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1.
Exposure to environmentally relevant concentrations of different nonylphenol formulations in Japanese medaka. 总被引：1，自引：0，他引：1
The time course of exposure to p-nonylphenol (NP) from two different sources was compared to equivalent exposures of 17-beta-estradiol (E2) and a solvent control (ethanol; EtOH). Japanese medaka were exposed for 4 days to a nominal concentration of 20 micrograms/l of either NP-I (Schenectady International, Inc.), NP-II (Aldrich), or E2, and were then placed in untreated water for 5 days. Tissue samples were taken at two time points during the 4-day exposure and two time points during the 5 days following exposure. Liver homogenates were analyzed using a western blot to detect vitellogenin (VTG) and quantified by measuring the optical density for each lane. Preliminary results indicate that E2 significantly increased VTG staining above the level observed in EtOH-treated controls for both males and females. A two-way analysis of variance (ANOVA) indicates that NP from both sources, as well as E2, significantly increased VTG staining in males (ANOVA, n = 48, P < 0.001; Tukey pairwise tests, all P < 0.008). A significant increase in VTG was observed in E2-treated males and females the first day following transfer into toxicant-free water (two-way ANOVAs, both n = 48, P < 0.003; Tukey pairwise tests, all P < 0.019). If confirmed, this extended response observed for low-level exposures may represent a significant factor for sampling scenarios following pulsitile exposure. 相似文献
2.
In this study, we exposed female medaka to aqueous solutions of o,p′-DDT until tissues residues reached approximately 100 g/g. Male offspring of exposed females and unexposed males that were raised to 6 months of age differed from untreated controls in their response to subsequent 4-day static exposure to 17-β-estradiol (E2; 12 μg/l as determined by steroid EIA). Homogenized liver samples from males from DDT treatments, females from DDT treatments, control males and control females produced two vitellogenin bands on a Western blot with no qualitative differences in band size and location between the groups. To quantify the relative level of vitellogenin, 5 μg of protein from each sample was analyzed by slot blot. A statistical comparison revealed a significant difference in optical density of the slots between control males and DDT exposed males (analysis of variance, n=18, P=0.021; Tukey test, n=9, P=0.047) indicating that previous transgenerational exposure to DDT enhanced the vitellogenic response to E2 exposure in males. These results indicate that exposure to estrogenic chemicals may produce an ‘estrogen memory’ which potentiates the response to a subsequent exposure event. 相似文献
3.
Rauschenberger RH Wiebe JJ Buckland JE Smith JT Sepúlveda MS Gross TS 《Marine environmental research》2004,58(2-5):851-856
Alligator mississippiensis eggs from organochlorine pesticide (OCP) contaminated sites in Florida exhibit high rates of embryonic mortality compared to reference sites (P < 0.05). The objective of the present study was to use captive adult alligators to test the hypotheses that maternal exposure to OCPs results in increased OCP concentrations in eggs, and that increased exposure is associated with increased embryonic mortality. A total of 24 adult alligators (8 males and 16 females) were housed in eight pens. Eight females in four pens were dosed with a mixture of p,p'-DDE, toxaphene, dieldrin, and chlordane at a rate of 0.2+/-0.01 mg/kg/day for 274+/-8 days. Treated females produced eggs containing higher OCP concentrations (12,814+/-813 ng/g yolk) than controls (38+/-4 ng/g yolk). Eggs of treated females exhibited decreased viability (13+/-22%) as compared to controls (45+/-20%). Results indicated that 0.6% of administered OCPs were maternally transferred to the eggs of American alligators, and that maternal exposure is associated with decreased egg/embryo viability in this species. 相似文献
4.
Studies were conducted to characterize effects of a known androgen on sexual differentiation and development of medaka, Oryzias latipes (d-rR strain), at two life stages. Embryos were injected with graded doses of methyl testosterone (MT) prior to epiboly. The occurrence of sex-reversal, and the gonadosomatic index (GSI) were evaluated in adults. Primary germ cells were counted and gonad volumes calculated for larvae to determine if sex-reversal could be detected at an early life stage. Sex-reversal of genetic females to phenotypic males was observed at both life stages. The GSI for phenotypic females was greater than for phenotypic males, while the GSI in XX males was similar to XY males. MT appeared to reduce the GSI of XX females exposed to MT but not sex-reversed. Our results indicate that embryonic exposure to androgens influences sexual development in medaka. Utilizing the d-rR strain of medaka allows detection of an effect as early as 2 weeks after chemical exposure making this a useful tool to screen chemicals for effects on sexual differentiation. 相似文献
5.
The catchability of western rock lobsters (Panulirus cygnus) during the spawning season (October and November) may be affected by their reproductive state. In this study, movement and food consumption of males and females of different reproductive states held in laboratory tanks were measured as proxies for catchability. Density of lobsters was found to influence both movement and food consumption. Solitary animals and, to a lesser extent, two lobsters per tank, were more sedentary and consumed significantly less food than animals at a density of three or four specimens per tank. Tests using three animals per tank confirmed that mature, unmated, and ovigerous females carrying early stage eggs moved more frequently away from their shelters than males or females carrying late stage eggs (P < 0.0001, d.f. = 3). Further, unmated females and females with early stage eggs recorded significantly higher food consumption (P < 0.0061, d.f. = 3) compared with males or females carrying late stage eggs. The greater time spent away from shelter and also food consumption make unmated females and females with early stage eggs likely to be more catchable than males or females with late stage eggs. As catchability is commonly used to obtain population estimates from survey data, these results have implications for surveys which use catch rates of breeding animals as indicators of egg production. 相似文献
6.
2,4-二氯苯氧乙酸对细基江蓠孢子生长和存活的影响 总被引：1，自引：0，他引：1
以细基江蓠(Gracilaria tenuistipitata)的果孢子和四分孢子为实验材料,用不同浓度的(0,0.1,0.5,1,2,4,6 mg/L)生长激素2,4-二氯苯氧乙酸(2,4-D)处理刚刚放散的孢子和附着2d孢子,研究其对果孢子和四分孢子附着和生长及存活的影响。与对照组相比,2,4-D使两种孢子的附着量明显降低;2.0~4.0mg/L的2,4-D可以极显著的促进孢子的生长(P<0.01);0.1~2.0mg/L的2,4-D提高孢子的存活率(P<0.01);6.0mg/L的2,4-D使得孢子的生长和存活率降低。综合以上指标,在细基江篱采孢子育苗时,孢子附着2~3d后用2,4-D处理孢子萌发体,以1.0~2.0mg/L为宜。 相似文献
7.
Abstract. Routine monthly samples of the commercially important portunid crab Liocarcimtx puber and the sympatric but ecologically separated L. holsatus were collected from the waters and shores around the Gowcr Peninsula, South Wales, between November 1983 and September 1985.
The pubertal moult in L. puber occurred at 38 mm CW (carapace width) (females) and 42 mm CW (males). In L. holsatus this occurred at 17 mm (females) and 18.5 mm CW (males). The median size of ovigcrous females was 49.2 ± 6.7 mm CW in L. puber and 26.5 ± 2.0 mm CW in L. holsatus.
Moulting and copulation occurred between spring and autumn, the moulting period of males being earlier than that of the females. Ovigcrous L. holsatus were recorded throughout the year, with highest proportions between February and April. L. puber were ovigcrous mainly between January and March; none was recorded between September and November. The number of eggs of the wild brood (range: 39,000–280,000 for L. holsatus and 40.000–262.000 for L. puber ) was related to female body size as y = 3.099.51 c0.1126x , r = 0.90, n = 21 and y = 6,335.98 c0.051x , r = 0.88, n = 23, respectively, where y = number of eggs and x = carapace width (mm).
Following successful copulation, females of both species were able to spawn more than once during an intcrmoult period. 相似文献
The pubertal moult in L. puber occurred at 38 mm CW (carapace width) (females) and 42 mm CW (males). In L. holsatus this occurred at 17 mm (females) and 18.5 mm CW (males). The median size of ovigcrous females was 49.2 ± 6.7 mm CW in L. puber and 26.5 ± 2.0 mm CW in L. holsatus.
Moulting and copulation occurred between spring and autumn, the moulting period of males being earlier than that of the females. Ovigcrous L. holsatus were recorded throughout the year, with highest proportions between February and April. L. puber were ovigcrous mainly between January and March; none was recorded between September and November. The number of eggs of the wild brood (range: 39,000–280,000 for L. holsatus and 40.000–262.000 for L. puber ) was related to female body size as y = 3.099.51 c
Following successful copulation, females of both species were able to spawn more than once during an intcrmoult period. 相似文献
8.
9.
We exposed sexually maturing male rainbow trout (Oncorhynchus mykiss) to BDE-47 (a polybrominated diphenyl ether) and female rainbow trout to trenbolone (an anabolic steroid). Male trout were orally exposed for 17 days to 55 microg/kg/day BDE-47 and female trout continuously exposed for 60-77 days to a measured trenbolone water concentration of 35 ng/L. After the exposure, eggs and semen were collected and in vitro fertilization trials performed using a sperm:egg ratio of 300,000:1. In the BDE-47 study, eggs from control females were fertilized with semen from exposed males, while in the trenbolone study, eggs from exposed females were fertilized with semen from control males. All treatments were evaluated at two-three early developmental time-points representing first cleavage (0.5 day), embryonic keel (9 days), and eyed stages (19 days), respectively. The results indicated that BDE-47 exposure did not alter fertility as embryonic survival was similar between control and exposed groups. Trenbolone exposure also did not alter embryo survival. However, in the embryos fertilized with eggs from trenbolone exposed females, a noticeable delay in developmental progress was observed. On day 19 when eye development is normally complete, the majority of the embryos either lacked eyes or displayed under-developed eyes, in contrast to control embryos. This finding suggests steroidal androgen exposure in sexually maturing female rainbow trout can impact developmental timing of F1 offspring. 相似文献
10.
Leaños-Castañeda O Gold-Bouchot G Van Der Kraak G Lister A Ceja-Moreno V Simá-Alvarez R 《Marine environmental research》2004,58(2-5):337-342
The objective of this study was to determine if tamoxifen or an aromatase inhibitor (4-hydroxyandrostenedione; 4-OHA) affected plasma concentrations of o,p' -DDT and its metabolites, o,p'-DDD and o,p'-DDE, in mature tilapia. Male and female tilapia were injected 6 times intraperitoneally with o,p'-DDT (40 mg/kg) alone or in combination with 4-OHA (2 mg/kg) over a 12 day period. An additional group of male fish was injected with tamoxifen (5 mg/kg) plus o,p'-DDT. At the end of the treatment period, plasma samples were extracted and analyzed by GC/ECD. Females injected only with o,p'-DDT had significantly higher levels of o,p'-DDT compared with males. Interestingly, females and males treated concomitantly with o,p'-DDT and 4-OHA or tamoxifen had significantly lower concentrations of plasma o,p'-DDT (about 50%) compared with fish treated with only o,p'-DDT. These initial results suggest that an interaction between endocrine-active compounds occurs in vivo in tilapia and may involve alterations in metabolism of o,p'-DDT. 相似文献
11.
W. R. Hartley A. Thiyagarajah M. B. Anderson M. W. Broxson S. E. Major S. I. Zell 《Marine environmental research》1998,46(1-5)
The purpose of this research was to evaluate the Japanese medaka (Oryzias latipes) as an ecotoxicological model for the rapid evaluation of environmental estrogens. A novel short-term (48-h) exposure to 17 β-estradiol is proposed in development of a positive control for disruption of gonadal development. Recently hatched medaka fry (30 fry per dose group) with undifferentiated gonads were exposed to 4.0, 29.4, and 115.6 μg/liter of 17 β-estradiol (acetone carrier) for 48 h in a water bath at 25 °C. The fry were then grown-out in spring water for 2 weeks, killed, and processed for histological evaluation. High lethality was encountered during the grow-out period in the 115.6 μg/liter dose group. Fry in the spring water and acetone (carrier) control groups developed into females or males. Fry exposed to 17 β-estradiol developed primarily into females or had testis-ova. 相似文献
12.
Scott AP Sanders M Stentiford GD Reese RA Katsiadaki I 《Marine environmental research》2007,64(2):128-148
Dab (Limanda limanda) caught in UK offshore waters show evidence of being exposed to estrogenic endocrine disrupters at a relatively low level. Two of 449 males caught between June and July 2005 had markedly elevated levels of vitellogenin (VTG; 21 and 750 microg/ml) and the remainder ranged from <0.01 to 8.6 microg/ml. Omitting the two outliers, there was a very significant positive relationship with the mass of individual males (a feature noted in previous studies on cod). Mean VTG concentrations in males differed significantly between sites. The site with the highest mean (1.6 microg/ml) was North East of the Dogger Bank and the site with the lowest (0.04 microg/ml) was in Cardigan Bay. Mean VTG concentrations in all North Sea fish were significantly higher than English Channel and Irish Sea fish, but this difference disappeared when fish mass was taken into account. VTG concentrations showed no relationship to water depth, stage of sexual maturity or age of the males. Sixty selected male plasmas were assayed for 17beta-estradiol but only two had measurable amounts (assay limit 0.04 ng/ml). Despite being the start of summer, the gonads of many of the males and females (especially those caught in the North Sea) showed signs of sexual maturity (presence of sperm in males and vitellogenic eggs in females). Many females had high VTG concentrations (up to 14 mg/ml) and 78 out of 80 had measurable concentrations of 17beta-estradiol. The cause of elevated VTG levels in male dab is unknown. As seen in cod, the presence of affected males does not appear to be linked to proximity to land or to known point sources of endocrine disrupters. However, our data, showing that larger fish are more likely to have elevated VTG concentrations, suggests a gradual accumulation by marine fish, probably through feeding, of persistent (probably relatively weak) estrogenic compounds. 相似文献
13.
Yuji Tanaka 《Journal of Oceanography》1992,48(4):461-472
Near surface vertical distributions of Japanese anchovy eggs (isolated and pelagic) were studied at western Wakasa Bay, Japan. Samples were collected in horizontal tows with four plankton nets simultaneously operated at different four depths of 0, 0.5, 1.0, and 2.0 m. The egg concentration was found to decrease exponentially with depth. The egg concentration profile can be explained by considering the balance of the eggs' ascent and vertical diffusion. Taking further into account vertical difference in rising/sinking rates of the eggs, a clear accumulation of the eggs on a pycnocline, observed elsewhere, also can be explained. Vertical eddy diffusivity in the surface layer in a calm sea was 1–10 cm2 s–1, as estimated from the egg concentration profile and the velocities of the eggs in the vertical direction. 相似文献
14.
Abstract. The breeding biology of the cave-dwelling Mediterranean triplefin blenny Tripterygion melanurus (Pisces, Blennioidei) was studied in the field. Two types of reproductive male were observed, territorial males and non-territorial males. Territorial males establish territories for mating, but not necessarily for feeding, in caves and crevices at depths between 0.5 and 33 m. Territories can be settled in clusters in a single cave. Non-territorial males occur in the vicinity of territories and attempt to parasitise matings of territorial males. Gravid females wait near territories where another female is spawning, rather than spawn with currently unmated territorial males. Waiting females intrude in matings and sometimes displace a spawning female. The frequency of mating of the territorial male, of sneaking and of female intrusions are higher in territories that border others than in isolated territories. Females may minimise predation risks both on themselves and on their eggs by spawning in clustered territories and by spawning with males that have more than one clutch of eggs. Territorial males must find a compromise between the benefits of attracting a female and the costs of parasitic spawning by sneaker males. The possibility that territorial males tolerate the proximity of other territorial males and accept a risk of cuckoldry is discussed. Parasitic spawning in T. melanurus is relatively rare and the gonadosomatic index in both non-territorial and territorial males is low, indicating that sperm competition is probably not intense. 相似文献
15.
To determine the effect of low water temperature on development, walleye pollock (Theragra chalcogramma) eggs from the Bering Sea were reared at −0.6°C, 0.4°C, 2.0°C, and 3.8°C. One group of eggs was reared at 3.9°C under a diel light cycle (14 h light, 10 h dark) to observe the effect of light on development and hatching. Development was normal for all temperatures except −0.6°C; abnormal development of the tail and lack of development of eyes occurred in some embryos. Time to 50% hatch was 820, 620, and 424 h at 0.4°C, 2.0°C, and 3.8°C. Eggs incubated in diel light at 3.9°C developed at the same rate as eggs incubated in constant dark at 3.8°C, but required an additional 72 h to reach 50% hatch. A piece-wise regression model was generated to predict egg age for incubation temperatures of −0.6°C to 3.8°C. For temperatures recorded in the southeastern Bering Sea 1995–1998, the model predicted incubation periods for walleye pollock eggs that varied by 13 days between the warmest and coldest years.Walleye pollock eggs from Shelikof Strait, Alaska, were incubated at 0.2°C, 1.8°C, and 2.8°C. Development was normal for all temperatures. A piece-wise regression model (as above) was generated for incubation temperatures 0.2–2.8°C. When the regression models were compared, Bering Sea eggs (1.4–1.7 mm in diameter), required more time for development prior to hatch than Shelikof Strait eggs (1.2–1.3 mm in diameter) at 1.8°C and 2.8°C. However, for temperatures 0.2–2.0°C, Bering Sea walleye pollock began hatching earlier and at a developmentally younger age than Shelikof Strait walleye pollock. 相似文献
16.
运用电脉冲转基因仪对合浦珠母贝卵子进行电脉冲处理以优化电穿孔法转基因的电击参数。实验中调制百分比：100％；脉冲间隔：1．0s：电极距离：2mm；脉冲个数：5个，参数保持不变。用不同的电脉冲参数组合（脉冲电压100-400V、脉冲持续时间0．5～3．0ms、脉冲频率10～50kHz，共28个组合）对卵子电击，然后加入精子进行授精，统计各组受精率和孵化率，以受精率和孵化率达到50％以上为衡量指标。结果表明，合浦珠母贝卵子对低脉冲频率和高电压非常敏感，当频率低于20kHz或脉冲电压高于400V时对卵子损伤较大。脉冲持续时间对其影响不大，适应范围较宽。优化的转基因电击脉冲参教组合为：脉冲电压1.0kV／cm，脉冲持续时间20ms，脉冲频率50kHz，或者脉冲电压1．5kV／cm，脉冲持续时间1．5ms，脉冲频率50kHz。 相似文献
17.
对雌雄异体受精的东方扁虾的性腺发育，交配，排卵，抑卵孵育及孵化等繁殖习性进行了初步调研，同时也对闽南海区东方扁虾若干繁殖生物学参数进行了研究，结果表明：东方扁虾抱卵季节为3月初至10月中旬，高峰期是3月中锂至4月中锂，东方扁虾头甲长与体长之间的关系显示了雌雄二态现象，这一现象明显地与产卵特征相关联，抑卵雌虾的抑卵量（N）与体长呈曲线相关：N＝12.039(体长）^1.4813(r^2=0.7742)，东方扁虾在一年内可多次排卵，抱卵，卵径平均范围为0.96-1.29mm,卵重平均范围为0.575-1.000mg,卵径和个卵重都随胚胎发育的进展而增大。 相似文献
18.
Tributyltin and triphenyltin induce spermatogenesis in ovary of female abalone,Haliotis gigantea 总被引：1，自引：0，他引：1
Horiguchi T Kojima M Kaya M Matsuo T Shiraishi H Morita M Adachi Y 《Marine environmental research》2002,54(3-5):679-684
Two-month flow-through exposure experiments of tributyltin (TBT) and triphenyltin (TPhT) were conducted with abalone, Haliotis gigantea. Nominal concentrations of 100 ng TBT/l and 100 ng TPhT/l caused significant spermatogenesis in ovaries of exposed females. There were also significantly more contracted primary oocytes observed in females exposed to either TBT or TPhT than controls. The incidence of two types of unknown cells was also significant in females exposed to TPhT. No significant histological changes were observed in testis of exposed males. This ovarian spermatogenesis caused by TBT and/or TPhT resembles gastropod imposex. Remarkably high concentrations of TBT and TPhT were observed in the head (including central nervous system ganglia), compared to muscles concentrations. Accumulation of TBT and TPhT in the head may disturb reproductive hormonal regulators through neuropeptides released from ganglia. This, as well as possible aromatase inhibition, may be one of the inducers for spermatogenesis in the abalone ovaries. 相似文献
19.
Effects of dietary exposure to the pyrethroid pesticide esfenvalerate on medaka (Oryzias latipes) 总被引：3，自引：0，他引：3
Werner I Geist J Okihiro M Rosenkranz P Hinton DE 《Marine environmental research》2002,54(3-5):609-614
The pyrethroid insecticide esfenvalerate is widely used on orchard crops throughout California. In the aquatic environment, this compound is likely to accumulate in sediments, food particles and benthic organisms due to its lipophilicity and environmental persistence. This pilot project tested the hypothesis that esfenvalerate is toxic to medaka (Oryzias latipes) when taken up with the diet. For 7 days fish were fed diets, which contained esfenvalerate in three different concentrations (4, 21, 148 mg/kg, measured). Endpoints measured were mortality, fecundity, fertilization and hatching success of embryos, viability of larvae and cellular stress protein (hsp60, hsp70, hsp90) levels. The toxicity of aqueous exposure of medaka to esfenvalerate was also determined. Whereas the 96-h LC50 in the aqueous exposure was <9.4 microg/l, the dietary exposure did not cause mortality. Possible effects of dietary esfenvalerate were seen on fertilization and hatching success and the number of non-viable larvae. Expression of hsp60 and hsp90 showed a dose-dependent response pattern. 相似文献
20.
Population biology of the amphipod Ischyrocerus commensalis Chevreux, 1900, a facultative commensal of the red king crab Paralithodes camtschaticus (Tilesius, 1815), is here described on samples collected during summers 2005–2009 in Dalnezelenetskaya Bay (Southern Barents Sea, Russia). The studied features included infestation indices, size composition, morphometric, and reproductive parameters of the summer generation. During the studied period, prevalence and mean number of I. commensalis per host were 34.4% and 54.4 ± 3.3 individuals, respectively. The majority of amphipods were found on the host mouth parts, limbs, and gills. The amphipod sex‐ratio was significantly biased towards females (F:M = 1.36:1). The size frequency distributions observed in July and August were similar but in July the proportion of the smallest amphipods (<2.1 mm body length) was two times higher than that in August. All the amphipods with body length >5.1 mm were males or females. Length–weight relationships in female and male amphipods differed significantly, suggesting that females were heavier than the same‐sized males. The females carrying eggs in their brood pouches were the most abundant groups (54.8% in July 2009 and 51.6% in August 2005–2008). The minimal body length of a female which had released juveniles was 7.0 mm in July and 6.2 mm in August. The size at 50% maturity of I. commensalis females was estimated to be 6.58 mm. The number of eggs laid is positively linearly correlated with the size of a female. The proportion of the females with eggs in the latest developmental stage in July was 10 times higher than in August. The differences of biological features in symbiotic amphipods between the July and August may be explained by more intensive reproduction effort in the earlier month. 相似文献