续发展重点实验室, 中国水产科学研究院 黄海水产研究所；上海海洋大学 水产与生命学院,农业部海洋渔业可持续发展重点实验室, 中国水产科学研究院 黄海水产研究所,农业部海洋渔业可持续发展重点实验室, 中国水产科学研究院 黄海水产研究所,农业部海洋渔业可持续发展重点实验室, 中国水产科学研究院 黄海水产研究所,农业部海洋渔业可持续发展重点实验室, 中国水产科学研究院 黄海水产研究所,农业部海洋渔业可持续发展重点实验室, 中国水产科学研究院 黄海水产研究所；上海海洋大学 水产与生命学院 |
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| 基金项目： | 国家科技部基础工作专项 (2013FY110700) ; 黄海水产研究所级基本科研业务费资助(20603022013032) |
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| 摘 要： | 采用PCR技术,扩增了大连、乳山、烟台、舟山4个毛蚶(Scapharca subcrenata)地理群体共38个个体的线粒体COI基因部分序列,并分析了4个毛蚶群体的遗传多样性和系统发育关系。研究结果显示:38个毛蚶COI部分序列经处理得到长度均为625bp的基因片段,共分为30种单倍型;基于COI部分序列的分析结果,毛蚶4个地理群体总的变异位点为301个,多样性指数Pi为0.15048,平均核苷酸差异数为92.242,单倍型多样性指数S为241。聚类分析显示毛蚶大连群体、乳山群体和烟台群体具有高度的遗传多样性,3个群体交叉聚在一起,没有明显的群体分化;舟山群体单独聚为一支,与其他3个群体分化明显。研究表明,线粒体COI基因不能单独做为毛蚶大连、乳山和烟台群体的遗传标记,但可以作为毛蚶舟山群体的有效群体遗传标记,为线粒体COI基因在群体遗传学的应用提供了基础资料。
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| 关 键 词： | 毛蚶(Scapharca subcrenata) COI 基因 群体 遗传多样性 |
| 收稿时间： | 5/9/2015 12:00:00 AM |
| 修稿时间： | 2015/9/10 0:00:00 |
Genetic diversity of different populations of Scapharca subcrenata based on mitochondrial COI gene |
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| TIAN Ji-teng,HOU Y,LIU Zhi-hong,YANG Ai-guo,WU Biao,ZHOU Li-qing and DONG Chun-guang.Genetic diversity of different populations of Scapharca subcrenata based on mitochondrial COI gene[J].Marine Sciences,2016,40(1):1-9. |
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| Authors: | TIAN Ji-teng HOU Y LIU Zhi-hong YANG Ai-guo WU Biao ZHOU Li-qing and DONG Chun-guang |
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| Institution: | Key Laboratory of sustainable development of marine fisheries, Ministry of agriculture, the Yellow Sea Institute of fisheries, Chinese Academy of Fishery Sciences,Key Laboratory of sustainable development of marine fisheries, Ministry of agriculture, the Yellow Sea Institute of fisheries, Chinese Academy of Fishery Sciences; College of Fisheries and Life Sciences, Shanghai Ocean University,Key Laboratory of sustainable development of marine fisheries, Ministry of agriculture, the Yellow Sea Institute of fisheries, Chinese Academy of Fishery Sciences,Key Laboratory of sustainable development of marine fisheries, Ministry of agriculture, the Yellow Sea Institute of fisheries, Chinese Academy of Fishery Sciences,Key Laboratory of sustainable development of marine fisheries, Ministry of agriculture, the Yellow Sea Institute of fisheries, Chinese Academy of Fishery Sciences,Key Laboratory of sustainable development of marine fisheries, Ministry of agriculture, the Yellow Sea Institute of fisheries, Chinese Academy of Fishery Sciences and Key Laboratory of sustainable development of marine fisheries, Ministry of agriculture, the Yellow Sea Institute of fisheries, Chinese Academy of Fishery Sciences; College of Fisheries and Life Sciences, Shanghai Ocean University |
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| Abstract: | General PCR technology was used for the amplification of mitochondrial COI gene partial sequences of four groups of Scapharca subcrenata (Dalian, Rushan, Yantai, and Zhoushan). A total of 38 sequences from different populations were determined and analyzed. The results are as follows: (a) After excluding both sides of the inaccurate sequences, 38 COI gene partial sequences of S. subcrenata, 625 bp each, were eventually identified, among which 30 haploid types were detected. (b) Based on partial COI gene sequence analysis of the four groups of S. subcrenata, the total variation loci, haploid type diversity index S, nucleotide diversity index Pi, and average nucleotide difference index were 301, 241, 0.15048, and 92.242, respectively. (c) Cluster analysis showed that ark shells from three groups, Rushan, Dalian, and Yantai, had a high degree of genetic diversity, and they clustered across, without the obvious characteristics of the groups. However, ark shells from the Zhoushan group gathered themselves together, apart from the other three groups obviously. The genetic diversity index of Zhoushan group was much lower than that of other groups. (d) Mitochondrial COI gene could not separate the ark shells from Rushan, Dalian, and Yantai but can be a marker for Zhoushan populations of S. subcrenata, which provides more basic data for the application of mitochondrial COI gene in population genetics. |
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| Keywords: | Scapharca subcrenata COI gene population diversity |
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