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1.
To investigate the genetic variation and population structure of Pacific herring in the Yellow Sea and the genetic differentiation between the Yellow Sea and the Sea of Japan,fragments of 479-bp mitochondrial DNA control region were sequenced for 110 individuals collected from three different periods in the Yellow Sea and one locality in the Sea of Japan.High haplotype diversity and moderate nucleotide diversity were observed in Pacific herring.AMOVA and exact test of population differentiation showed no significant genetic differentiations among the three populations of the Yellow Sea and suggested the populations can be treated as a single panmictic stock in the Yellow Sea.However,a large and significant genetic differentiation(WST50.11;P50.00) was detected between the populations in the Yellow Sea and the Sea of Japan.The high sea water temperature in the Tsushima Strait was thought a barrier to block the gene exchange between populations of the two sea areas.The neutrality tests and mismatch distribution indicated recent population expansion in Pacific herring.  相似文献   

2.
Japanese flounder is one of the most important commercial species in China; however, information on the genetic background of natural populations in China seas is scarce. The lack of genetic data has hampered fishery management and aquaculture development programs for this species. In the present study, we have analyzed the genetic diversity in natural populations of Japanese flounder sampled from the Yellow Sea (Qingdao population, QD) and East China Sea (Zhoushan population, ZS) using 10 polymorphic microsatellite loci and cytochrome c oxidase subunit I (COI) sequencing data. A total of 68 different alleles were observed over 10 microsatellite loci. The total number of alleles per locus ranged from 2 to 9, and the number of genotypes per locus ranged from 3 to 45. The observed heterozygosity and expected heterozygosity in QD were 0.733 and 0.779, respectively, and in ZS the heterozygosity values were 0.708 and 0.783, respectively. Significant departures from Hardy-Weinberg equilibrium were observed in 7 of the 10 microsatellite loci in each of the two populations. The COI sequencing analysis revealed 25 polymorphic sites and 15 haplotypes in the two populations. The haplotype diversity and nucleotide diversity in the QD population were 0.746±0.072 8 and 0.003 34±0.001 03 respectively, and in ZS population the genetic diversity values were 0.712±0.047 0 and 0.003 18±0.000 49, respectively. The microsatellite data (F_st =0.048 7, P<0.001) and mitochondrial DNA data (F_st =0.128, P<0.001) both revealed significant genetic differentiation between the two populations. The information on the genetic variation and differentiation in Japanese flounder obtained in this study could be used to set up suitable guidelines for the management and conservation of this species, as well as for managing artificial selection programs. In future studies, more geographically diverse stocks should be used to obtain a deeper understanding of the population structure of Japanese flounder in the China seas and adjacent regions.  相似文献   

3.
利用随机扩增多态DNA(RAPD)技术分别分析了五岛西部日本鲐、东海西部日本鲐和澳洲鲐三群体的样本,共产生226个分子标记,其中143个为多态性标记,多态百分率达到63.3%,每条引物产生的平均标记数为7.29。五岛西部日本鲐、东海西部日本鲐和澳洲鲐群体内遗传相似度分别为0.949、0.877和0.936,而群体间遗传距离依次为:五岛西部日本鲐-东海西部日本鲐(0.2357)<东海西部日本鲐-澳洲鲐(0.3030)<五岛西部日本鲐-澳洲鲐(0.3528),五岛西部日本鲐与东海西部日本鲐个体在UPGMA系统树独立聚类为两个类群,初步支持东、黄海的日本鲐存在东海西部和五岛西部的两个种群的观点。Shannon多样性指数表明,其遗传多样性大小依次为东海西部日本鲐、澳洲鲐、五岛西部日本鲐。  相似文献   

4.
Aurelia spp. ephyrae have been reported to form blooms in sea cucumber aquaculture ponds in the Bohai and Yellow Seas. To identify the species, we carried out a genetic analysis of Aurelia spp. ephyrae and medusae based on mitochondrial 16 S rRNA gene. Samples offour Aurelia sp. ephyrae populations were collected in sea cucumber aquaculture ponds and samples offour Aurelia sp. medusae populations were collected in coastal waters. Using a BLASTn search, we found that both the ephyrae collected in the aquaculture ponds and medusae collected in coastal waters belong to Aurelia coerulea. Seventeen haplotypes were recovered from the 16 S rRNA gene. The overall haplotype diversity and nucleotide diversity of the 166 A. coerulea individuals were 0.686% and 0.329%, respectively, indicating high haplotype diversity and low nucleotide diversity. Moreover, the haplotype diversity of ephyrae populations were generally lower than that of medusae populations with close sampling points. The genetic differentiation between ephyrae populations collected in the sea cucumber aquaculture ponds and A. coerulea medusae collected in coastal waters was not significant, suggesting the ephyrae populations in the sea cucumber culture ponds were part of the same genetic group as the medusae populations in the coastal waters. Phylogeographic analysis of the 16 S rRNA region revealed that there was no significant correlation between the haplotypes and the geographic distribution of populations. Pairwise fixation index values showed significant genetic differentiation and limited gene flow between A. coerulea population of Weifang and other locations.  相似文献   

5.
INTRODUCTIONTheYellowSeaandtheEastChinaSea (ECS)aremarginalseasofthenorthwestPacificandhaveexpansivecontinentalshelves .TheuniqueandstrikingfeaturesoftheYellowSeaandtheECSarethattheyhavestrongtidalcurrent;aresubjecttostrongmonsooninfluence ;andreceiveinflowfromthebiggestriverinChina ,theChangjiangRiver ;andthatthefamouswesternboundarycurrent,theKuroshio ,passesthroughtheECS ,withitsbranchesintrudingupwardintothecontinentalshelfareas.Generallyspeaking ,thewaterexchangecapacityofthe…  相似文献   

6.
Extremely large accumulation of green algae Enteromorpha prolifera floated along China’coastal region of the Yellow Sea ever since the summer of 2008.Amplified Fragment Length Polymorphism (AFLP) analysis was applied to assess the genetic diversity and relationships among E. prolifera samples collected from 9 affected areas of the Yellow Sea.Two hundred reproducible fragments were generated with 8 AFLP primer combinations,of which 194 (97%) were polymorphic. The average Nei’s genetic diversity, the coefficiency of genetic differentiation (Gst), and the average gene flow estimated from Gst in the 9 populations were 0.4018, 0.6404 and 0.2807 respectively. Cluster analysis based on the unweighed pair group method with arithmetic averages (UPGMA) showed that the genetic relationships within one population or among different populations were all related to their collecting locations and sampling time. Large genetic differentiation was detected among the populations.The E. prolifera originated from different areas and were undergoing a course of mixing.  相似文献   

7.
Macrobenthos samples were collected from the Yellow and East China Seas in four seasons during 2011 to 2012. The seasonal distribution of macrobenthos and its relationship with environmental factors were analyzed. A total of 562 macrobenthic species were identified, with polychaetes and mollusks accounting for 67% of the total number of species. A similarity percentage(SIMPER) analysis showed that the dominant species were bivalve mollusks in the Yellow Sea and small-sized polychaetes in the East China Sea. A two-factor analysis of variance showed significant seasonal variations in species number, density and diversity index, and significant regional differences of biomass and density. Two-factor community similarity analysis also showed significant seasonal and regional differences in macrobenthic communities. Canonical correspondence analysis indicated that the main environmental factors af fecting the macrobenthic communities were water depth, temperature, dissolved oxygen, and inorganic nitrogen. The results demonstrate significant regional differences and seasonal variations in macrobenthos in the two seas. Sediment properties and water mass characteristics are speculated to be the causes of regional differences.  相似文献   

8.
Oplegnathus fasciatus(rock bream) is a commercial rocky reef fish species in East Asia that has been considered for aquaculture.We estimated the population genetic diversity and population structure of the species along the coastal waters of China using fluorescent-amplified fragment length polymorphisms technology.Using 53 individuals from three populations and four pairs of selective primers,we amplified 1 264 bands,98.73% of which were polymorphic.The Zhoushan population showed the highest Nei's genetic diversity and Shannon genetic diversity.The results of analysis of molecular variance(AMOVA) showed that 59.55% of genetic variation existed among populations and 40.45% occurred within populations,which indicated that a significant population genetic structure existed in the species.The pairwise fixation index F_(st) ranged from 0.20 to 0.63 and were significant after sequential Bonferroni correction.The topology of an unweighted pair group method with arithmetic mean tree showed two significant genealogical branches corresponding to the sampling locations of North and South China.The AMOVA and STRUCTURE analyses suggested that the O.fasciatus populations examined should comprise two stocks.  相似文献   

9.
A time-dependent, three-dimensional finite difference model is presented for simulating the stratifiedYellow Sea and northem East China Sea. The mode is forced by time-dependent observed wind, surfaceflux of heat, and tidal turbulence. With this model, momentum and temperature distribution can be computed,and an approximation for the sub-grid scale effects is introduced by the use of mass and momentumexchange coefficients. The vertical exchanges are quite dependent on these assumed coefficents, whichare complicated functions of the turbulence energy of tide and wind, of the stratified strength and otherfactors. This model was applied to describe the mechanics of the variations in strength and thickness ofthe thermocline covering almost the whole Yellow Sea and northern East Chna Sea in summer. Comparisonsof the computed output with obtained survey data led to some important conclusions.  相似文献   

10.
Otolith shape is species specific and is an ideal marker of fish population affiliation. In this study, otolith shape of spottedtail goby Synechogobius ommaturus is used to identify stocks in different spawning locations in the Yellow Sea. The main objectives of this study are to explore the potential existence of local stocks of spottedtail goby in the Yellow Sea by analysis of otolith shape, and to investigate ambient impacts on otolith shape. Spottedtail goby was sampled in five locations in the Yellow Sea in 2007 and 2008. Otoliths are described using variables correlated to size (otolith area, perimeter, length, width, and weight) and shape (rectangularity, circularity, and 20 Fourier harmonics). Only standardized otolith variables are used so that the effect of otolith size on the shape variables could be eliminated. There is no significant difference among variables of sex, year, and side (left and right). However, the otolith shapes of the spring stocks and the autumn stocks differ significantly. Otolith shape differences are greater among locations than between years. Correct classification rate of spottedtail goby with the otolith shape at different sampling locations range from 29.7%–77.4%.  相似文献   

11.
Eleutheronema rhadinum is a potential commercial fisheries species and is subject to intense exploitation in China. Knowledge on the population structure of E. rhadinum in Chinese coastal waters, which is important for sustainable exploitation and proper resource management, is lacking. In the present study, the genetic diversity and population structure of E. rhadinum were evaluated using a 564-base pair fragment of the mitochondrial cytochrome c oxidase subunit I （COl） gene. A total of 76 specimens were collected from three localities around the East （Qidong and Zhoushan） and South China Seas （Zhuhai）. Among these individuals, nine polymorphic sites were detected and 1 l distinct haplotypes were defined. High levels ofhaplotype diversity （h=0.759i0.035） and low levels of nucleotide diversity （re=0.001 98i0.003 26） were observed in these populations, Hierarchical analysis of molecular variance （AMOVA） indicated that 96.72% of the genetic variation occurred within the populations, whereas 3.28% occurred among populations. No significant genealogical branches or clusters were recognized on the neighbor-joining tree. Intra-group variation among populations was significant （~0~t=0.032 85, P〈0.01）. These results suggest that E. rhadinum populations in the East and South China Seas have developed divergent genetic structures. Tests of neutral evolution and mismatch distribution suggest that E. rhadinum may have experienced a population expansion. The present study provides basic information for the conservation and sustainable exploitation of this species.  相似文献   

12.
Relationships between phytoplankton community composition and environmental variables in the East China Sea (ECS) and Yellow Sea (YS) were investigated using geochemical and molecular microbiology methods. The diversity of phytoplankton was characterized using cultivation-independent PCR-based denaturing gradient gel electrophoresis (DGGE). Groups resulting from unweighted pair-group method with arithmetic averages clustering of the DGGE profiles showed good consistency with the eco-environmental characteristics of the sea area they belonged to. Additionally, the clustering results based on DGGE fingerprinting and those based on morphological compositions were practically identical. The relationship of phytoplankton diversity to environmental factors was statistically analyzed. Temperature, dissolved inorganic nitrogen (DIN), and silicate-Si were found significantly related to the phytoplankton community composition. Canonical correspondence analysis (CCA) was performed to reveal the relationship between community composition and these three environmental factors. Generally, values of the ECS are clearly separated from those of the YS in the CCA biplot, due to mainly the effect of temperature and DIN.  相似文献   

13.
Oplegnathus fasciatus (rock bream) is a commercial rocky reef fish species in East Asia that has been considered for aquaculture. We estimated the population genetic diversity and population structure of the species along the coastal waters of China using fluorescent-amplified fragment length polymorphisms technology. Using 53 individuals from three populations and four pairs of selective primers, we amplified 1 264 bands, 98.73% of which were polymorphic. The Zhoushan population showed the highest Nei’s genetic diversity and Shannon genetic diversity. The results of analysis of molecular variance (AMOVA) showed that 59.55% of genetic variation existed among populations and 40.45% occurred within populations, which indicated that a significant population genetic structure existed in the species. The pairwise fixation index F st ranged from 0.20 to 0.63 and were significant after sequential Bonferroni correction. The topology of an unweighted pair group method with arithmetic mean tree showed two significant genealogical branches corresponding to the sampling locations of North and South China. The AMOVA and STRUCTURE analyses suggested that the O. fasciatus populations examined should comprise two stocks.  相似文献   

14.
Seasonal and annual with stress fields over the Bohai Sea, the Yellow Sea and the East China Sea were computed from the wind rose data compiled in the Climatic Atlas of Chinese Offshore Areas and North-west Pacific and published by the Ocean Press in 1982. 684 wind roses in 2° latitude by 2° longitude boxes constructed from 278,815 wind reports are involved in the present study. The computations are principally intended as a data source for further research. Some oceanographic consequences are expounded on.  相似文献   

15.
东海中部夏季鱼类群落结构及其多样性分析   总被引：2，自引：0，他引：2  
根据2008年8月东海区主要渔场重要渔业资源调查资料,分析东海中部鱼类的种类组成、区系特征、多样性等群落结构特征。结果表明:共调查捕获鱼类88种,其中暖水种59种,占67.0%;暖温种29种,占33.0%。相对重要性指数(IRI)值大于500的优势种鱼类共有5种,分别为带鱼、刺鲳、小黄鱼、六斑刺鲀和鳄齿鱼;种类丰富度指数变动范围为0.10～2.31,平均为0.88;Shannon-Wiener多样度指数变动范围为0.01～1.86,平均为0.87;种类均匀度指数变动范围为0.01～0.92,平均为0.41;东海中部夏季鱼类多样性指数与往年相比有下降的趋势。  相似文献   

16.
Diversion of the Yellow River is a unique geological event in offshore China, causing changes of the sedimentary environment in eastern China Seas. The last diversion took place in AD 1855, with the estuary diverted from the Yellow Sea into the Bohai Sea. The identification of the river diversion events in the shelf sediments would not only provide the definite ages for the sediments, but also give a clue for better understanding of the sedimentation in that area. In this study, 210 Pb, grain size, geochemical element, and foraminiferal data in core H205 from the north Yellow Sea were systematically investigated. A high-resolution sedimentary record was established, which was coupled with the Yellow River diversion and runoff changes. The results show that the foraminiferal composition and foraminiferal abundance of the sediments from the north Yellow Sea had good response to the Yellow River diversion in 1855. Before the change, shallow water assemblages dominated the foraminifera, and the abundance of each foraminiferal species was very low. After the diversion event, the abundance of most foraminifera increased sharply, with a maximum increase of 16 times, and the assemblage was still dominated by shallow water species. Furthermore, the changes in foraminiferal abundance in the core sediments corresponded well with the discharge fluctuation of the Yellow River since 1855. When the Yellow River began entering the Bohai Sea, the Yellow River water, which is rich in nutrients, along with the coastal currents affected the north Yellow Sea, increased the primary productivity in the north Yellow Sea, which is the main reason for the abrupt increase and fluctuation of foraminiferal abundance in this area. At the meantime, the East Asian winter monsoon could also promote the development of nearshore foraminiferal species by enhancing the coastal currents.  相似文献   

17.
Fatty acids(FAs) in sediment collected from three different stations in the Yellow Sea and the East China Sea were analyzed for their distributions and to determine evidence of harmful algal blooms in the sediment core.Less diverse FAs were found in the Yellow Sea(YS) station,whereas in the two stations of the East China Sea(ECS) the FAs were more diverse.Concentrations of some FA species in the two ECS stations displayed an occasional surge in their vertical profile.The highest concentration of FAs was found in the surface layer of station QT3(43.28 μg g~(-1)).Monounsaturated FAs were more susceptible to degradation compared to their saturated counterparts,and changes of 16:0,18:2 and 20:5 accounted for the most variability in total concentrations of FAs as those species made up most of the quantified FAs.The origins of the major fraction of FAs were attributed to autotrophic sources,and bacterial FAs accounted for only a small fraction of the total FAs in the region.Nutrient availability was a possible regulating factor controlling bacteria abundances in marine sediments in the ECS and the YS.A principal component analysis(PCA) was applied to analyze the FA dataset and to reveal the principal environmental factors that control the composition of FAs in the sediments.PC2,which explains 15% of the variance,was estimated to reflect the diagenetic effects on the FA compositional changes in sediments influenced by bacterial degradation.  相似文献   

18.
Journal of Oceanology and Limnology - The Yellow Sea (YS) and East China Sea (ECS) are important marginal seas of the western Pacific. Understanding the dynamics of methane (CH4) in the YS and ECS...  相似文献   

19.
Intraspecific diversity of molluscan species is usually studied based on maternally inherited mitochondrial DNA,from which only part of the evolutionary history can be reflected.Some nuclear ribosomal RNA genes such as 28S rRNA represent poten-tial candidates that can be easily applied in phylogeography because of lacking intraindividual variation.However,considering their low polymorphism,genetic appraisals on whether and how they can be used in population studies are necessary.Here,we applied a short 28S rRNA to assess genetic patterns of the clam Cyclina sinensis along the coast of China and compared the results with a for-mer study based on COI and ITS-1 analyses.The results revealed the 28S rRNA data set was characterized by an extremely low level of variation,with only seven haplotypes defined for 93 individuals.Haplotype and nucleotide diversity for each population was al-most the lowest when compared with the other two markers.However,the distribution of two dominant haplotypes showed clear geo-graphic patterns,and significant population differentiation was revealed between the East China Sea and the South China Sea.These patterns were highly concordant with findings of the former study that populations of C.sinensis were historically separated by land bridges among sea basins.Our study suggested that although the nuclear rRNAs have shortcomings such as low variation,they have advantages including lack of intraindividual variation and high amplification rates.Applying rRNA genes can enrich the toolbox of nuclear markers in molluscan phylogeographic studies.  相似文献   

20.
We use the particle size of sediments in core YS01 A to study the sedimentary environment of the mud deposit in the central South Yellow Sea of China during late Marine Isotope Stages 3(MIS3;40.5 kyr–31.3 kyr).In addition,the East Asian Monsoon and its relationship with the North Atlantic Ocean climate change are discussed based on the sensitive grain-size calculation and the spectrum analysis.The results show that during late MIS3,the muddy area in the central South Yellow Sea experienced the evolution of coastal facies,shallow marine facies,coastal facies,and continental facies,with weak hydrodynamic conditions.Compared with other climate indicators,we found that there were many century to millennium-scale climate signals documented in the muddy area sediments in the central South Yellow Sea.According to our particle size results,three strong winter monsoon events occurred at 37.6 kyr,35.6 kyr and 32.2 kyr.The East Asian Winter Monsoon records in core YS01 A are consistent with the Greenland ice core and the Hulu cave stalagmite δ~(18)O.The millennial and centennial scale cycles,which are 55 yr,72 yr,115 yr,262 yr respectively,correspond to solar activity cycles,while the 1049 yr and 2941 yr cycles correspond to the Dansgaard-Oeschger cycles.These cycles indicate that the paleoclimate evolution of the area was controlled by the solar activities,with the high-latitude driving thermohaline circulation as the main energy conveyor belt,followed by the sea-air-land amplification of the winter monsoon variation in the central Yellow Sea in the late MIS3.  相似文献   

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