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1.
海洋微藻脂肪酸的气相色谱分析   总被引:5,自引:0,他引:5  
以正十九酸(C19:0作内标,藻体经超声萃取、浓缩吹干,进行抽提酯化后采用毛细管气相色谱法分析测定。该方法重现性好,相对偏差为0.53%-5.49%,回收率较高,为95.1%-102.5%,检测限为0.0072μg。  相似文献   

2.
鲈鱼土池人工育苗研究   总被引:3,自引:0,他引:3  
报道了1989-1993年鲈鱼土池人工育苗的研究结果,捕自天然海区、围垦内 网箱培育的亲鱼注射LRH-A与HCG诱导能顺利排卵,产卵率70-100%,受精卵卵径1.25-1.40mm,浮性卵、受精率21-89.0%,在水温18.0-21.2℃、盐度31.0-34.0的条件下,一般经50.67h孵出仔鱼,孵化率46.0-90.0%。人工育苗在室外土池进行,最高成活率11.7%。初孵仔鱼全长3.53-  相似文献   

3.
用QSAR法研究有机磷农药对海洋扁藻的构效关系   总被引:8,自引:2,他引:6  
邹立  李永祺 《海洋与湖沼》1999,30(2):206-211
1997年3月,利用QSAR构效关系分析方法和计算模拟手段,对中国洞海地区常用的11种有机磷农药对海洋五心形扁藻拦数抑制浓度进行研究。建立有机磷农药对海洋扁藻致毒效应的QSAR模型;lg(1/EC50)=0.193lgP-0.08(lgP)^2+1.10σ3+0.292^1X-0.164(n=11,S=20,R=0.9696);lg(1/EC50_=0.191lgP-0.09(lgP)^2+1.0  相似文献   

4.
三种胡子鲶的遗传变异及其系统分类的研究   总被引:1,自引:0,他引:1  
庆宁  赵迪 《热带海洋》1993,12(4):88-94
为研究三种胡子鲶(胡子鲶Clarias fuscus、两栖胡子鲶C.batrachus和革胡子鲶C.lazer-a)遗传变及系统分类关系,采用淀粉凝胶电泳技术比较了三种胡子鲶4个样本的30-31个基因座位。每个座位观测到的平均等位基因数为1.01-1.39;多态座位百分比P0.99为3.33-16.13%,平均杂合度观测值为0.004-0.072;期望值为0.004-0.064。3个种的遗传变异较  相似文献   

5.
用磁化水培养等鞭藻3011的初步研究   总被引:6,自引:0,他引:6  
本文主要报道不同磁场强度的磁化水对等鞭藻繁殖作用的影响。1材料和方法安排5组磁场强度,分别为0.05,0.1,0.3,0.6,0.9T,一个对照组。5000ml的三角烧瓶6个。对海水进行沉淀、沙滤、陶瓷过滤,经磁化水器后,即为磁化水(不煮沸),尔后加入营养盐NaNO360mg;KH2PO44mg;FeC6H5O0.5mg;NaSiO35mg;VB1100ug;VB120.5ug;溶于1000ml海水中煮沸。室温15~18.5℃,光照240~21601X,每天24h,,培养期间每天搅瓶6次。每天上…  相似文献   

6.
条斑紫菜对无机碳的利用   总被引:4,自引:0,他引:4  
于1998年3月在青岛太平角采集条斑紫菜,在室内光照强度为120μE/(m~2·s)的培养架上培养,通过测定其光合作用引起密闭海水体系pH值及无机碳浓度的变化,借助于各种无机碳利用的抑制剂(Az、Van、DIDS、SITS)对其无机碳利用机制进行了研究。结果表明,(1)条斑紫菜可间接吸收HCO~(-)_(3)。胞外碳酸醉酶(CA)催化HCO~(-)_(3)水解成CO_2扩散进入胞内是条斑紫菜无机碳利用的主要形式,占全部无机碳利用的73%以上。Az是胞外CA的抑制剂,可直接检测胞外CA的存在,它不能透过细胞膜。(2)条斑紫菜可依赖于细胞膜上ATP酶对HCO~(-)_(3)直接转运。但未发现带Ⅲ蛋白(band 3 Protein)及 Na~+/HCO_(3)~(-)协同转运系统的参与。(3)条斑紫菜pH值补偿点为9.83,CO_2补偿点为0.04μmol/L。  相似文献   

7.
河口红树林湿地CH_4通量的日变化研究   总被引:1,自引:0,他引:1  
1引言 甲烷(CH4)是大气中除 CO2外最为丰富的含碳组分,其浓度以每年 0. 7%- 1. 1%的速率递增[1,2].尽管大气 CH4含量仅为 CO2的二百分之一,但却对预计的全球变暖约有 20%的贡献率,而CO2的贡献率约为50%[3,4],由这些数据推算得一个分子的CH4比一个分子的CO2(目前最重要的温室气体)的增暖潜值高约80倍. 很多研究者对全球大气CH4的预算结果均表明,湿地是大气 CH4最重要的生物源[5,6],约占全球 CH4源的 40%~50%[7].湿地 CH4通量的日变化研究是正确估…  相似文献   

8.
海水pH对疏浚物中重金属释放的影响   总被引:2,自引:0,他引:2  
廖文原  陈松 《台湾海峡》1994,13(4):388-393
对厦门员当湖污染疏浚物中的Cd,Cu和Pb在不同pH的天然海水中溶出的模拟研究结果表明:在pH为5.0,6.5,8.3条件下,疏浚物中的Cd,Cu,Pb的释放率分别为80%-94%,4%-9%和1.0%-2.2%;在相同条件下,Cd,Cu,Pb的释放量(释放量)均随pH值增大而减少,释放量的顺序为Cd>Cu>Pb。讨论了pH对释放作用的影响及释放过程所遵循的动力学规律。  相似文献   

9.
高淑英 《海洋通报》1995,14(4):38-42
1990年5月湄州海域溶解态Cu,Pb,Cd的含量范围分别为0.51-1.68、0.037-0.964和0.010-0.073μg/dm^3,10月的对应值分别为0.48-1.25,0.019-0.989和0.020-0.045μg/dm^3。5月和10月Cu,Pb,Cd含量的分布除了与不同水系消长的影响有关外,还与人类活动和生物因素的影响有关。  相似文献   

10.
本文介绍了一种测定积物中^210Pb的方法。沉积物中的^210Pb用HNO3和HF消化,然后^210Po在85-90℃下从0.5molHCL介质中自沉积在银片上;其全程放化回收率为85.0±4.9×10^-2。  相似文献   

11.
In order to assess the impact of deep-sea mining on the in situ benthic life, we measured the microbial standing stock and concentration of organic nutrients in the deep-sea sediments of the Central Indian Ocean Basin in the Indian pioneer area. Sediments were collected using box core and grab samples during September 1996. The total bacterial numbers ranged from 10 10 -10 11 cells per g -1 dry weight sediment. There was a marginal decrease in the number of bacteria from surface to 30 cm depth, though the subsurface section registered a higher number than did the surface. The highest numbers were encountered at depths of 4-8 cm. The retrievable number of bacteria were two orders less in comparison with the direct total counts of bacteria. An almost homogeneous distribution of bacteria, total organic carbon, living biomass, and lipids throughout the depth of cores indicates active microbial and benthic processes in the deep sea sediments. On the other hand, a uniform distribution of total counts of bacteria, carbohydrates, and total organic carbon in all the cores indicates their stable nature and suggests that they can serve as useful parameters for long-term monitoring of the area after the benthic disturbance. Further studies on temporal variability in this region would not only verify the observed norms of distribution of these variables but would also help to understand restabilization processes after the simulated benthic disturbance.  相似文献   

12.
The interdependence between the seismo-acoustic properties of a marine sediment and its geotechnical/physical parameters has been known for many years, and it has been postulated that this should allow the extraction of geotechnical information from seismic data. Though in the literature many correlations have been published for the surficial layer, there is a lack of information for greater sediment depths. In this article, a desktop study on a synthetic seafloor model illustrates how the application of published near-surface prediction equations to subsurface sediments (up to several tens of meters burial depth) can lead to spurious predictions. To test this further, acoustic and geotechnical properties were measured on a number of sediment core samples, some of which were subjected to loading in acoustically-equipped consolidation cells (oedometers) to simulate greater burial depth conditions. For low effective pressures (representing small burial depths extending to around 10 meters subsurface), the general applicability of established relationships was confirmed: the prediction of porosity, bulk density, and mean grain size from acoustic velocity and impedance appears generally possible for the investigated sedimentary environments. As effective pressure increases through, the observed relationships deviate more and more from the established ones for the near-surface area. For the samples tested in this study, in some instances increasing pressure even resulted in decreasing velocities. There are several possible explanations for this abnormal behavior, including the presence of gas, overconsolidation, or bimodal grain size distribution. The results indicate that an appropriate depth correction must be introduced into the published prediction equations in order to obtain reliable estimates of physical sediment properties for greater subsurface depths.  相似文献   

13.
An acoustic inversion method using a wide-band signal and two near field receivers is proposed and applied to multiple layered seabed models including a manganese sediment. The inversion problem can be formulated into a probabilistic model comprised of signals, a forward model, and additive noise. The forward model simulates wide-band signals, such as chirp signals, and is chosen to be the source-waveletconvolution plane wave modeling method. The wavelet matching technique, using weighted least-squares fitting, estimates the sediment sound-speed and thickness on which determination of the possible numerical ranges for a priori uniform distribution is based. The genetic algorithm is applied to a global optimization problem to find a maximum a posteriori solution for determined a priori search space. Here the object function is defined by an L 2 norm of the difference between measured and modeled signals. Not only the marginal pdf but also its statistics are calculated by numerical evaluation of integrals using the samples selected during importance sampling process of the genetic algorithm.  相似文献   

14.
海上大直径钢管桩打桩过程中,桩周土体受到强烈扰动而发生强度弱化,掌握桩周土体强度弱化规律对于准确预测打桩过程、保证工程安全具有重要意义。为研究土体强度弱化规律,开展了环剪试验模拟打桩对桩周土体的扰动,测试土体强度随剪切速率的变化规律,建立了描述土体强度弱化规律的拟合公式,引入到打桩分析软件中。研究结果表明:土体的强度折减程度不仅与土体本身的性质有关还受到土体的埋深和剪切速率的影响,埋深越深土体强度折减程度越低,剪切速率越高土体强度折减越高,在打桩分析中可采用这里推荐的线性折减方法来模拟不同深度处土体强度的折减规律。  相似文献   

15.
Specific properties of the interannual sea level variations and annual tides in the Northwestern Pacific were studied. Several tide stations were monitored. The monthly mean sea level for the year of 1995 was analyzed at each tide station. A seismic event in 1995, some tectonic activity around the subject area, and the Kuroshio (the oceanic western boundary current) may possibly contaminate results which would have occurred from the astronomical annual tide alone.  相似文献   

16.
The effect of different fixation and storage protocols on the flow cytometric (FCM) simultaneous analysis of bacterioplankton and phytoplankton in coastal seawater samples (Mediterranean coastal lagoons) was investigated. FCM measurements (cell number, fluorescence and scatter characteristics) were obtained through DAPI staining. Three fixatives [glutaraldehyde (GA), formaldehyde (FA) and paraformaldehyde (PFA)] and two storage (3 months duration) methods (5 °C and −196 °C) were tested. Two dominant populations were detected in studied samples: bacteria and eukaryotic picophytoplankton. Adding fixatives (2% final concentration) appears necessary to obtain FCM exhaustive counts of all the bacteria and phytoplanktonic cells. This was related to the permeation effect of fixatives which allowed a better DAPI staining of the cells. Maximum fluorescence, i.e. optimal staining of the cells was obtained with FA or PFA, and significant lower fluorescences with GA. Fixed samples stored at 5 °C induced rapid cell loss. Only storage in liquid nitrogen of samples fixed with FA or PFA, allows mid-term (≥4 months) preservation of bacteria or picophytoplankton cell numbers, and limited evolution of DAPI-induced fluorescence and scatter characteristics.  相似文献   

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19.
《海洋学报(英文版)》2014,(9):F0003-F0003
<正>Acta Oceanologica Sinica(AOS)is a comprehensive academic journal edited by the Editorial Committee of Acta Oceanologica Sinica and is designed to provide a forum for important research papers of the marine scientific community which reflect the information on a worldwide basis.The journal publishes scholarly papers on marine science and technology,including physics,chemistry,biology,  相似文献   

20.
Oedometer tests have been carried out on 70 undisturbed surficial clays (at approximately 250 mm below the mudline), mostly collected by free-fall corers from sites widely scattered throughout the deep-sea North Atlantic. Acoustic measurements were also made, initially on contiguous samples and ultimately on the same sample using a geophysically instrumented oedometer which also collected electrical resistivity data. Apart from those quiescent areas below the carbonate compensation depth, such as north of the West Indies where very fine clays exist, most of the samples are silty clays whose geotechnical-geophysical properties are dependent on the type of clay minerals present (and their ability to take in moisture), the sand-size fraction, and the quantity of carbonate present. Thus the pure clays have high compressibilities which decrease on the addition of coarse particles, while the converse is true for the acoustic parameters, these increasing with the sand fraction. Using the notion of the intrinsic compression line for all samples, and comparison to it of the measured compression curves, it is clear that, contrary to some previously held ideas, most deep-sea clays are normally consolidated; the addition of carbonate has the effect of creating an open, stronger sediment skeleton. Interestingly, where information is available, the variation with depth of a sample's acoustic velocity follows the void ratio pressure relationship of the compression curve. This allows the construction of an in-situ sediment compression curve using the in-situ geophysical observations.  相似文献   

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