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1.
To investigate the tissue-specificities of isozymes and the genetic structure of wild spotted halibut (Verasper variegates) population, horizontal starch gel electrophoresis was performed on 45 individuals collected in part of the Yellow Sea. The
performances of 17 isozymes in 8 kinds of tissues or organs were screened preliminarily in a TC-7.0 buffer system. The results
showed that the screened isozymes displayed remarkable tissue-specificities. Finally, 14 enzymes (AAT, ADH, EST, GPI, G3PDH,
IDHP, LAP, LDH, MDH, MPI, PGDH, PGM, SDH and SOD) and 4 kinds of tissues (eye, skeleton muscle, liver and heart) were selected
for genetic analysis. Fourteen isozymes are encoded by 20 loci, and 9 of them are polymorphic. The polymorphic loci are AAT-1*.
GPI-2* G3PDH*, IDHP-1*, LDH*, MPI*, PGM-1*. PGM-2* and SDH*, and the proportion of polymorphic loci is 0.4500 (P0.99). The mean values of observed and expected heterozygosities are 0.027 8 and 0.026 5, respectively and the average effective
number of alleles is 1.067 5. 相似文献
2.
用聚丙烯酰胺垂直板不连续凝胶电泳法对湛江海域褐菖鲉(Sebastiscus marmoratus)心、肝、肾、性腺、肌肉、鳃、眼、脑等8种组织的12种同工酶(ADH、a-AMY、CAT、EST、GDH、LDH、MDH、ME、POD、PPO、SDH、SOD)进行分析。结果表明,12种同工酶均有明显的组织特异性。经筛选,对肝脏和眼的8种同工酶(ADH、EST、LDH、MDH、ME、POD、SDH、SOD)进行群体遗传结构分析,共记录了18个基因位点,其中呈多态性的基因位点有5个(Adh-1、Est-2、m-Pod-1、s-Pod-1、s-Sod-1),多态位点比例(P)为27.78%,平均有效等位基因数(Ae)为1.238 8,平均观测杂合度(Ho)值为0.1401。 相似文献
3.
采用聚丙烯酰胺梯度凝胶垂直平板电泳对湛江地区的光裸星虫(Sipunculus nudus)3种组织(肠组织、体腔液和体壁肌肉)12种同工酶(SOD、EST、MDH、LDH、ALP、AMY、ME、ACP、G6PDH、SDH、ADH、GPI)进行检测分析。结果表明:检测的12种同工酶中的7种酶(SOD、EST、MDH、LDH、ALP、AMY、ME)具有明显的组织特异性;GPI在组织中表达不稳定,未检测到其他4种同工酶(ACP、G6PDH、SDH、ADH)的活性;光裸星虫酶谱带数目少,酶谱构型单一。并说明了对其种群内遗传生化特性进行系统研究的必要性。 相似文献
4.
ZHENG Xiaodong ) * ZHAO Jianmin ) XIAO Shu ) WANG Rucai ) WANG Shidang ) ZHOU Weiwu ) ) The Key Laboratory of Mariculture Ministry of Education Ocean University of China Qingdao P..R.China ) Wendeng Fishery Technical Extension Station Weihai P..R.China 《中国海洋大学学报(英文版)》2004,3(1)
1 Introduction ThegoldencuttlefishSepiaesculenta,aneriticanddemersalspecies,livesinthedepthof 1 0 - 1 0 0meters,andsometimesinseabottomsand .Itiswidelydistrib utedfromcentralHonshutoVietnamandthePhilip pines .Thegoldencuttlefishisalsoanimportantobjectof… 相似文献
5.
Zheng Xiaodong Zhao Jianmin Xiao Shu Wang Rucai Wang Shidang Zhou Weiwu 《中国海洋大学学报(英文版)》2004,3(1):48-52
Thirty-nine isozymes in four tissues (mantle muscle, buccal bulb muscle, eye and liver) of Sepia esculenta were screened for enzymatic analysis using starch gel electrophoretic technique. Eighteen enzymes (G3PDH, LDH, MDH, MEP,
IDHP, PGDH, GRS, NP, AAT, CK, AK, EST, ALP, ACP, FBP, MPI, GPI and PGM) show strong activities and good convergence in zymogram.
They are proved to be suitable genetic markers in Sepia esculenta. Among the tissues used, mantle muscle is the best for electrophoretic analysis of isozymes. Eye and liver are fairly good
for some special enzymes, such as LDH, EST, MPI, etc. Twenty-six loci are detected. The proportion of polymorphic loci is 0.115 in the Qingdao sample and 0.153 in the Rizhao
sample (P<0.99). The mean values of the observed and expected heterozygosity per locus of Qingdao sample are 0.016 and 0.017, while
those of the Rizhao sample are 0.023 and 0.025 respectively. 相似文献
6.
ＩＮＴＲＯＤＵＣＴＩＯＮＴｈｅｃｕｔｔｌｅｆｉｓｈＳｅｐｉｅｌｌａｍａｉｎｄｒｏｎｉｄｅＲｏｃｈｅｂｒｏｎｅ,1 884 ,ａｌｓｏｃａｌｌｅｄＳ .ｊａｐｏｎｉｃａＳａｓａｋｉ,1 92 9,ｉｓｄｉｓｔｒｉｂｕｔｅｄｗｉｄｅｌｙｉｎＡｓｉａ,ｉｎｔｈｅｓｅａｏｆＨｏｎｓｈｕ (Ｊａｐａｎ)ｔｏｔｈｅｎｏｒｔｈ ,ＭａｌａｙｓｉａａｎｄｔｈｅＰｈｉｌｉｐｐｉｎｅＩｓｌａｎｄｓｔｏｔｈｅｓｏｕｔｈａｎｄＩｎｄｉａｎＯｃｅａｎｔｏｔｈｅｗｅｓｔ.Ｉｔｉｓａｎｉｍｐｏｒｔａｎｔａｎｄｖａｌｕａｂｌｅｆｉｓｈｅｒｙｒｅｓｏｕ… 相似文献
7.
Horizontal starch gel electrophoresis was used to investigate genetic structures of four populations (Dandong, Rizhao, Ningbo
and Guangzhou) of redlip mullet (Chelon haematocheilus). Fourteen loci of ten enzymes (G3PDH, IDHP, LAP, LDH, MDH, MPI, PGDH, PGM, SDH, and SOD) were identified. The proportions
of polymorphic loci varied from 0.07 to 0.14. The average observed and expected heterozygosities ranged from 0.02 to 0.05
and from 0.02 to 0.04 respectively among the 4 populations. The average number of efficient alleles of each locus varied from
1.02 to 1.06. The genetic distance among populations was from 0.0004 to 0.0021. The genetic distances between Dandong, Rizhao
and Ningbo populations were small, while those between Guangzhou population and other three populations were relatively high.
The result indicated the possible divergence of redlip mullet between the South China Sea and the other sea areas of China
due to geographic isolation. 相似文献
8.
Genetic Analysis of Four Populations of Redlip Mullet(Chelon haematocheilus) Collected in China Seas
MENG Wei) GAO Tianxiang) * and ZHENG Bing) ) Key Laboratory of Mariculture Ministry of Education Ocean University of China Qingdao P. R. China ) Shandong Ocean Exploration Center Qingdao P.R.China 《中国海洋大学学报(英文版)》2007,6(1):72-79
1 Introduction The redlip mullet, (Chelon haematocheilus) (Tem-minck et Schlegel, 1845), belongs to the family Mugili-dae. It is mainly distributed in the coastal areas of Korea, Japan and China (Wang et al., 2000). Because of the good adaptation to envir… 相似文献
9.
Screening of 46 putative enzyme-coding loci and 4 different kinds of tissues of Sepiella maindroni de Rochebrone, 1884 for enzymatic activities using starch gel electrophoretic technique proved that the 21 enzymes such as AAT, AK, ALP, AP, CK, DIA, ES, FBP, G3PDH, GPI, GRS,IDH, LDH, MDH, MEP, MPI, NP, PGDH, PGM, SOD and XO* , were active to Sepiella maindroni after being stained. The tissue exhibiting stable and clear bands was also determined. Among tissues tested, mantle muscle tissue was the best for electrophoretic survey of isozymes. Buccal bulb muscle, eye and liver were fairly good for some special enzymes, such as DIA, ES, MPI, NT, etc. 相似文献
10.
Horizontal starch gel electrophoresis was used to investigate the biochemical genetic structure of Chinese mittencrab Eriocheir sinensis and Hepu mitten crab E. hepuensis. Sixteen putative enzyme-coding loci were examined for forty-nineChinese mitten crabs and thirty-eight Hepu mitten crabs. Nine loci, AAT-1 *, AAT-2 *, G3PDH*, GPI*, IDHP-1 *, IDHP-2 *,MDH-1 *, MDH-2 * and PGM * , are polymorphic in Chinese mitten crab, and seven, AAT-1 *, AAT-2 *, GPI *, IDHP-1 *,MDH-1 *, MDH-2 * and PGM * , are polymorphic in Hepu mitten crab. The proportion of polymorphic loci and the expectedheterozygosity are 0.562 5 and 0.080 3 for Chinese mitten crab, and 0.437 5 and 0.075 4 for Hepu mitten crab. The Nei' s ge-netic distance between two species is 0.002 4. 相似文献
11.
采用垂直板聚丙烯酰胺凝胶电泳技术,对月鳢十种组织中乳酸脱氢酶（ＬＤＨ）和酯酶（ＥＳＴ）同工酶进行了比较研究,结果表明：月鳢组织内ＬＤＨ和ＥＳＴ具有明显的组织特异性,ＥＳＴ由２个座位编码,ＬＤＨ则由Ｌｄｈ－ａ、Ｌｄｈ－ｂ两个基因编码。 相似文献
12.
运用高pH不连续聚丙烯酰胺凝胶(PAGE)电泳技术分析了似刺鳊鮈胚胎、仔鱼发育过程中5种同工酶变化,并对成鱼的9种组织同工酶分布特征进行了检测。结果发现:除醇脱氢酶(ADH)外,其他4种酶(EST、LDH、MDH、SOD)在发育过程中均被检出,且呈现明显的阶段性特征;在成鱼的4种同工酶也存在明显的组织特异性。4种酶共检测到9个基因位点。 相似文献
13.
The patterns of isozyme variation throughout the life cycle of the giant tiger prawn, Penaeusmonodon were described for ten enzyme systems (G3PD, MDH, LGG, GPI, MPI, FBP, PGM, LDH,PGD, and PROT). The zymograms showh little variation in the number of isozymic loci expressed duringlarval development compared with results reported for other penaeid prawns. There were specific ontogenicpatterns at some loci. As larvae developed beyond the embryonic stage, there was tendency for intensityof staining and complexity of banding patterns to increase. 相似文献
14.
The patterns of isozyme variation throughout the life cycle of the giant tiger prawn,Penaeus monodon were described for ten enzyme systems (G3PD, MDH, LGG, GPI, MPI, FBP, PGM, LDH, PGD, and PROT). The zymograms showed little
variation in the number of isozymic loci expressed during larval development compared with results reported for other penaeid
prawns. There were specific ontogenic patterns at some loci. As larvae developed beyond the embryonic stage, there was a tendency
for intensity of staining and complexity of banding patterns to increase. 相似文献
15.
XU Jianpeng ZHANG Quanqi QI Jie and WANG ZhigangLaboratory of Genetics Breeding College of Marine Life Science Ocean University of China Qingdao P. R. China 《中国海洋大学学报(英文版)》2007,6(2):153-160
Tissue expression and stock variation of isozymes of stone flounder (Kareius bicoloratus) were analyzed with horizontal starch gel electrophoresis. For the fourteen enzymes assayed, 31 loci were recorded. The results indicated that all the isozymes ex- amined were obviously tissue-specific. The expressions of SOD*, GDH*, G3PDH-2* and ADH-2* were detected only in liver, SDH-1*, MDH-1* and ADH-1* only in muscle, and LDH-B* and LDH-C* only in eyes. In comparison, MDH-2*, GPI-3* and SDH-2* were detected in all tissues examined. Other loci examined were detected in a variety of tissues. Muscle and liver were se- lected to detect the isozyme variation of the two geographic stocks of Qingdao and Weihai, Shandong Province, China. The percent- ages of polymorphic loci (P0.99) were 29.17% and 25.00%, the observed heterozygosities (H0) were 0.028 ± 0.014 and 0.040 ± 0.019, and the expected heterozygosities (He) were 0.039 ± 0.017 and 0.052 ± 0.022 in Qingdao and Weihai stock, respectively. The coeffi- cient of gene differentiation (Fst) and genetic distance (D) between the two stocks was 0.012 and 0.0011, respectively, indicating that the genetic differentiation is low between them. Compared with other species of Pleuronectiformes, both the percentage of polymor- phic loci and the mean heterozygosity of K. bicoloratus were at a middle level. 相似文献
16.
The genetic diversity of wild and hatchery populations of half-smooth tongue sole Cynoglossus semilaevis, based on observation of amplified fragment length polymorphism (AFLP) was described. Two hundred individuals from four wild populations, Laizhou (LZ), Weihai (WH), Qingdao (QD), Rizhao (RZ), and one hatchery population, Mingbo (MB), were screened using eight different AFLP primer combinations. A total of 384 loci were screened in the five studied populations. 48.4%, 51.3%, 50.7%, 49.3% and 45.8% of these loci were polymorphic among the individuals tested in the LZ, WH, QD, RZ and MB populations, respectively. The number of polymorphic loci detected by single primer combinations ranged from 17 to 35. The average heterozygosity of the LZ, WH, QD, RZ and MB populations was 0.072, 0.093, 0.092, 0.090 and 0.063, respectively. The WH population showed the highest genetic diversity in terms of total number of AFLP bands, total number of polymorphic bands, average heterozygosity and percentage of low frequency (0-0.2) polymorphic loci among all the populations, while the LZ population was the lowest among the wild populations. Compared with the wild populations, the hatchery population showed a low genetic viability. 相似文献
17.
采用聚丙烯酰胺垂直板梯度凝胶电泳技术,对管角螺7种组织(外套膜、肌肉、肝脏、心脏、生殖腺和缠卵腺)的5种同工酶(EST、SOD、ME、MDH、LDH)进行了分析,结果表明:5种同工酶存在不同程度的组织特异性,SOD、EST、LDH在肝脏组织中有较高活性,而MDH、ME在心脏组织中活性较高。 相似文献
18.
Population genetics of the left-eyed flounder, Paralichthys olivaceus, including natural and cultured stocks distributed in the coastal waters near Qingdao of eastern maritime China, was analyzed in allozyme and RAPD. The results showed that among total 29 gene loci of 15 isozymes, 9 and 7 were po- lymorphic in natural and cultured stocks, respectively. The status of genetic diversity in P. olivaceus is low in terms of polymorphic loci in chi-square test and genetic departure index of Hardy-Weinberg equi- librium. More alleles in IDHP, CAT, GDH and Ldh-C allozymes were found in the fish, which could be used as markers in assortive breeding and distinguishing stock, population or species evolution. Total 88 and 86 RAPD bands ranging from 200 to 2 500 bp were recognized individually in average of 7.8–8.0 bands per primer. The genetic diversity in cultured stock is lower than that in natural ones showing an ob- viously decreasing genetic divergence. Therefore, effective countermeasures must be taken to protect ge- netic resources of marine cultured fishes. The 2 markers have their own pros and cons. Combining the 2 markers to investigate the genetic variation of populations is suggested. The results provide basic data of this flounder and they are useful for studying genetic improvement and genetic resources of the fish. 相似文献
19.
The AFLP(amplified fragment length polymorphism) technique was used to analyze and compare the genetic diversity of Tachypleus tridentatus from three south-eastern coastal sites of China(Pingtan,Hong Kong and Beihai).Eight pairs of primers generated 361 loci,including 285 polymorphic loci.The ratio of polymorphic loci was 96.97%.Nei's genetic diversity index was 0.420 8 and the Shannon information index was 0.607 5,both of which were higher than that reported for many other arthropods.These results show tha... 相似文献