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1.
Crustin 是目前报道广泛存在于甲壳类中的一类具有广谱抗菌活性的抗菌肽, 主要由甲壳类血淋巴细胞合成。本研究采用RACE 技术和真核表达技术, 进行了三疣梭子蟹Crustin 基因的克隆和重组表达研究。结果表明, 在三疣梭子蟹血细胞Crustin 基因cDNA 全序列中, 开放阅读框编码110个氨基酸残基的Crustin 前体, Crustin 前体由N 端21 个氨基酸残基的信号肽、富含Cys 结构域和C端WAP 结构域三部分组成, 属于I 型Crustin, 构建的分泌型真核表达质粒pVT102U/α-crustin 转化酿酒酵母S78, 经RT-PCR 和SDS-PAGE 电泳验证, 表明重组Crustin 表达成功。 相似文献
2.
《海洋与湖沼》2010,(3)
Crustin是目前报道广泛存在于甲壳类中的一类具有广谱抗菌活性的抗菌肽,主要由甲壳类血淋巴细胞合成。本研究采用RACE技术和真核表达技术,进行了三疣梭子蟹Crustin基因的克隆和重组表达研究。结果表明,在三疣梭子蟹血细胞Crustin基因cDNA全序列中,开放阅读框编码110个氨基酸残基的Crustin前体,Crustin前体由N端21个氨基酸残基的信号肽、富含Cys结构域和C端WAP结构域三部分组成,属于I型Crustin,构建的分泌型真核表达质粒pVT102U/α-crustin转化酿酒酵母S78,经RT-PCR和SDS-PAGE电泳验证,表明重组Crustin表达成功。 相似文献
3.
中国对虾素在大肠杆菌(Escherichia coli)中的重组表达 总被引：3，自引：0，他引：3
中国对虾素是从中国对虾血细胞中克隆得到的一种抗菌肽。为了进一步研究中国对虾素(CHP)的功能并为制备特异性抗体作准备，采用大肠杆菌表达外源蛋白的方法，进行了对虾素原核表达的研究。根据从中国对虾血细胞中克隆得到的对虾素基因设计特异性引物扩增中国对虾素成熟肽基因(CHP)，插入原核表达载体pGEX-4T-1中，在E．coli BL21表达融合蛋白GST-CHP．结果表明，不同表达菌株的融合蛋白表达量为30％—34％，同一菌株在诱导5h后能达到最高表达量。利用GST亲和层析纯化融合蛋白，将融合蛋白用凝血酶裂解以得到CHP，其分子量约为5．6kDa，N-端测序结果与期望的成熟对虾肽序列一致。用液体生长抑制方法检测活性，表明重组GST-CHP蛋白及CHP均表现出对大肠杆菌的抑菌活性。 相似文献
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5.
利用构建的SMART cDNA文库和高通量测序方法,得到了青蛤溶菌酶相关基因的全长,采用荧光定量RT-PCR方法分析了c型溶菌酶基因在鳗弧菌刺激下的表达变化。结果表明,青蛤有c型溶菌酶和i型溶菌酶基因,分别编码155和182个氨基酸,c型溶菌酶信号肽为21个氨基酸并具有典型的LYZ1结构域,i型溶菌酶信号肽为19个氨基酸,其结构域为Destabilase domain结构,用于识别和切断谷氨酰胺-甲酰胺与赖氨酸ε-氨基之间的肽键。荧光定量PCR结果显示,在鳗弧菌刺激后6—24h,青蛤血细胞中c型溶菌酶的表达量出现明显上调的趋势,与对照组有显著性差异(P0.05),说明c型溶菌酶基因在青蛤的免疫应答中起重要的作用。 相似文献
6.
利用RT-PCR和RACE技术获得了中国明对虾Rel／NF-κB家族成员FcRel基因的部分cDNA片段，并研究了该基因在弧菌刺激条件下的转录表达变化。结果表明，该cDNA编码320个氨基酸，包含一个完整的RHD结构域和部分IPT-NFkappaB结构域。FcRel基因的RHD结构域与其它物种的RHD结构域的分子系统学分析表明，中国明对虾的FcRel与果蝇的Relish聚在一起，然后与疟蚊、烟草天蛾等其它节肢动物的Relish聚为一类。在弧菌刺激时，FcRel在血细胞和淋巴器官中的转录表达显示出不同的变化模式。在弧菌注射后3h和5h，血细胞中FcRel的转录表达出现明显下调；而弧菌注射引起淋巴器官中FcRel基因明显的上调表达。说明FcRel基因的表达与弧菌刺激密切相关，提示Relish可能参与了对虾抗菌反应的信号转导通路。 相似文献
7.
采用RT-PCR和RACE-PCR技术克隆了大黄鱼(Larimichthys crocea)Ⅰ型干扰素基因全长cDNA序列及其基因组序列,并利用荧光定量PCR技术研究了该基因在大黄鱼不同组织中的表达谱,以及副溶血弧菌(Vibrio parahaemolyticus)、LPS、polyI:C刺激后大黄鱼脾脏、肝脏和头肾组织中该基因在转录水平的表达变化。结果表明,大黄鱼Ⅰ型IFN基因全长cDNA为878bp,开放阅读框558bp,编码185个氨基酸。推测的大黄鱼Ⅰ型IFN氨基酸序列N端含有20个氨基酸的信号肽,其后包含一个保守的IFabd结构域。大黄鱼Ⅰ型IFN基因包含5个外显子,4个内含子,在大多数组织和细胞中都有表达,其中在血细胞中表达量最高,肌肉中表达量最低。PolyI:C刺激可诱导大黄鱼Ⅰ型干扰素基因在脾脏、头肾和肝脏中的表达量显著上调;LPS刺激可诱导大黄鱼Ⅰ型干扰素基因在脾脏和肝脏中的表达量显著上调;灭活的副溶血弧菌可诱导大黄鱼Ⅰ型干扰素基因在头肾和肝脏中的表达量显著上调。 相似文献
8.
胰岛素样生长因子-I(Insulin-like growth factor-I,IGF-I)是影响脊椎动物生长、发育及代谢的重要调控因子。本研究采用RT-PCR和RACE技术,克隆了银鲳(Pampus argenteus)肝脏IGF-IcDNA序列,应用半定量RT-PCR、Real-time q PCR和原位杂交的方法检测了IGF-I的组织表达特性、在肝脏中的生长表达特性和IGF-I基因在肝脏中的定位。序列分析表明,IGF-I cDNA序列全长836bp,其5′非编码区128bp、3′非编码区92bp,开放阅读框(open reading frame,ORF)605bp,由此推导IGF-I前体蛋白由201个氨基酸组成;前体肽由信号肽、成熟肽、E肽三部分组成,其中信号肽59个氨基酸,成熟肽68个氨基酸,E肽74个氨基酸;成熟肽由B、C、A、D四个区域组成,E肽分析表明,银鲳IGF-I属Ea-4型。同源性比较结果表明,银鲳与同目鲈形目鱼类的IGF-I编码序列同源性较高,为83.52%—91.40%;与哺乳类、鸟类和爬行类的同源性较低。半定量RT-PCR和Real-time q PCR组织特异性表达结果显示,IGF-I m RNA在肝脏组织中的表达量最高,显著高于其它组织,肾脏、心脏、肌肉、脑、鳃、小肠、卵巢次之,嗅球、脾脏和胃中表达较低;半定量RT-PCR和Real-time q PCR不同生长阶段表达结果显示,IGF-I m RNA在30—50g肝脏组织中表达量最高(P0.05);IGF-I m RNA在肝脏中的原位杂交定位结果显示,在肝脏细胞中均有表达,阳性信号主要位于细胞质中,靠近细胞边缘处信号较强。 相似文献
9.
Hepcidin是一类具有独特性质的小分子抗菌肽,克隆得到大黄鱼(Pseudosciana crocea)的hepcidin基因(Lyc-Hepc),cDNA序列全长585个核苷酸,编码一个由85个氨基酸组成的蛋白,包含24个氨基酸的信号肽、35个氨基酸的前导肽和26个氨基酸的成熟肽,成熟肽具有8个保守的半胱氨酸.将Lyc-Hepc 183bp的编码前导肽和成熟肽的前体肽(25aa-85aa)基因片段克隆到毕赤酵母表达载体pPICZαA中,然后电击转化巴斯德毕赤酵母SMD1168,经高浓度Zeocin筛选获得多个单克隆.挑取4个克隆进行培养,提取酵母基因组进行PCR检测,结果表明目的基因片段已成功整合到酵母菌SMD1168基因组中,获得酵母工程菌株SMD1168/pP1CZα-Lyc-Hepc.SDS-PAGE和WesternBlot分析表明酵母工程菌株在0.5％甲醇诱导培养72h后,Lyc-Hepc在培养上清中得到了分泌型表达.体外抑菌实验表明重组Lyc-Hepc蛋白能够抑制2株水产养殖病害菌嗜水气单胞菌和副溶血弧菌的生长.研究结果表明大黄鱼hepcidin可能在抗细菌免疫中发挥了作用. 相似文献
10.
脊尾白虾(Exopalaemon carinicauda)组织蛋白酶D基因的克隆及其表达分析 总被引：2，自引：1，他引：1
采用RACE技术获得了总长为1853bp的脊尾白虾(Exopalaemon carinicauda)组织蛋白酶D基因全长cDNA序列。该基因5′和3′非编码区分别为120bp和572bp,开放阅读框为1161bp,推测编码386个氨基酸,预测分子量为42.36kDa,理论等电点为7.54,命名为EcCatD基因。同源性和系统进化分析表明,EcCatD基因与斑节对虾、美洲螯龙虾的相似性分别为86%和80%,与斑节对虾和美洲螯龙虾紧密聚为一支。荧光定量RT-PCR结果表明,EcCatD基因在血细胞中的相对表达量最高,其次为肝胰腺。该基因在鳗弧菌和WSSV感染后的脊尾白虾血细胞和肝胰腺中的表达量显著增加,并具有不同的时空表达趋势。结果表明EcCatD基因在脊尾白虾免疫反应中具有重要作用。 相似文献
11.
本研究采用解剖学及组织学方法对管角螺消化系统的结构进行了系统分析．结果表明,管角螺的消化系统由消化道和消化腺两大部分组成．消化道包含吻、食道、嗉囊、胃、肠、直肠及肛门;消化腺由唾液腺、肝及食道腺组成,其中肝为最大消化腺．唾液腺细胞分为粘液细胞和分泌细胞;食道腺细胞由排泄细胞和杯状细胞组成．在光学显微镜下,消化道的管壁由外向内依次为：外膜、肌层、粘膜下层和粘膜层4层;唾液腺、肝及食道腺均为复管状腺．管角螺的肝由肝被膜和许多盲管（肝小管）组成,肝管的组织结构与消化道基本相同,内有单层纤毛柱状上皮,可观察到肌层和管腔。 相似文献
12.
Rahul Sharma 《Marine Georesources & Geotechnology》2000,18(3):237-250
Observations on seafloor features from subbottom profiling and seabed photography data show that the undisturbed and natural conditions before the benthic disturbance undergo a substantial change after operation of the disturber. The effects can be seen in the form of deep trenches and grooves, formed by the action of the sleds and the pumping of the sediment along its path, as well as sediment piles on either side of the track. Biological traces such as fecal coils, casts, tubes, burrows, and trails get obliterated in the areas of disturbance and resedimentation. The smooth, uniform nature of the seafloor observed in predisturbance phase shows microtopographic changes in and around the disturbed area as a result of sediment excavation and resettlement. Vertical mixing as well as lateral transport of sediment alter the geological, biological, physical, and chemical conditions on the seafloor, which need to be monitored over time to assess the process and the time taken for restoration of environmental conditions. 相似文献
13.
Sediments of the continental slope are commonly bioturbated by endo- and epibenthic organisms, particularly in and around submarine canyons and channels. This study reviews the architecture and depositional environments associated with canyons and channels on the continental slope, and assesses the key physical and chemical conditions encountered in and around these conduits. Hydrodynamic energy, concentration and quality of organic carbon, dissolved oxygen concentration and sedimentation rate are identified as key controls on the composition of benthic ecosystems in slope environments. Submarine canyons and channels focus a variety of turbid and clear-water currents, all of which serve to increase the concentration of oxygen, labile organic carbon and other nutrients, which tend to elevate the abundance and biodiversity in the seafloor sediments, compared with those of the surrounding slope. Ancient slope channel and canyon systems reflect some of the variation in ichnological assemblages that is seen in modern analogues, although processes of erosion and trace fossil preservation mean that the benthic environment is often incompletely preserved in the ancient record. By integrating current understanding of sedimentology, oceanography, biology and ichnology of slope environments it is possible to provide a first order summary of the inter-relationships between ichnology and depositional environments on the continental slope. The combination of these data has the potential to improve our understanding of changes in deep marine benthic ecosystems through geological time, and to further the use of ichnology in assessing hydrocarbon reservoir presence, quality and performance from bioturbated slope, canyon and channel-levee hydrocarbon reservoirs. 相似文献
14.
Bacterial and cyanobacterial diversities determined by T-RFLP analyses in the Jiaozhou Bay 总被引：2，自引：0，他引：2
1Introduction Bacteriaareactivelyinvolvedinthedecomposi tionanddegradationoforganicmatterandxenobiotic compound,biogeochemicaltransformationsandnu trientrecyclingoflife essentialelements.Owingto freshwaterrunoff,wastewaterdischargeandaquacul turalpractice… 相似文献
15.
Xiaojun Xie Wu Tang Gongcheng Zhang Zhigang Zhao Shuang Song Shixiang Liu Yibo Wang Jia Guo 《海洋学报(英文版)》2023,42(3):123-137
This study involved outcrop, drilling, seismic, gravity, and magnetic data to systematically document the geological records of the subduction process of Proto-South China Sea (PSCS) and establish its evolution model. The results indicate that a series of arc-shaped ophiolite belts and calcalkaline magmatic rocks are developed in northern Borneo, both of which have the characteristics of gradually changing younger from west to east, and are direct signs of subduction and collision of PSCS. At the same time, the subduction of PSCS led to the formation of three accretion zones from the south to the north in Borneo, the Kuching belt, Sibu belt, and Miri belt. The sedimentary formation of northern Borneo is characterized by a three-layer structure, with the oceanic basement at the bottom, overlying the deep-sea flysch deposits of the Rajang–Crocker group, and the molasse sedimentary sequence that is dominated by river-delta and shallow marine facies at the top, recording the whole subduction–collision–orogeny process of PSCS. Further, seismic reflection and tomography also confirmed the subduction and collision of PSCS. Based on the geological records of the subduction and collision of PSCS, combined with the comprehensive analysis of segmented expansion and key tectonic events in the South China Sea, we establish the “gradual” subduction-collision evolution model of PSCS. During the late Eocene to middle Miocene, the Zengmu, Nansha, and Liyue–Palawan blocks were separated by West Baram Line and Balabac Fault, which collided with the Borneo block and Kagayan Ridge successively from the west to the east, forming several foreland basin systems, and PSCS subducted and closed from the west to the east. The subduction and extinction of PSCS controlled the oil and gas distribution pattern of southern South China Sea (SSCS) mainly in three aspects. First, the “gradual” closure process of PSCS led to the continuous development of many large deltas in SSCS. Second, the deltas formed during the subduction–collision of PSCS controlled the development of source rocks in the basins of SSCS. Macroscopically, the distribution and scale of deltas controlled the distribution and scale of source rocks, forming two types of source rocks, namely, coal measures and terrestrial marine facies. Microscopically, the difference of terrestrial higher plants carried by the delta controlled the proportion of macerals of source rocks. Third, the difference of source rocks mainly controlled the distribution pattern of oil and gas in SSCS. Meanwhile, the difference in the scale of source rocks mainly controlled the difference in the amount of oil and gas discoveries, resulting in a huge amount of oil and gas discoveries in the basin of SSCS. Meanwhile, the difference of macerals of source rocks mainly controlled the difference of oil and gas generation, forming the oil and gas distribution pattern of “nearshore oil and far-shore gas”. 相似文献
16.
红树林是具有重要碳汇功能的生态系统,其保护和修复成为海洋领域应对气候变化的重要手段。适宜的生境条件是红树林生态修复成功与否的关键因素,而红树植物物种和种植措施等也决定了修复后生态系统结构和功能的发展。本研究综述了红树林生态修复固碳功能的特点,分析了生境条件、物种和种植措施等因素对红树林生态系统固碳效果的影响,以期为红树林生态修复提供参考。总体上,红树林生态修复促进了有机碳在植物体和土壤中的累积,而在生态修复初期红树植被的固碳能力高于土壤。水文条件、滩涂高程、底质条件、物种、种植密度和种苗来源等关系到修复后红树植物的生长、颗粒态有机物的沉降、有机物的输出和保留、有机质的分解等过程,进而影响生态系统的固碳能力。因此,开展红树林生态修复时选择适宜生境条件的修复地块,合理选择物种和种植措施,在提高植被恢复效果的同时可提高红树林固碳增汇的生态功能。 相似文献
17.
目的：探讨人参醇提物及其沉淀对小鼠肠道菌群的影响。方法：利用环磷酰胺制备免疫低下小鼠模型,以人参水提物为对照,观察人参醇提物及其沉淀对小鼠肠道菌群的影响;肠道菌群数据采用Flash 1.2.11、Qiime 1.9.1、Mothur 1.30.2及PICRUSt 1.1.0等软件分析。结果：通过样本多样性指数分析,发现小鼠十二指肠、结肠、直肠所在部位肠道菌群结构差异具有统计学意义（P＜0.05或P＜0.01）。群落组成分析得知,小鼠肠道菌群差异较大的是Firmicutes(厚壁菌门),Bacteroides(拟杆菌门),Proteobacteria (变形菌门)与 Epsilonbacteraeota,Actinobacteria(放线菌门),Tenericutes(柔壁菌门)等。物种差异分析显示,免疫低下模型小鼠直肠部位的Epsilonbacteraeota数量显著增多(P=0.02),表明菌群失调;人参能调节肠道菌Epsilonbacteraeota失衡,其功效由大到小依次为人参水提物、醇提物及其沉淀。功能预测显示,肠道菌群结构改变,其对应的基因功能信息也改变,免疫低下模型小鼠直肠菌群结构与数量改变,引起RNA processing and modification, Chromatin structure and dynamics, Energy production and conversion 等方面基因改变。结论：免疫低下小鼠肠道菌群结构与功能改变,人参的不同提取物及其沉淀对小鼠肠道菌群有一定的影响。 相似文献
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海洋锋面存在于特征明显不同的2种或多种水系或水团交界处,锋面区域形成的次生环流和辐聚作用可显著影响到海洋中的物质输运与生物生产,故受到海洋学家的广泛关注。研究发现,我国近海陆架存在14个永久性的准静止锋面（渤海海峡锋面、山东半岛沿岸锋面、苏北沿岸锋面、西韩湾锋面、京畿湾锋面、济州岛西锋面、长江环形浅滩锋面、闽浙沿岸锋面、黑潮锋面、台湾沿岸锋面、闽粤沿岸锋面、珠江口沿岸锋面、琼东锋面和北部湾锋面）,且部分海域观测到双锋面、穿刺锋面和锋面波等现象。它们与陆架环流及其他动力过程（如：涡旋、内波等）共同控制着我国边缘海的物质能量输运与交换以及生物生产力格局。近岸物质沿锋面、跨锋面输运与锋区的垂向输送过程对我国边缘海生物地球化学循环和生态过程存在显著季节性影响。冬季到春季,沿岸锋面松弛能够加强物质从近岸向陆架的输运,进而在空间上调制春季藻华暴发的时间与量级;夏季到秋季,我国边缘海存在显著的潮汐锋面系统,锋面的辐聚效应以及次级环流可显著提高锋面区域的营养盐浓度和改善光照水平,对浮游植物的生长聚集起到促进作用,故在富营养化的河口与沿岸海域,锋面区域容易成为赤潮或缺氧高发区。此外,锋面的物理屏障作用使得两侧水团保持相对独立的物理与化学特征,因而在我国边缘海生境区划和生物多样性梯度变化等方面扮演重要角色,这些研究对认识我国边缘海物质循环与生物生产的控制机制具有重要作用。未来仍需充分结合观测与卫星资料,运用多过程耦合的高分辨率模型,深入认识锋面的精细结构与动态变化,加强亚中尺度和小尺度过程及其生态效应的研究。 相似文献
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为了评估北斗卫星导航系统（BDS）用于验潮站沉降监测的可靠性和精度,该文利用北海区沿海8个验潮站2022年全年的GPS和BDS观测数据,从基线解标准均方根误差、基线重复性和GPS与BDS基线互差等方面对GPS和BDS解算结果进行对比分析。结果表明,BDS基线解的NRMS值在0.17～0.25之间,GPS基线解的NRMS值在0.18～0.23之间,二者基线解的NRMS值基本相当;GPS基线解的长度和各分量基线重复率平均值要小于BDS,从GNSS网固定误差和比例误差看,GPS和BDS的基线长度重复性精度达到10-9～10-8量级,基线东西和南北分量重复性达到10-9量级,基线垂直分量重复性精度达到10-7～10-8量级。在GPS和BDS基线结果互差方面,GPS解算得到的基线长度均要大于BDS,二者平均差值为10.7 mm;东西分量差值最小,其次是南北分量,垂直分量差异最大,南北分量、东西分量和垂直分量差值平均值分别为-8.5 mm、-3.7 mm和10.6 mm。南北分量、东西分量、垂直分量和基线长度差值平均值最大值分别为23.6 mm、20.9 mm、30.4 mm和22.1 mm。总体... 相似文献