共查询到20条相似文献，搜索用时 531 毫秒
1.
2.
3.
WANG Lei MA Teng XU Wenteng CHEN Zhangfan ZHOU Qian ZHENG Guiliang CHEN Songlin 《中国海洋大学学报(英文版)》2021,20(2)
Interferon-induced protein with tetratricopeptide repeats 1(IFIT1), also known as interferon-induced protein 56(IFI56) or Interferon-stimulated protein 56(ISG56), was originally identified as a protein induced upon treatment with interferon and inhibited by viral replication and translational initiation. In this study, Epinephelus lanceolatus IFIT1(ELIFIT1) gene was cloned for the first time. The complete cDNA of El IFIT1 gene includes 2921 nucleotides, and encodes a 437-amino acid(AA) protein. The putative ELIFIT1 protein has 9 TRP domains and is highly similar with IFIT1 proteins in other teleosts. In healthy fish, ELIFIT1 gene was highly expressed in the blood, which indicate its specific function in the peripheral immune system. Its expression was also observed in various immunity-related tissues including spleen, intestine, and kidney, Inducted with spotted knifejaw iridovirus(SKIV), ELIFIT1 gene expression was upregulated in the spleen, kidney, and liver 24 h after induction and reached its peak at 72 h, indicating that ELIFIT1 may play an important role in antivirus. These findings contribute to the understanding of the antiviral regulation of ELIFIT1 gene in teleost. 相似文献
4.
5.
《中国海洋大学学报(英文版)》2016,(2)
Turbot(Scophthalmus maximus L.),a carnivorous fish species with high dietary protein requirement,was chosen to examine the expression pattern of peptide and amino acid transporter genes along its digestive tract which was divided into six segments including stomach,pyloric caeca,rectum,and three equal parts of the remainder of the intestine.The results showed that the expression of two peptide and eleven amino acid transporters genes exhibited distinct patterns.Peptide transporter 1(Pep T1) was rich in proximal intestine while peptide transporter 2(PepT2) was abundant in distal intestine.A number of neutral and cationic amino acid transporters expressed richly in whole intestine including B~0-type amino acid transporter 1(B~0AT1),L-type amino acid transporter 2(LAT2),T-type amino acid transporter 1(TAT1),proton-coupled amino acid transporter 1(PAT1),y~+L-type amino acid transporter 1(y~+LAT1),and cationic amino acid transporter 2(CAT2) while ASC amino acid transporter 2(ASCT2),sodium-coupled neutral amino acid transporter 2(SNAT2),and y~+L-type amino acid transporter 2(y~+LAT2) abundantly expressed in stomach.In addition,system b~(0,+) transporters(rBAT and b~(0,+)AT) existed richly in distal intestine.These findings comprehensively characterized the distribution of solute carrier family proteins,which revealed the relative importance of peptide and amino acid absorption through luminal membrane.Our findings are helpful to understand the mechanism of the utilization of dietary protein in fish with a short digestive tract. 相似文献
6.
采用多项式和结合周期项的混合函数模型进行GPS卫星钟差高精度模型化与精度分析。结果表明,周期项对于卫星钟差模型化精度的提高具有重要作用。对于Rb 钟卫星,Block ⅡF卫星钟差模型化精度0.03 m（约0.1 ns）左右,Block ⅡR和Block ⅡR-M卫星钟差模型化精度0.05 m（约0.2 ns）左右,而Cs钟卫星钟差模型化精度则低一个数量级。采用精密单点定位进行模型化结果分析得到,混合模型化钟差参与解算的定位结果精度可达cm级,收敛时间约为4 h。以上表明,简单的模型化参数可在一定程度上代替繁琐的序列钟差,实现简化GPS卫星钟差服务模式。 相似文献
7.
8.
Shuo Li Chunmei Li Xubo Wang Yanan Wang Zhipeng Liu Teng Zhai Quanqi Zhang 《中国海洋大学学报(英文版)》2010,9(1):59-64
A novel immune-related gene was expressed in Japanese flounder (Paralichthys olivaceus) injected with Vibrio anguillarum. The complete cDNA contained a 169 bp 5’UTR, a 336 bp open reading frame (ORF) encoding 111 amino acids and a 556bp 3’UTR.
Six exons and five introns were identified in the PoIR2 gene. Blastp similarity comparison showed its encoding protein had 50% similarity to Danio rerio neuromedin S (NMS), but further alignment indicated they did not have NMS C-terminal conservational signature domain. So
it was not defined as an NMS homologue. Protein structure analysis indicated it had a 26aa signal peptide and was a secretory
pathway protein. RT-PCR demonstrated that the expression of PoIR2 was quickly induced and drastically increased in liver, kidney, spleen, gills, intestine, heart, and skeletal muscle after
infected with V. anguillarum. These results indicated that the PoIR2 might play some important role in Japanese flounder immune response system. This gene was named PoIR2 (P.olivaceus immune-related gene 2, GenBank accession number: EU224372). The mature PoIR2 peptide was expressed in BL21(DE3) pLysS using pET-32a(+) vector and a great part of the recombinant mature peptide existed
as soluble type. 相似文献
9.
《中国海洋湖沼学报》2017,(4)
Although it has been hypothesized that a predictable feeding regime in animals allows physiological variables to be adjusted to maximize nutrient utilization and,hence,better growth performance,the assumption has rarely been tested.This study compares the effects of time-restricted versus free access self-feeding on the growth,feeding behavior and daily digestive enzyme rhythms of Atlantic salmon(Salmo salar).In an experiment that lasted 6 weeks,fish(109.9 g) were divided into two groups:group 1 had free access to a self-feeder(FA);group 2 received three meals per day(2 h per meal) at dawn,midday and dusk via a time-restricted self-feeder(TR).At the end of the experiment,the fish were sampled every 3 h over a24-h period.The results showed that the TR fish quickly synchronized their feeding behavior to the feeding window and their blood glucose showed a significant postprandial increase,while FA fish displayed no statistically significant rhythms(P0.05).Pepsin activity of TR fish also showed a significant daily rhythm(P0.05) with the acrophase at the second feed and a decrease over the next 12 h.Average daily trypsin,lipase and amylase levels of FA fish were significantly lower than those of TR fish(P0.01);however,the growth performance of both groups was similar(P0.05).In conclusion,the study failed to confirm a link between the entrainment of daily digestive enzyme profiles and growth performance,with the TR group showing comparatively poor blood glucose regulation. 相似文献
10.
为建立高精度的BDS钟差预报模型,提出一种基于改进的萤火虫算法优化的分数阶离散型灰色系统SAFA-FDGM(1,1)钟差预报模型。为避免萤火虫算法陷入局部最优解,提高萤火虫算法的优化能力,本文引入惯性权重因子,同时对吸引力因子、步长因子进行改进;利用改进的萤火虫算法自动优化选取FDGM(1,1)分数阶因子来提高FDGM(1,1)数据拟合精度。分别采用C02(GEO)、C09(IGSO)、C12(MEO)三种不同类型卫星的钟差数据进行实验分析,结果表明,本文预报模型优于传统二次多项式模型与GM(1,1)模型,其中3~6 h预报误差小于1 ns,9~12 h预报误差优于2 ns,对建立高精度的BDS卫星通用钟差预报模型具有重要参考价值。 相似文献
11.
Turbot (Scophthalmus maximus L.), a carnivorous fish species with high dietary protein requirement, was chosen to examine the expression pattern of peptide and amino acid transporter genes along its digestive tract which was divided into six segments including stomach, pyloric caeca, rectum, and three equal parts of the remainder of the intestine. The results showed that the expression of two peptide and eleven amino acid transporters genes exhibited distinct patterns. Peptide transporter 1 (PepT1) was rich in proximal intestine while peptide transporter 2 (PepT2) was abundant in distal intestine. A number of neutral and cationic amino acid transporters expressed richly in whole intestine including B0-type amino acid transporter 1 (B0AT1), L-type amino acid transporter 2 (LAT2), T-type amino acid transporter 1 (TAT1), proton-coupled amino acid transporter 1 (PAT1), y+L-type amino acid transporter 1 (y+LAT1), and cationic amino acid transporter 2 (CAT2) while ASC amino acid transporter 2 (ASCT2), sodium-coupled neutral amino acid transporter 2 (SNAT2), and y+L-type amino acid transporter 2 (y+LAT2) abundantly expressed in stomach. In addition, system b0,+ transporters (rBAT and b0,+AT) existed richly in distal intestine. These findings comprehensively characterized the distribution of solute carrier family proteins, which revealed the relative importance of peptide and amino acid absorption through luminal membrane. Our findings are helpful to understand the mechanism of the utilization of dietary protein in fish with a short digestive tract. 相似文献
12.
13.
以蓝圆鲹为原料,酶解蓝圆鲹鱼肉蛋白制备小分子肽。运用响应面试验设计优化蓝圆鲹蛋白酶解的工艺条件,探讨了酶种类和添加量、液固比、酶解温度、酶解时间对多肽提取率的影响。结果表明:选用木瓜蛋白酶,添加量为0.10%,液固比2∶1,酶解温度54.4℃,酶解时间3.18 h下,多肽提取率为32.35%。 相似文献
14.
基于15 d的精密卫星钟差数据,从不同角度全面分析6种常用钟差预报模型（LP模型、QP模型、GM模型、SA模型、ARIMA模型、KF模型）基于钟差一次差分预报原理的预报效果,得到以下结论：1）采用钟差一次差分预报原理,可以提高LP模型、SA模型、GM模型及KF模型对于GPS卫星钟差的3 h预报精度,提高QP模型和ARIMA模型对于ⅡF Rb钟的3 h预报精度,提高LP模型和GM模型在6 h和12 h预报中的精度,提高ARIMA模型在6 h、12 h和24 h预报中的精度;2）基于钟差一次差分预报原理的预报结果与卫星及其星载钟类型有关,对于GPS BLOCK ⅡF Rb钟,该预报原理可以提高6种模型的短期预报精度,特别是对GM模型、LP模型和ARIMA模型预报效果的改善最为显著;3）对于3 h和6 h的预报,采用钟差一次差分预报原理的LP模型（DLP模型）对应的RMS值都最小,即DLP模型的预报精度最高,说明钟差一次差分数据更适合一次多项式模型的短期预报。 相似文献
15.
提出一种基于EM算法优化相关向量机(RVM)的BDS-3超快速钟差预报算法。首先,利用组合MAD法预处理钟差数据,并进行一次差分计算;然后,利用钟差一次差分数据对RVM模型进行训练,通过EM算法迭代求取模型的超参数;最后,利用优化后的RVM模型进行数据预测,将钟差一次差分预测值还原,得到钟差预报值。采用iGMAS中心提供的实测BDS-3超快速钟差数据进行预报实验,并将本文模型与QP模型、SA模型及iGMAS超快速钟差预报产品(ISU-P)结果进行对比分析。结果表明,对于6 h、12 h和24 h预报,本文模型预报BDS-3卫星钟差数据的平均精度均优于0.61 ns;与ISU-P、QP模型和SA模型相比,本文模型预报24 h时精度分别提升64.1%、50.0%和49.2%。 相似文献
16.
???IGS??????IGU????????????????????????λ??PPP??????????????????????????IGS????????IGU??????????????????????????????PPP????????????????ж?λ?????????????????????????????????IGS?o??????????????????????0.1 ns???????????????PPP??????IGU??????PPP???????X??Y??Z???????????????87.1%??81.8%??91.3%??3D???????87.4%????????????????????????????? 相似文献
17.
《中国海洋大学学报(英文版)》2015,(6)
The complement system plays a crucial role in the innate immune system of animals. It can be activated by distinct yet overlapping classical, alternative and lectin pathways. In the alternative pathway, complement factor B(Bf) serves as the catalytic subunit of complement component 3(C3) convertase, which plays the central role among three activation pathways. In this study, the Bf gene in sea cucumber(Apostichopus japonicus), termed Aj Bf, was obtained by rapid amplification of c DNA ends(RACE). The full-length c DNA of Aj Bf was 3231 bp in length barring the poly(A) tail. It contained an open reading frame(ORF) of 2742 bp encoding 913 amino acids, a 105 bp 5'-UTR(5'-terminal untranslated region) and a 384 bp 3'-UTR. Aj Bf was a mosaic protein with six CCP(complement control protein) domains, a VWA(von Willebrand factor A) domain, and a serine protease domain. The deduced molecular weight of Aj Bf protein was 101 k Da. Quantitative real time PCR(q RT-PCR) analysis indicated that the expression level of Aj Bf in A. japonicus was obviously higher at larval stage than that at embryonic stage. Expression detection in different tissues showed that Aj Bf expressed higher in coelomocytes than in other four tissues. In addation, Aj Bf expression in different tissues was induced significantly after LPS or Poly I:C challenge. These results indicated that Aj Bf plays an important role in immune responses to pathogen infection. 相似文献
18.
基于iGMAS产品综合与服务中心（ISC）发布的ISC产品及德国波茨坦地学研究中心（GFZ）发布的GBM产品和GBM钟差重采样后的GBM-300 s产品对MGEX测站进行多系统的精密单点定位（PPP）测试,对2018-01-01～01-15全球15个MGEX测站的定位精度、收敛速度、ZTD求解精度和测站钟差求解精度进行分析。结果表明,在北斗PPP方面,使用ISC产品的3D定位精度和收敛速度优于GBM产品0.89 mm和 24 min,ZTD解算精度和测站钟差求解精度优于GBM产品0.3 mm和0.02 ns;在GPS、GLONASS、Galileo单系统PPP和四系统组合PPP方面,使用ISC产品的ZTD求解精度和测站钟差求解精度同GBM产品互差小于1.5 mm和0.05 ns,两者性能一致,没有明显差别,但使用ISC产品的PPP定位精度在高程方向低于GBM产品2~3 mm,收敛速度慢于GBM产品5~20 min。通过分析iGMAS产品的精度和服务的可靠性,为现阶段iGMAS产品性能的进一步提升给出建议。 相似文献
19.
《中国海洋湖沼学报》2021,(5)
Humpback grouper Cromileptes altivelis is one commercial fish with considerable economic value.To determine the expression stabilities of six commonly used internal reference genes in C.altivelis challenged by Vibrio harveyi and viral nervous necrosis virus(VNNV) through quantitative real-time PCR(qRT-PCR),the expression levels of selected genes in five immune organs stimulated with pathogenic infection were carefully evaluated using algorithms of geNorm,NormFinder,and BestKeeper.The results show that the expre ssion stabilities of the six candidate inte rnal reference genes were diffe re nt.Under no rmal physiological conditions,RPL13 were identified as the most stably expressed genes among five different immune organs(liver,spleen,kidney,intestine,and gill).After V.harveyi stimulation,RPL13,RPL13,EF1 A,RPL13,and EF1 A were identified by geNorm,NormFinder,and BestKeeper as the most stable genes in liver,spleen,kidney,intestine,and gill,respectively.Combining these three algorithms suggested that under stimulation of VNNV,RPL13,EF1 A,Actin,RPL13,and Actin were as the most stable genes in liver,spleen,kidney,intestine,and gill,respectively.These results suggest that specific experiment conditions and tissue types shall be considered when selecting the reference genes in qRT-PCR analysis.This study provided a solid foundation for future studies on gene expression of C.altivelis under different conditions. 相似文献
20.
Neuropeptide Y(NPY) is a 36-amino acid peptide of the neuropeptide Y family that plays key roles in the regulation of food intake. In this study,we focused on NPY m RNA expression changes around feeding time and during food deprivation in olive flounder. The olive flounder NPY m RNA levels were analyzed in different tissues and a high level of expression was detected in the brain. We also demonstrated a correlation between NPY expression levels in the brain and feeding schedule. NPY expression levels in olive flounder maintained on a daily scheduled feeding regimen increased shortly before feeding and decreased after the scheduled feeding time. Compared with the-1 h group before feeding,NPY expression in the 3 h group after feeding decreased significantly( P <0.05). Food deprivation led to an 81.7% decrease in NPY m RNA levels in the 24 h fasted group(P <0.05) and a 91.7% decrease in the 48 h fasted group(P <0.05). Therefore,our study demonstrates that NPY expression is associated with food intake in olive flounder. This result reveals the function of NPY in regulating food intake and its potential importance in olive flounder aquaculture. 相似文献