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1.
After discarding marine microorganisms from conchocelis of Porphyra yezoensis and Porphyra ha/tanens/s, their axenic cultures were obtained through treatment with antibiotics. Antibiotic disc tests were carried out to determine the effectiveness of each antibiotic in eliminating contaminating microorganisms. Five of 12 antibiotics tested were selected and used to produce the axenic cultures in this study, which showed that 200 μg/mL streptomycin, 250 μg/mL penicillin, 252 μg/mL kanamycin, 30 μg/mL neomycin, 200 μg/mL chloramphenicol were effective concentrations for eliminating microorganisms from conchocelis when antibiotics were added singly step by step; whereas simultaneous combination of 150 μg/mL streptomycin, 250 (or 350) μg/mL penicillin, 150 (or 250) μg/mL kanamycin, 70 μg/mL neomycin and 200 μg/mL chloramphenicol was also effective for producing the axenic cultures. However,it seemed that the treatments with antibiotics applied individually were more feasible than those with all antibiotics added at the same time. This may be due to the combined inhibiting effect of antibiotics on the growth and development of conchocelis. 相似文献
2.
This study showed lethal concentrations (LC) of copper forPeneaus penicillatus at various stages of its life cycle were 1000 μg/L for nauplii, 1000 μg/L for Zoea I, 2000 vg/L for Zoea II, 2500 μg/L for
Zoea III, 3000 μg/L for Mysis I, II and III and that for almost 100% mortality for postlarvae was 3000 μg/L. For cadmium LC
were 100 μg/L for nauplii, 500 μg/L for Zoea I, 1000 μg/L for Zoea II, 2000 μg/L for Zoea III, 2500 μg/L for Mysis I and 3500
μg/L for Mysis II, III and postlarvae. For mixture of both metals, LC were 400 μg/L for nauplii, 1000 μg/L for Zoea I, 2000
μg/L for Zoea II and 3000 μg/L for Mysis I, II, III and post larvae. 相似文献
3.
吕庆芳 《广东海洋大学学报》2003,23(4):57-61
以粉蕉的吸芽为试材 ,进行了离体快速繁殖研究 ,结果表明 :以改良MS +BA4 .0mg/L(单位下同 ) +AD5.0 +NAA0 .1作诱导培养基 ,外植体褐变率低 ,芽的诱导率高 ,且芽的生长良好 ;以改良MS +BA3- 6 +NAA0 .1为继代培养基 ,可以获得较高的不定芽增殖率 ,在增殖过程中 ,给予一定的低温处理和变化调整BA的用量 ,可以保持较高的增殖率 (2 .6倍以上 ) ,而且芽的长势好 ;生根培养以 1/ 2MS +IBA1.0 +NAA0 .2 +AC1为宜。 相似文献
4.
Results of neutron activation analysis (NAA) ofSargassum thumbergii samples collected from the Qingdao Taipingjiao coast on March 20, 1996 showed that (1)Sargassum thunbergii can to some extent accumulate potassium and calcium (the accumulation coefficient was 10); (2) it can accumulate almost all
trace elements, especially iron, managanese and zinc; (3) it can accumulate strontium, aluminium thorium, and rare earth elements.
Contribution No.3308 from the Institute of Oceanology, Chinese Academy of Sciences.
The study supported by the National Ninth-Five-Year Key Project “96-916-04-01”. 相似文献
5.
Tetraploid induction was carried out by inhibiting mitosis I in fertilized eggs ofChlamys farreri. Mitosis I was blocked with cold shock (5–7°C), Cytochalasin B (0.75 mg/L) and 6-dimethylaminopurine (6-DMAP) (60–75 mg/L)
when 60% fertilized eggs released polar body II at 20°C. At 4-cells embryo stage, the ploidy was determined by counting chromosome
number. In control groups, most embryos were diploids (72.22%) and aneuploids (24.78%). In Cytochalasin B, cold shock and
6-DMAP treated groups, tetraploids were respectively 10.51%, 4.08%, and 13.34%; aneuploids were 43.10%, 35.93% and 29.16%,
and triploids were 7.84%, 8.52% and 18.33%. At D-larva stage, ploidy was determined by flow cytometry (FCM). The ploidy analysis
of day 2 larvae showed diploids in control group and also in three treated groups. Juvenile scallops (0.2–0.3cm) which were
harvested in two control groups and two CB treated groups were all diploids through checking ploidy individually by FCM.
Contribution No. 3648 from the Institute of Oceanology, Chinese Academy of Sciences.
Contribution No. 238 from the Experimental Marine Biology Laboratory, Chinese Academy of Sciences.
Project funded by grant (No. 819-01-07) from the Chinese Science and Technology Ministry and also by “Hundred Talents Plan”
grant from the Chinese Academy of Sciences. 相似文献
6.
Dunaliella salina, a halotolerant unicellular green alga, can accumulate a large amount of β-carotene under certain environmental conditions.
The isomers of β-carotene extracted fromD. salina cultured in medium with different nitrate and phosphate concentrations were analysed by HPLC with Alox-T alumina column.
At least six isomers were found in different proportions depending on the culture media's nitrate and/or phosphate concentrations.
Nitrate and/or phosphate deficiency was conducive to the accumulation of total cis isomers but not of all trans isomer. It
is suggested that 1 mmol/L KNO3 and 0.1 mmol/L KH2 PO4 are favourable for accumulation of total cis β-carotene.
Contribution No. 2090 from the Institute of Oceanology, Chinese Academy of Sciences.
This project was supported by the National Natural Science Foundation of China, Grant No. 38970587. 相似文献
7.
Laboratory culture experiments showed that <100μ mol/L nitrate, amonium or mixture of amino acids promote the growth of the
red tide organismProrocentrum micans Ehrenb, but that >100μmol/L of ammonium, or mixture of glycine and glutamate was harmful to growth, and that orthophosphate
wasP. micans’ main phosphorous source in the ocean. Presence of 80μ mol/L EDTA, 0.5 to 1 μmol/L Fe3+, 1.0 to 20.0 μ mol/L Mn2+ 0.1 to 0.4 μmol/L Co2+ in the culture medium could improve the growth ofP. micans. Vitamin B1 promoted growth, but vitamin B12 and biotin did not. The estimated minimum cell quotas (q
o) for nitrogen and phosphorus being 0.74 pmole/cell and 0.045 pmole/cell show that phosphorus (more than nitrogen) limits
the growth ofP. micans in the study area.
This project was supported by the Natural Science Foundation of Zhejiang Province. 相似文献
8.
1INTRODUCTIONInrecentyearsglobalclimatechangehasbeenanissueofbothpublicandscientificconcern.Oceanscover71 %ofearthandplayamajorroleinglobalclimatechange.Phosphorus,asacriticalelement,regulatespri maryproductioninaquaticecosystems.Therefore,thefunctionofphosphorusinoceansystemhasreceivedin creasingscientificinterest(Songetal.,2 0 0 3 ) .PP (particulatephosphorus)isanimportantforminphosphorustransferinseawater(Song,2 0 0 1 ) .Pro portionofPPintotalphosphorus(TP)inseawatervar iesfrom 1 0 … 相似文献
9.
The iodination efficiency of salmon GH (sGH) was 38.82%,using a modification of the chloramine-T method. The specific activity of the ^125I-sGH was about 40 μCi/μg protein. The results of binding assay showed a single class of high affinity and low-capacity binding site in flounder liver. Long-term administration with exogenous GH can induce the up-regulation of hepatic GH receptor in total binding capacity though there was no significant difference of association constant among any groups. Con-sidering that there was no significant difference in capacity of free binding sites of livers from control and experimental fish, this result also indicated that the liver from experimental fish, compared to that from control fish, had more occupied binding sites. 相似文献
10.
Effects of reduced salinity on the oxygen consumption rate (OCR) and the ammonia-N excretion rate (AER) of scallopChlamys farreri (3.2–5.9 cm in shell height, 0.147–1.635 g in soft tissue dry weight) were studied in laboratory from March 21, 1997 to April
16, 1997. Under the controlled conditions of reduced salinity from 31.5 to 15.0 and ambient temperature 17°C and 23°C, the
concentrations of dissolved oxygen and ammonia-N were determined by the Winkle method and the hypobromite method, respectively.
Results showed that with controlled reduced salinity, the mean values of the OCR were 2.17 mg/(g.h) at 17°C, and 2.86 mg/(g.h)
at 23°C and that the mean values of the AER were 178.0 μg/(g.h) at 17°C and 147.0 μg/(g.h) at 23°C. The OCR and the AER decreased
with reducing salinity from 31.5 to 15.0 both at 17°C and 23°C. The effects of reduced salinity on the OCR and the AER of
scallopC. farreri could be represented by the allometric equation and the exponential equation, respectively.
Contribution No. 3295 from the Institute of Oceanology, Chinese Academy of Sciences.
Project supported by the National Ministry of Science and Technology of China and by the Chinese Academy of Sciences, Grant
No. 96-922-02-04 and KZ951-A1-102-02. 相似文献
11.
Thirty-two 63.0–134.4 mm standard length specimens collected during 1954–1990 from the coastal waters of the South China Sea
and the continental coast of the Taiwan Strait were identified as a new species,Pampus minor Liuet Li, sp. nov.
Contribution No. 3329 from the Institute of Oceanology, Chinese Academy of Sciences.
Study supported by Chinese Academy of Sciences Special Grant for Biota Research. 相似文献
12.
沈志良 《中国海洋湖沼学报》1996,14(4):303-307
The distributions of SiO_3-Si, PO_4-P, NO_3-N and NO_2-N concentrations in the seawater, iceand snow from 82°45′21″W, 88°01′20″N to the North Pole are reported and the exchange of thenutrients among the seawater, ice and snow are discussed. The average concentrations of the nutrients inthe seawater near the North Pole were 4.9±2.4μmol/L for SiO_3-Si; 0.60±0.10μmol/L for PO_4-P,3.4±1.7μmol/L for NO_3-N and 0.13±0.04μmol/L for NO_2-N. The nutrient concentrations inthe ice and snow in the North Pole were 0.20 and 0.65μmol/L for SiO_3-Si; 0.22 and 0.25μmol/L forPO_4-P; 1.6 and 5.8μmol/L for NO_3-N, 0.19 and 0.15μmol/L for NO_2-N. 相似文献
13.
Effects of temperature on oxygen consumption rate (OCR) and ammonia—N excretion rate of scallopChlamys farreri (1.7–6.2 cm in shell height) were studied in laboratory from Dec. 30,1996 to Jan. 28,1997. Under the controlled conditions
of ambient water temperature 10–31°C and salinity 32, the concentrations of dissolved oxygen and ammonia—N were determined
by the Winkle method and the hypobromite method, respectively. Results showed that the OCR ranged from 1.20 mg/g (DW) · h
to 5.76 mg/g (DW) · h. The OCR increased with temperature from 10°C to 23°C, but at 28°C the OCR of mature individuals decreased,
and that of different size scallops reduced at 31°C. The ammonia—N excretion rate ranged from 113.03 μg NH4-N/g (DW) · h to 486.63 μg NH4-N/g (DW) · h, and increased with temperature from 10°C to 31°C.
Contribution No.3180 from the Institute of Oceanology, Chinese Academy of Sciences. This work was supported by the National
Commission of Science and Technology of China, Grant No.96-922-02-04. 相似文献
14.
The changes in arachidonic acid (AA) and fatty acids profiles along the growth curve ofParietochloris incisa, a coccoid snow green alga, were studied in a 2.8 cm light-path flat photobiorcactor, exposed to strong photon flux density
[PFD, 2400 μEmol/(m2·s)]. Sixteen fatty acids were identified by gas chromatography showing that AA was the dominant fatty acid (33%–41%) followed
by linoleic acid (17%–21%). AA content was closely investigated with respect to total fatty acids (TFA), ash free dry weight
(AFDW) of cell mass as well as total culture content. These parameters were influenced significantly in a similar manner by
culture growth phase, i.e., slightly decreasing in the lag period, gradually increasing in the logarithmic phase, becoming
maximal at the early stationary phase, starting to decrease at the late stationary phase, sharply dropping at the decline
phase. The increase in AA per culture volume during the logarithmic phase was not only associated with the increase in AFDW
but also connected with a corresponding increase in AA/TFA, TFA/AFDW as well as AA/AFDW. The sharp decrease in AA content
of the culture during the decline phase was mainly due to the decrease in AA/TFA, TFA/AFDW and AA/AFDW, although AFDW declined
only a small extent. Maximal AA concentration, obtained at the early stationary phase, was 900 mg/L culture volume, and the
average daily net increase of AA during 9 days logarithmic growth was 1.7 g/(m2·day). Therefore, harvesting prior to the decline phase in a batch culture, or at steady state in continuous culture mode
seems best for high AA production. The latter possibility was also further confirmed by continuous culture with 5 gradients
of harvesting rate.
Contribution No. 4138 from the Institute of Oceanology, Chinese Academy of Sciences.
Project 39970575 supported by NSFC and A/2786-2 supported by International Foundation for Sciences (IFS). 相似文献
15.
The ultrastructure of hemocytes ofPenaeus vannamei was examined by transmission electron microscope. Three types of hemocytes were identified: hyaline cell, small-granule cell
and large-granule cell. Hyaline cells are the smallest of the hemocytes, lack electron-dense granules, have average size of
11.11 μm (SE±0.50,n=10)×6.96μm (SE±0.55,n=10), comprise 25.8% (SE±2.87,n=4×10) of the hemocytes; small granule cells are the most abundant type of hemocytes, comprise 58.1% (SE±3.40,n=4×10) of the hemocytes, contain small electron—dense granules, have average size of 10.78 μm (SE±0.60,n=10)×8.63 μm (SE±0.44,n=10); and large-granule cells comprise 16.1% (SE±2.55,n=4×10) of the hemocytes, are filled with large electron dense granules, have average size of 12.51 μm (SE±0.63,n=10)×8.99μm (SE±0.71,n=10).
This research was supported by the National Climbing Project B, PDB6-6-3.
Contribution No. 3448 from the Institute of Oceanology, Chinese Academy of Sciences. 相似文献
16.
DYNAMIC CHANGES OF INORGANIC NITROGEN AND ASTAXANTI—IIN ACCUMULATION IN HAEMATOCOCCUS PLUVIALIS 总被引：1，自引：1，他引：0
This study on dynamic changes of culture color,astaxanthin and chlorophylls,inorganic N including N-NO3^-,N-NO2^- and N-NH4^ in batch culture of Haematococcus pluvialis exposed to different additive nitrate concentration showed(1)ast/chl ratio was over 0.8 for brown and red algae,but was usually less than 0.5 for green and yellow algae;(2)N-NO3^-,in general,was unstable and decreased,except for a small unexpected increase in nitrate enriched treatment groups;(3)measurable amounts of N-NO2^- and N-NH4^ were observed respectively with three change modes although no extemal nitrite and ammonia were added into the culture;(4)a non-linear correlation between ast/chl ratio(or color)changes and the levels of N-NO3^-,N-NO2^-,N-NH4^ in H.pluvialis culture;(5)up and down variation of the ast/chl ratio occurred simultaneously with a perceptible color change from yellow to brown(or red)when N-NO3^-,N-NO2^- and N-NH4^ fluctuated around 30,5,5μmol/L respectively;(6)existence of three dynamic modes of N-NO3^-,N-NO2^- and N-NH4^ changes,obviously associated with initial extemal nitrate;(7)the key level of total inorganic N concentration regulating the above physiological changes during indoor cultivation was about 50 μmol/L;and(8)0.5-10mmol/L of nitrate was theoretically conducive to cell growth in batch culture. 相似文献
17.
A combined numerical model of wind, wave, tide, and storm surges was built on the basis of the “wind field model in limited
sea surface areas”. When used to forecast the sea surface wind, wave height and water level, it can describe them very well.
Contribution No. 4108 from the Institute of Oceanology, Chinese Academy of Sciences.
This work supported by Stress Project (KZ952-S1-420), Chinese Academy of Sciences; 863 Project (863-818-06-05), and (863-818-Q-07) 相似文献
18.
Experiments on salimity tolerance of embryos and larvae of the southern bay scallopArgopecten irradians concentricus Say under controlled temperature of 28°C showed that although the eggs were fertilized and cleaved at salinity 18.1–37.4,
they could further develop into normal D-shaped larvae only at salinity 23.4–36.1, while the trochophore larvae could develop
into normal D—shaped larvae at salinity 22.1–39.9. For 72 hours, the larvae could tolerate salinity of 18.4–43.9, the salinity
favoring growth was 19.8–42.4; the suitable range was 21–36, and the optimum at about 28. They metamorphosed at salinity 18.9–43.5,
optimally at 24–32.
Contribution No. 3301 from the Institute of Oceanology, Chinese Academy of Sciences.
The research was supported by the National Climbing Project B(PDB6-3-2). 相似文献
19.
When cultured under certain environmental conditions (25°C, light intensity 80 μmol/m2·s, LD 12/12, in enriched seawater medium with 7×10−4 mol/L NO3−N, 1.56×10−4 mol/L, PO4−P and supplements of other elements like Mn, Fe, I, etc.), male and female gametophytes ofU. pinnatifida kept growing vegetatively and propagated fast at average daily fresh weight increase rate of about 20%. The empirical formulaG
m=G
o·3m was established to estimate the output of vegetative gametophytes. Vigorous vegetative gametophyte cells began to form reproductive
structures (oogonium and spermatangium, when the temperature was lower than 25°C and other environmental factors were kept
optimal. The sufficient supply of gametophyte cells provided enough seeds for raisingUndaria sporelings on production scale. Controlled cross-breeding experiments using selected male and female gametophyte clones which
increase their cell number by mitosis instead of meiosis were also carried out in vitro. Juvenile sporophytes from the cross-breeding
had almost the same length and width increase rates as those of the control. The fact that vegetative gametophytes can be
purposely selected, propagated quickly, cross-bred, with the resulting sporophytes having almost the same characteristics
leads to a new way to select desiredUndaria strains for long time use without losing the desired economic characteristics.
Contribution No. 2696 from the Institute of Oceanology, Chinese Academy of Sciences.
Supported by National Natural Science Foundation of China. 相似文献
20.
CHARACTERISTICS OF THE OXYGEN REDUCTIONIN ATMOSPHERIC CORROSION 总被引：1，自引：0，他引：1
ImODUcrlONArnaorandnowincreasinglyimPoftantprobleminthemanyyearsinvestigationsofabosphericcormsionofmetaIsandalloyisthatthecormsionrmctionunderathineleCtrolytelayerchangessigwhcanhywithdrying.Tornashov(l966)reportedthatthecorm-sionrateisinfluentalbytheelamlytelayerthwhnessandiscontrolledbythedtheion-lndtingcurrentofoxygenreduCtionreig.l)whentheel~lytefilmisthickerthanthediffUSionlayerthickness.ThereforetherateisnotaffededbytheelCCtrolyteFilmthwh-nessforthecaseofaverythickFilm,e.g.regionI… 相似文献