共查询到20条相似文献，搜索用时 31 毫秒
1.
ＩＮＴＲＯＤＵＣＴＩＯＮＧｒｏｗｔｈｈｏｒｍｏｎｅ (ＧＨ)ｉｓａｐｏｌｙｐｅｐｔｉｄｅｐｒｏｄｕｃｅｄｉｎｔｈｅｐｉｔｕｉｔａｒｙｇｌａｎｄａｎｄｈａｓｂｅｅｎｓｈｏｗｎｔｏｐｌａｙａｎｅｓｓｅｎｔｉａｌｒｏｌｅｉｎｔｈｅｓｔｉｍｕｌａｔｉｏｎｏｆｓｏｍａｔｉｃｇｒｏｗｔｈａｎｄｄｅｖｅｌｏｐｍｅｎｔｉｎｖｅｒｔｅｂｒａｔｅ .Ｓｅｖｅｒａｌｓｔｕｄｉｅｓｗｅｒｅｃｏｎｄｕｃｔｅｄｏｎｔｈｅｇｒｏｗｔｈ ｐｒｏｍｏｔｉｎｇａｃｔｉｖａｔｉｏｎｏｆｖａｒｉｏｕｓｔｅｌｅｏｓｔＧＨｓｉｎｓａｌｍｏｎｉｄ… 相似文献
2.
Recombinant eel GH and yeast containing chinook salmon growth hormone (reGHand rcsGH) were incorporated into gelatin and sodium alginate (reGH-GS and rcsGH-GS) or polymer ma-trix (reGH-HP55) to protect the hormone from proteolytic cleavage in the stomach. The diets containin greGH-GS, rcsGH-GS, reGH-HP55 and free-reGH or uncoated-rcsGH were administered to red sea bream. Feeding of reGH-GS, reGH-HP55 and rcsGH-GS diets resulted in significant increases in body weight and fork length over those of controls. These results strongly suggest that gelatin and sodium algi-nate as well as polymer matrix protected the hormone from proteolytic enzymes in the gastrointestinal tract to allow the bioactive hormone to enter the circulation and eventually stimulato fish growth. 相似文献
3.
Effects of salinity, light and temperature on growth rates of two species of Gracilaria （Rhodophyta） 总被引：2，自引：0，他引：2
Effects of temperature, salinity and light intensity on growth rates of Gracilaria lichenoides and G. tenuistipitata var. liui Zhang et Xia were tested. Eight to ten levels of each factor were first tested separately. The best growth rate was obtained
under the conditions of 32°C, 30 and 240 μmol/(m2·s) for G. lichenoides, and 24°C, 20 and 200 μmol/(m2·s) for G. tenuistipitata, respectively. Then a uniform design was used to evaluate the optimal combinations of the three factors. The best conditions
for the highest daily specific growth rates (% increase in wet weight) are determined to be 31.30°C, 32.10, and 287.23 μmol/(m2·s) for G. lichenoides (16.26%/d), and 25.38°C, 21.10, and 229.07 μmol/(m2·s) for G. tenuistipitata (14.83%/d), respectively.
Supported by the 908 Special Program (908-02-04-07), the National Basic Research Program of China (973 Program, No. 2006CB400608),
and K. C. Wong Magna Fund in Ningbo University 相似文献
4.
A 4.34 liter two-stage air-lift photobioreactor incorporatingAnabaena variabilis ATCC29413 mutant PK84 was used to study H2 production. Results showed that H2 production increased with increasing light intensity from 47 μE/(m2·s) up to 190 μE/(m2·s), but that further increase of light intensity decreased the H2 production because of the inhibition due to the high pO2. The data also indicated that longer argon gas charge resulted in more H2 produced due to the increase of nitrogenase activities and heterocyst frequency, and that more than 1.3 L net H2 was produced from this computer controlled photobioreactor. 相似文献
5.
A filamentous clone of Polysiphonia urceolata was regenerated from segments cut from the fronds of gametophytes. Unlike wild thalli with short virgate branchlets, the clone was filamentous with few branches. Many transparent trichoblasts arose from pericentral cells during the induction culture, but these were seldom observed during normal growth. The trichoblasts were uniseriate, often colorless, and formed lobed rhizoids rapidly when they came into contact with solid substrates. In addition to morphological characteristics, the photosynthetic properties and growth conditions of the clone differed from those of the mother plant. Cross-gradient light and temperature culture experiments revealed that the most favorable conditions for culture of the filamentous clone were 22°C and 95-120 μE/(m2-s) light intensity. The photosynthetic light saturation value for filaments was approx. 100 μE/(m2-s), which is far lower than that of wild thalli. These results could be used to develop techniques for mass cultures of P. urceolata in photobioreactors for production of seed stock or bioactive products. 相似文献
6.
During a survey for marine microalgal resources, we isolated a rare marine euglenoid from the coastal waters of Qingdao, China in 2009, and established a pure culture. Electron microscopic and molecular phylogenetic (18S rDNA and 16S rDNA sequences) analyses revealed a close affinity with Eutreptiella gymnastica, a bloom-forming species. Different culture conditions were monitored to understand optimal E. gymnastica growth characteristics. The optimal growth conditions in a batch culture of this isolate were 20°C, 160 μmol photons/(m2×s) of white light, and a salinity of 10-31. Nutrient experiments demonstrated that growth increased dramatically with a phosphorus concentration greater than 72 μmol/L. Understanding the effect of culture conditions on E. gymnastica may help understanding the blooming mechanism of this alga in its natural environment. 相似文献
7.
8.
Effect of temperature and irradiance on growth and reproduction of Enteromorpha prolifera that bloomed offshore along the Qingdao coast in summer 2008, was studied. It was showed that E. prolifera propagated mainly asexually with specific growth rate （SGR） of 10.47 at 25℃/40 μmol m^-2s^-1. Under this condition, gametes with two flagellate formed and released in 5 days. At the beginning of the development, the unicell gamete divided into two cells with heteropolarity, and then the apical cell developed into thalli primordial cells, whereas the basal cell developed into rhizoid primordial cells. In 8-day culture, the monoplast gamete developed into juvenile germling of 240 μm in length. Unreleased gametes can develop directly within the alga body. E. prolifera could either reproduce through lateral branching or fragmenting except apomixis revealed by Microscopic observation. On aged tissue of E. prolifera, although the degraded pigments partially remained in faded algal filaments, numerous vegetative cells could still divide actively in the algal tissues. 相似文献
9.
The changes in arachidonic acid (AA) and fatty acids profiles along the growth curve ofParietochloris incisa, a coccoid snow green alga, were studied in a 2.8 cm light-path flat photobiorcactor, exposed to strong photon flux density
[PFD, 2400 μEmol/(m2·s)]. Sixteen fatty acids were identified by gas chromatography showing that AA was the dominant fatty acid (33%–41%) followed
by linoleic acid (17%–21%). AA content was closely investigated with respect to total fatty acids (TFA), ash free dry weight
(AFDW) of cell mass as well as total culture content. These parameters were influenced significantly in a similar manner by
culture growth phase, i.e., slightly decreasing in the lag period, gradually increasing in the logarithmic phase, becoming
maximal at the early stationary phase, starting to decrease at the late stationary phase, sharply dropping at the decline
phase. The increase in AA per culture volume during the logarithmic phase was not only associated with the increase in AFDW
but also connected with a corresponding increase in AA/TFA, TFA/AFDW as well as AA/AFDW. The sharp decrease in AA content
of the culture during the decline phase was mainly due to the decrease in AA/TFA, TFA/AFDW and AA/AFDW, although AFDW declined
only a small extent. Maximal AA concentration, obtained at the early stationary phase, was 900 mg/L culture volume, and the
average daily net increase of AA during 9 days logarithmic growth was 1.7 g/(m2·day). Therefore, harvesting prior to the decline phase in a batch culture, or at steady state in continuous culture mode
seems best for high AA production. The latter possibility was also further confirmed by continuous culture with 5 gradients
of harvesting rate.
Contribution No. 4138 from the Institute of Oceanology, Chinese Academy of Sciences.
Project 39970575 supported by NSFC and A/2786-2 supported by International Foundation for Sciences (IFS). 相似文献
10.
IAA 3-Indolylacetic acid, NAA a-Naphthylacetic acid and cytokinins in PESI culture medium were used in a study on the effects
of plant hormones on the growth of free-living conchocelis ofPorphyra yezoensis which showed that its growth in medium with cytokinins, IAA and NAA was more rapid than that in medium with non—phytohormones;
that the optimal concentrations for promoting growth were 10 μg/L for IAA and ZA (Zeatin), and 0.1 μg/L for BA 6-Benzyl amino
purine and KIN 6-Furfurylamino- purine. Mix use of NAA, IAA and cytokinins, NAA/ZA 1–1000/1 μg/L, NAA/BA 10/1-1000 μg/L, NAA/KIN
1/1-1000 μg/L promoted growth. IAA/ZA 0.1-1/0.1-1 μg/L; IAA/BA 0.1-1/0.1-10 μg/L IAA/KIN 1/0.1-1000 μg/L also promoted growth.
Contribution No. 2335 from the Institute of Oceanology, Chinese Academy of Sciences. Contribution No. 94 from the Experimental
Marine Biology Lab Qingdao, China.
The research was supported by a grant from the Chinese Academy of Sciences. 相似文献
11.
The effect of micronized frustules of fossil diatoms (DS) on tumor growth and survival of mice receiving an ip inoculations
of either a spontaneous lymphoblastic leukemia (LB) or P-388 lymphocytic leukemia (P-388) was investigated. A multi-dose study
was designed, mice receiving 1000, 100, 10 and 1 μg of a suspension of DS, either ip or sc on day 1, 4 and 9 after tumor inoculation.
With LB the best results were observed when DS were inoculated by the ip route, no effect being observed with the sc route.
The best therapeutic effect resulted with 1 μg DS: 33% of the mice were alive 24 days after tumor inoculation, when all the
controls had died. With P-388, only a temporary effect with the 10 μg DS dose was observed. In no case was it possible to
prolong survival. It is concluded that at low doses of DS therapeutic effect is greatest, while toxicity due to SiO2 is greatly reduced. 相似文献
12.
Experiments withLaminaria japonica were conducted in Meidao Bay and Pier Bay, Qingdao, China, Nitrogen-starved plants were fertilized intermittently with 7.1
mM NH4−N solution for 1 hour at 3 day intervals, after which chlorophyll a, fucoxanthin, chlorophyll c and β-carotene contents were
analyzed. Photosynthetic and growth rates of plants and nitrogen content of seawater were determined. their contents of chlorophyll
a, fucoxanthin, chlorophyll c and β-carotene were 1.65, 0.67, 0.33 and 0.06 mg.dm−2 respectively, or 2.46, 2.03, 1.86 and 1.81 times those of the controls. The ratio of fucoxanthin to chlorophyll a in nitrogen-enriched
plants was lower than that of the controls. Normal growth rate of the plants (2 cm·day−1 in length) were reached when the chlorophyll a content exceeded 0.1 mg·g−1 fresh wt., indicating that chlorophyll a content can serve as an indicator of normal growth.
Experimental results show that seawater is regarded as fertile for the normal growth ofLaminaria if the total inorganic nitrogen (including ammonium salt, nitrate and nitrite) content in seawater is about 2 μm, and infertile
if less than 1 μm, in which case fertilizer should be applied.
Contribution No. 1703 from the Institute of Oceanology, Academia Sinica 相似文献
13.
Light is an important parameter in algal culturing. In this work, the effects of different light qualities on growth of the marine diatom Chaetoceros gracilis are evaluated. The cells were cultured under light quantum flux density of 60 µmol photons/(m2·s) with nine light qualities: LED red light (LR), LED blue light (LB), LED red plus LED blue light (LR+LB), LED white light (LW), fluorescent white light (FW), and combinations of LW and FW lights with increased proportions of red or blue light (FW+LR, FW+LB, LW+LR, and LW+LB). Blue light promoted the growth of C. gracilis largely. Three light qualities, FW+LR, LW+LR, and LR, resulted in the lowest growth rate. Both chlorophyll and carotenoids reached the highest level under LB and the lowest level under LR among monochromatic light sources; however, increasing of the proportion of blue or red light in the white light induced an increase of the chlorophyll and carotenoid concentrations. Light absorption ability of microalgae was critical for the growth of these organisms, as we observed a positive correlation between the extinction coefficient at different wavelengths and the specific growth rate. These findings are of importance to improve the culturing of this alga in bioreactors. 相似文献
14.
ＩＮＴＲＯＤＵＣＴＩＯＮＭｏｒｅｔｈａｎａｄｅｃａｄｅｈａｓｐａｓｓｅｄｓｉｎｃｅｂａｃｔｅｒｉａｗａｓｒｅｃｏｇｎｉｚｅｄａｓｑｕａｎｔｉｔａｔｉｖｅｌｙｉｍｐｏｒｔａｎｔｃｏｎ ｓｕｍｅｒｓｏｆｏｒｇａｎｉｃｃａｒｂｏｎｉｎｍａｒｉｎｅｆｏｏｄｗｅｂｓａｎｄｍａｒｉｎｅｅｃｏｓｙｓｔｅｍｓ (Ｆｕｈｒｍａｎ ,1 992 ) .ＴｈｅｂａｓｉｃｉｎｆｏｒｍａｔｉｏｎｏｎｔｈｅｓｉｇｎｉｆｉｃａｎｃｅｏｆｔｈｅｍｉｃｒｏｂｉａｌｆｏｏｄｗｅｂｗａｓｐｒｅｓｅｎｔｅｄｂｙＰｏｍｅｒｏｙ ( 1 974 ) ,ｗｈｏｐｉｅ… 相似文献
15.
Acrochaete leptochaete (Huber) Nielsen (Chaetophoraceae, Chlorophyta) was isolated from the macroalgae Chaetomorpha collected from intertidal pools in Rongcheng, Shandong, China. 18S rDNA combined with ITS regions were used to ascertain the morphological identification of the isolated material. Based on the unialgal culture, asexual reproduction and growth characteristics of A. leptochaete were investigated over wide ranges of temperature and irradiance. Results revealed that asexual reproduction of A. leptochaete could be realized by biflagellate zoospores. The zoospores germinated directly to give self- replicating generations. Zoospore germination was bipolar. A temperature range from 13-21°C and a lower irradiance of 36 μmol/(m2·s) were most favorable for the growth of A. leptochaete. Thallus organization, an important taxonomic criterion for the genus Acrochaete, was affected markedly by temperature and irradiance. Our results extend the knowledge about the species’ general biology and its morphological plasticity. For classification and identification of a simple microphytic algae like A. leptochaete, which are traditionally placed in the class Chaetophoraceae, we propose that molecular tools associated with culture observations are applied. 相似文献
16.
Laboratory culture experiments showed that <100μ mol/L nitrate, amonium or mixture of amino acids promote the growth of the
red tide organismProrocentrum micans Ehrenb, but that >100μmol/L of ammonium, or mixture of glycine and glutamate was harmful to growth, and that orthophosphate
wasP. micans’ main phosphorous source in the ocean. Presence of 80μ mol/L EDTA, 0.5 to 1 μmol/L Fe3+, 1.0 to 20.0 μ mol/L Mn2+ 0.1 to 0.4 μmol/L Co2+ in the culture medium could improve the growth ofP. micans. Vitamin B1 promoted growth, but vitamin B12 and biotin did not. The estimated minimum cell quotas (q
o) for nitrogen and phosphorus being 0.74 pmole/cell and 0.045 pmole/cell show that phosphorus (more than nitrogen) limits
the growth ofP. micans in the study area.
This project was supported by the Natural Science Foundation of Zhejiang Province. 相似文献
17.
Abstact Age and growth characteristics of crimson sea bream Paragyrops edita Tanaka in Beibu Gulf were studied through bottom trawling and gillnet fleets fishing from July 2006 to December 2007. A total
number of 1155 individuals, ranging from 49 to 249 mm in standard length was examined. The age of the fish was determined
from sagittal otoliths. One year growth was made up of one translucent and one opaque zone. A maximum likelihood estimation
procedure was used to fit the Von Bertalanffy, Logistic and Gompertz growth functions to the length-at-age data. ARSS indicated
that there were no significant differences in growth between sexes in the three growth models (P>0.05), and the Von Bertalanffy growth function L
t
=292.8{1−exp [−0.167(t+1.116)]} was selected as the most appropriate growth model according to Akaike’s information criterion (AIC). 相似文献
18.
Requirement for dietary n−3 HUFA (n−3 highly unsaturated fatty acid) for growth and survival of black sea bream (Sparus macrocephalus) larvae was studied using rotifers andArtemia at various levels of n−3 HUFA. Five treatments with rotifers andArtemia differing in n−3 HUFA were prepared by enriching them with various oil emulsions. Results indicated that dietary n−3 HUFA
significantly influence fish n−3 HUFA levels and are essential for growth and survival of black sea bream larvae. The results
also indicated that the incorporation of n−3HUFA TG (triacylglycerols) into tissues of larval black sea bream was more effective
from natural fish oil in comparison with n−3 HUFA fatty acid ethyl esters from ethyl-esterified oil.
Project No. 39770588 supported by the NSFC. 相似文献
19.
In order to improve production and breed new broods of bay scallopArgopecten irradians irradians, different-colored orange, purple and white lines were established by two mating methods of self-fertilization and mass spawning
at a commercial scallop hatchery in spring, 2002. And then larval growth and survival of different lines was compared to test
whether there is a relationship between shell color and growth and survival at early developmental stage. Both growth and
survival have no significant differences among different experimental larvae of self-fertilization or mass spawning. Results
are as following in the order of orange, purple and white shell stock. For the self-fertilization, growth rates of larvae
were 6.174, 6.412, and 6.599 μm/d, respectively. Survival rates of larvae at Day 3 were 74.41%, 76.86%, and 82.05%; Day 6
were 49.14%, 65.63%, and 52.79%; and Day 9 were 25.06%, 20.80%, and 26.47%, respectively. For the mass spawning, the growth
rates were 7.836, 7.941, and 7.878 μm/d, respectively. Survival rates at Day 3 were 93.05%, 91.95%, and 92.50%; Day 6 were
79.17%, 78.05%, and 82.50%; and Day 9 were 34.72%, 36.67%, and 38.33%, respectively. The absence of any relationship between
shell color and growth and survival at the larval stage may be resulted from their common genetic basis.
This work was financially supported by Project of Scientific Innovation, Chinese Academy of Sciences (ZKCX2-211). 相似文献
20.
In laboratory conditions, effects of rearing temperature and stocking density were examined on hatching of fertilized egg
and growth of auricularia larvae of Apostichopus japonicus respectively. Data series like larval length and density, metamorphic time, and survival rate of the larvae were recorded.
Statistics showed that for A. japonicus, survival rate (from fertilized egg to late auricularia) decreased significantly with the increasing rearing temperature
(P<0.05). At different temperatures SGR was statistically significant as well (P<0.05) from day 1, and maximal SGR was found on day 9 at 24°C (159.26±3.28). This study clearly indicated that at low temperature
(<24°C), metamorphic rate was remarkably higher than at higher temperature (>26°C). Hatching rate was significantly different
between 0.2–5 ind./ml groups and 20–50 ind./ml groups. Rearing larvae at the higher density had the smaller maximal-length,
whereas needed longer time to complete metamorphosis. This study suggested that 21°C and 0.4 ind./ml can be used as the most
suitable rearing temperature and stocking density for large -scale artificial breeding of A. japonicus’s larvae. 相似文献