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大型海藻无性系微球体构建和反应器培养研究
引用本文:王金霞,李爱芬,周百成.大型海藻无性系微球体构建和反应器培养研究[J].海洋科学,2011,35(2):17-21.
作者姓名:王金霞  李爱芬  周百成
作者单位:1. 中国科学院海洋研究所,山东青岛,266071;中国科学院研究生院,北京100039
2. 暨南大学水生生物研究中心,广东广州,510632
3. 中国科学院海洋研究所,山东青岛,266071
基金项目:广东省高校水体富营养化与赤潮防治重点实验室开放基金资助项目(JN2010-2)
摘    要:利用大型海藻细胞的全能性获得了蜈蚣藻(Grateloupia filicina)、多管藻(Polysiphonia urceolata)和孔石莼(Ulva pertusa Kjellm)的无性系材料,在此基础上分别构建了绒球状微球体、网状微球体和簇生状微球体3种微球体形式,这种微球体形式适合于大型海藻的生物反应器高密度...

关 键 词:海藻无性系  光生物反应器  微球体  高密度培养
收稿时间:2009/12/29 0:00:00
修稿时间:2010/12/17 0:00:00

Studies on developments of microspheres from macroalgal clones and cultivations in airlift photobioreactor
WANG Jin-xia,LI Ai-fen,ZHOU Bai-cheng.Studies on developments of microspheres from macroalgal clones and cultivations in airlift photobioreactor[J].Marine Sciences,2011,35(2):17-21.
Authors:WANG Jin-xia  LI Ai-fen  ZHOU Bai-cheng
Institution:WANG Jin-xia1,3,LI Ai-fen2,ZHOU Bai-cheng1(1.Institute of Oceanology,the Chinese Academy of Science,Qingdao 266071,China,2.Jinan University,Research Center of Hydrobiology,Guangzhou,510632,3.The Graduate School of the Chinese Academy of Sciences,Beijing 100039,China)
Abstract:Three macroalgal clones of Grateloupia filicina, Polysiphonia urceolata and Ulva pertusa, respectively, were obtained by the totipotency of marine algae. From them, three types of algal microspheres suitable for cultivation in a 10-L airlift photobioreactor were developed, which were flossy, netty and fascicular, respectively. The tufted microspheres of U. pertusa were cultivated in the photobioreactor to the biomass density of 5.81 g/L FW L, with the maximum growth rate of 0.36 g/(L?d)FW and specific growth rate of 0.103 d?1. The technology of high-density culture of macroalgae in photobioreactor will have wide applications, including seeding, mass cultivation, integrate-aquaculture, and manufacture of bioactive products.
Keywords:macroalgal clone  photobioreactor  microsphere  high-density cultivation
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