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1.
从北极海洋环境中筛选分离出一株产胞外碱性蛋白酶的革兰氏阴性杆菌 Ar/ W/ b/ 75°2 5′ N/ 1。该菌能在 7～ 30℃范围内生长 ,并且在 30℃条件下生长良好 ,但不能在 40℃条件下生长。该菌在 0 .5%～ 1 0 % Na Cl的盐浓度范围内能良好生长 ,表现出一定的嗜盐性。葡萄糖、蔗糖及可溶性淀粉等碳源物质可被该菌株利用以供生长之需 ,其中以葡萄糖最佳。氮源物质中 ,蛋白胨是最有利于菌株生长、产酶的有机氮源 ,而 NH4 NO3则是效果最好的无机氮源。对该菌蛋白酶的酶学特性研究表明 :所产胞外蛋白酶占其蛋白酶总量的 75.7%左右 ;蛋白酶反应的最适温度为40℃ ,酶活力在温度不高于 40℃条件下保持稳定 ;该菌蛋白酶具有较宽的 p H活性范围 ,在 p H7～ 1 1均表现出较高酶活力 ,其最适 p H在 8左右 相似文献
2.
Extracellular enzymatic activities of cold-adapted bacteria from polar oceans and effect of temperature and salinity on cell growth 
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下载免费PDF全文 The potential of 324 bacteria isolated from different habitats in polar oceans to produce a variety of extracellular enzymatic activities at low temperature was investigated. By plate assay, lipase, protease, amylase, gelatinase, agarase, chitinase or cellulase were detected. Lipases were generally present by bacteria living in polar oceans. Protease-producing bacteria held the second highest proportion in culturable isolates. Strains producing amylase kept a relative stable proportion of around 30% in different polar marine habitats. All 50 Arctic sea-ice bacteria producing proteases were cold-adapted strains, however, only 20% were psychrophilic. 98% of them could grow at 3% NaCl, and 56% could grow without NaCl. On the other hand, 98% of these sea-ice bacteria produced extracellular proteases with optimum temperature at or higher than 35℃, well above the upper temperature limit of cell growth. Extracellular enzymes including amylase, agarase, cellulase and lipase released by bacteria from seawater or sediment in polar oceans, most expressed maximum activities between 25 and 35℃. Among extracellular enzymes released by bacterial strain BSw20308, protease expressed maximum activity at 40℃, higher than 35℃ of polysaccharide hydrolases and 25℃ of lipase. 相似文献
3.
Isolation and characteristics of one marine psychrotrophic cellulase-generating bacterium Ar/w/b/75°/10/5 from Chuckchi Sea,Arctic 下载免费PDF全文
下载免费PDF全文 Microorganisms living in polar zones play an important part as the potential source of organic activity materials with low temperature characteristics in the bio-technological applications. A psychrotrophic bacterium (strain Ar/w/b/75°/10/5) , producing cellulose at low temperatures during late-exponential and early-stationary phases of cell growth, was isolated from sea ice-covered surface water in Chuckchi Sea, Arctic. This bacterium, with rod cells, was Gram-negative, slightly halophilic. Colony growing on agar plate was in black. Optimum growth temperature was 15℃. No cell growth was observed at 351 or above. Optimum salt concentration for cell growth was between 2 and 3 % of sodium chloride in media. Maximal cellulase activity was detected at a temperature of 35℃ and pH8. Cellulase was irreversibly inactivated when incubated at 55℃ within 30 min. Enzyme can be kept stable at the temperature no higher than 25℃. Of special interest was that this bacterium produced various extracellular enzymes i 相似文献
4.
Screening and molecular classification of low-temperature protease from Antarctic microorganism and its characterization 下载免费PDF全文
下载免费PDF全文 <正> 107 strains producing protease were screened from 260 strains of Ant-arctic psychrophilic bacteria,among which proteolytic activity of five strains wasmore than 45 U ml~(-1).The 16S rRNA gene sequences homology and phylogenet-ic analysis of five Antarctic psychrophilic bacteria showed that NJ276、NJ5-9、NJ16-70、NJ345 belonged to the described genus Pseudoalteromonas and NJ341belonged to the genus Colwellia.The growth and the protease characteristic offour Antarctic psychrophilic bacteria had been studied,and the result showedthat the optimal temperature for growth and protease-producing of four strainswas about 10℃.Their growth and protease-producing were still high during in-cubating 2-5 days.The maximum proteolytic activity occurred at pH 9 for fourAntarctic psychrophilic bacteria.The optimal temperature of protease action ofboth strains NJ276 and NJ5-9 was about 50℃,however,the optimal temperatureof protease action of both strains NJ341 and NJ345 was about 40℃,and theirproteolytic activity under 0℃ exhibited nearly 30% of the maximum activity,but their thermal stabilities were weaker.These results indicated that proteasesfrom NJ341 and NJ345 were low-temperature proteases. 相似文献
5.
从北极楚科奇海水中分离到一株产蛋白酶耐冷河豚毒素假交替单胞菌BSw20353。该菌可合成多种胞外蛋白酶,而培养温度影响蛋白酶的合成。分离纯化出其中一种在15℃、25℃培养时均有生成的酶:该酶最适作用温度55℃;最适pH8;金属离子Hg2+、Sr2+抑制酶活,Fe2+、Mn2+则具有激活作用;该酶属于金属蛋白酶,受螯合剂邻二氮杂菲、EDTA及EGTA抑制,基本不受丝氨酸蛋白酶抑制剂PMSF、TPCK及TLCK抑制,半胱氨酸蛋白酶抑制剂E-64对该酶无抑制作用,天冬氨酸蛋白酶抑制剂pepstatin则具有一定抑制作用;SDS、二硫苏糖醇、碘乙酸可抑制酶活;而在变性剂尿素、盐酸胍作用下,酶活反而有所增高。 相似文献
6.
Primary productivity and chlorophyll a in Prydz Bay and its mouth in Antarctica during the austral summer of 1999/2000 下载免费PDF全文
下载免费PDF全文 The investigation of phytoplankton standing stock, euphotic layer and photosynthesis rate were carried out in 19 27 of January, 2000 at three longitudinal sections (70°30′E, 73°00′E and 75°30′E). The results showed that the high value of chlorophyll a concentration was in inshore bay, polynya and the continental slope of the investigated sea area. At various investigated stations, average chlorophyll a concentration at sub surface layer (25 m) was higher than that at surface layer; its concentration at the deeper layers of over 50 m decreased with increasing depth. At anchor station, the maximum chlorophyll a concentration appeared at surface layer in Antarctic summer's afternoon while the minimum value appeared in the morning; chlorophyll a concentration at water layer of 0 25 m was obviously higher than that at deep water layer, being related to the releasing of ice algae. High productivity was in inshore bay and polynya of continental shelf. Chlorophyll a concentration at surface layer is closely correlated to the dissolved oxygen concentration in seawater. The sea area with chlorophyll a concentration of over 1.0 μg/dm 3 may be the convergence of CO 2 and that of below 1.0 μg/dm 3 may be the source of CO 2. 相似文献
7.
Effect of isolation temperature on the characteristics of extracellular proteases produced by Antarctic bacteria 总被引：1，自引：0，他引：1
Protease-producing psychrotolerant bacteria were isolated from Antarctic biotopes on casein agar plates using different incubation temperatures. Most of the isolates were non-spore-forming Gram-negative motile rods with catalase activity, 30% were pigmented and none of them were glucose fermenters. All the strains were grown in liquid cultures at 20°C and protease secretion was tested using the azocasein method. Despite their capacity for production of a clear zone of hydrolysis in agar plates, some strains did not produce detectable levels of proteolytic activity in liquid cultures. The lowest apparent optimum temperature for protease activity found in culture supernatants was 40°C. Almost all the strains showed activation energy values about 10-20 kJ-mol−1 lower than that observed for a mesophilic Subtilisin. Most of the proteases showed optimal activity at neutral or alkaline pH values and developed a multiple-band profile on gelatine-SDS-PAGE. It was observed that the lower the strain isolation temperature was, the more stongly cold-adapted–in terms of optimal temperature and activation energy–were the proteases produced by them. This dependence of the characteristics of the proteases on the isolation temperature is an important factor to take into account in the design of screening programmes directed towards the isolation of psychrotolerant bacteria able to produce proteases strongly or weakly adapted to work in the cold. The Antarctic area explored proved to be a promising source of proteolytic bacteria with potential use in industrial processes to be carried out at low or moderate temperatures. 相似文献
8.
红球菌(Rhodococcus sp.)B7740分离自北冰洋海水样品,前期实验表明其能产生大量黄色素,但是该菌生物量很低。本实验通过碳源、氮源、培养温度、发酵液起始pH值、菌种接种量等单因素和正交实验优化了该菌株的培养条件,在此基础上,通过对提取剂种类及pH、提取时间、料液比等的研究,优化了色素的提取条件。实验结果表明,1L陈海水中,添加5g酵母粉,5g葡萄糖,3g乳糖,7g牛肉膏;起始pH7,培养温度25℃,菌种接种量1.0%,160r/min摇床培养7d时,生物量从6.1mg/mL上升到23.08mg/mL,提高了278.4%。以上述培养条件下得到的干菌体为材料,采用pH7的无水乙醇,当料液比1:30,浸提温度75℃,浸提1h重复提取2次时,色素含量为1160.625μg/g干菌体。对色素的分析表明,该色素为类胡萝卜素,实验结果为进一步研究极地微生物红球菌(Rhodococcus sp.)B7740产类胡萝卜素的提取工艺提供了基础。 相似文献
9.
Studies on culture condition and extracellular hydrolase of psychrophilic bacteria from Arctic sea ice 下载免费PDF全文
下载免费PDF全文 Arctic sea ice in the polar region provides a cold habitat for microbial community.Arctic sea ice microorganisms are revealed to be of considerable impor- tance in basic research and potential in biotechnological application.This paper in- vestigated the culture condition and extracellular hydrolase of 14 strains of different Arctic sea ice bacteria.The results showed that optimal growth temperature of strains is 15℃or 20℃.The optimal pH is about 8.0.They hardly grow at acid condition. 3% NaCl is necessary for better growth.These strains have different abilities in pro- ducing amylase,protcase,cellulase and lipase.Pseudoalteronomas sp.Bsi429 and Pseudoalteronomas sp.Bsi539 produced both cellulose,protease and lipase.These results provide a basis for further developing and exploiting the cold adapted marine enzyme resources. 相似文献
10.
Sebastian H. Mernild Bent Hasholt Bjarne H. Jakobsen Birger U. Hansen 《Geografisk tidskrift / udgivet af Bestyrelsen for Det Kongelige danske geografiske selskab》2013,113(1):153-161
Geografisk Tidsskrift, Danish Journal of Geography 108(1):153–161, 2008 Meteorological stations have been in operation since 1993 at the Sermilik Research Station (65°40′N, 38°10′W), Ammassalik Island, Southeast Greenland. This note presents meteorological observations for the calendar year 2006 from the two meteorological stations: Station Nunatak (515 m a.s.l.) and Station Coast (25 m a.s.l.). The year 2006 record is not complete due to a temporal break down of Station Coast (from end-of June through beginning of August). Special focus is given to ground temperature measurements at the meteorological Station Nunatak (1994–2006) 0.1 and 0.3 m below the surface. Data are presented to illustrate the intra- and inter-annual temperature variability and possible trends in the upper part of the soil. 相似文献
11.
覆盖在北极海洋上的广阔海冰为其内部生存的微生物群落提供了一个独特的生境。研究表明 ,大量海冰细菌能够分泌产生胞外酶 ,其中产蛋白或脂质水解酶细菌的比例远高于产多糖水解酶的细菌。温度、盐度是直接影响海冰细菌生存与活力的 2个主要环境因子。 76%的产酪蛋白酶海冰细菌为低温菌 ,菌株只能在 <35°C条件下生长 ;而 98%的酪蛋白酶最适作用温度≥ 35°C ,其中 62 %的酶最适作用温度≥ 45°C。几乎所有的产酪蛋白酶海冰细菌都耐盐或嗜盐 相似文献
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13.
The fabrics and crystals of Bohai one year ice show that the noncontinuous ice growth rate enables the level ice layers with different amount of air bubbles to be formed in lower part of an ice sheet which was clearly seen from CT technology; typical grain ice and columnar ice occur in the grey ice which grows in stable water; thaw refrozen ice and rafted ice have their specific crystal characters. On the Arctic sea ice, the ice core located at 72°24.037′N, 153°33.994′W and 2.2 m in length was a 3 year ice floe and a new sort of crystal was found, which is defined as refrozen clastic pieces. The crystal profile of the ice core 4.86 m in length located at 74°58.614′N, 160°31.830′W shows the evidence that ice ridge changed into hummock. 相似文献
14.
从中国第二次北极科学考察采集的海冰样品中分离到8种细菌,研究了盐度对其生长的影响。结果表明,8株海冰细菌对盐度有着广泛的适应性,在盐度为30-90的范围内均可较快生长,其中菌株BJ2、BJ3和BJ7的盐耐受性较强。进一步的研究表明,BJ2、BJ3和BJ7在0-150 的盐度范围内均有不同程度的生长,盐度为0-30时,菌株的生长最快;盐度为180时,这三株菌株也均有短暂的生长。随着盐度的增加,三株菌株生长的延迟期增加,到达生长稳定期的时间也相应增加。温度对菌株BJ2、BJ3和BJ7的盐耐受性也有着一定的影响,随着温度的升高, 菌株的生长速度变慢,但盐耐受性有所增加。 相似文献
15.
试验选用一株高产低温蛋白酶的南极细菌菌株Pseudoalteromonas sp. AN64,从溶解氧、化学需氧量、氨氮、亚硝态氮、pH值5个方面研究了南极细菌对养殖水体的影响。结果显示,随着饲料中蛋白质的散失,水体中蛋白质的含量在第7天达到高峰,溶解氧下降,化学需氧量持续上升,在第11天达最大值9.88 mg/L,并且维持在较高的水平。缺氧条件下,有害物质氨氮和亚硝态氮含量逐渐增加,在第9天和第11天分别达最大值0.65 mg/L和0.352 mg/L,而pH值持续下降,在第9天达最低值5.95。当水体中加入高产蛋白酶的南极菌株AN64后,蛋白质含量在第5天即达高峰,溶解氧无明显变化,化学需氧量在第7天达最高峰,比对照组提前4天,但含量仅约为后者的一半。同时,氨氮和亚硝态氮含量远低于对照组,分别仅为对照的47.7 %和26.5 %,而pH值有小幅下降,维持在6.85到7.52之间。由此可以看出,南极菌株产生的低温蛋白酶可以有效分解海水中的蛋白质,降低水体中的氨氮和亚硝态氮等有害物质,维持pH值的稳定性,为健康养殖提供了有效途径。 相似文献
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本文报道了 1 999年夏季白令海中部浮游动物的分布特征。初步鉴定共有 90种浮游动物 ,它们分别隶属于北极、亚北极、大洋冷水和世界广温等四种生态类群 ,但以亚北极类群为主导 ,尤以太平洋亚北极外海广深种布氏真哲水蚤、羽哲水蚤、晶额哲水蚤和太平洋腹水蚤等占优势 ,呈现太平洋亚北极外海浮游动物的群落特征。在水平方向上 ,浮游动物总生物量 (均值为42 5 .4mg/m3)介于 70— 1 1 30mg/m3之间 ,呈现南侧高于北侧 ,周边高于中央的平面分布格局。在垂直方向上 ,5 0 0m以浅水域浮游动物总生物量日均值以 5 0— 0m层最高 ( 4 3% ) ,2 0 0— 1 0 0m层最低 ( 8.4% )。此外 ,在 1 0 0m以浅水层中浮游动物总生物量的垂直分布具显著的昼夜变化 ,高值层呈现白天下降、夜晚上升的分布节律 ,不同种群浮游动物分布的水层不尽相同 ,甚至同一种群不同发育阶段的个体分布的水层亦不同。 相似文献
17.
根据 1 999年 7月至 8月我国首次北极科学考察期间“雪龙”号考察船在楚科奇海 ( 66°0 .3′N75°1 8.6′N ,1 5 3°36.5′W 1 74°5 9.5′W )和白令海 ( 5 5°5 9.8′N66°0 .3′N ,1 73°2 1 .1′E1 75°5 3.9′W)采集的 5 1份网采样品和 2 4份水采样品 ,鉴定浮游植物 3个门类 38属 1 2 1种 (含变种和变型 ) ,都是真核藻类。其中楚科奇海有 33属 1 0 3种 ,白令海有 2 5属 71种 ,两海区共有种 49种。这些种类可分为 4个生态类群 :( 1 )北极类群 ;( 2 )北极、亚北极北方类群 ;( 3)北方温带类群 ;( 4 )世界性广温类群等。主要优势种有楚科奇海的格鲁菱形藻 (Nitzschiagrunowii)、诺登海链藻(Thalassiosiranordenskioldi)和聚生角毛藻 (Chaetocerossocialis)等和白令海的西氏细齿状藻(Denticulaseminae)、柔弱菱形藻 (Nitzschiadilicatissima)、成列菱形藻 (N .seriata)和长海毛藻(Thalassiothrixlongissima)等。楚克奇海浮游植物的平均丰度 ( 8.32× 1 0 7个 /m3)远高于白令海( 1 .5 8× 1 0 6个 /m3)。文中还讨论了调查区浮游植物的分布特点及其与环境的关系。 相似文献
18.
During August 1999, we investigated sea ice characteristics; its distribution, surface feature, thickness, ice floe movement, and the temperature field around inter-borders of air/ice/seawater in the Chukchi Sea. Thirteen ice cores were drilled at 11 floe stations in the area of 72°24′ 77°18′N, 153°34′ 163°28′W and the ice core structure was observed. From field observation, three melting processes of ice were observed; surface layer melting, surface and bottom layers melting, and all of ice melting. The observation of temperature fields around sea ice floes showed that the bottom melting under the ice floes were important process. As ice floes and open water areas were alternately distributed in summer Arctic Ocean; the water under ice was colder than the open water by 0.4 2.8℃. The sun radiation heated seawater in open sea areas so that the warmer water went to the bottom when the ice floes move to those areas. This causes ice melting to start at the bottom of the ice floes. This process can balance effectively the temperature fluctuating in the sea in summer. From the crystalline structure of sea ice observed from the cores, it was concluded that the ice was composed of ice crystals and brine-ice films. During the sea ice melting, the brine-ice films between ice crystals melted firstly; then the ice crystals were encircled by brine films; the sea ice became the mixture of ice and liquid brine. At the end of melting, the ice crystals would be separated each other, the bond between ice crystals weakens and this leads to the collapse of the ice sheet. 相似文献
19.
Germination responses of Medicago ruthenica seeds to salinity,alkalinity, and temperature 总被引：2，自引：0，他引：2
To determine the effects of and the interactions between salinity and temperature, and alkalinity and temperature, we conducted three germination tests of Medicago ruthenica (L.) Sojak cv. ‘Zhilixing’ seeds in growth chambers. In experiment 1, the seeds were placed at a range of temperatures (5, 10, 15, 20, 25, 30, 35, or 40 °C), and in experiments 2 and 3, we varied the salinity (0, 50, 100, 150, 200, or 250 mM NaCl) and alkalinity (1, 5, 10, 15, 20, or 25 mM Na2CO3), respectively, within three ranges of alternating temperatures (10–20, 15–25, and 20–30 °C). The seeds of M. ruthenica (L.) Sojak cv. ‘Zhilixing’ showed high percentage germination at all temperatures, except for 5 °C. Seeds germinated well at low NaCl and Na2CO3 concentrations under all three alternating temperature regimes. Approximately half the seeds germinated at high salinity and alkalinity at 15–25 °C, whereas the ungerminated seeds germinated when the high-saline and alkaline stresses were removed. These results suggest that M. ruthenica (L.) Sojak cv. ‘Zhilixing’ has potential utility as a forage legume in saline and alkaline environments. 相似文献