| RP-HPLC法同时测定翅碱蓬中四种黄酮化合物的含量 |
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| 引用本文： | 郭丽霞,宁寰宇,曹苇,李慧,孔亮,谭成玉.RP-HPLC法同时测定翅碱蓬中四种黄酮化合物的含量[J].海洋科学,2020,44(4):111-115. |
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| 作者姓名： | 郭丽霞 宁寰宇 曹苇 李慧 孔亮 谭成玉 |
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| 作者单位： | 大连海洋大学海洋科技与环境学院,辽宁大连116023;大连海洋大学海洋科技与环境学院,辽宁大连116023;大连海洋大学海洋科技与环境学院,辽宁大连116023;大连海洋大学海洋科技与环境学院,辽宁大连116023;大连海洋大学海洋科技与环境学院,辽宁大连116023;大连海洋大学海洋科技与环境学院,辽宁大连116023 |
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| 基金项目： | 辽宁省自然科学基金重点项目（20170540102），盘锦红海滩湿地退化与生态修复项目（PHL-XZ-2017013002） |
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| 摘 要： | 建立RP-HPLC(反相高效液相色谱法)同时测定翅碱蓬中四种黄酮化合物-芸香苷、槲皮素3-β-D-葡萄糖苷、水仙苷和槲皮素的含量。采用Kromasil C18色谱柱(4.6 mm×250 mm,5μm),检测波长为361nm,柱温为30℃,流动相为甲醇-0.4%冰乙酸(55︰45),流速1.0mL/min,四种黄酮类化合物得到很好的分离。芸香苷、槲皮素3-β-D-葡萄糖苷、水仙苷和槲皮素浓度均为0.001~0.01 mg/mL范围内呈现良好线性关系,相关系数R2在0.9990~0.9996,加标回收率(n=3)范围为82.39%~99.03%。该方法简单、快速、高效,可以用于翅碱蓬中四种黄酮化合物的含量分析。
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| 关 键 词： | 翅碱蓬 反相高效液相色谱 芸香苷 槲皮素3-β-D-葡萄糖苷 水仙苷 槲皮素 |
| 收稿时间： | 2019/7/16 0:00:00 |
| 修稿时间： | 2019/10/6 0:00:00 |
Simultaneous determination of four flavonoids in Suaeda Heteroptera by RP-HPLC |
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| GUO Li-xi,NING Huan-yu,CAO Wei,LI Hui,KONG Liang and TAN Cheng-yu.Simultaneous determination of four flavonoids in Suaeda Heteroptera by RP-HPLC[J].Marine Sciences,2020,44(4):111-115. |
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| Authors: | GUO Li-xi NING Huan-yu CAO Wei LI Hui KONG Liang and TAN Cheng-yu |
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| Institution: | College of Marine Science and Environment, Dalian Ocean University, Dalian 116023, China,College of Marine Science and Environment, Dalian Ocean University, Dalian 116023, China,College of Marine Science and Environment, Dalian Ocean University, Dalian 116023, China,College of Marine Science and Environment, Dalian Ocean University, Dalian 116023, China,College of Marine Science and Environment, Dalian Ocean University, Dalian 116023, China and College of Marine Science and Environment, Dalian Ocean University, Dalian 116023, China |
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| Abstract: | In this study,we established a reversed-phase high-performance liquid chromatography(RP-HPLC)method for the simultaneous determination of four flavonoids-rutin,quercetin 3-β-D-glucoside,narcissoside,and quercetin-in Suaeda Heteroptera.The separation was achieved using a Kromasil C18 column(4.6 mm×250 mm,5μm)at 30℃,with methanol-0.4%glacial acetic acid(55︰45)as the mobile phase at a flow rate of 1.0 mL.min–1 and a detection wavelength of 361 nm.The correlation coefficients ranged between 0.9990 and 0.9996 with calibration curves in the range of 0.001–0.01 mg·mL–1 for the four flavones.The average recoveries(n=9)obtained were 82.39%–99.03%.This method is simple,rapid,and efficient,and can be used for the simultaneous determination of rutin,quercetin 3-β-D glucoside,narcissoside,and quercetin in Suaeda Heteroptera samples. |
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| Keywords: | Suaeda Heteroptera RP-HPLC flavonoids rutin quercetin 3-β-D-glucoside narcissoside quercetin |
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