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1.
Calculation and comparative study of the chlorophyll fluorescence ratio F685/F735 in brown algae (Laminaria japonica, Underia pinnatifida and Padina crassa) excited by blue and green light showed that the fluorescence ratios were higher when the algae were excited by blue light (440 nm), but reduced obviously under green light (540 nm) excitation. The values also reduced under dehydration but could recover during rehydration if the stress was not serious. The variation of the fluorescence ratio under dehydration was mainly because changes in fluorescence emission at 735 nm were always sharper than those at 685 nm. The ratio was sensitive to stress and has potential as a stress indicator in phycological research. Measurement of the fluorescence excitation spectra showed that the only peak at 540 hm changed apparently during dehydration. It meant that the function of the Chl a /Fucoxanthin protein complex for energy transfer was easily inhibited by water stress. However, no variation of the ratio was foun 相似文献
2.
The chorophyll fluorescence ratio F685/F735 in vivo can be a useful indicator for stress detection inhigher plants and seaweed. DCMU [3 - (3, 4 - dichlorophenyl ) - 1, 1 - dimethylurea] treatment influencesthis ratio. Th effects of DCMU on F685/F735 of marine red, brown and green algae under excitationlight of different wavelengths were investigted. In the brown algae, Laminaria japonica and Undariapinnatifida,DCMU did not increase this ratio under blue light excitation but increased the ratio slightlyunder excitation by green light. For the red algea, Halymenia sinensis, DCMU increased the ratiomarkedly under both blue and green ligh excitation. The percentage increase could reach 50% (undergreen light excitation) and was due to unequal enhancement at the two emission maxima by DCMU. Afraction of chlorophyll which contributed to fluorescence in the 735 nm region was less sensitive toDCMU and was likely from photosystem I of red algae. In the green alga, Ulva pertusa, DCMU causeda slight increase in F685 相似文献
3.
Nutrient limitation is known to inhibit growth and metabolism and to alter elemental stoichiometric ratios in phytoplankton. In this study, physiological changes in Thalassirosira weissflogii were measured under different dissolved inorganic phosphate (DIP) regimes in semi-continuous cultures to revisit the utility of the Redfield ratio for assessing nutrient limitation. The results showed that cell size increased with decreasing DIP availability. In the P-depleted treatment (f/2-P) the cell size was 1.48 times larger than that in the P-limited (f/100) treatment and 2.67 times larger than that in the P-saturated treatment (f/2 and f/10). The fucoxanthin to chlorophyll a ratio (Fuco/chl a) was relatively stable (about 0.3) in P-saturated cultures and was 10 times higher than that in P-limited and P-depleted cultures. During the experimental period, the photosynthetic efficiency index, ?F/Fm′, was relatively stable at ~0.50 in the P-saturated cultures, but quickly declined with decreasing DIP availability. Although cellular P content showed a significant difference between the P-saturated culture (1.6 pg/cell) and the P-limited culture (0.7 pg/cell), the N/P ratio in T. weissflogii did not show a trend with DIP availability and fluctuated slightly around 25. Our results suggest that cell division in T. weissflogii is not strictly size-gated but is probably regulated by a biochemical, and hence, an elemental stoichiometric ratio threshold, and that deviation of the cellular N/P ratio from the Redfield ratio is not a reliable indicator of algal nutrient stress. 相似文献
4.
In this paper, we investigated the effects of temperature, oxygen, antioxidants, and corn germ oil on the stability of astaxanthin from Haematococcus pluvialis under different storage conditions, and changes in the composition of astaxanthin esters during storage using high performance liquid chromatography and spectrophotometry. Oxygen and high temperatures （22-25~C） significantly reduced the stability of astaxanthin esters. Corn germ oil and antioxidants （ascorbic acid and vitamin E） failed to protect astaxanthin from oxidation, and actually significantly increased the instability of astaxanthin. A change in the relative composition of astaxanthin esters was observed after 96 weeks of long-term storage. During storage, the relative amounts of free astaxanthin and astaxanthin monoesters declined, while the relative amount of astaxanthin diesters increased. Thus, the ratio of astaxanthin diester to monoester increased, and this ratio could be used to indicate if astaxanthin esters have been properly preserved. If the ratio is greater than 0.2, it suggests that the decrease in astaxanthin content could be higher than 20%. Our results show that storing algal powder from H. pluvialis or other natural astaxanthin products under vacuum and in the dark below 4~C is the most economical and applicable storage method for the large-scale production of astaxanthin from H. pluvialis. This storage method can produce an astaxanthin preservation rate of at least 80% after 96 weeks of storage. 相似文献
5.
We used standard periphyton samplers to examine the colonization pattern of periphytic algae on artificial substrates (glass slides) in Lam Tsuen River, Hong Kong, in dry (winter) and wet (summer) seasons. In each season, six replicated slides were retrieved randomly and replaced by new slides at weekly intervals over a period of 6 weeks. We thus obtained two batches of slides, both with a series of different exposure times (1 to 6 weeks): one batch was set up at the same time (start of the sampling) and the other was harvested at the same time (end of the sampling). Changes in taxonomic composition, species diversity (Shannon-Wiener diversity index), standing crop (in terms of cell density and cell biovolume), and abundance of the abundant algal species were monitored and compared between the two batches of slides. The succession patterns of the periphytic algae were similar between the two batches in each season, while more remarkable differences were observed between the two seasons, suggesting that either batch would be suitable for a colonization study of periphytic algae. The cell density was dominated by diatoms in both seasons, while the cell biovolume was dominated by diatoms in winter and by green algae in summer. The cell density and biovolume attributed to blue green algae was relatively small. Most of the diatom species exhibited similar colonization patterns throughout the experiment, while green algae showed different succession patterns in different seasons or sampling methods, indicating that diatoms are better bio-indicators than green algae for a periphyton colonization study. In general, the diversity indices and the standing crops reached their maximums at around week 4, and they were higher in summer than in winter. 相似文献
6.
This study on the cytological changes of the body wall ,aspiratory intestine and crissal bursa in Urechis unicincuts by light microscope(LM) and transmission electron microscope(TEM) showed that the difference between the body wall and the natural environment rich in H2S was not obvious,that the wall color of the aspiratory intestine in H2S rich environment changed from normal semitransparency to dark brown.that its epithelia were disassembled and the electron density of its cytoplasm matrix was lower;and that in H2S rich environment many basoplhilic granules occurred in the epithelia of the crissal bursa.Granules with single membrane and myelinefingure were found with TEM. 相似文献
7.
This paper reports results of study on the contents of proteins, amino acids, polysaccharose and uronic acids in 30 species of macroalgae from Shicao, Heishijiao, Shimiao, and Xiaofujiazhuang in the vicinity of Dalian City, N.E.China. The results showed that the protein contents of the 30 algae from highest (112.55μg/ml) to the lowest (0.24 p.g/ml) was in the descending order of Dictyopteris ndalata, Gelidium vagum,Gymnogongrus japonican, Ectocarpus confervoides, Tinocladia crassa, Sargassum thunberii. In general, the protein content in red algae was higher than that in brown algae. The content of free ami no acids showed nosignificent differences from 7.44μg/ml-4.96μg/ml in all these algae, in the descending order of Gymnogongrusrus japonican, Sargassum confusum, Undoria pinnatifida, Laminaria japonica and Ectocarpus confervoides. The content of polysaccharose varied from 168.2 gg/ml-22.15 p.g/ml in the descending order of Symphyocladiaia tiuscula, Scytosiphon lomentarius, Desmarestia viridis., Tinocladia crassa, Gracilaria asiatica and Porphyra yezoensis. The content of uronic acids is from 196.24μg/ml-20.77μg/ml in the descending order of Ulva lactuca,Symphyoclaldia latiuscula, Scytosiphon lomentarius, Ceramimum kondoi, Gracilaria vemucosa and Porphyra yezoensis. The fatty acids in 30 species of algae belong to Rhodophyta, Chlorophyta and Phaeophyta. Most phaeophytes have many (4-12) types of fatty acids. 相似文献
8.
The bivalve Ruditapes philippinarum is not only one of the main mollusc species in the west Pacific Ocean, but also one of the main species for aquaculture in China. Knowledge of growth of the R. philippinarum shell will improve our understanding of the shell as an environmental archive. It is also useful for the aquaculture of R. philippinarum. In this research, a hanging box culture method was introduced in the culture of R. philippinarum. The bivalves were cultured for 126 days, from March 31 to August 3, 2002. The average growth rates of shell width, height, and thickness were 0.069, 0.046, and 0.032 mm/d, respectively. The mean increase of average individual wet mass was 0.028 g/d. The largest growth rates of both shell and average individual wet mass occurred in June, indicating that water temperature and bivalve reproduction were 2 important factors. The shell morphology underwent significant changes with shell growth. The ratio of height to thickness (value of B/C) shifted 1.58 in the first 60 days to 1.54 in the last 30 days, which was resulted from the change in major shell growth direction. Periodic changes in the B/C ratio led to corrugated shell form, which could be used to determine the age of the shell. 相似文献
9.
10.
In this study, we evaluated the chemical property and antioxidant activity of fucoidans isolated from brown algae, Laminaria japonica(LJF), Lessonia nigrescens(LNF), Lessonia trabeculata(LTF), Ascophyllum mackaii(AMF), and Ecklonia maxima(EMF). LJF was less in sulfate content(14.16%) and more in galactose and mannose content(1.08 and 0.68) than the documented early. EMF contained 20%–30% of sulfate and fucose, 0.97 in molar ratio which was lower than that of sulfate to other four fucoidans(1.21–1.41). AMF(162 kDa) and EMF(150 kDa) were the first two largest in molecular weight, which were followed by LJP(126 kDa), LNF(113 kDa) and LTF(105 kDa). The fucoidans isolated these algae showed a wide range of antioxidant activity in vitro. It was found that the reducing power of the isolated fucoidans was positively correlated with their sulfate content and molecular weight. In addition, LNF and LTF at low concentrations exhibited high superoxide and hydroxyl radical scavenging activity. This demonstrated that low molecular weight fucoidans may perform a high antioxidant activity. 相似文献
11.
Effects of salinity, pH, nitrogenous and phosphate nutrients on the growth rate of four species of benthic diatoms were studied
by using the method of in situ optical density measurement. The optimal culture conditions for the four species of diatoms
are as follows: forAmphora coffeaeformis, s 35; pH 8.5; NO3
−−N 1.54, NH4
+−N, 7.0; CO (NH2)2−N, 2.5 (mg/L); PO4
3−−P 1.13 (mg/L); forCocconeis scutellum varparva, s 30; pH 8.5; NO
3
−
−N 3.08, NH
4
+
−N 3.5, CO(NH2)2−N 5.0 (mg/L); pO
4
3−
−P 0.283 (mg/L); forNavicula corymbosa s 25; pH 8.0; NO
3
−
−N 1.54, NH
4
+
−N 3.5 CO(NH2)2−N 5.0 (mg/L); PO
4
3−
−P 0.565 (mg/L); forNavicula mollis. s 25; pH 8.0; NO
3
−
−N 1.54, NH
4
+
−N 1.75, CO(NH2)2−N 1.25 (mg/L); PO
4
3−
−P 0.141 (mg/L).
Part of the results of this paper were exchanged in the Fourth Asian Aquaculture Forum. Oct. 16–20. 1995. Beijing, P. R. China. 相似文献
12.
LIUDongyan AmyPickering SUNJun 《中国海洋大学学报(英文版)》2004,3(1):75-79
1 Introduction Inrecentyears ,eutrophicationhasbecomeaworld wideenvironmentalprobleminthecoastalarea.Aqua culturebecomesaseriouspollutionsourcelikesewagedischarge .Wasteproductsfromfishfarmsconsistma inlyofnitrogen ,phosphorusandcarbondioxide .Inpen bas… 相似文献
13.
Hamadoun BOKAR 《中国地理科学(英文版)》2004,14(1):63-70
1INTRODUCTIONGroundwater in Changchun City assures about 45% of total water supply. Drinking water supply of Chang-chun City was mostly served by surface water from Shitoukoumen and Xinlicheng reservoirs located in the east and south of the city (ZHANG, 1993). However, with the development of urban construction the ground-water especially from deep boreholes is also used for drinking purpose in suburban areas. The urbanization process in Changchun City threatens the groundwater quali… 相似文献
14.
The process-oriented model Forest-DNDC describing biogeochemical cycling of C and N and GHGs （greenhouse gases） fluxes （CO2, NO and N2O） in forest ecosystems was applied to simulate carbon sequestration and GHGs emissions in Abies fabric forest of the Gongga Mountains at southeastern edge of the Tibetan Plateau. The results indicated that the simulated gross primary production （GPP） of Abies fabric forest was strongly affected by temperature. The annual total GPP was 24,245.3 kg C ha^-1 yr^-1 for 2005 and 26,318.8 kg C ha^-1 yr^-1 for 2006, respectively. The annual total net primary production （NPP） was 5,935.5 and 4,882.2 kg C ha^-1 yr^-1 for 2005 and 2006, and the annual total net ecosystem production （NEP） was 4,815.4 and 3,512.8 kg C ha^-1 yr^-1 for 2005 and 2006, respectively. The simulated seasonal variation in CO2 emissions generally followed the seasonal variations in temperature and precipitation. The annual total CO2 emissions were 3,109.0 and 4,821.0 kg C ha^-1 yr^-1 for 2005 and 2006, the simulated annual total N2O emissions from forest soil were 1.47 and 1.36 kg N ha^-1 yr^-1 for 2005 and 2006, and the annual total NO emissions were 0.09 and o.12 kg N ha^-1 yr^-1 for 2005 and 2006, respectively. 相似文献
15.
Jiaozhou Bay data collected from May 1991 to February 1994, in 12 seasonal investigations, and provided the authors by the Ecological Station of Jiaozhou Bay, were analyzed to determine the spatiotemporal variations in temperature, light, nutrients (NO3^--N, NO2^--N, NH4^ -N, SIO3^2--Si, PO4^3--P), phytoplankton, and primary production in Jiaozhou Bay. The results indicated that only silicate correlated well in time and space with, and had important effects on, the characteristics, dynamic cycles and trends of, primary production in Jiaozhou Bay. The authors developed a corresponding dynamic model of primary production and silicate and water temperature. Eq. ( 1 ) of the model shows that the primary production variation is controlled by the nutrient Si and affected by water temperature; that the main factor controlling the primary production is Si; that water temperature affects the composition of the structure of phytoplankton assemblage; that the different populations of the phytoplankton assemblage occupy different ecological niches for C, the apparent ratio of conversion of silicate in seawater into phytoplankton biomas and D, the coefficient of water temperature‘s effect on phytoplankton biomass. The authors researched the silicon source of Jiaozhou Bay, the biogeochemical sediment process of the silicon, the phytoplankton predominant species and the phytoplankton structure. The authors considered silicate a limiting factor of primary production in Jiaozhou Bay, whose decreasing concentration of silicate from terrestrial source is supposedly due to dilution by current and uptake by phytoplankton; quantified the silicate assimilated by phytoplankton, the intrinsic ratio of conversion of silicon into phytoplankton biomass, the proportion of silicate uptaken by phytoplankton and diluted by current; and found that the primary production of the phytoplankton is determined by the quantity of the silicate assimilated by them. The phenomenon of apparently high plant-nutrient concentTations but low phytoplankton biomass in some waters is reasonably explained in this paper. 相似文献
16.
The effects of several nutritional factors on the growth and eicosapentaenoic acid (EPA) production of diatom Nitzschia laevis were studied. 4 LDM (quadrupled concentration of the nutrient salt) was the optimal concentration of nutrient salt for the
growth and EPA production of N. laevis. The growth of N. laevis was inhibited when the glucose concentration was either lower than 10 gL−1 or higher than 15 gL−1. Both sodium nitrate and urea were good nitrogen sources for the growth and EPA production, while ammonium chloride seriously
decreased the dry cell weight (DW) and the EPA content. Silicate seriously influenced the growth of N. laevis. The maximum DW of 2.34 gL−1 was obtained in the presence of 150 mgL−1 Na2SiO3·9H2O. The EPA content remained almost the same when the silicate concentration was lower than 150 mgL−1; however, higher silicate concentrations resulted in a steady decrease of EPA content. Low medium salinity (⩽29) did not
seem to influence the DW of N. laevis, and high salinity resulted in a decrease of DW. The highest EPA content (4.08%) and yield (110 mgL−1) were observed at the salinity of 36 and 29, respectively. 相似文献
17.
The effects of environmental Na+/K+ ratio on the gill ion-transport enzyme activity, plasma osmolality and growth of Cynoglossus semilaevis juveniles were investigated. The results showed that, plasma osmolality was similar among flounder adapted to different Na+/K+ ratios of saline groundwaters (P>0.05), while the growth, gill Na+, K+-ATPase and HCO3
−-ATPase activities were affected by Na+/K+ ratio significantly (P<0.05). The gill Na+, K+-ATPase activity reached its maximum on day 3, then decreased gradually from day 3 to day 9 and remained constant from day
9 to day 15. The peak values of gill Na+, K+-ATPase activity were detected on day 3 for all Na+/K+ ratios of saline groundwaters, then the enzyme activities descended, and on day 9 the enzyme activities achieved steady state,
and the gill HCO3
−-ATPase activity increased rapidly and achieved steady state after one day. During steady state, the gill Na+, K+-ATPase and HCO3
−-ATPase activity of Na+/K+ ratios 20 and 40 treatments were significantly higher than those in the control group (Na+/K+ ratio 27.5), while there were no significant differences between the Na+/K+ ratio 30 treatment and the control group; the gill Na+, K+-ATPase activity of Na+/K+ ratio 20 and 40 treatments were significantly higher than that for ratio 30 treatment, but there were no significant differences
of gill HCO3
−-ATPase activity among these treatments. At the end of the 15-day experiment, the weight gain (%) and specific growth rate
(SGR) of flounders maintained in seawater were significantly higher than those in groundwaters; significant differences also
occurred among the treatments; Na+/K+ ratio 30 treatment had the highest values (33.7% and 1.94 respectively), which were significantly higher than those under
Na+/K+ ratios 20 and 40 treatments. Therefore, for the saline groundwater used in this experiment, it is suggested that the Na+/K+ ratio be adjusted to approximately 30, i.e., as close to that of natural seawater as possible in the culture of flounder. 相似文献
18.
Chemical characteristics of water environment in limnic wetlands in the western Songnen Plain, China 总被引：1，自引：1，他引：0
Duetoregionalhydrogeologicandclimaticconditions,inthewesternSongnenPlain,themostdistinctcharacteristicofwaterenvironmentinlimnicwetlandsisthatmanywaterbodieshavehighsaltcontentsandpHvalues,andsomelimnicwetlandsbelongtosaltmarshes.Thelimnicwetlandsofthisregionarespecialnaturecomplex.Itisimportantfortheutilizationofwetlandresourcesandtheintegrateddevelopmentofsaline-alkalinesoiltostudychemicalcharacteristicsofwaterenvironmentinthisregion.1NATURALENVIRONMENTCHARACTERISTICSOFTHEWE… 相似文献
19.
ＩＮＴＲＯＤＵＣＴＩＯＮＯｖｅｒｔｈｅｐａｓｔｄｅｃａｄｅ ,Ｈａｅｍａｔｏｃｏｃｃｕｓｐｌｕｖｉａｌｉｓｈａｓｂｅｅｎａｓｕｂｊｅｃｔｏｆｎｕｍｅｒｏｕｓｉｎｖｅｓｔｉｇａｔｉｏｎｓｂｅ ｃａｕｓｅｉｔｓｃｏｎｓｐｉｃｕｏｕｓｒｅｄｃｏｌｏｒｐｏｉｎｔｓｔｏｉｔｓｐｏｓｓｉｂｉｌｉｔｙａｓａｈｏｐｅｆｕｌｒｅｇｅｎｅｒａｔｉｖｅｂｉｏｒｅｓｏｕｒｃｅｏｆａｓｔａｘａｎ ｔｈｉｎ .Ｅｘｔｅｎｓｉｖｅｓｔｕｄｙｏｆｃｕｌｔｕｒｅｆａｃｔｏｒｓ (ｓｕｃｈａｓｔｅｍｐｅｒａｔｕｒｅ,ｉｌｌｕｍｉｎａｔｉｏ… 相似文献
20.
Both nitrate (NO3−) and soluble reactive phosphate (PO43−) concentration in the freshwater end-member at the mouth of the Changjiang River have increased dramatically since the 1960s.
Within the same period in the sea area, with surface salinity>30, NO3− concentration has shown an obvious increase, PO43− has not changed greatly and dissolved reactive silica (SiO32−) has deceased dramatically. An examination of the elemental ratio of NO3− to PO43− at the mouth of the Changjiang River did not show a systematic trend from the 1960s to 2000s largely because both nutrients
increased simultaneously. In comparison, the elemental ratio of dissolved inorganic nitrogen (DIN) to PO43− in surface seawater, with salinity>22, has shown a clearly increasing trend. Furthermore, an overall historical change of
the SiO32−:PO43− ratio has undergone a reverse trend in this area. Based on the changes of SiO32−:PO43− and DIN:PO43− ratios, we can conclude that an overall historical change of SiO32−:DIN ratio has decreased in this area from the 1950–1960s to 2000s. The argument that phytoplankton productivity in the Changjiang
estuary has been enhanced by increasing nutrient input from the riverine transport was supported by these results. A comparative
study analyzing the shift of phytoplankton composition from the mid-1980s to 2000s was also made. The results indicated that
the average yearly percentage of diatom species in the Changjiang estuary has decreased from 84.6% during 1985–1986 to 69.8%
during 2004–2005. Furthermore, the average yearly percentage of diatom abundance in the Changjiang estuary decreased from
99.5% during to 75.5% over the same time period, while the abundance of dinoflagellates has increased dramatically, from 0.7%
to 25.4%. 相似文献