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Phylogenomic analysis of transcriptomic sequences of mitochondria and chloroplasts of essential brown algae (Phaeophyceae) in China 总被引：1，自引：1，他引：0
JIA Shangang WANG Xumin LI Tianyong QIAN Hao SUN Jing WANG Liang YU Jun REN Lufeng YIN Jinlong LIU Tao WU Shuangxiu 《海洋学报(英文版)》2014,33(2):94-101
The chloroplast and mitochondrion of brown algae （Class Phaeophyceae of Phylum Ochrophyta） may have originated from different endosymbiosis. In this study, we carried out phylogenomic analysis to distinguish their evolutionary lineages by using algal RNA-seq datasets of the 1 000 Plants （1KP） Project and publicly available complete genomes of mitochondria and chloroplasts of Kingdom Chromista. We have found that there is a split between Class Phaeophyceae of Phylum Ochrophyta and the others （Phylum Cryptophyta and Haptophyta） in Kingdom Chromista, and identified more diversity in chloroplast genes than mitochondrial ones in their phylogenetic trees. Taxonomy resolution for Class Phaeophyceae showed that it was divided into Laminariales-Ectocarpales clade and Fucales clade, and phylogenetic positions of Kjellmaniella crassi-folia, Hizikia fusifrome and Ishige okamurai were confirmed. Our analysis provided the basic phylogenetic relationships of Chromista algae, and demonstrated their potential ability to study endosymbiotic events. 相似文献
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Minicistron from a gene of prawn white spot bacilliform virus (WSBV) and its expression 总被引：1，自引：1，他引：0
By the sequencing and analysis of genomic DNA and cDNA of WSBV, it was found that these existed a minicistnni in the leading sequence of p204 gene.The minicistron utilized rare oodons of genes from prawn and WSBV.The p204 gene could be expressed in Escherichia coli whether a miniciston exited in its leading sequence or not, but the amounts of the expressed products of them were different.The minicistron might be related to the expression of p204 gene. 相似文献
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CHU Wuying YU Lian QIAN Ronghu MENG Tao ZHOU Ruixue FU Guihong CHEN Ji ZHANG Jianshe 《海洋学报(英文版)》2008,27(5):126-133
One adult α-globin gene and one β-globin gene have been cloned from the large yellow croaker Pseudosciaena crocea. Linkage analysis indicated that the α- and β-globin genes were oriented head-to-head relative to each other. To identify the regulatory elements present in the intergenic and intragenic regions of the globin complex, the intergenic region alone or together with the β-globin gene first intron was cloned into the luciferase-reporter vector pGL3-Basic respectively, and the chimeric constructs were tran- siently transfected into Vero cells and primary fish erythrocytes. The intergenic region cannot support the high-level expression of luciferase. However, the promoter activity of the intergenic region was strongly stimulated by the positive regulatory elements （PRE） located in the β-globin gene intron 1. Thus, it is proposed that the intergenic promoters and intragenic PRE were necessary for the effective expression of the linked α- and β-globin genes. 相似文献
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In order to clarify the phylogeny and relationships of the most confused hypotrichous ciliates, Holosticha-complex, four closely related holostichids (five populations), Holosticha bradburyae, H. diademata, Anteholosticha sp., and A. manca, were compared and analyzed using ITS2 secondary structures, ITS1-5.8S-ITS2 region and SSrRNA gene sequences. The ITS1-5.8S-ITS2 region sequences of these four species were first sequenced, and they shared sequence identities ranging from 68.0% to 90.1%, while two populations of Anteholosticha sp. differed in three nucleotides (sequence identity 99.8%). There were several minor differences among ITS2 secondary structures of these species, while two populations of Anteholosticha sp. had the identical secondary structure. Phylogenetic trees inferred from the ITS1-5.8S-ITS2 region sequences of stichotrichs using multiple algorithms (Neighbor-Joining, Maximum Parsimony and Bayesian) revealed similar topologies. The results show that:(1) Holosticha bradburyae and H. diademata firmly clustered together with strong bootstrap supports, forming a sister clade with Anteholosticha sp., (2) Anteholosticha appeared to be a paraphyletic assemblage, in which the morphotype A. manca was more closely related to Diaxonella trimarginata than to its congener Anteholosticha sp. Phylogenetic analyses based on the SSrRNA gene and the combined sequences of SSrRNA gene and ITS1-5.8S-ITS2 region revealed the similar relationships between Holosticha and Anteholosticha, nevertheless their positions within the subclass Stichotrichia differed from each other inferred from different genes. 相似文献
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创伤弧菌是一种可感染人类的河口病原菌。建立快速，特异而敏感的检测方法，有助于创伤弧菌感染的早期疾病诊断和及时治疗。本研究设计了针对vvhA基因的一系列引物（包括两对外部引物和两对内部引物），采用环介导等温扩增技术（LAMP）来检测创伤弧菌。结果显示，本方法的最适扩增温度是63℃，反应仅需35分钟。扩增产物不仅可以用含有DNA ladder的琼脂糖凝胶电泳检出，也可借助钙黄绿素直接肉眼观察。采用45株菌株检测该方法的特异性，其中所有创伤弧菌均被检出而其他菌株检测结果皆为阴性。本方法的敏感性是普通PCR扩增的100倍，同时利用该方法可以准确检测出所有的模拟样品、临床标本及环境样品。与其他已知方法比较，针对vvhA基因的介导等温扩增技术可以快速、简单、敏感及特异地鉴定创伤弧菌。 相似文献
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N. P. Romanov 《Izvestiya Atmospheric and Oceanic Physics》2009,45(6):799-804
Instead of approximation formula ln(E(t)/E(0)) = [(a ? bt)t/(c + T)] commonly used at present for representing dependence of pressure of saturated streams of liquid water E upon temperature we suggested new approximation formula of greater accuracy in the form ln(E(t)/E(0)) = [(A ? Bt + Ct 2)t/T], where t and T are temperature in °C and K respectively. For this formula with parameters A = 19.846, B = 8.97 × 10?3, C = 1.248 × 10?5 and E(0) = 6.1121 GPa with ITS-90 temperature scale and for temperature range from 0°C to 110°C relative difference of approximation applying six parameter formula by W. Wagner and A. Pruß 2002, developed for positive temperatures, is less than 0.005%, that is approximately 15 times less than accuracy obtained with the firs formula. Increase of temperature range results in relative difference increasing, but for even temperature range from 0°C to 220°C it does not higher than 0.1%. For negative temperatures relative difference between our formula and a formula of D. M. Murphy and T. Koop, 2005, is less than 0.1% for temperatures higher than ?25°C. This paper also presents values of coefficients for approximation of Goff and Grach formula recommended by IMO. The procedure of finding dew point T d for known water steam pressure e n based on our formula adds up to solving an algebraic equation of a third degree, which coefficients are presented in this paper. For simplifying this procedure this paper also includes approximation ratio applying a coefficient A noted above, in the form T d (e n ) = \(\frac{{AT_0 }}{{A - \varepsilon }}\) + 0.0866?2 + 0.0116?10/3, where ? = ln(e n /E(T 0)). Error of dew point recovery in this ratio is less than 0.005 K within the range from 0 to 50°C. 相似文献
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Production parameters of surface phytoplankton were measured along three transects: La Manche-Cape Town (I); Cape Town-54°S (II); 0°-49°W (along 54°S) (III). The Canary upwelling waters were most productive along transect I, where the surface chlorophyll a (Chl 0) and the surface primary production (PP 0) were as high as 4.3 mg/m3 and 173 mg C/m3 per day, respectively. Mosaic patterns in the distribution of these parameters were recorded in the northeastern regions of the South Subtropical Anticyclonic Gyre (Chl 0 = 0.03–0.35 mg/m3; PP 0 = 1.6–12.6 mg C/m3 per day). Along transect II, the average twofold southward increase in Chl 0 (from 0.2 to 0.4 mg/m3) and the concurrent decline of the phytoplankton assimilation activity ( AN 0) resulted in deviations from typical latitudinal changes inPP 0. At most sites, PP 0 values varied between 6 and 15 mg C/m3 per day. Negligible changes in Chl 0 (0.36–0.85 mg/m3), PP 0 (8–19 mg C/m3 per day), and AN 0 (0.7–1.6 mg C/mg chl a per hour) were registered for the oceanic waters along transect III. Along all the transects, PP 0 depended on Chl 0 to a greater extent than AN 0. The values of the latter parameter were largely determined by the water temperature and showed a slight correlation with the insolation. Along transect II, the integrated primary production (PP int) and the layer-integrated chlorophyll a in the upper 200 m (Chl 0–200) generally varied from 180 to 360 mg C/m2 per day and from 30 to 70 mg/m2, respectively. In the Polar Front region, an increase in Chl 0–200, PP int, Chl 0, and PP 0 up to respective values of 190 mg/m2, 520 mg C/m2 per day, 1.2 mg/m3, and 32 mg C/m3 per day was observed. A comparison of the water column (0–100 m) stability with the vertical distribution of the primary production and chlorophyll content along transect II implies that the thick (>100 m) upper mixed layer (UML) formed in response to the strong water cooling and wind forcing was largely responsible for the limited primary production in the Subantarctic and Antarctic regions. The large UML thickness resulted in an intense removal of plant cells from the photosynthetic layer and light starvation of a significant (up to 60%) part of the phytoplankton community. 相似文献
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As a major aldehyde pollutant widely existing in industry and our daily life, acetaldehyde is more and more harmful to human health. As characteristic habitat niche, bacteria from deep sea environments are abundant and distinctive in heredity, physiology and ecological functions. Thus, the development of acetaldehyde-degrading bacteria from deep sea provides a new method to harness acetaldehyde pollutant. Firstly, in this study,acetaldehyde-degrading bacteria in the deep sea water of the West Pacific Ocean were enriched in situ and in the laboratory respectively, and then the diversity of uncultured bacteria was studied by using 16 S r RNA genes. Then acetaldehyde-degrading strains were isolated from two samples, including enrichment in situ and enrichment in laboratory samples of deep sea water from the West Pacific Ocean using acetaldehyde as the sole carbon source,and then the ability of acetaldehyde degradation was detected. Our results showed that the main uncultured bacteria of two samples with different enrichment approaches were similar, including Proteobacteria,Actinobacteria, Firmicutes, Cyanobacteria, but the structure of bacterial community were significant different.Four subgroups, α, γ, δ and ε, were found in Proteobacteria group. The γ-Proteobacteria was dominant(63.5%clones in laboratory enriched sample, 75% clones in situ enriched sample). The species belonged to γ-Proteobacteria and their proportion was nearly identical between the two enrichment samples, and Vibrio was the predominant genus(45% in laboratory enriched sample, 48.5% in situ enriched sample), followed by Halomonas(9% in situ enriched sample) and Streptococcus(6% in laboratory enriched sample). A total of 12 acetaldehyde-degrading strains were isolated from the two samples, which belonged to Vibrio, Halomonas,Pseudoalteromonas, Pseudomonas and Bacillus of γ-Proteobacteria. Strains ACH-L-5, ACH-L-8 and ACH-S-12,belonging to Vibrio and Halomonas, have strong ability of acetaldehyde degradation, which could tolerate 1.5 g/L acetaldehyde and degrade 350 mg/L acetaldehyde within 24 hours. Our results indicated that bacteria of γ-Proteobacteria may play an important role in carbon cycle of deep sea environments, especial the bacteria belonging to Vibrio and Halomonas and these strains was suggested for their potentials in government of aldehyde pollutants. 相似文献
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Results obtained from simulating the propagation of infrasonic waves from the Chelyabinsk meteoroid explosion observed on February 15, 2013, are given. The pseudodifferential parabolic equation (PDPE) method has been used for calculations. Data on infrasonic waves recorded at the IS31 station (Aktyubinsk, Kazakhstan), located 542.7 km from the likely location of the explosion, have been analyzed. Six infrasonic arrivals (isolated clearly defined pulse signals) were recorded. It is shown that the first “fast” arrival (F) corresponds to the propagation of infrasound in a surface acoustic waveguide. The rest of the arrivals (T1–T5) are thermospheric. The agreement between the results of calculations based on the PDPE method and experimental data is satisfactory. The energy E of the explosion has been estimated using two methods. One of these methods is based on the law of conservation of the acoustic pulse I, which is a product of the wave profile area S/2 of the signal under analysis and the distance to its source E I [kt] = 1.38 × 10–10 (I [kg/s])1.482. The other method is based on the relation between the energy of explosion and the dominant period T of recorded signal E T [kt] = 1.02 × (T [s]2/σ)3/2, where σ is the dimensionless distance determining the degree of nonlinear effects during the propagation of sound along ray trajectories. According to the data, the explosion energy E I,T ranges from 1.87 to 32 kt TNT. 相似文献
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The deviations of the marine surface slope spectra (measured using an array of wave gauge sensors) from the theoretical estimates obtained using the linear spectral model of the wave field are analyzed. It has been indicated that the average measured full slope spectra (the sum of the slope component spectra in the orthogonal directions) is higher than the theoretical estimates by 6% at frequencies from the surface wave spectral peak (f m ) to 4.5 f m . The difference between the measured and theoretical estimates of the full slope spectrum rapidly increases at frequencies of f < f m . At f m ≈ 0.75 f m , the average measured full slope spectrum is higher than the theoretical estimate by a factor of more than 5. 相似文献
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Despite its potential significance for industrial utilization, any activities associated with the mining of manganese (Mn) nodules might have substantial impacts on benthic ecosystems. Because microorganisms respond quickly to changing environmental conditions, a study of microbial communities provides a relevant proxy to assess possible changes in benthic ecosystems associated with mining activities. We investigated fine-scale microbial community composition and diversity inside and on the surface of Mn nodules and in nearby deep-sea sediments in the Korea Deep Ocean Study (KODOS) area located in the Clarion-Clipperton Fracture Zone (CCFZ) of the northeast equatorial Pacific. Although microbial cell density was lower within nodules (3.21 × 106 cells g-1) than in sediment (2.14 × 108 cells g-1), nodules provided a unique habitat for microorganisms. Manganese-oxidizing bacteria including Hyphomicrobium and Aurantimonas in Alphaproteobacteria and Marinobacter in Gammaproteobacteria were abundant in nodules, which implied that these bacteria play a significant role in nodule formation. In contrast, Idiomarina in Gammaproteobacteria and Erythrobacter and Sulfitobacter in Alphaproteobacteria were abundant in sediments. Meanwhile, Thaumarchaeota, a phylum that consists of ammonia-oxidizing chemolithoautotrophs, were the predominant archaeal group both in nodules and sediment. Overall, microbial communities in Mn nodules were unique compared to those observed in sediments. Furthermore, the phylogenetic composition of microorganisms in the KODOS area was distinguishable from that in the nodule provinces claimed by China and Germany in the CCFZ and nodule fields in the central South Pacific Gyre, respectively. 相似文献
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腹壁纤毛虫是一种附着在浮游动物上的原生生物，尤其是附着在桡足类和甲壳类上的。而缘毛虫是可以以自由或寄生的形式在世界各沿海地区和港湾处被发现。本研究首次对东印度洋表层至200 m深海域的缘毛类纤毛虫以及其水平分布展开了研究。根据纤毛虫的形态、纤毛结构和茎的形态等的特征，在2014年4月10日至5月13日东印度洋航次的浮游生物拖网样品中鉴定出了五种富腹纤毛虫，海洋钟形虫Vorticella oceanica、交替聚缩虫Zoothamnium alternans、阿拉聚缩虫Zoothamnium alrasheidi、远洋聚缩虫Zoothamnium pelagicum和海洋聚缩虫Zoothamnium marinum。并通过光显微镜和扫描显微镜拍摄的照片，说明了其形态、尺寸和特征。研究结果表明，海洋钟形虫和远洋聚缩虫的分布与硅藻（如，密聚角毛藻Chaetoceros coarctatus）和桡足类（如，短角长腹剑水蚤Oithona brevicornis）等寄主有关，也包括甲藻（如，三角角藻Ceraitum tripose）周围的一些个体。这些附着纤毛虫的分布在东印度洋海域东南区以海洋钟形虫、远洋聚缩虫和海洋聚缩虫为主，而在孟加拉湾北部则有大量的阿拉聚缩虫。这种分布的变化可能是与寄主的分布有关，如桡足类（短角长腹剑水蚤）和硅藻（密聚角毛藻）的分布影响。 相似文献