共查询到13条相似文献，搜索用时 375 毫秒
1.
利用胸腔注射PHA及秋水仙索溶液。取头肾细胞以空气干燥法制片对星斑裸颊鲷进行了染色体核型研究。结果表明其染色体核型公式为2n=48,48t，NF=48．未发现有随体染色体、次缢痕及性染色体。其核型属特点符合典型的高位类群鱼类核型特征。 相似文献
2.
【目的】研究棕点石斑鱼(Epinephelus tukula,♀)与蓝身大斑石斑鱼(Epinephelus fuscoguttatus,♂)杂交F1代(下称"金虎斑")的染色体核型。【方法】采用头肾-PHA注射法制备金虎斑染色体,并分析其核型。【结果与结论】金虎斑二倍体染色体数为48,包括23对端部着丝粒染色体,1对亚中部着丝粒染色体,核型公式是2n=48=2sm+46t,染色体臂数(NF)为50。与其他石斑鱼核型相比,金虎斑染色体核型特点符合典型的高位类群鱼类核型特征;根据染色体臂数,金虎斑属于特化类群。 相似文献
3.
以近江牡蛎的担轮幼虫为材料,采用秋水仙素——低渗处理——空气干燥制片法制作染色体标本,对其核型进行分析,结果表明,近江牡蛎体细胞染色体数目2n=20,NF=40,核型公式为20m。 相似文献
4.
管角螺、细角螺的核型研究 总被引：2，自引：0，他引：2
以管角螺、细角螺鳃组织为材料,经过秋水仙素处理,空气干燥法制片,Giemsa染色,观察分析获得染色体核型。结果表明,管角螺核型公式为2n=60=44m+10sm+6t,染色体总臂数NF=114。细角螺核型公式为2n=42m+10sm+2st+6t,总臂数NF=112。两种螺的全套染色体中未发现次缢痕、随体,也无性染色体。 相似文献
5.
《广东海洋大学学报》2016,(3)
以洛氏鱥(Phoxinus lagowskii)肾组织为材料,采用腹腔注射植物血球凝集素(PHA)、秋水仙素和空气干燥法制备染色体标本,进行染色体组型分析。结果表明:洛氏鱥染色体为50条,核型公式为2n=50=24m+14sm+12t,臂数NF=88,未发现异型染色体、随体染色体和次缢痕。 相似文献
6.
合浦珠母贝两个种群染色体组型的分析 总被引：2，自引：0，他引：2
以北部湾和大亚湾两个地域的野生合浦珠母贝种群为研究对象，采用成贝的鳃组织．经空气干燥法制片进行染色体组型分析。结果表明两地种群的核型公式都为2N=28=16m 6sm 4st 2t．NF=50．但在染色体排序方面表现出较明显的群体差异。并与以往研究报道相比较．分析了不同的实验结果及可能产生差异的原因。 相似文献
7.
以北部湾和大亚湾两个地域的野生合浦珠母贝种群为研究对象，采用成贝的鳃组织，经空气干燥法制片进行染色体组型分析。结果表明两地种群的核型公式都为2N=28=16m+6sm+4st+2t，NF=50，但在染色体排序方面表现出较明显的群体差异。并与以往研究报道相比较，分析了不同的实验结果及可能产生差异的原因。 相似文献
8.
哲罗鱼(Hucho taimen)染色体组型与DNA含量分析 总被引：1，自引：0，他引：1
采用体内注射PHA和秋水仙素、肾细胞短期培养、常规空气干燥法制备哲罗鱼(Hucho taimen)的染色体。对其肾细胞染色体数目统计分析表明,哲罗鱼染色体组有84条染色体,核型公式为2n=18m+16sm+34st+16t,其染色体总臂数(NF)为118。采用流式细胞分析仪测定哲罗鱼的DNA含量,与鸡血细胞标准对照的比值为2.23±0.17,以鸡红细胞DNA含量2.30pg·N~(-1)计,则哲罗鱼的体细胞DNA含量为5.12pg·N~(-1)。哲罗鱼染色体数目和DNA含量体现为四倍体特征。 相似文献
9.
斜带石斑鱼与赤点石斑鱼的核型研究 总被引：7，自引：0，他引：7
采用PHA体内注射肾细胞直接制片法研究斜带石斑鱼和赤点石斑鱼的核型。结果表明 ,斜带石斑鱼的核型为 2n =4 8,2sm +46t ,NF =5 0 ;赤点石斑鱼的核型为 2n =4 8,5st+43t ,NF =4 8。两种鱼的 2n数相同 ,它们的核型特点符合典型的高位类群鱼类核型特征。 相似文献
10.
斜带石斑鱼与赤点石斑鱼的核型研究 总被引：4，自引：0，他引：4
采用PHA体内注射肾细胞直接制片法研究斜带石斑鱼和赤点石斑鱼的核型。结果表明，斜带石斑鱼的核型为2n=48，2sm 46t，NF=50；赤点石斑鱼的核型为2n=48，5st 43t，NF=48。两种鱼的2n数相同，它们的核型特点符合典型的高位类群鱼类核型特征。 相似文献
11.
花染色体组型分析及细胞核DNA含量的测定 总被引：2，自引：0，他引：2
采用PHA、秋水仙碱腹腔或背部肌肉注射,活体培养法,取花肾细胞制成悬浮液,用空气干燥法制片。经Giemsa染色,镜检细胞分裂相,统计结果为2n=50,核型公式为:2n=20m+12sm+10st+8t,NF=82。同时,取花肌肉和肝脏为材料,以鸡血细胞为DNA标准(2.30pg/2c),使用EPICS-XL型流式细胞仪测定了花细胞DNA含量,分别为2.219±0.055pg/2c和2.214±0.083pg/2c。 相似文献
12.
Three wild populations of Meretrix meretrix sampled from Dongxing, Beihai, and Shankou along the coast of Guangxi, China, were investigated with morphometry and karyometry. Six morphological indices （shell length, shell height, shell width, hinge length, total wet weight and shell weight） were measured. Differences in all morphological indices except hinge length were significant among the three populations （P 〈 0.05）. The mean values of these indices （except for the hinge length） in the Dongxing population were larger than those in the Beihai and Shankou populations, although the latter had the largest hinge length. The karyotype of the Beihai, Shankou and Dongxing samples had ten metacentric, six submetacentric, and three subtelocentric chromosome pairs. No significant difference was shown in the centromeric index values of the chromosomes in the populations （P〉0.05）. However, the order of metacentric, submetacentric and subtelocentric chromosome pairs was variable among the three populations. The results indicate a high level of inter-population variation in morphology and karyotype. 相似文献
13.
The family Sciaenidae is remarkable for its species richness and economic importance. However, the cytogenetic data available in this fish group are still limited, especially those obtained using fl uorescence in situ hybridization(FISH). In the present study, the chromosome characteristics of a sciaenid species, Argyrosomus amoyensis, were examined with several cytogenetic methods, including dual-FISH with 18 S and 5 S rDNA probes, and a self-genomic in situ hybridization procedure(Self-GISH). The karyotype of A. amoyensis comprised 2 n=48 acrocentric chromosomes. A single pair of nucleolar organizer regions(NORs) was located at the proximal position of chromosome 1, which was positive for silver nitrate impregnation(AgNO_3) staining and denaturation-propidium iodide(DPI) staining but negative for Giemsa staining and 4',6-diamidino-2-phenylindole(DAPI) staining, and was confi rmed by FISH with 18 S rDNA probes. The 5 S rDNA sites were located at the centromeric region of chromosome 3. Telomeric FISH signals were detected at all chromosome ends with dif ferent intensities, but internal telomeric sequences(ITSs) were not found. Self-GISH resulted in strong signals distributed at the centromeric regions of all chromosomes. C-banding revealed not only centromeric heterochromatin, but also heterochromatin that located on NORs, in interstitial and distal telomeric regions of specifi c chromosomes. These results suggest that the karyotype of Amoy croaker was relatively conserved and primitive. By comparison with the reported cytogenetic data of other sciaenids, it can be deduced that although the karyotypic macrostructure and chromosomal localization of 18 S rDNA are conserved, the distribution of 5 S rDNA varies dynamically among sciaenid species. Thus, the 5 S rDNA sites may have different evolutionary dynamics in relation to other chromosomal regions, and have the potential to be ef fective cytotaxonomic markers in Sciaenidae. 相似文献