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1.
In this study, the entire mitochondrial DNA （mtDNA） control region （CR） of Pholis fangi was amplified via polymerase chain reaction followed by direct sequencing. The length of the mtDNA CR consensus sequence of P. fangi was 853 bp in length. In accordance with the recognition sites as were previously reported in fish species, the mtDNA CR sequence of P. fangi can be divided into 3 domains, i.e., the extended terminal associated sequence （ETAS）, the central conserved sequence block （CSB）, and the CSB domain. In addition, the following structures were identified in the mtDNA CR sequence of P. fangi：2 ETASs in the ETAS domain （TAS and cTAS）, 6 CSBs in the central CSB domain （CSB-F to CSB-A）, and 3 CSBs in the CSB domain （CSB-1 to CSB-3）. These demonstrated that the structure of the mtDNA CR of P. fangi was substantially different from those of most other fish species. The mtDNA CR sequence of P. fangi contained one conserved region from 656 bp to 815 bp. Similar to most other fish species, P. fangi has no tandem repeat sequences in its mtDNA CR sequence. Phylogenetic analysis based on the complete mtDNA CR sequences showed that there were no genetic differences within P. fangi populations of the same geographical origin and between P. fangi populations of different geographical origins. 相似文献
2.
Reversible protein phosphorylation, catalyzed by protein kinases and phosphatases, is an important and versatile mechanism by which eukaryotic cells regulate almost all the signaling processes. Protein phosphatase 1 （PP1） is the first and well-characterized member of the protein serine/threonine phosphatase family. In the present study, a full-length cDNA encoding the beta isoform of the catalytic subunit of protein phosphatase l（PPlcb）, was for the first time isolated and sequenced from the skin tissue of flatfish turbot Scophthalmus maximus, designated SmPPlcb, by the rapid amplification of cDNA ends （RACE） technique. The cDNA sequence of SmPPlcb we obtained contains a 984 bp open reading frame （ORF）, flanked by a complete 39 bp 5＇ untranslated region and 462 bp 3＇ untranslated region. The ORF encodes a putative 327 amino acid protein, and the N-terminal section of this protein is highly acidic, Met-Ala-Glu-Gly-Glu-Leu-Asp-Val-Asp, a common feature for PP1 catalytic subunit but absent in protein phosphatase 2B （PP2B）. And its calculated molecular mass is 37 193 Da and pI 5.8. Sequence analysis indicated that, SmPPlcb is extremely conserved in both amino acid and nucleotide acid levels compared with the PPlcb of other vertebrates and invertebrates, and its Kozak motif contained in the 5＇UTR around ATG start codon is GXXAXXGXXATGG, which is different from mammalian in two positions A6 and G3, indicating the possibility of different initiation of translation in turbot, and also the 3＇UTR of SmPPlcb is highly diverse in the sequence similarity and length compared with other animals, especially zebraf＇lsh. The cloning and sequencing of SmPPlcb gene lays a good foundation for the future work on the biological functions of PP1 in the flatfish turbot. 相似文献
3.
MAHongming XUWei MAIKangsen LIUFUZhiguo CHENHong 《中国海洋大学学报(英文版)》2004,3(2):145-149
1 Introduction Actinisanimportantcontractileproteinineukary oticcells .Itisoneofthetwomajorcomponentsin volvedinthecontractionofmusclecells .Innon mus clecells ,itisthemajorpartofcytoskeletoninvolvedinmanyprocessessuchascellmotility ,endocytosis ,exocyt… 相似文献
4.
5.
Optimal salinity for dominant copepods in the East China Sea, determined using a yield density model
From 1997 to 2000, four field surveys were conducted in the East China Sea (ECS) (23°30′–33°00′N, 118°30′–128°00′E). A field
data yield density model was used to determine the optimal salinities for 19 dominant copepod species to establish the relationship
between surface salinities and abundance of those species. In addition, ecological groups of the copepods were classified
based on optimal salinity and geographical distribution. The results indicate that the yield density model is suitable for
determining the relationship between salinity and abundance. Cosmocalanus darwini, Euchaeta rimana, Pleuromamma gracilis, Rhincalanus cornutus, Scolecithrix danae and Pareucalanus attenuatus were determined as oceanic species, with optimal salinities of >34.0. They were stenohaline and mainly distributed in waters
influenced by the Kuroshio or Taiwan warm current. Temora discaudata, T. stylifera and Canthocalanus pauper were nearshore species with optimal salinities of <33.0 and most abundant in coastal waters. The remaining 10 species, including
Undinula vulgaris and Subeucalanus subcrassus, were offshore species, with optimal salinity ranging from 33.0–34.0. They were widely distributed in nearshore, offshore
and oceanic waters but mainly in the mixed water of the ECS. 相似文献
6.
1 INTRODUCTION The unicellular Dunaliella salina, is one of the well studied microalgae for mass culture and is of commercial importance as a source of natural beta-carotene (Avron and Ben-Amot, 1992). D. sa- lina has desirable properties of halotolerance… 相似文献
7.
Three new spades and a new variety of nannoplankton, Chrysochromulina papillata, Gaysochromulina chiton var. minuta, Paraphysomonas simplexocorbita and Paraphysomonas bisorbulina are reported in this paper. All were isolated from the preliminary culture samples of seawater collected from Jiaozhou Bay, Shandong, China. The three species occurred at Station 1(120° 14.56′ E, 36°4′N) in November 1984, the new variety at Station 2 (120° 16.35° E,36°4.5′N) in January, 1985. The morphological features, especially the structures of the scales of these new nannoplankton,. are described. The differences between the new species and the related ones are discussed; their movement and nutrition, and the temperature and salinity of their biotopes are also mentioned. 相似文献
8.
In this report, complete mitochondrial genome sequences of Laminaria cultivation variety ‘Rongfu’ were obtained. The results showed the length of circular molecule of mtDNA was 37 638 bp (64.7%
A+T), encoding three rRNAs (23S, 16S and 5S), 25 tRNAs, 35 known mitochondrial proteins and 3 ORFs. Sequence alignment indicated
its mtDNA genome was very similar to that of Laminaria japonica. Phylogenetic trees inferred from concatenated 30 mitochondrial genes showed that ‘Rongfu’, Laminaria japonica, Laminaria longipedalis, Laminaria diabolica, Laminaria religiosa and Laminaria ochotensis clustered together. In addition, compared with mitochondrial genome of L. japonica, ‘Rongfu’ mtDNA lacked a non-coding region of 19 nucleotides, which was located between rRNA small subunit gene 3 (rps3) and rRNA small subunit gene 9 (rps9). Seven cultivation varieties of China were divided into two groups based on this non-coding region which was absent in
‘Rongfu’, ‘Fujian’ and ‘Sanhai’ while present in ‘Ailunwan’, ‘Dongfang No.2’, ‘Dongfang No.3’ and ‘Zaohoucheng’. So this variation
can be used in germplasm identification of cultivation variety. 相似文献
9.
3-bromo-4,5-bis(2,3-dibromo-4,5-dihydroxybenzyl)-1,2-benzenediol (1) is a natural bromophenol isolated from the red algae Rhodomela confervoides that exhibits significant inhibition against protein tyrosine phosphatase 1B (PTP1B). Based on its activity, we synthesized two new synthetic bromophenols and their methoxy derivatives from vanillin using the structure of natural bromophenol 1 as a scaffold. The structures of these bromophenols were elucidated from 1H NMR, 13C NMR, and high resolution electron ionization mass spectrometry as 2,3-dibromo-1-(2p-bromo-6p-(3q,4q-dimethoxybenzyl)- 3p,4p-dimethoxybenzyl)-4,5-dimethoxybenzene(2),2,3-dibromo-1-(2p-bromo-6p-(2q-bromo-4q,5q-dimethoxy-benzyl)-3p,4p-dimethoxybenzyl)-4,5-dimethoxybenzene(3),3,4-dibromo-5-(2p-bromo-6p-(2q-bromo-4q,5q-dihydroxybenzyl)-3p,4p-dihydroxybenzyl)pyrocatechol(4)and 3,4-dibromo-5-(2p-bromo-6p-(3q,4q-dihydroxybenzyl)-3p,4p-dihydroxybenzyl)pyrocatechol (5).PTP1B inhibition activities of these compounds were evaluated using a colorimetric assay,and compounds 3 and 4 demonstrated interesting activity against PTP1B. 相似文献
10.
A complementary DNA (cDNA) library was constructed from half-smooth tongue sole spleen. A long-read expressed sequence tag
(EST) database was generated, containing 3100 cDNA clones, of which 220 clones were fully sequenced. A total of 1060 non-redundant
simple sequence repeats (SSRs) were obtained from the cDNA library. An average of 5 kb sequence generates 1 SSR in the half-smooth
tongue sole spleen cDNA library. The proportion of the SSR unit size was different in the cDNA library. The monomeric repeats
(51.4%) are the most abundant class of SSR in the dataset. The dimeric, trimeric, tetrameric and hexameric repeats are represented
in decreasing proportions of 27.2%, 16.0%, 2.8% and 1.9%, respectively. The frequency of pentameric repeats was observed the
least (only 0.7%). Most of the monomeric and dimeric repeats are distributed in 3′ and 5′ un-translation region. If translation
regions are considered merely, trimeric repeats are the highest, accounting for 57% of the total microsatellites. 相似文献
11.
邢福武，李泽贤，叶华谷，陈炳辉，吴德邻ＡＳＴＵＤＹＯＮＴＨＥＦＬＯＲＩＳＴＩＣＰＬＡＮＴＧＥＯＧＲＡＰＨＹＯＦＸＩＳＨＡＩＳＬＡＮＤＳ，ＳＯＵＴＨＣＨＩＮＡ￥ＸｉｎｇＦｕｗｕ；ＬｉＺｅｘｉａｎ；ＹｅＨｕａｇｕ；ＣｈｅｎＢｉｎｇｈｕｉ；ＷｕＤｅｌｉｎ（... 相似文献
12.
Bromophenols are a set of natural products widely distributed in seaweed, most of which exhibit interesting and useful biological
activities. To develop a reliable and efficient synthetic route to these natural bromophenols, three of them, 3,4-dibromo-5-(2′-bromo-3′,4′-dihydroxy-6′-methoxymethyl-benzyl)-benzene-1,2-diol
(compound 9), 3,4-dibromo-5-(2′-bromo-6′-ethoxy methyl-3′,4′-dihydroxybenzyl)-benzene-1,2-diol (compound 10), and 3-bromo-4-(3′-bromo-4′,5′-dihydroxy benzyl)-5-(ethoxymethyl)-benzene-1,2-diol (compound 14), isolated from red marine algae, have been synthesized in eight steps with an overall yield of 14.4%, 14.4%, and 18.2% respectively,
via a practical approach employing bromination, Wolff-Kishner-Huang reduction and a Friedel-Crafts reaction as key steps.
The protein tyrosine phosphatase 1B (PTP1B) inhibitory activities of the synthetic compounds were evaluated by the colorimetric
assay. The results show that these compounds are moderate PTP1B inhibitors. The synthesis of these bromophenol derivatives
makes in vivo studies of their structure-activity relationships and inhibition activity against PTP1B possible. 相似文献
13.
Two new species of the asteroid family Ophidiasteridae are described from two specimens collected by commercial fishery vessels
of Qingdao from the vicinity of Diaoyudao (25°45′N, 123°15′E), East China Sea.Hacelia tuberculata sp. nov. shows affinities withH. tyloplax (H. L. Clark, 1914) andLinckia gracilis sp. nov. is related toL. laevigata (Linnaeus).
Contribution No. 1163 from the Institute of Oceanology, Academia Sinica. 相似文献
14.
Xinping Zhang 《中国地理科学(英文版)》1997,7(4):339-346
The relations between δ18O and temperature on the different time scales were analysed, according to the data from Tuotuohe (34°13′N, 96°25′E; 4533
m a. s. l.), Delingha (37°22′N, 97°22′E; 2981 m a. s. l.) and Xining (36°37′N, 101°46′E; 2261 m a. s. l.) in the Qinghai-Xizang
Plateau. The results show that the significance of d δ18O/dT on different time scales are different. The d δ18O/dT on the synoptic scale reflects the interdependent relation between δ18O and temperature in the short-term synoptic scale process; the d δ18O/d T on the seasonal scale reflects the relation between them whithin a year; and the d δ18O/d T on the climatic scale reflects the relation between them in the long-term climatic change. The calculated d δ18O/dT on climatic scale is very close to the theoretical values on the condition of advection transport for Tuotuohe Station. However,
there are great differences between the calculated and the theoretical values for Delingha and Xining stations. 相似文献
15.
Twenty-four compounds including eight steroids (1–8), nine triterpenoids (9–16, 24), three flavonoids (20–22), and four benzenecarboxylic derivatives (17–19, 23) were isolated and identified from stems and twigs of medicinal mangrove plant Sonneratia caseolaris. The structures of the isolated compounds were determined by extensive analysis of their spectroscopic data. Among these
metabolites, compounds 1, 4–20 and 22–24 were isolated and identified for the first time from S. caseolaris. In the in vitro cytotoxic assay against SMMC-7721 human hepatoma cells, compound 21 (3′,4′,5,7-tetrahydroxyflavone) exhibited significant activity with IC50 2.8 μg/mL, while oleanolic acid (14), 3,3′-di-O-methyl ether ellagic acid (18), and 3,3′,4-O-tri-O-methyl ether ellagic acid (19) showed weak activity. None of these compounds displayed significant antibacterial activites.
Supported by the National Natural Science Foundation of China (No. 30770234); Knowledge Innovation Program of Chinese Academy
of Sciences (KZCX2-YW-211-04); Department of Science and Technology of Shandong Province (No.2006GG2205023) 相似文献
16.
Effects of alginate gel at different concentrations on rheological properties of hair-tail (Trichiurus lepturus) surimi were investigated. Alginate gel (1% - 3%) exhibited enhanced effects, especially when alginate gel concentration increased. The rheological properties of mixture samples were studied by the time sweep, frequency sweep and temperature sweep. The critical strain values of the mixture samples for the onset of non-linear viscoelasticity were about 5%. The storage modulus G’ of the mixture samples increased with time for 4 h. The frequency sweep showed that G’ was greater than G" for all the mixture gels with different alginate gel concentrations, and values of both n’ and n" for all samples were low (<0.2), these constants corresponding to G’ and G", and indicating the elasticity of mixture gels. The values of storage modulus G’ decreased during heating process and increased with decreasing temperature. 相似文献
17.
To investigate the antitumor effect of bromophenol derivatives in vitro and Leathesia nana extract in vivo, six bromophenol derivatives 6-(2,3-dibromo-4,5-dihydroxybenzyl)-2,3-dibromo-4,5-dihydroxy benzyl methyl ether (1), (+)-3-(2,3-dibromo-4,5-dihydroxyphenyl)-4-bromo-5,6-dihydroxy-1,3-dihydroisobenzofuran (2), 3-bromo-4-(2,3-dibromo-4,5-dihydroxybenzyl)-5-methoxymethyl-pyrocatechol (3), 2,2′,3,3′-tetrabromo-4,4′,5,5′-tetrahydroxy-diphenylmethane (4), bis(2,3-dibromo-4,5-dihydroxybenzyl) ether (5), 2,2′,3-tribromo-3′,4,4′,5-tetrahydroxy-6′-ethyloxymethyldiphenylmethane (6) were isolated from brown alga Leathesia nana, and their cytotoxicity were tested by MTT assays in human cancer cell lines A549, BGC-823, MCF-7, B16-BL6, HT-1080, A2780,
Bel7402 and HCT-8. Their inhibitory activity against protein tyrosine kinase (PTK) with over-expression of c-kit was analyzed also by ELISA. The antitumor activity of ethanolic extraction of Leathesia nana (EELN) was evaluated on S180-bearing mice. All compounds showed very potent cytotoxicity against all of the eight cancer cell lines with IC50 below 10 μg/mL. In PTK inhibition study, all bromophenol derivatives showed moderate inhibitory activity and compounds 2,
5 and 6 showed significant bioactivity with the inhibition ratio of 77.5%, 80.1% and 71.4%, respectively. Pharmacological
studies reveal that EELN could inhibit the growth of Sarcoma 180 tumor and increase the indices of thymus and spleen to improve
the immune system remarkably in vivo. Results indicated that the bromophenol derivatives and EELN can be used as potent antitumor agents for PTK over-expression
of c-kit and considered in a new therapeutic strategy for treatment of cancer.
Supported by the National High Technology Research and Development Program of China (863 Program, No. 2007AA09Z410) and Knowledge
Innovation Program of Chinese Academy of Sciences (No. KZCX2-YW-209) 相似文献
18.
Study on the dominant species of Pteropoda in East China Sea 总被引：2，自引：0，他引：2
1 INTRODUCTION Pteropoda is a group of marine pelagic mol- lusks, which belongs to Opisthobranchia of Gastro- poda. Although Pteropoda is not a major group in pelagic zooplankton in terms of abundance and number of species, their ecological characters are… 相似文献
19.
The fatty acid compositions of seston and Calanus sinicus were investigated to study trophic relationships in Jiaozhou Bay. Principal component analysis was carried out to ordinate the fatty acid patterns of seston in stations and months. The results showed that diatoms were most abundant in the phytoplankton at station A5 (located in the northwest of the bay:36°9′N, 120°20′E) and least abundant at station D7 (located outside of the bay: 35°59′N, 120°26′E). By contrast, dinoflagellates were most abundant at station D7 and least abundant at station A5. According to the annual variations of 16:1ω7 and 18:4ω3/ 16:1ω7, diatoms flourished mainly in spring and summer, while dinoflagellates bloomed exclusively in summer. A distinctive feature of the fatty acid composition of C. sinicus was the prevalence of 20:5ω3 and 22:6ω3. The higher content of 16:1ω7 over 18:4ω3 in females indicated that diatoms contributed more than dinoflagellates to the diet of C. sinicus. The feeding intensity of C. sinicus on diatoms was higher in spring and autumn than in other seasons. The herbivorous indicators 20:1 and 22:1 were comparatively low, suggesting that besides phytoplankton, C. sinicus might feed on a wider range of particles including organic detritus, bacteria and small copepods. 相似文献
20.
A putative tetrasporophyte-specific gene, designated as SSH466 (GenBank accession No. DQ019223), was one of the genes identified
in this work using suppression subtractive hybridization (SSH) method in Gracilaria lemaneiformis. The full length of the gene was obtained using SMART RACE strategy. Sequence analysis revealed that the gene had 1 019 nucleotides,
including an open reading frame of 498 nucleotides encoding 166 amino acid residues, 158 nucleotides of 5′ untranslated region
and 363 nucleotides of 3′ non-coding region. Protein motif and secondary structure prediction showed that there existed a
transmembrane domain with a unique β-sheet. Thus, SSH466 protein might be a cross-membrane protein. Sequence homology search
in the public GenBank databases did not reveal any significant match with SSH466. Virtual Northern blot analysis confirmed
that it was a tetrasporophyte-specific gene. 相似文献