Concentration of cementation solution (CCS) is one of the key factors influencing the cementation effect on soil improvement through the microbially induced carbonate precipitation (MICP) process. To precipitate more calcium carbonate per treatment, a higher CCS is needed. However, the MICP process may be retarded or even terminated with an increase in CCS. This retarding effect can be a major limitation for the MICP-based soil treatment and thus needs to be understood properly. This paper presents a systematic study on the conditions causing retarding and its effect on biocementation. The test results of this study have identified that there is retarding effect of CCS on the MICP process, showing that the calcium conversion efficiency, which represents the amount of calcium that has been converted into calcium carbonate in each treatment, reduces with the increase in CCS, and the concentration of calcium is the control factor. The retarding effect will dominate increasingly when CCS is higher than 1.0 M and the amount of calcium carbonate precipitation will reduce for the given amount and type of bacteria used in this study and become zero with CCS of 2.5 M. For the same calcium carbonate content, the unconfined compressive strength is greater for sand treated using a lower CCS as the contribution to the bonding strength by the calcium carbonate generated under a lower CCS is greater than that under a higher CCS.
相似文献Microbially induced calcium carbonate (CaCO3) precipitation (MICP) has been extensively studied for soil improvement in geotechnical engineering. The quantity and size of calcium carbonate crystals affect the strength of MICP-treated soil. In this study, microfluidic chip experiments and soil column experiments were conducted to optimize MICP treatment protocols for effective strength enhancement of MICP-treated sandy soils. The microscale experiments reveal that, due to Ostwald ripening, longer injection intervals allow crystals to dissolve and reprecipitate into larger crystals regardless of the concentration of cementation solution. Even though a cementation solution input rate of 0.042 mol/l/h is sufficient to maintain a high chemical transformation efficiency, a further reduction in the input rate by about four times resulted in an increase in the size of crystals produced by the end of treatment from about 40 to 60 μm. These findings were applied in soil column experiments. Results showed that significantly larger crystals and higher soil strength were achieved when the normalized rate of cementation solution injection was reduced from 0.042 to 0.021 mol/l/h. Crystal size and soil strength increased slightly more when the normalized input rate was further reduced from 0.021 to 0.010 mol/l/h. This study demonstrates how data from microscale microfluidic experiments that examine the effects of injection intervals and concentration of cementation solution on the properties of calcium carbonate crystals can be used to optimize MICP treatment in macroscale sand soil column experiments for effective strength enhancement.
相似文献Kitchen waste and wind erosion are two worldwide environmental concerns. This study investigated the feasibility of using kitchen waste for Sporosarcina pasteurii cultivation and its application in wind erosion control of desert soil via microbially induced carbonate precipitation (MICP). Enzymatic hydrolysis was adopted to improve the release and recovery of protein in kitchen waste for subsequent microorganism production. After conditions optimized, the maximum biomass concentration (OD600) and urease activity of Sporosarcina pasteurii in the kitchen waste-based medium reached 4.19, and 14.32 mM urea min?1, respectively, which were comparable to those obtained in conventional standard media. The harvested Sporosarcina pasteurii was then used to catalyze the precipitation of calcium carbonate in the desert soil, and its performance in wind erosion control was evaluated through wind tunnel tests. The microbially mediated calcium carbonate could significantly decrease wind erosion loss of the desert soil even after 12 wet–dry or freeze–thaw cycles. Scanning electron microscopy (SEM) with energy-dispersive X-ray (EDX) confirmed the bridge effect of calcium carbonate crystals in the soil matrix. The kitchen waste, as a cost-effective alternative nutrient for bacterial cultivation and carbonate precipitation, showed great potential for large-scale applications in wind erosion control of desert soils.
相似文献The use of biopolymer to improve the performance of microbially induced carbonate precipitation (MICP)-treated sands is a novel and eco-friendly concept. This work found an anionic biopolymer, γ-polyglutamate (γ-PGA), could significantly improve the performance of MICP-treated sands. Comparing the control with absence of γ-PGA, the concentration of 0.1–9 g/L γ-PGA increased the compressive strength of MICP-treated sands by 1.54–3.96 times and significantly reduced the brittleness. The MICP process analysis and microstructural detection demonstrated that γ-PGA in the specimens provided many nucleation sites and templates for calcite generation, partially kept the bacterial urease activity by replacement of the bacteria as nucleation sites, thereby improving the calcite generation. The γ-PGA also cemented sand grains with calcite through the hydrogen bond-type intermolecular interactions. Both the calcite generation and the hydrogen bond-type intermolecular interactions by γ-PGA played vital roles in enhancing MICP for soil improvement. Additionally, γ-PGA, as a viscoelastic admixture between the crystals and sand grains, effectively dissipated the energy of stress and thus reduced the brittleness of MICP-treated sands. This is the first report on the application of anionic biopolymer to MICP technology. It provides a novel concept in promoting the efficiency and sustainability of MICP.
相似文献Microbial induced calcite precipitation (MICP), a bio-cementation process, can be adopted to improve the engineering properties of granular soils. Bio-stimulation, via directly enriching indigenous ureolytic bacteria, is a sustainable and economical approach to achieve MICP. In this study, batch solution experiment was firstly conducted to investigate the biochemical aspects of the bio-stimulated MICP process in coral sands. Three different enrichment media were compared. The statistical analysis was performed to reveal statistically significant factors that influence ureolytic activity, pH value, and viable cell number. Then, the unconfined compression and rainfall-induced erosion tests were conducted to investigate the strength and erosion-resistance of bio-stimulated MICP treated coral sands. The experimental results demonstrate that the enrichment duration, initial urea concentration, and enrichment type are major influencing factors of the ureolytic activity. It is found in this study that yeast-extract-based enrichment media with 170 mM initial urea concentration and enriched for 72 h could achieve the best bio-stimulated MICP treatment efficiency. In addition, higher initial urea concentration in the enrichment medium could yield higher ureolytic activity, which could consequently result in higher cementation content and thus larger UCS and better resistance to rainfall-induced erosion.
相似文献