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1.
LIU Zhiliang HU Dunxin CHU Zhongxin 《中国海洋大学学报(英文版)》2008,7(1):17-26
Long term current observations in the southern Yellow Sea are very scarce because of the intense fishing and trawling activities. Most of the previous studies on tides and circulation were not rigorously validated with direct current measurements. In this study, tidal and sub-tidal currents were examined using current profiles from three bottom-moored Sontek Acoustic Doppler Profilers （ADPs） deployed in the southern Yellow Sea in the summers of 2001 and 2003. The measured current time series were dominated by tidal currents. The maximum velocities were between 40-80 cm s^-1 at the mooring stations. The M2 current was the dominant primary tidal constituent, while the MS4 and M4 components produced the most significant shallow water tidal currents with much weaker amplitudes. The measured mean sub-tidal velocities were less than 5 cmsl. The mean flows in the lower layer implied that an anti-cyclonic circulation pattern might exist in the deeper central Yellow Sea. However, the previously expected cyclonic circulation pattern in the upper layer was not clearly shown by the observations. 相似文献
2.
Identifying the structure of protected mountain ecosystems is an important task for understanding conservation sustainability. The study area, the Firtina Valley, located in the Rize City on the Eastern Blacksea Coast, is one of the biological hotspots and a National Park of Turkey. In order to identify the structure of mountain ecosystems, we generated a GIS database for the main environmental parameters of the study area, including elevation,slope and aspect layers for topographic structure, 10year mean values of Normalized Difference Vegetation Index(NDVI), data for vegetation structure, annual mean temperature and precipitation layers for climatic structure, main soil groups for soil structure and stream flow accumulation, stream flow length and stream order layers for hydrological structure.To identify the complex relations among environmental factors in the study area a data reduction method is applied with Principal Component Analysis(PCA). PCA is performed using data of 16 layers from Geographical Information Systems(GIS). PCA analysis reduced 16 dimensions into 5 dimensions containing 75% of the variation in all data. It is also revealed that the topographic structure, mainly altitude, dominates the ecosystems of the Firtina Valley, but it should be considered that the interactions of environmental factors in an ecosystem dynamics are very complex. The ecosystem structure is determined by the environmental factors direct or indirect effects on energy regulation of an ecosystem. Therefore the relationship between topographic elements and other abiotic-biotic elements in the Firtina Valley are important for environmental assessment and sustainability of a protected area, and these effects are explained in this study. 相似文献
3.
CTD data on standard levels coolected during July and December in 1998 and the cubic spline interpolating method were used to study the characteristics of the transition layer temperature and salinity.The thermocline undergoes remarkable seasonal variation in the South China Sea (SCS),and especially in the region of the north shelf where the thermocline disappears in december.The thermocline is stronger and thicker in July than in December,There is no obvious seasonal variation in the halocline.Due to the upper Ekman transport caused by monsoon over the SCS,the thermocline slopes upward in July and downward in december from east to west in the northern SCS.The characteristics of the thermocline and halocline are influenced by local eddies in the SCS.The Zhujiang diluted flow influences significantly the SCS shelf‘s halocline. 相似文献
4.
Dolmatova L.S. Eliseykina M.G. Timchenko N.F. Kovaleva A.L. Shitkova O.A. 《中国海洋湖沼学报》2003,21(4):293-304
Pure fraction (92% - 95%) of phagocytes (FP) and a mixture of amoebocytes (62%) and morula cells (38 % )-FPMC- of the holothurian Eupentacta fraudatrix ( Holothuroidea,Dendrochirota ) were obtained by using ficoll-verographine step gradient. Basal production of reactive oxygen species (ROS) in FP quantified by using reduction of nitroblue tetrazolium (NBT) was more than twice that in FPMC. Thermostable toxin of Yersinia pseudotuberculosis (TST) at differentconcentrations ( 0.2; 0.5; 2.5 μg/ml, but not 0.1 μg/ml) stimulated NBT reduction in FPMC after 24 h incubation. In FP, TST at concentrations of 0.1 and 0.2 μg/ml inhibited and at concentrations of 0.5 and 2.5 μg/ml stimulated NBT reduction after 24 h incubation. Maximal effect was observed in FP and FPMC at TST concentrations of 0.5 and 0.2 μg/ml, respectively. Addition of catalase (0.7 μg/ml) to the cells treated with TST (2.5 μg/ml) was followed by a decrease in NBT reduction compared to that under toxin treatment alone. TST stimulated superoxide dismutase activity in ccncentration-dependent manner (maximum at 0.5 μg/ml concentration in FP) after 24 htreatment, and this stimulation was prevented by a commercial catalase. Plant lectin concanavalin A stimulated NBT reduction more than 5-fold in FPMC compared to the control. With addition of TST, lectin stimulated ROS to lesser extent than that with lectin alone. When catalase, TST, and lectin were added into the FPMC simultaneously, ROS increase was similar to that under lectin treatment alone. On the whole, data obtained indicated that ROS generation in holothurian coelomocytes especially occurs in both stimulated and not stimulated phagocytes, and that changes in ROS production by these cells may be one of the mechanisms of antibacterial protection of holothurians. 相似文献
5.
LIU Qingzhi HU Yangdong 《中国海洋大学学报(英文版)》2008,7(1):43-47
Using molecular dynamics （MD） simulation, the diffusion of oxygen, methane, ammonia and carbon dioxide in water was simulated in the canonical NVT ensemble, and the diffusion coefficient was analyzed by the clustering method. By comparing to the conventional method （using the Einstein model） and the differentiation-interval variation method, we found that the results obtained by the clustering method used in this study are more close to the experimental values. This method proved to be more reasonable than the other two methods. 相似文献
6.
MENGXiangfeng WUDexing 《青岛海洋大学学报(英文版)》2002,1(2):119-124
Based on the analysis of Levitus data, the climatic states of the warm pool in the Indian Ocean (WPIO) and in the Pacific Ocean (WPPO) are studied. It is found that WPIO has a relatively smaller area, a shallower bottom and a slightly lower seawater temperature than those of WPPO. The horizontal area at different depths, volumes, central positions, and bottom depths of both WPIO and WPPO show quite apparent signals of seasonal variation. The maximum amplitude of WPIO surface area‘s seasonal variation is 58% larger over the annual mean value. WPIO‘s maximum volume variation amplitude is 66% larger over the annual mean value. The maximum variation amplitudes of the surface area and volume of WPPO are 20.9% and 20.6% larger over the annual mean value respectively. WPIO and WPPO show different temporal and spatial characteristics mainly due to the different wind fields and restriction of ocean basin geometry. For instance, seasonal northern displacement of WPIO is, to some extent, constrained by the basin of the Indian Ocean, while WPPO moves relatively freely in the longitudinal direction. The influence of WPIO and WPPO over the atmospheric motion must be quite different. 相似文献
7.
Contemporary villages of the mountain region are subject to uncontrolled structural and spatial transformations, which cause deformation of centuries-old spatial systems of high cultural and natural value. The aim of the study is to confront the opinions of the inhabitants and experts regarding the condition of the cultural landscape of the villages in south-eastern Poland. This area belongs to the Carpathian region of Central Europe. The historical and economic conditions of the studied region,... 相似文献
9.
Distribution, variation and impact factors of biomass of bacterioplankton from April to May 1999 in Bohai Sea were studied in DAPI method with epifluorescence microscopy. The biomass in surface waters showed a small day-night variation, varying from 0.13 to 2.51μg/dm^3 with an average of 0.84μg/dm^3. The biomass in bottom waters showed, however, a large variation, changing from 0.15 to 4.18μg/dm^3 with an average of 1.36μg/dm^3. The peak values occurred at 5 and 11 a.m. The bottom water biomass showed a significant correlation with particulate organic carbon （r=0.639, P〈0.05）. Heterotrophic bacterioplankton biomass was high in nearshore waters and low in offshore areas with a high biomass zone around Huanghe （Yellow） River mouth, showing the same distribution of nutrients. In vertical distribution, heterotrophic bacteria biomass in bottom waters was higher than that in surface water. 相似文献
10.
In the northern Songliao Basin, volcanics are fre-quently found and related closely to tectonic activi-ties during Early Cretaceous. The lava is mainly erupted along deep faults and distributed along the margine of the faulted depression. In deep part of Xujiaweizi area, natural gas exploration has been very successful, such as in the well Wang 9-12, well Shengshen 101 and well Fangshen 9 which are all highly-yield gas wells. Anda fault depression is lo-cated in the north of Xujia… 相似文献
11.
ＩＮＴＲＯＤＵＣＴＩＯＮＭｙｘｏｓｐｏｒｅａｎｓａｒｅｖｅｒｙｃｏｍｍｏｎｐａｒａｓｉｔｅｓｏｆｆｉｓｈ .Ｔｈｅｉｒｓｅａｓｏｎａｌｆｌｕｃｔｕａｔｉｏｎｓｉｎｐｒｅｖａｌｅｎｃｅａｎｄｉｎｔｅｎｓｉｔｙｏｆｉｎｆｅｃｔｉｏｎｗｅｒｅｄｅｓｃｒｉｂｅｄｂｙｓｏｍｅａｕｔｈｏｒｓ (Ｍｉｔｃｈｅｌｌ,1 989;Ｓｉｔｊａ ＢｏｂａｄｉｌｌａａｎｄＡｌｖａｒｅｚ Ｐｅｌｌｉｔｅｒｏ,1 990 ;Ｃｏｎｅ ,1 994 ) .Ｈｏｗｅｖｅｒ,ａｌｍｏｓｔｎｏｒｅｌａｔｅｄｉｎｆｏｒｍａｔｉｏｎｉｓａｖａｉｌａｂｌｅｉｎＣｈｉｎ… 相似文献
12.
A diplomonad flagellateHexamita capsularis sp. nov. from the posterior intestine of the fish (Xenocypris dividi) from Liangzi Lake, Hubei Province, is described. The flagellate was oval, with smooth surface and homogenous cytoplasm,
6.21±0.28 μm in length, 3.43±0.48 μm in width and with an elliptical capsule. Two recurrent flagellae arranged between two
nuclei ran longitudinally to the posterior end. Fibrillar bands connected the accessory organelles of two cytostomal tubes,
the body depressed at the posterior end of the body.
This work is a part of the M. S. thesis of Xiao Wu-han and was supported by the National Natural Science Foundation of China
(Grant No. 3870489). 相似文献
13.
ＩＮＴＲＯＤＵＣＴＩＯＮＴｈｅＪａｐａｎｅｓｅｓｅａｂａｓｓ (ＬａｔｅｏｌａｂｒａｘｊａｐｏｎｉｃｕｓＣ＆Ｖ)ｄｉｓｔｒｉｂｕｔｅｓｗｉｄｅｌｙｉｎｔｈｅｃｏａｓｔａｌｗａｔｅｒｓａｌｏｎｇｔｈｅＢｏｈａｉＳｅａ ,ＹｅｌｌｏｗＳｅａ ,ＥａｓｔＣｈｉｎａＳｅａ,ａｎｄＳｏｕｔｈＣｈｉｎａＳｅａ .Ａｓａｃａｒｎｉｖｏｒｏｕｓｆｉｓｈ ,ｉｔｏｃｃｕｐｉｅｓｔｈｅｈｉｇｈｔｒｏｐｈｉｃｌｅｖｅｌｉｎｔｈｅｍａｒｉｎｅｅｃｏｓｙｓｔｅｍａｎｄｈａｓａｄａｐｔｅｄｔｏｖａｒｉｏｕｓｅｎｖｉｒｏｎｍｅｎｔａｌｒ… 相似文献
14.
Bacterin was prepared by formalin-inactivating the virulent strain of Vibrio hollisae isolated from diseased Seriola dumerili （amberjack） suffering from vibriosis. Healthy S. dumeriIi were vaccinated by respective procedures of intramuscular injection, immersion, and orally administration. Results of the three different vaccinations were compared. Blood was drawn from the vaccinated fish every 7 days, and the antibody titers and lysozyme activities of the sera were determined. The antibody titer of injected fish was 1：40 at 7 d, and reached its peak of 1：320 at 28 d, while the fish vaccinated by immersion and orally administration exhibited weak antibody responses, the antibody titres of 〈1：10, 1：20, 1：160 were observed at 7 d, 14 d, 35 d respectively. Compared with the control, the vaccinated fish exhibited significantly higher lysozyme activities （P〈0.05）. Upon challenge with virulent strain, the relative percent survival （RPS） of injected, immersed and oral administrated fish were 75%, 45%, and 40% respectively, and the injected fish showed significantly higher RPS than immersed and oral administrated fish. The results suggested that vaccination of S. dumerili by the injection would be the best strategy to prevent the vibriosis in S. dumerili farm. 相似文献
15.
For the investigation of anti-infection immune response of Pseudosciaena crocea, 160 healthy fish samples were categorized into infected and control groups. Each individual fish in the infected group was
injected intraperitoneally (i.p.) with 0.2 ml bacterial suspension of Vibrio alginolyticus in density of 2×107 CFU/ml, while each individual in the control group was injected i.p. with 0.2 ml sterile saline solution (0.85%). It was
observed that the artificial injection of V. alginolyticus significantly increased the number of erythrocytes, leucocytes, lymphocytes in peripheral blood as well as peripheral serum
antibacterial activity and antibody titer of large yellow croaker, and significantly reduced the number of peripheral blood
granulocytes as compared with those in the control group. No significant difference in acid phosphytase and superoxide dismutase
activity of serum was detected between the two groups. It is suggested that non-specific immune factors including leucocytes
and anti-bacteria substance in peripheral blood played important role at the initial stage of infection, and specific immune
factors such as antibody then played important role in response to anti-infection at the latter stage.
Supported by the National High Technology Research and Development Program of China (863 program, No. 2007AA09Z115) and Technology
Program of Xiamen (No. 3502Z73019) 相似文献
16.
The ultrastructure of hemocytes ofPenaeus vannamei was examined by transmission electron microscope. Three types of hemocytes were identified: hyaline cell, small-granule cell
and large-granule cell. Hyaline cells are the smallest of the hemocytes, lack electron-dense granules, have average size of
11.11 μm (SE±0.50,n=10)×6.96μm (SE±0.55,n=10), comprise 25.8% (SE±2.87,n=4×10) of the hemocytes; small granule cells are the most abundant type of hemocytes, comprise 58.1% (SE±3.40,n=4×10) of the hemocytes, contain small electron—dense granules, have average size of 10.78 μm (SE±0.60,n=10)×8.63 μm (SE±0.44,n=10); and large-granule cells comprise 16.1% (SE±2.55,n=4×10) of the hemocytes, are filled with large electron dense granules, have average size of 12.51 μm (SE±0.63,n=10)×8.99μm (SE±0.71,n=10).
This research was supported by the National Climbing Project B, PDB6-6-3.
Contribution No. 3448 from the Institute of Oceanology, Chinese Academy of Sciences. 相似文献
17.
The marine chroococooid phycoerythrin-containingSynechococcus spp. cyanobacterium has been implicated as a substantial component of the photosynthetic picoplankton in the ocean. Although
its importance as food source for heterotrophic nanoplankton is now recognized, information about the cycling ofSynechococcus biomass and its diel pattern is limited and study methodology varies among authors. The selective metabolic inhibitor method
was used to simultaneously estimate growth and grazing disappearance rates ofSynechococcus in the English Channel where growth rates ranged from 0.25 to 0.72/d (mean ±SD=0.51±0.17/d) and grazing mortality rates ranged
from 0.19 to 0.64/d (mean ±SD=0.48±0.17/d). Size-fractionated experiments demonstrated that up to 70% ofSynechococcus disappearance could be attributed to grazers going through a 2 μm Nuclepore filter.Synechococcus grazing mortality rates (mean=0.74 ±0.25/d) during the day were always higher than that (mean=0.2±0.20/d) during the night,
while growth rates showed no clear diel pattern. A positive correlation was observed between growth rates andin situ temperature ranging from 9 to 17°C, while in contrast grazing was independent of temperature. The close similatiry between
average growth and grazing rates suggests a rapid recycling ofSynechococcus biomass in English Channel coastal waters. 相似文献
18.
A VIBRIO ANGUILIARUM STRAIN ASSOCIATED WITH SKIN ULCER ON CULTURED FLOUNDER, PARALICHTHYS OLIVACEUS 总被引：2，自引：2，他引：0
The characteristics of a bacterium strain M3, isolated from cultured flounderParalichthys olivaceus with remarkable external sign of skin ulcer during an epizootic outbreak, indicated that the bacterium belonged to the speciesVibrio anguillarum. Challenge by I.M. (intramuscular injection), bath, and oral administration with M3 showed that it was highly pathogenic
forParalichthys olivacues. The LD50 dose was 5.144×103 CFU/ per fish infection by I.M. injection. Recovered inoculated bacteria from the surviving fish revealed that the asymptomatic
carriers could be a latent contagious source. Study of the effect of bacterial culture CFS (cell-free-supernatant) showed
that the exotoxins produced by M3 play an important role in its pathogenicity for flounder. The resistance of M3 to 36 out
of 41 antibiotics indicated that the bacterial disease outbreak was mainly attributable to the frequent and excessive use
of antimicrobial agents; and that vaccination would be an effective precaution against bacterial disease.
Contribution No. 4107 from Institute of Oceanology, Chinese Academy of Science.
Contribution No. 270 from Experimental marine Biology Laboratory, CAS.
Funded by projects under the Major State Basic Research Development Program, Grant G1999012003. 相似文献
19.
Serum immunoglobulin of the mandarin fish, Siniperca chuatsi with development of polyclonal antibody
Serum immunoglobulin from the mandarin fish, or the so-called Chinese perch,Siniperca chuatsi (Basilewsky), was successfully purified using affinity chromatography. Heavy and light chains were detected on electrophoresis
gel, with molecular weights being estimated at 72 and 29 kDa, respectively. The tetrameric IgM ofS. chuatsi was calculated to be 808 kDa. The rabbit polyclonal antisera against the purifed immunoglobulin were developed and tested
by Western blot analysis. The antisera reacted strongly with the heavy chains ofS. chuatsi immunoglobulin. Humoral immune responses of the mandarin fish can then be examined using the developed polyclonal antibody.
Project No. 30025035 supported by NSFC, State Key Laboratory of Freshwater Ecology and Biotechnology (2000FB02), and the Chinese
Academy of Sciences (KZ9511-B1-111). 相似文献
20.
The effect of dietary phosphorus on the growth and body components of juvenile Synechogobius hasta was determined. Different percentages of dietary phosphorus (0.63, 0.77, 0.93, 1.06, 1.22 and 1.36) were tested by feeding
the fish (body weight, 15.81 g ± 0.32 g; 20 individuals each group; 3 groups each percentage) at a surplus of 5%–10% above
satiation for 35 d. Dietary phosphorus did not significantly affect the specific growth rate, feed intake, feed conversion
ratio and protein efficiency rate. Nitrogen retention was found to be the highest in fish fed the diet containing 1.06% of
phosphorus; however, this was not significantly different from other diets. Fish fed the diet containing 0.93% of phosphorus
showed the highest phosphorus retention; similar phosphorus retention rates were found in fish fed the diets containing 0.77%
and 1.06% of phosphorus. Fish fed the diet containing the highest percentage of dietary phosphorus were found to contain the
least whole body lipid, lower than fish fed other diets (P<0.05). The protein content increased from 18.59% to 19.55% (although not significant) with the decrease of body lipid content
(P>0.05). The contents of the whole body ash, whole body phosphorus and vertebrae phosphorus increased with dietary phosphorus
percentage up to 1.06 (P<0.05), reaching a plateau after that. Dietary phosphorus did not significantly influence the muscle components (protein,
lipid and moisture). Condition factor and hepatosomatic index were the highest in fish fed the diet containing 0.63% of dietary
phosphorus; however, this was not significantly different from those of other diets. The second-order polynomial regression
of phosphorus retention against dietary phosphorus identified a breakpoint at 0.88% of dietary phosphorus. However, the dietary
requirement of phosphorus for maintaining maximum phosphorus storage determined by broken-line analysis of the contents of
whole body phosphorus, and ash and vertebrae phosphorus was 1.06% of the diet. 相似文献