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1.
研究了米曲霉中性蛋白酶的特性。结果表明,米曲霉中性蛋白酶的最佳提取方法为加入pH 7.2的缓冲液研匀,置恒温(30℃)培养箱中提取30 min,每10 min搅动1次,最后用4层纱布过滤。酶反应最适温度为55℃,最适pH值为7.2。NaCl和EDTA对其有一定的抑制作用。酶的热稳定性好,在45℃的温度下处理24 h后,酶活力仍有45.5%。米曲霉中性蛋白酶与培养物一起保存时,稳定性也较好,在4℃保存一个月后,酶活力几乎无变化。  相似文献   

2.
罗非鱼溶菌酶活力的研究   总被引:5,自引:0,他引:5  
采用试管法(测定熔菌酶活力)研究pH和温度对罗非鱼血清,肝,鳃中溶菌酶活力的影响,以确定其最适pH和最适温度,同时研究了溶菌酶对温度的耐受性及其在体外的抑菌效果。结果表明,罗非鱼血清,肝溶菌酶的最适pH是6.30,最适温度是28℃,鳃中溶菌酶的最适pH是6.10,最适温度是34℃。各种酶在50℃保温40min以上将被灭活,在37℃以下活性是稳定的;体外抑菌试验表明罗非鱼溶菌酶对革兰氏阳性菌具有较好的抑菌效果。  相似文献   

3.
米曲霉产中性蛋白酶的适宜条件   总被引:3,自引:0,他引:3  
研究了米曲霉产蛋白酶的分布,优化了米曲霉产中性蛋白酶的适宜培养条件以及培养基的最优组成。研究发现米曲霉产中性蛋白酶的能力为最强。米曲霉产中性蛋白酶的适宜培养条件为:m(麸皮)∶m(豆粕粉)=4∶1,水的质量分数为60%,培养基中各无机盐质量分数为:KNO30.2%,MgSO40.05%,Na2HPO40.13%,pH值为6.0,接种量为每10 g培养基接种1.0×108个孢子,最佳培养温度为30℃,最佳培养时间为48 h。在此培养条件下,最高酶活力达3 999.2 U.g-1。  相似文献   

4.
几种因子对鞍带石斑鱼精子活力的影响   总被引:3,自引:0,他引:3  
研究不同梯度的盐度、pH、温度及低温保存时间对鞍带石斑鱼精子活力的影响。结果表明:鞍带石斑鱼精子活力的适宜盐度范围为27.5~35.0,最适盐度为32.5,此时精子的快速运动时间和寿命分别为13.2min和14.8min;最适pH为8.5,精子的快速运动时间及寿命最长,分别达12.7min和17.3min;温度范围为27~31℃,以29℃时快速运动时间最长,达16.6min;在0~4℃冰箱保存6h后鞍带石斑鱼精子活力未显著降低,其中保存前精子快速运动时间为18.4min,保存6h后为18.2min,保存120h后仍有活力,精子快速运动时间为2.3min,精子激活率为3.3%。以x为保存时间,快速运动时间y和寿命y′符合线性方程y=-0.8334x+19.164(R2=0.9856),y′=-1.1358x+27.8(R2=0.9573)。  相似文献   

5.
罗非鱼溶菌酶活力的研究   总被引:1,自引:0,他引:1  
采用试管法 (测定溶菌酶活力 )研究pH和温度对罗非鱼血清、肝、鳃中溶菌酶活力的影响 ,以确定其最适pH和最适温度 ,同时研究了溶菌酶对温度的耐受性及其在体外的抑菌效果。结果表明 ,罗非鱼血清、肝溶菌酶的最适pH是 6 3 0 ,最适温度是 2 8℃ ;鳃中溶菌酶的最适pH是 6 1 0 ,最适温度是 3 4℃。各种酶在 5 0℃保温 40min以上将被灭活 ,在 3 7℃以下活性是稳定的 ;体外抑菌试验表明罗非鱼溶菌酶对革兰氏阳性菌具有较好的抑菌效果。  相似文献   

6.
应用正交试验优选枯草杆菌中性蛋白酶和胃蛋白酶对青鳞鱼蛋白质的水解条件。结果表明:(1)枯草杆菌中性蛋白酶的最适条件为pH7.0、温度50℃、时间3h、酶浓度10,000μ/100ml;(2)胃蛋白酶的最适条件为pH3.5、时间2.0h、温度40℃、酶浓度300μ/100ml;(3)以去内脏青鳞鱼为原料进行双酶水解,蛋白质水解度可达83.8%。水解液经脱臭浓缩可制得a──氨基氮≥1.5g/100Inl的HAP。它可作为海鲜食品的添加剂,也可制作海鲜汤料或作为膨化食品的辅料。  相似文献   

7.
酶解法提取鱼油的工艺参数优化   总被引:1,自引:0,他引:1  
利用蛋白酶酶解法从黄鳍金枪鱼(Thunnus albacares)加工的下脚料——鱼头中提取鱼油。以鱼油提取率和感官特征为指标,通过正交优化实验设计,获得了胰蛋白酶提取鱼油的最佳酶解工艺参数:酶解温度45℃,酶添加量1.5%.料液质量比1:1,酶解时间4h,酶解pH8。在该条件下.鱼油的提取率为4.34%,理化指标除过氧化值外均达到SC/T3502-2000的粗鱼油二级标准。多不饱和脂肪酸总含量高达38.47%,其中DHA和EPA的含量分别为23.63%和4.84%。  相似文献   

8.
鲢鱼降血压肽的酶解条件   总被引:3,自引:0,他引:3  
以鲢鱼蛋白为原料,采用5种不同复合蛋白酶(P1,P2,P3,P4,P5)进行酶解。综合研究了复合生物酶酶量、酶解时间、pH、酶解温度等对鲢鱼蛋白酶解降血压肽ACE活性抑制效果的影响。结果表明。复合酶P1酶解降血压肽对ACE抑制率经体外检测为88.08%。具有最佳的酶解效果。对应最佳条件为:酶量25IU/mL,酶解时间2h,pH 7.0,酶解温度60℃。  相似文献   

9.
【目的】研究热处理温度和时间对凡纳滨对虾肌肉组织蛋白酶L酶活力的影响,并分析其酶活力变化动力学。【方法】从凡纳滨对虾的肌肉组织提取组织蛋白酶L,研究加热温度(35~60℃)和时间(5~60 min)对其酶活力的影响,采用反应动力学模型拟合酶活力的变化过程。【结果】在35℃组织蛋白酶L的酶活力变化适合用一级动力学模型来描述,而在40~60℃组织蛋白酶L的酶活力变化适合用两阶段动力学模型来描述;凡纳滨对虾肌肉组织蛋白酶L存在敏感型和稳定型两种同工酶,敏感型的Z_(T,L)和E_(a,L)分别为158.7℃和5.5k J/mol,稳定型的Z_(T, s)和E_(a, s)分别为71.9℃和12 kJ/mol。【结论】加热处理可以使组织蛋白酶L发生变性而失活,采用合理的加热温度和速度可以降低组织蛋白酶L引起的凝胶劣化。  相似文献   

10.
几株有益芽孢杆菌对温度、制粒工艺及pH值的耐受性   总被引:4,自引:0,他引:4  
报道了实验室分离、筛选的5株有益芽孢杆菌对温度、对虾饲料制粒工艺流程和pH值的耐受性。结果表明,5株芽孢杆菌经80℃水浴40min后全部存活,90℃水浴20min有35%-70%存活,100℃水浴10min仍有30%-50%存活,显示所筛选的5株芽孢杆菌对高温有较强的耐受力;在对虾饲料中添加5株芽孢杆菌,经整个生产工艺流程后芽孢杆菌存活95%,烘干后芽孢杆菌存活93%,说明杆菌能够承受饲料制粒生产中压力、温度和水分的变化;当pH值为3.8、4.6、5.2时,分别有l、3、5株菌能繁殖,pH值6.0-8.5时,5株菌生长旺盛,将5株菌经pH值2.2—4.6处理1h,再接种在pH值7.2的培养基可以良好生长,说明杆菌在酸性环境中生长性能较差,在弱酸性和碱性环境中生长旺盛;能耐受酸性环境而存活,再给予适宜环境即能良好生长。5株芽孢杆菌具有作为水产硬颗粒饲料专用益生素菌株的优势。  相似文献   

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12.
Based on the hydrographic data in austral summer during the 22nd Antarctic Expedition of China(2005/2006),some features can be found about the northern margin of Emery ice shelf as follows.The heat content in the surface layer(0-50 m) at the eastern end and the western end of the ice-shelf margin is much higher than that at the middle.The upper mixing-layer depth and the seasonal thermocline depth at the middle of the ice-shelf northern margin are much shallower than those at the both ends.However there is much less difference between the middle and the ends in the bottom layer.The remote sensing photos show that the inhomogeneity in the surface-layer water is closely related to the spatial distribution of the floes and polynia in the area.  相似文献   

13.
Phylogenetic analysis based on 16S rDNA of 8 strains of cultivable bacteria isolated from Arctic sea-ice was studied.The results showed that strain BJ1 belonged to genus Planococcus,which was a genus of low mole percent G C gram-positive bacteria;strain BJ6 belonged to genus Burkholderia of β-proteobacteria and the rest 6 strain all belonged to γ-proteobacteria,of which strain BJ8 was a species of Pseudoalteromonas,strain BJ2-BJ5 and BJ7 were members of genus Psychrobacter.Phylogenetic analysis also indicated that bacteria of genus Psychrobacter of the isolates formed a relatively independent phylogenetic cluster in comparison with other bacteria belonged to genus Psychrobacter.  相似文献   

14.
正1 Aims and Scope Advances in Polar Science(APS)is an international,peer-reviewed journal jointly sponsored by the Polar Research Institute of China and the Chinese Arctic and Antarctic Administration.APS is a comprchensive academic journal dedicated to presentation of multi-disciplinary achievements in Arctic and Antarctic expeditions and research.Its primary purpose is to publish achievements in fundamental research,applied  相似文献   

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16.
《山地科学学报》2014,(4):I0004-I0009
<正>AIMS AND SCOPE(Editorial Policy)The Journal of Mountain Science(JMS)is devoted to mountains and their surrounding lowlands-ecoregions of particular global importance,with a particular emphasis on the important highlands/mountains in the world,such as the Tibetan Plateau,the Himalayas,the Alps,the Andes,the Rockies and many other mountain ranges of our planet.  相似文献   

17.
<正>The Journal of Mountain Science(JMS),founded in2004,is an international English-language journal on mountain sciences.JMS is supervised by the Chinese Academy of Sciences(CAS),sponsored by the Chengdu Institute of Mountain Hazards and Environment,CAS,published by Science Press China,and distributed by Springer exclusively throughout the world(excluding Mainland China).The JMS is published bimonthly,fulltexted in SpringerLink and CNKI,indexed/abstracted by  相似文献   

18.
Using the NCEP/NCAR reanalysis monthly 500 hPa height data on a 2.5 latitude-longitude grid and 1°×1° sea ice data,the polar vortex area,intensity index and arctic sea ice area index are calculated respectively,and the meridional distribution,period variation and the abrupts in the long range trend are analyzed to study their relationship.The results show that the meridional distribution of sea ice and polar vortex h-ave distinctive difference,the relative positions of them are different in the eastern and western hemispheres,and exept they have periods of 4 months,quasi half year,quasi year,4-5 years and 10 years commonly,and each of them has its own respective variation as well.The sea ice area is decreasing apparently since 1980's,so is the polar vortex area,but their abrupt changge time are different totally.The area of sea ice and polar vortex has prominent positive correlation,but the relationship of sea ice intensity,polar vortex intensity,polar vortex area is complicated.  相似文献   

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<正>The Journal of Ocean University of China(Oceanic and Coastal Sea Research)is a comprehensive academic quarterly sponsored by the Ocean University of China,published one volume per year.This journal is devoted to the publication of the theoretical and applied research  相似文献   

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