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1.
The extensive spread ofPhragmites australis throughout brackish marshes on the East Coast of the United States is a major factor governing management and restoration
decisions because it is assumed that biogeochemical functions are altered by the invasion. Microbial activity is important
in providing wetland biogeochemical functions such as carbon and nitrogen cycling, but there is little known about sediment
microbial communities inPhragmites marshes. Microbial populations associated with invasivePhragmites vegetation and with native salt marsh cordgrass,Spartina alterniflora, may differ in the relative abundance of microbial taxa (community structure) and in the ability of this biota to decompose
organic substrates (community biogeochemical function). This study compares sediment microbial communities associated withPhragmites andSpartina vegetation in an undisturbed brackish marsh near Tuckerton, New Jersey (MUL), and in a brackish marsh in the anthropogenically
affected Hackensack meadowlands (SMC). We use phospholipid fatty acid (PLFA) analysis and enzymataic activity to profile sediment
microbial communities associated with both plants in each site. Sediment analyses include bulk density, total organic matter,
and root biomass. PLFA profiles indicate that the microbial communities differ between sites with the undisturbed site exhibiting
greater fatty acid richness (62 PLFA recovered from MUL versus 38 from SMC). Activity of the 5 enzymes analyzed (β-glucosidase,
acid phosphatase, chitobiase, and 2 oxidases) was higher in the undisturbed site. Differences between vegetation species as
measured by Principal Components Analysis were significantly greater at the undisturbed MUL site than at SMC, and patterns
of enzyme activity and PLFAs did not correspond to patterns of root biomass. We suggest that in natural wetland sediments,
macrophyte rhizosphere effects influence the community composition of sediment microbial populations. Physical and chemical
site disturbances may impose limits on these rhizosphere effects, decreasing sediment microbial diversity and potentially,
microbial biogeochemical functions. 相似文献
2.
湖北清江和尚洞滴水微生物群落随空间和季节的变化 总被引：2，自引：0，他引：2
为了考察现代微生物群落对环境因子的响应关系及其在古气候重建中的应用潜力, 利用微生物学传统的培养计数、荧光染色总菌计数和BIOLOG的ECO微平板等技术对湖北省清江和尚洞内不同地点的滴水由内至外进行了空间和季节性的动态监测, 4个滴水点由内向外编号依次为1D、3D、4D和5D.结果证实湖北清江和尚洞洞穴滴水中存在一定数量的微生物.不同样点微生物的代谢活性不同, 1D样品中的微生物群落代谢活性最高, 3D表现出与1D相似的代谢特征, 但平均颜色变化率(AWCD: 微生物对碳源的利用程度, 颜色变化率越高, 表明微生物代谢越旺盛)较1D小, 4D和5D样品在接种24h后平均颜色变化率(AWCD)基本保持不变.对微生物代谢较为旺盛的1D、3D两个样品中不同时间内微生物群落的多样性指数进行分析, 表明洞穴滴水中微生物多样性相对稳定, 随采样季节的变化不大.相对于3D、1D的4个不同季节(采样时间)微生物群落对碳源种类的利用上存在一定差异.1D水样在2006秋季(2006年11月份)、2007春季(2007年5月)和2009年夏季(2009年7月份)微生物群落利用碳源种类上比较接近, 而在2008年初秋(2008年9月)碳源利用种类与前三者的差异明显.与1D相比, 3D的4个不同采样时间的滴水微生物群落在主成分1和主成分2的得分系数比较接近, 群落碳源代谢功能多样性差别不大.这种变化可能反映了不同季节滴水中可溶有机质成分(DOC)的变化及微生物群落成分的变化, 暗示了微生物群落结构变化在指示气候变化应用中的巨大潜力.但微生物群落多样性与代谢功能多样性与气候因子之间的关系尚需深入研究. 相似文献
3.
Microbial diversity in the hydrate-containing (sites SH3B and SH7B) and-free (sites SH1B, SH5B, SH5C) sediments collected from the Shenhu area of the South China Sea (SCS) was investigated using 16S rR... 相似文献
4.
Damian M. Menning William A. Carraher-Stross Elaina D. Graham Devon N. Thomas Annie R. Phillips Robert J. Scharping James R. Garey 《Estuaries and Coasts》2018,41(2):430-443
Karst estuaries are unique systems governed by freshwater inputs that flow directly to the sea through karst conduits and/or matrices. Because they are found at the interface between terrestrial and marine environments, they can act as sentinels to climate change and as indicators of aquifer health. The physical and geochemical gradients formed in karst estuaries are a direct result of the interactions between inland hydrological conditions and sea level. To examine the influence these gradients have on microbial communities in the water column and sediment of a karst estuary, we studied the spring-fed Double Keyhole Karst Estuary in west central Florida for a 2-year period. Four sites were monitored within this system starting at the Double Keyhole Spring Conduit extending 2 km west through the estuary toward the Gulf of Mexico. Water column and sediment samples were collected quarterly at all sites from September 2011 through September 2013. Archaeal, bacterial, and microbial eukaryote communities were analyzed using quantitative PCR and length-heterogeneity PCR. The biological communities were analyzed in the context of hydrological, physical, and geochemical parameters in order to determine which factor(s) governed the observed changes of microbial abundance and richness. The major finding of this study was that microbial community change in this karst estuary was primarily driven by the volume of aquifer discharge and associated physical gradients, and less by the geochemical fluctuations within the system. 相似文献
5.
Chen Liu JianMing Xu NengFei Ding QingLin Fu Bin Guo YiCheng Lin Hua Li Ningyu Li 《Environmental Earth Sciences》2013,69(1):151-159
Reclamation of saline soil plays an important role in supporting high population growth in China. To evaluate the effects of reclamation and sustainability of salt-affected land production, soil chemical properties, enzyme activities, microbial community structure and function in sites reclaimed in 1976, 1984 and 1996 were characterized. The 2009 site was left to succession fallow and chosen as a control site. Results showed that electrical conductivity (EC) and pH decreased rapidly after the soil forming process started. An accumulation of soil organic C as a result of farming was observed. In all sites, inorganic N and available P were increased within 33 years after reclamation and commencement of agriculture. As a result of reclamation, soil enzyme activity (β-glucosidase, phosphatase, urease and arylsulfatase) was increased. There were no significant differences in soil enzyme activity between the sites reclaimed in 1976 and 1984, with the exception of phosphatase. Carbon source utilization patterns were less diverse in control soil than in treatment sites. The activities of reclamation resulted in synthesis of new phospholipid fatty acids (14:0, 17:1 c9, 16:0 2OH, 17:0 10Me, i17:0, 20:4 ω6c). Principal component analyses showed that the sites reclaimed in 1976 and 1984 clustered together and were distinct from 1996. Taken together, reclamation showed significantly increased soil quality and microbial activity. EC was the main limiting soil quality characteristic, which showed a comparative steady state after a reclamation time of 33 years. Differences in soil enzyme activity and microbial community function after long-term reclamation have potential to be reflected in soil functional integrity and ecosystem service. 相似文献
6.
冻融交替对不同年代排水造林湿地土壤微生物活性及有机碳密度的影响 总被引：5，自引：4，他引：1
以小兴安岭湿地土壤为研究对象,基于室内分析和冻融实验,分析了冻融作用下不同年代排水造林湿地土壤微生物量、酶活性以及有机碳密度的变化趋势,探讨了不同年代排水造林湿地土壤微生物活性与有机碳密度之间的相关关系,以期为深入认识冻融期间高寒高纬度地区土壤碳循环过程提供参考依据。结果表明：（1）冻融次数对土壤微生物量碳、氮含量影响显著（P<0.05）,经历9次冻融循环后,土壤微生物量碳、氮含量比冻融前明显减少;在三种不同年代排水造林的湿地中,排水时间越短,土壤微生物量碳、氮含量下降幅度越大,表明长时间的反复冻融交替可能导致土壤微生物量的进一步减少。（2）冻融前后,土壤蔗糖酶和淀粉酶活性均表现为下降趋势,且多次冻融交替后,-25~5℃冻融处理比-5~5℃冻融处理酶活性更低,表明较大的冻融温差更能降低土壤酶的活性度。（3）随着冻融次数和冻融温度的变化,四种湿地的土壤有机碳密度基本保持稳定,但其与土壤微生物量、酶活性却存在着高度的正相关性,通过探究微生物活性所调控的土壤过程,可以直接或间接了解土壤有机碳密度的变化趋势,便于从本质上验证其响应机制。 相似文献
7.
8.
J. William Schopf 《Precambrian Research》1977,5(2):143-173
Diverse, cellularly preserved microbial communities are now known from stromatolitic sediments of at least twenty-eight Precambrian formations. These fossiliferous deposits, principally cherts and cherty portions of carbonate units, range in age from Early Proterozoic (Transvaal Dolomite, ca. 2250 Ma old) to Vendian (Chichkan Formation, ca. 650 Ma old) and include units from Australia, India, Canada, South Africa, Greenland, the United States and the Soviet Union. More than three-quarters of these microbiotas have been discovered since 1970. Although few, therefore, have as yet been studied in detail, virtually all of the assemblages are known to be dominated by prokaryotic (bacterial and blue-green algal) microorganisms and to contain three major categories of microfossils: spheroidal unicells, cylindrical tube-like sheaths, and cellular trichomic filaments. Analyses of data now available (including measurements of more than 7800 fossil unicells) indicate that each of these three types of microfossils exhibited a gradual, but marked, increase in mean diameter and size range during the Proterozoic and that taxonomic diversity apparently also increased, especially beginning about 1400 Ma ago. Thus, it now seems evident that (i) the microbial components of Proterozoic stomatolitic assemblages have varied systematically as a function of geologic age and that (ii) such communities are both more abundant and more widespread than had previously been recognized. These observations augur well for the future use of such assemblages in Precambrian biostratigraphy. At present, however, data are sufficient to warrant the provisional establishment of only a few microfossil-based subdivisions of the Proterozoic. Such zones, necessarily relatively long-ranging, are here tentatively defined; it is of interest to note that boundaries between certain of these microfossil-based subdivisions appear to coincide, at least approximately, with previously suggested stromatolite-based boundaries. To some extent, therefore, results of this study seem consistent with, and may be supportive of, the concept of stromatolite-based biostratigraphy. At the same time, however, the study seems to indicate that stromatolites of markedly differing age, whether of similar or of dissimilar morphology, were probably formed by distinctly differing microbiotas. Data are as yet insufficient to indicate whether differing types of coetaneous, stratigraphically useful, stromatolites were formed by differing microbial communities and two what extent the “evolution” of stromatolite morphology was a result of the biologic evolution of stromatolite-building microorganisms. There is thus continued need for investigation of the potential biostratigraphic usefulness of stromatolitic microbiotas and, especially, for more effective integration of results of such studies with those available from studies of stromatolites without preserved microbiotas and from studies of the acritarchs preserved in Proterozoic shales. 相似文献
9.
Shang Wang Raymond M. Dong Christina Z. Dong Liuqin Huang Hongchen Jiang Yuli Wei Liang Feng Deng Liu Guifang Yang Chuanlun Zhang Hailiang Dong 《地学前缘(英文版)》2012,3(3):335-349
The Three Gorges Dam(TGD) of the Yangtze River.China,is one of the largest irrigation and hydroelectric engineering projects in the world.The effects of huge man-made projects like TGD on fauna and macrophyte are obvious,mainly through changes of water dynamics and flow pattern;however, it is less clear how microorganisms respond to such changes.This research was aimed to examine differences in microbial diversity at different seasons and locations(in front of and behind the TGD).In addition, differences between particle-attached and free-living communities were also examined.The community structures of total and potentially active microorganisms in the water columns behind and in front of the TGD were analyzed with the DNA- and RNA-based 16S rRNA gene phylogenetic approaches over three different seasons.Clone libraries of 16S rRNA genes were prepared after amplification from extracted DNA and.for some samples,after preparing cDNA from extracted rRNA.Differences were observed between sites at different seasons and between free-living and particle-attached communities.Both bacterial and archaeal communities were more diverse in summer than in winter, due to higher nutrient levels and warmer temperature in summer than in winter.Particle-attached microorganisms were more diverse than free-living communities,possibly because of higher nutrient levels and heterogeneous geochemical micro-environments in particles.Spatial variations in bacterial community structure were observed,i.e..the water reservoir behind the TGD(upstream) hosted more diverse bacterial populations than in front of the dam(downstream),because of diverse sources of sediments and waters from upstream to the reservoir.These results have important implications for our understanding of responses of microbial communities to environmental changes in river ecosystems affected by dam construction. 相似文献
10.
有机污染土壤-地下水系统中的微生物场及多场耦合研究 总被引：1，自引：0，他引：1
了解污染场地中微生物群落对有机污染物迁移转化的影响是高效修复治理有机污染场地的基础。自然界中的微生物以“场”的形式通过与水动力场、温度场、化学场等耦合作用,发挥其在维护生态稳定性及物质循环过程中的功能。微生物与有机物关联研究的文献计量分析结果显示,近年来关于微生物和有机物的研究主要集中在微生物群落结构、有机物的生物降解及有机物的迁移转化3个方面及一系列的关联研究中,而关于将微生物以“场”的形式定量刻画微生物在物质循环转化和维持生态稳定性方面作用的研究几乎没有。为此,本文通过研究给出了微生物场的定义,提出了有机污染场地中的微生物场概念模型和数学模型,分析了微生物场的主要影响因素及影响机制,构建了微生物场与水动力场、温度场、化学场之间耦合的本构模型。最后,对微生物场下一步的研究提出了展望。 相似文献
11.
Black smoker chimneys and biological vent communities have been identified at many sites on the deep seafloor, particularly along oceanic spreading centers. We report the largest and oldest known, microbe-rich sub-meter-sized black smoker chimneys and mounds from a 1.43 billion-year old sulfide deposit in a continental graben in northern China. These chimneys are especially well preserved, with characteristic morphology, internal textures and internal cylindrical mineralogical zonation. Four main types of chimneys are distinguished on textural and mineralogical criteria, exhibiting either Zn–Fe-sulfide or Pb–Zn–Fe-sulfide internal cylindrical mineralogical zones. The chimneys mark vent sites in submarine grabens indicating focused flow-venting processes. The fossil chimneys have mineralogical and geological evolutionary features similar to their counterparts on the modern seafloor and other submarine hydrothermal vents. Black smoker vent fluids and seafloor tectonism played important roles for formation of the massive sulfide deposits in the Mesoproterozoic.We also report the first known, remarkably diverse assemblage of fossil microbialites from around and inside Precambrian vent chimneys, demonstrating that Proterozoic life flourished around submarine hot vents and deep within the chimney vent passages. Filamentous, spherical, rod, and coccus-shaped fossil microbes are preserved preferentially on sulfide precipitates. Based on the depth and setting of the fossil biota, the organisms that produced the microbialites were likely sulfate-reducing chemosynthetic and thermophyllic microbes. Textural and mineralogical evidence shows that biomineralization processes enhanced chimney growth and sulfide precipitation.Close association of microorganisms with sulfide chimneys in modern deep-sea hydrothermal vents and younger ophiolites has sparked speculation about whether life may have originated at similar vents. However, little is known about fossil equivalents of vent microfossils and black smoker chimneys from Earth's early evolution. The fossilized microorganisms from the Gaobanhe black smoker chimney sulfide deposits include thread-like filaments with branching and twisted forms and preserved organic carbon, representing fossilized remnants of microbial mats metabolized at high temperatures characteristic of venting fluids. The preservation of fossil microorganisms provides evidence that microbial populations were closely associated with black smoker chimneys in Earth's early history. The microbial population clearly constitutes the site for mediating mineral formation. These ancient microbial fossils lead to a much better understanding of early life on the deep seafloor. The discovery of the Mesoproterozoic microfossils within black-smoker hydrothermal chimneys indicates that hydrothermal activity around sea-floor vents supported dense microbial communities, and supports speculation that vent sites may have hosted the origin of life. 相似文献
12.
Microbial diversity in waters,sediments and microbial mats evaluated using fatty acid-based methods 总被引：1，自引：0，他引：1
A. Mrozik A. Nowak Z. Piotrowska-Seget 《International Journal of Environmental Science and Technology》2014,11(5):1487-1496
The review summarises recent advances towards a greater comprehensive assessment of microbial diversity in aquatic environments using the fatty acid methyl esters and phospholipid fatty acids approaches. These methods are commonly used in microbial ecology because they do not require the culturing of micro-organisms, are quantitative and reproducible and provide valuable information regarding the structure of entire microbial communities. Because some fatty acids are associated with taxonomic and functional groups of micro-organisms, they allow particular groups of micro-organisms to be distinguished. The integration of fatty acid-based methods with stable isotopes, RNA and DNA analyses enhances our knowledge of the role of micro-organisms in global nutrient cycles, functional activity and phylogenetic lineages within microbial communities. Additionally, the analysis of fatty acid profiles enables the shifts in the microbial diversity in pristine and contaminated environments to be monitored. The main objective of this review is to present the use of lipid-based approaches for the characterisation of microbial communities in water columns, sediments and biomats. 相似文献
13.
青藏高原多年冻土区土壤需氧可培养细菌多样性及群落功能研究 总被引：7，自引：7，他引：0
以青藏高原腹地不同植被类型多年冻土区土壤细菌为研究对象, 分析了可培养菌群数量、 多样性和生理代谢功能的变化及其与环境因子间的关系. 结果显示: 从沼泽草甸到高寒荒漠, 土壤水分、 总碳、 总氮含量逐渐降低, pH值升高, 可培养细菌数量在2.97×106~2.88×107 CFU·g-1, 与含水量、 总碳、 总氮显著正相关; Actinobacteria(51.4%)和γ-Proteobacteria(31.7%)为优势菌群, α-protebacteria仅在沼泽草甸中有分布, β-protebacteria、 Bacterioidetes丰度与含水量、 总碳、 总氮间显著正相关; 自沼泽到荒漠, 菌群代谢活性和Shannon功能多样性指数降低, pH与Shannon指数显著负相关, 继氨基酸类碳源之后, 多聚物逐渐成为被细菌群落主要利用的碳源种类. 研究表明, 伴随冻土退化地上植被逆向演替的过程, 青藏高原多年冻土地下土壤微生物群落丰度、 遗传和代谢功能多样性均发生了不同程度的响应. 相似文献
14.
Semi-sealed preservation of soil samples at different moisture of 4% and 23%, respectively, was simulated to observe the variations of soil microbial communities and determine the contents and isotopic compositions of the total organic carbon and total nitrogen on the 7th and 30th day, respectively. The results show that during preservation, the quantity of microbial communities tended to increase first and then decrease, with a wider variation range at higher moisture (23%). At the moisture content of 23%, the microbial communities became more active on the 7th day, but less after 30 days, and their activity was stable with little fluctuation at the moisture content of 4%. However, there were no significant changes in the contents and isotopic compositions of the total organic carbon and total nitrogen. During preservation, the responses of soil microbes to the environment are more sensitive to changes in the total nitrogen and organic carbon contents. It is thus suggested that the variations of microbial communities have not exerted remarkable impacts on the isotope compositions of the total nitrogen and total organic carbon. 相似文献
15.
The relations among geochemical parameters and sediment microbial communities were examined at three shoreline sites in the Prince William Sound, Alaska, which display varying degrees of impact by acid-rock drainage (ARD) associated with historic mining of volcanogenic massive sulfide deposits. Microbial communities were examined using total fatty acid methyl esters (FAMEs), a class of compounds derived from lipids produced by eukaryotes and prokaryotes (bacteria and Archaea); standard extraction techniques detect FAMEs from both living (viable) and dead (non-viable) biomass, but do not detect Archaeal FAMEs. Biomass and diversity (as estimated by FAMEs) varied strongly as a function of position in the tidal zone, not by study site; subtidal muds, Fe oxyhydroxide undergoing biogenic reductive dissolution, and peat-rich intertidal sediment had the highest values. These estimates were lowest in acid-generating, intertidal zone sediment; if valid, the estimates suggest that only one or two bacterial species predominate in these communities, and/or that Archeal species are important members of the microbial community in this sediment. 相似文献
16.
We analyzed microbial diversity and community composition from four salt marsh sites that were impounded for 40?C50?years and subsequently restored and four unimpounded sites in southeastern Connecticut over one growing season. Community composition and diversity were assessed by terminal restriction fragment length polymorphism (TRFLP) and sequence analysis of 16S ribosomal RNA (rRNA) genes. Our results indicated diverse communities, with sequences representing 14 different bacterial divisions. Proteobacteria, Bacteroidetes, and Planctomycetes dominated clone libraries from both restored and unimpounded sites. Multivariate analysis of the TRFLP data suggest significant site, sample date, and restoration status effects, but the exact causes of these effects are not clear. Composition of clone libraries and abundance of bacterial 16S rRNA genes were not significantly different between restored sites and unimpounded sites, but restored sites showed greater temporal and spatial variability of bacterial communities based on TRFLP profiles compared with unimpounded sites, and variability was greatest at sites more recently restored. In summary, our study suggests there may be long-lasting effects on stability of bacterial communities in restored salt marshes and raises questions about the resilience and ultimate recovery of the communities after chronic disturbance. 相似文献
17.
互花米草入侵下福建漳江口红树林湿地土壤生态化学变化 总被引：1，自引：0，他引：1
采集了福建云霄漳江口米草入侵下红树林自然湿地保护区内红树林群落、米草群落、红树林—米草交互群落以及光滩的剖面土壤样品,分析了土壤的总养分库、有效养分库及微生物活性等指标。结果表明,不同植被群落下土壤理化性质均存在明显差异。土壤有机质、全氮、全磷、阳离子交换量和微生物生物量碳、氮在同一深度的含量变化为：红树林>红树林—米草混作>米草>光滩;土壤蔗糖酶、脲酶、磷酸酶、过氧化氢酶和多酚氧化酶等酶活性指标也表现出相似的变化趋势。从深度变化而言,上述各项土壤质量指标均随剖面深度而降低。米草入侵后土壤的各项养分指标均有明显下降,湿地土壤生态化学性质发生了明显的退化。土壤有机碳、土壤微生物生物量碳、氮含量以及土壤蔗糖酶与磷酸酶活性对滨海湿地土壤退化的反映最为强烈, 可以作为指示性指标。 相似文献
18.
Sang Shilei Dai Heng Hu Bill X. Huang Zhenyu Liu Yujiao Xu Lijia 《Hydrogeology Journal》2022,30(6):1833-1845
Microbes live throughout the soil profile. Microbial communities in subsurface horizons are impacted by a saltwater–freshwater transition zone formed by seawater intrusion (SWI) in coastal regions. The main purpose of this study is to explore the changes in microbial communities within the soil profile because of SWI. The study characterizes the depth-dependent distributions of bacterial and archaeal communities through high-throughput sequencing of 16S rRNA gene amplicons by collecting surface soil and deep core samples at nine soil depths in Longkou City, China. The results showed that although microbial communities were considerably impacted by SWI in both horizontal and vertical domains, the extent of these effects was variable. The soil depth strongly influenced the microbial communities, and the microbial diversity and community structure were significantly different (p < 0.05) at various depths. Compared with SWI, soil depth was a greater influencing factor for microbial diversity and community structure. Furthermore, soil microbial community structure was closely related to the environmental conditions, among which the most significant environmental factors were soil depth, pH, organic carbon, and total nitrogen.
相似文献19.
LIU Tung-Sheng 《《地质学报》英文版》2009,83(1)
Semi-sealed preservation of soil samples at different moisture of 4% and 23%,respectively, was simulated to observe the variations of soil microbial communities and determine the contents and isotopic compositions of the total organic carbon and total nitrogen on the 7th and 30th day, respectively.The results show that during preservation,the quantity of microbial communities tended to increase first and then decrease,with a wider variation range at higher moisture (23%).At the moisture content of 23%,th... 相似文献
20.
为了揭示季节性低温胁迫下陇东黄土高原油污土壤环境因子对耐冷混合菌场地生态修复的响应机制,利用自主筛选构建的耐冷石油降解混合菌在甘肃省庆阳市庆城县马岭镇长庆油田陇东油泥处理站开展了为期7个月的场地修复实验,采用常规方法测定了不同季节土壤理化特性、酶活性和微生物群落特性等环境指标。结果表明：（1）在季节性低温胁迫下（9-11月）M2组（耐冷混合菌处理组）月平均降解率明显增加（P<0.01）,JZJ+M1组（金盏菊联合常温混合菌处理组）和M2组累计TPH降解率分别为15.37%±3.51%和28.64%±4.12%。（2）M2组土壤脱氢酶和多酚氧化酶在低温季节（LT）活性最高,且温度和处理存在显著交互作用（P<0.01）。在土壤营养元素方面,无论何种处理方式（JZJ+M1和M2）二者含量均为RT（常温季节）高于LT（P<0.05）,同时明显高于CK组（P<0.05）。（3）M2组土壤微生物群落Shannon-Wiener指数和均匀度指数在LT高于RT（P<0.05）。（4）NMDS和Pearson相关性分析结果显示,M2组在低温季节具有较高TPH降解率主要与土壤多酚氧化酶、脱氢酶、单月TPH降解率（μ2）和Shannon-Wiener指数有关,且均呈极显著正相关关系。通过分析环境因子的季节响应,优化场地修复在低温环境的降解条件、加速低温期石油烃降解速率,以期为陇东地区低温耐冷混合菌场地生态修复技术的推广应用提供参考和基础数据资料。 相似文献