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1.
A fast and indirect fluorescnet antibody assay for the Vibrio alginolyticus and V.Parahaemolyticus infecting the large yellow croaker has been developed.The specific antisera for the two strains of vibrio were prepared with New Zealand rabbit and the antiserum and cross-reactive efficacy was tested by coagulation in tube.It showed that the goat anti-rabbit IgG had been labeled by fuorescence isothiocyanate(FITC).The results showed that positive reactions were 100% for the large yellow croaker Pseudosciaena crocea with typical symptom of vibrio infection,while the positive reaction to the pathogen in healthy yellow croakers reached 40%,but seemed negative for aquaculter water.The results demonstrated that this fast and indirect fluorescent antibody assay can be used not only to test the vibrio pathogen in diseased yellow croaker but also in infected animals with no symptom. 相似文献
2.
Dietary leucine requirement for juvenile large yellow croaker, Pseudosciaena crocea Richardson 1846 (initial body weight 6.0 g ± 0.1 g) was determined using dose-response method. Six isonitogenous (crude protein
43%) and isoenergetic (19 kJ g−1) practical diets containing six levels of leucine (Diets 1–6) ranging from 1.23% to 4.80% (dry matter) were made at about
0.7% increment of leucine. Equal amino acid nitrogen was maintained by replacing leucine with glutamic acid. Triplicate groups
of 60 individuals were fed to apparent satiation by hand twice daily (05:00 and 17:30). The water temperature was 26–32°C,
salinity 26–30 and dissolved oxygen approximately 7 mg L−1 during the experimental period. Final weight (FW) of large yellow croaker initially increased with increasing level of dietary
leucine but then decreased at further higher level of leucine. The highest FW was obtained in fish fed diet with 3.30% Leucine
(Diet 4). FW of fish fed the diet with 4.80% Leucine (Diet 6) was significantly lower than those fed Diet 4. However, no significant
differences were observed between the other dietary treatments. Feed efficiency (FE) and whole body composition were independent
of dietary leucine contents (P > 0.05). The results indicated that leucine was essential for growth of juvenile large yellow croaker. On the basis of FW,
the optimum dietary leucine requirement for juvenile large yellow croaker was estimated to be 2.92% of dry matter (6.79% of
dietary protein). 相似文献
3.
4.
We evaluated the immune response to infection with a pathogen in large yellow croaker(Pseudosciaena crocea Richardson).The fish were given an intraperitoneal(i.p.) injection of Vibrio parahaemolyticus or sterile sea water(control).We collected blood sera from the fish 0.17,1,2,4,8,12,or 16 d after injection(dpi).We measured tyrosinase activity and the concentrations of lysozyme,NOS,and antibodies.Serum tyrosinase activity was significantly higher at 0.17 and 4 dpi than in the control group,and peaked at 8 d... 相似文献
5.
We estimated the reproductive success of adult brood fish and the heritability of growth-related traits for large yellow croaker (Larimichthys crocea). We created two independent full-factorial cross groups (1 and 2) by crossing 4 males×4 females and 4 males×3 females, respectively. We measured the body weight (BW), body length (BL), and body height (BH) of 281 individuals from group 1 and 318 individuals from group 2 at 20 months post hatch (harvest age). We also collected a tissue sample from each individual. The parents and offspring were genotyped using six polymorphic microsatellites. Of the 599 offspring, 99.2% were assigned to a single pair of parents. In both groups, some parent pairs produced a large number of offsprings while other pairs did not produce any offspring. The genetic diversity and putative Ne were lower in the offsprings than in the parents in both groups. The heritability estimates at 20-month age were 0.13±0.10 for BW, 0.19±0.13 for BL, and 0.09±0.06 for BH. The genetic and phenotype correlation between BW, BL, and BH was close to 1. The results provide basic information for selective breeding and further genetic characterization of large yellow croaker. 相似文献
6.
7.
For the investigation of anti-infection immune response of Pseudosciaena crocea, 160 healthy fish samples were categorized into infected and control groups. Each individual fish in the infected group was injected intraperitoneally (i.p.) with 0.2 ml bacterial suspension of Vibrio alginolyticus in density of 2×107 CFU/ml, while each individual in the control group was injected i.p. with 0.2 ml sterile saline solution (0.85%). It was observed that the artificial injection of V. alginolyticus significantly inc... 相似文献
8.
采用微生物分离培养技术和巢式PCR技术相结合方法对2011年采自广西北海南万和古城、钦州茅岭和钦州港、防城港企沙和公车的拟穴青蟹(Scylla paramamosain)携带寄生虫、细菌和病毒状况开展调查.寄生虫检测结果显示,仅钦州茅岭拟穴青蟹鳃组织中发现寄生虫茗荷(儿).细菌性病原体检测结果显示,共有11种病原性细菌存在于健康养殖拟穴青蟹体内,弧菌属细菌性病原出现频率最高,其中副溶血弧菌和溶藻弧菌在调查区域内均有分布,其他菌种则呈现一定的地域特异性.病毒性病原体检测结果显示,白斑综合症病毒仅在钦州茅岭拟穴青蟹检出,阳性率为4.94%;双顺反子病毒于北海古城和钦州茅岭拟穴青蟹检出阳性携带率为3.70%;呼肠孤病毒携带率则较高,北海、防城港以及钦州拟穴青蟹均有检出,阳性率达25.93%.由此可见,10-11月广西区内健康养殖拟穴青蟹携带弧菌和呼肠孤病毒的比例远远高于其他病原体,为拟穴青蟹携带的主要病原体. 相似文献
9.
ＩＮＴＲＯＤＵＣＴＩＯＮＴｈｅｌａｒｇｅｙｅｌｌｏｗｃｒｏａｋｅｒＰｓｅｕｄｏｓｃｉａｅｎａｃｒｏｃｅａｏｎｃｅｗａｓｏｎｅｏｆｔｈｅｆｏｕｒｉｍｐｏｒｔａｎｔｍａｒｉｎｅｆｉｓｈ ｅｒｙｏｂｊｅｃｔｓｉｎＣｈｉｎａ (Ｈｏｎｇｅｔａｌ.,1 983;ＨｕａｎｇａｎｄＣａｒｌ,1 983;ＣｈｅｎａｎｄＧｏｎｇ ,1 983) .ＴｈｅｐｒｏｄｕｃｔｉｏｎｏｆｔｈｅｗｉｌｄＰ .ｃｒｏｃｅａｉｎ 1 974ｒｅａｃｈｅｄ 1 971 78ｍｅｔｒｉｃｔｏｎｓ (ＭＴ)ｉｎＣｈｉｎａ (Ｌｉ,1 998) .Ｂｅｃａｕｓｅｏｆｏｖｅｒｆｉｓｈｉｎｇ ,ｔｈ… 相似文献
10.
Current status of small yellow croaker resources in the southern Yellow Sea and the East China Sea 总被引：3，自引：1，他引：2
We used data from bottom trawl surveys to study the factors influencing the abundance of small yellow croaker, Larimichthys polyactis, in the southern Yellow Sea (SYS) and the East China Sea (ECS). The resource density index (RDI) was generally higher in
summer and autumn than in spring and winter. RDIs were also significantly greater in the SYS than in the ECS in summer and
autumn. The bottom water salinity and depth of spatial distribution of small yellow croaker was similar between the two areas
in summer, but different in other seasons. Regression analysis suggested that environmental factors such as bottom water temperature,
salinity, and depth influenced the RDIs in summer in these areas. Growth condition factor (GCF) in the two areas varied monthly
and the croaker in the SYS grew more slowly than those in the ECS. This was likely due to the low bottom temperature of the
Yellow Sea Cold Water Mass in summer and autumn or to higher human fishing pressure in the ECS. To ensure sustainable utilization
of the croaker stocks in these regions, we recommend reducing the fishing intensity, increasing the cod-end mesh size, and
improving the protection of juveniles. 相似文献
11.
Single nucleotide polymorphisms in intron 1 and intron 2 of Larimichthys crocea growth hormone gene are correlated with growth traits 总被引：1，自引：0，他引：1
The growth hormone gene (GH) affects animal growth and is a potential target for genetic studies of variation related to growth traits. In this study, we analyzed single nucleotide polymorphisms (SNPs) in GH intron regions and their associations with growth traits in large yellow croaker, Larimichthys crocea, from Zhejiang and Fujian stocks. The results of PCR-single strand conformation polymorphism showed two haplotypes of intron 1, named AA and AB genotypes, in Zhejiang stock. AB exhibited an SNP at position 196 (G A) that was negatively correlated with body height and positively correlated with standard length/body height (P 0.05). Two different genotypes, CC and CD, were identified in intron 2 in Fujian stock, with CD showing an SNP at position 692 (T C). The CD genotype had a significantly positive correlation with both weight and total length (P 0.01). These basic data highlight the potential for using GH as a genetic marker of fish growth in marker assisted selection. 相似文献
12.
The present study investigated conditions for inducing mito-gynogenetic(endomitosis) diploids by hydrostatic pressure in the large yellow croaker Pseudosciaena crocea.In haploid control groups,the development of eggs was activated with ultraviolet radiated semen.All fry presented typical haploid syndrome in the haploid control groups,and were verified as haploids using cytometry.After hydrostatic pressure treatment,morphologically normal fry reappeared at different frequencies according to the intensity and... 相似文献
13.
Large areas of hypoxic water have recently been reported in the East China Sea. It is hypothesized that hypoxia may be partially responsible for the decline of some fish stocks. We evaluated the effect of hypoxia on large yellow croaker (Pseudosciaena crocea). The fish were exposed to three concentrations of dissolved oxygen (DO; 1.5 mg/L and 2.0 mg/L, and 6.5 mg/L control). We collected blood after 6, 12, 24, 48, and 96 h exposure. There was a significant increase in red blood count, hematocrit, hemoglobin concentration, and mean corpuscular hemoglobin in the group exposed to 1.5 mg/L DO after 6 h or 12 h, and a delayed increase (only elevated after 48 h and 96 h) in these indices in the group exposed to 2.0 mg/L DO. Plasma glucose concentrations increased significantly in both hypoxic groups after 24 h. Furthermore, plasma lactate and lactate dehydrogenase activity increased significantly after the first 6 h exposure in both hypoxic groups. Our results suggest that large yellow croakers could not maintain the aerobic pathway and instead use anaerobic metabolism for survival when DO levels fall below 2.0 mg/L. We conclude that the occurrence of hypoxia (<2 mg/L DO) in the East China Sea could cause metabolic stress for large yellow croakers and may be partially responsible for the population decline of this species. 相似文献
14.
A large yellow croaker,Pseudosciaena crocea,spleen(LYCS)cell line was established and the feasibility of using it for foreign gene transfection was evaluaed in this study.Primary culture of LYCS cells was initiated from spleen tissue pieces,which were cultured at 25℃ in Dulbecco’s modiced Eagle medium/F12 medium(DMEM/F12,1:1)(pH7.2),supplemented with 20% fetal bovine serum,carboxymethyl chitosan,chondroitin sulfate,basic fibroblast growth factor(bFGF)and insulin-like growth factor-I(IGF-I).The cultured LYCS cells,in fibroblast shape,proliferated to 100% confluency 20 days later.Chromosome analyses indicated that the LYCS cells exhibited chromosomal aneuploidy with a modal chromosome number of 48 which displayed the normal diploid karyotype of P.crocea(6m+6sm+36t,NF=60).A LYCS cell line,with a population doubling time of 48.7 h at passage 60,has been established and subcultured to passage 70.Transgenic feasibility test demonstrated that positive green fluorescence protein(GFP)expression was observed in LYCS cells after pcDNA3.1-GFP plasmid transfection.In conclusion,a continuous foreign gene trans-fection feasible LYCS cell line has been established successfully.The cell line might serve as a valuable tool for studies of transgenic breeding and has potential applications for different kinds of cytotechnological studies. 相似文献
15.
ARTIFICIAL PROPAGATION AND BREEDING OF MARINE FISH IN CHINA 总被引：3，自引：0，他引：3
Since the 1990s, artificial propagation and breeding technique of marine fish in China have developed by way of increasing species and fry numbers, with special stress laid on valuable species. Large quantities of artificial fry can meet the needs of both marine cage culture and pond culture for most species. Experimental results obtained by scientists have been put into use in actual production.Fish fry production has entered a period of sustainable development. So far, at least 44 species (21 families) of marine fish have been successfully bred in China. The artificial fry number of large yellow croaker ( Pseudosciaena crocea) exceeded 300 million in 1999. The species whose artificial fry numbers have each surpassed 10 million annually are red drum ( Sciaenops ocellatus ), Japanese seabass ( Lateolabrax japonicus), cuneate drum (Nibea miichthioides ), spring spawning red seabream (Pagrosomus major ) and threebanded sweethp ( Plectorhynchus cinctus ). Millions of artificial fry are bred annually in the species of black porgy ( Sparus macrocephalus ), Russell‘ s snapper ( Lutjanus russeUi ), javelin gnmt ( Pomadasys hasta ), miiuy croaker ( Miichthys miiuy) and skewband gnmt ( Hapalogenys nitens ). The fish in the family Sciaenidae are the main species in artificial propagation and breeding. Some problems and prospects on marine fish culture and stock enhancement are also discussed and some proposals for sustainable development are put forward in this article. 相似文献
16.
用鲶爱德华氏菌兔抗血清作为一抗,碱性磷酸酶(AP)标记的羊抗兔IgG作为酶标二抗,建立黄颡鱼"红头病"病原菌—鲶爱德华氏菌的间接酶联免疫(ELISA)快速检测法,并优化检测条件。抗原最佳包被浓度为107/mL,一抗工作的最佳稀释度为1∶211,病原菌的检测灵敏度为105/mL,交叉反应实验证明该方法特异性强,与迟钝爱德华氏菌、弧菌等13种标准菌株无交叉。应用上述技术对人工感染发病鱼中分离的优势菌进行检测,结果表明阳性检出率为80%;对自然发病黄颡鱼体内分离获得的20株优势菌检测结果表明,12株菌为鲶爱德华氏菌。 相似文献
17.
In order to determine the effect of twine thickness on the size-selectivity of the driftnet used for the yellow croaker, size-selectivity tests were conducted with three different twine thicknesses (monofilament diameters of 0.279 mm (number’s method; No. 3), 0.321 mm (No. 4), and 0.360 mm (No. 5)) of driftnets for the yellow croaker in the seas around Chooja-do, Jeju Islands. The selectivity curve was estimated by using Kitahara’s method. In order to determine the physical properties of the twine used in the experimental fishing nets, we measured the breaking load, elongation, and stiffness under both dry and wet conditions. In terms of physical properties, the thinnest twine (No. 3) had the strongest breaking strength per unit cross-sectional area, along with good elongation and excellent flexibility. The thickest twine (No. 5) had the lowest flexibility. In terms of selectivity, the net of No. 3 twine showed the broadest selection range and, thus, a relatively low selectivity compared with the other nets, while the less flexible net of No. 5 twine showed the narrowest selectivity range and high selectivity. In addition, it was found that a thicker twine resulted in a smaller haul of small fish. Therefore, it can be inferred that the thickness of the twine affects the size of the catch and selectivity, and thus the size composition of the catch as well. 相似文献
18.
《中国海洋湖沼学报》2016,(6)
In order to determine the effect of twine thickness on the size-selectivity of the driftnet used for the yellow croaker, size-selectivity tests were conducted with three different twine thicknesses(monofi lament diameters of 0.279 mm(number's method; No. 3), 0.321 mm(No. 4), and 0.360 mm(No. 5)) of driftnets for the yellow croaker in the seas around Chooja-do, Jeju Islands. The selectivity curve was estimated by using Kitahara's method. In order to determine the physical properties of the twine used in the experimental fi shing nets, we measured the breaking load, elongation, and stiffness under both dry and wet conditions. In terms of physical properties, the thinnest twine(No. 3) had the strongest breaking strength per unit cross-sectional area, along with good elongation and excellent fl exibility. The thickest twine(No. 5) had the lowest fl exibility. In terms of selectivity, the net of No. 3 twine showed the broadest selection range and, thus, a relatively low selectivity compared with the other nets, while the less fl exible net of No. 5 twine showed the narrowest selectivity range and high selectivity. In addition, it was found that a thicker twine resulted in a smaller haul of small fi sh. Therefore, it can be inferred that the thickness of the twine affects the size of the catch and selectivity, and thus the size composition of the catch as well. 相似文献
19.
The genetic diversity of both wild and reared Pseudosciaena crocea (Richardson) col-lected from Guan-Jing-Yang in Ningde, China in May 1999 was investigated by random amplified poly-morphic DNA (RAPD) in the present study. The polymorphism and mean difference of the wild popula-tion as revealed by RAPD were 18.9% and 0.0960 respectively, and those of the reared stocks were rel-atively lower, with 16.7% in polymorphism and 0.0747 in mean difference. The genetic distance be-tween the two stocks was 0.0041. From the comprehensive investigation, the main reasons for the loss of genetic diversity were probably overilshing, small number of parents as broodstocks and the debatable arti-ficial ranching. Results from this study also showed that the large yellow croaker populations distributed along Fujian coastal waters including Guan-Jing-Yang still potentially wide genetic variability. It is sug-gested that genetic management and prevention should be scientifically conducted in order to maintain and improve the genetic diversity of the P. crocea population. 相似文献
20.
For the investigation of anti-infection immune response of Pseudosciaena crocea, 160 healthy fish samples were categorized into infected and control groups. Each individual fish in the infected group was
injected intraperitoneally (i.p.) with 0.2 ml bacterial suspension of Vibrio alginolyticus in density of 2×107 CFU/ml, while each individual in the control group was injected i.p. with 0.2 ml sterile saline solution (0.85%). It was
observed that the artificial injection of V. alginolyticus significantly increased the number of erythrocytes, leucocytes, lymphocytes in peripheral blood as well as peripheral serum
antibacterial activity and antibody titer of large yellow croaker, and significantly reduced the number of peripheral blood
granulocytes as compared with those in the control group. No significant difference in acid phosphytase and superoxide dismutase
activity of serum was detected between the two groups. It is suggested that non-specific immune factors including leucocytes
and anti-bacteria substance in peripheral blood played important role at the initial stage of infection, and specific immune
factors such as antibody then played important role in response to anti-infection at the latter stage.
Supported by the National High Technology Research and Development Program of China (863 program, No. 2007AA09Z115) and Technology
Program of Xiamen (No. 3502Z73019) 相似文献