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1.
致病性副溶血弧菌脂多糖的制备及其对牙鲆的免疫效应 总被引：4，自引：1，他引：4
利用酚水法提取的副溶血弧菌脂多糖 (lipopolysaccharide,L PS) ,经 SDS- PAGE电泳及银染色后表明这种方法提取副溶血弧菌脂多糖显示出 4 1 .3KDa,30 .7KDa,2 5 .4 KDa以及 1 1 .8KDa 4条主要脂多糖带。用此做免疫原 ,采用加强腹腔注射法免疫牙鲆 ,测定牙鲆的血清凝集抗体效价和白细胞吞噬活性。结果表明 ,免疫后的牙鲆对副溶血弧菌的血清凝集抗体效价有显著提高 ,第 3周抗体滴度达到最高值 2 5 6 ;白细胞的吞噬活性与对照组相比有明显加强 (P<0 .0 5 )。 相似文献
2.
凡纳滨对虾高密度养殖实验 总被引：3，自引：0，他引：3
实验时间 4 0天 ,放苗密度 5 0 0尾 /m2 、10 0 0尾 /m2 。凡纳滨对虾初体长为 2 .5 88±0 .341cm ,初体重 (0 .2 39± 0 .0 86 ) g。养殖水体中放置筛绢隔断 ,在不换水的条件下 ,监测水体中无机磷、氨氮的变化趋势 ,初步实验高密度和分隔养殖的效果。结果表明 ,高、低密度氨氮、无机磷差异均为极显著 (P <0 .0 1) ;成活率、产量差异均为显著 (P <0 .0 5 )。高、低密度特定生长率 体重 差异不显著 (P >0 .0 5 )。观察到不同时间不同密度凡纳滨对虾对筛绢隔断的反应不同 ,表明对虾能利用隔断筛绢 ,从而有效提高养殖容量 相似文献
3.
南黄海鳀鱼产卵场小型底栖生物的丰度和生物量 总被引：4，自引：0，他引：4
20 0 0年 6月在南黄海鱼产卵场 18个站位的调查结果表明 ,小型底栖生物的平均丰度为 0 .81× 10 6ind· m-2 (± 0 .4 1× 10 6ind· m-2 ) ,平均生物量为 1.2 2 g dwt· m-2 ,小型底栖生物的丰度和生物量的水平分布图型大体相似 ,即高的数量值分布在等深线 5 0 m以浅的与海岸线平行的74 94、7994、85 94、11394等站。小型底栖生物数量与 10种环境因子的 Pearson相关分析表明 ,丰度与水深呈负相关 (r,- 0 .5 5 5 ,P<0 .0 5 ) ,与 Chl- a呈正相关 (r,0 .4 86 ,P<0 .0 5 ) ;生物量与水深、沉积物中的粘土含量 (% )和水含量 (% )均呈负相关 (r,0 .4 84～ 0 .5 0 0 ,P<0 .0 5 )。共鉴定出 18个小型生物类群 ,线虫是数量占优势的类群 ,平均丰度 0 .6 0× 10 6ind· m-2 (± 0 .36× 10 6ind· m-2 ) ,占总丰度的 73.8% ,其他的重要类群依次为底栖桡足类 (18.7% )、多毛类 (3.1% )、动吻类 (1.6 % )和介形类 (1.1% )。按生物量 ,优势类群依次为多毛类 (占 2 9% )、桡足类 (2 3% )和线虫 (2 0 % ) 相似文献
4.
《海洋地质与第四纪地质》2004,24(1):112-112
(引自《中国科技期刊引证报告》( 2 0 0 3年版 ) )名次 期刊名称总被引频次影响因子即年指标基金论文比来源文献量1地理学报 12 0 42 .3 0 10 .2 62 0 .98842地质学报 93 5 2 .13 3 0 .3 97 0 .84633地质论评 85 0 0 .82 10 .2 5 0 0 .75 844岩石学报 7111.197 0 .2 810 .91645冰川冻土 6742 .42 60 .412 0 .87 13 66沉积学报 62 2 0 .75 10 .0 860 .681167地理科学 5 87 1.0 2 0 0 .10 40 .8413 58地理研究 5 5 91.4180 .2 780 .98909地质科学 5 180 .6890 .15 5 0 .745 810矿床地质 3 92 0 .7690 .3 2 7 0 .895 211山地学报 3 77 0 .5 2 … 相似文献
5.
甲醛对Nessler法和次氯酸酚盐法测定养殖水体总氨氮的影响 总被引：1，自引：0，他引：1
水体中含一定浓度的甲醛 ,用 Nessler法测得的总氨氮 (TAN)浓度远远高于实际浓度 ,差异百分数最高达 4 5 0 .0 6 % ;用次氯酸酚盐法测得的总氨氮 (TAN)浓度则明显低于实际浓度 ,差异百分数最高可达 84 .77%。甲醛浓度范围为 10～ 4 0 mg/ L、TAN浓度为 1～ 10 mg/ L,通过双因素有重复观察值的交叉实验 ,得到如下矫正式 :(1)用 Nessler法测 TAN时 ,Y=4 .0 5 2 9- 0 .0 72 4 x1+ 0 .2 0 6 4x2 ,标准误差为 1.3381;(2 )用次氯酸酚盐法测 TAN时 ,Y=0 .1196 + 0 .0 345 x1+ 1.0 0 2 2 x2 ,标准误差为 0 .4 6 2 7。 Y为 TAN实际浓度 (mg/ L) ,x1为甲醛浓度 (mg/ L) ,x2 为测得的 TAN浓度(mg/ L )。比较含氨氮水体与不含氨氮水体中的甲醛浓度 ,方差分析结果表明 ,没有显著差异。 相似文献
6.
摄食水平对褐牙鲆幼鱼能量收支的影响 总被引：3，自引：0，他引：3
褐牙鲆幼鱼 (体重 0 .71～ 4 .16 g;体长 4 .4 5～ 8.14cm)在 2 4℃下保持 5个摄食水平 (0～最大 )。投喂人工配合饵料。褐牙鲆能量和干物质的吸收效率随摄食水平的增加而增加 ,平均为97.38%和 76 .73%。褐牙鲆排粪能 (F )占摄食能的比例为 0 .95 %～ 2 .83% ,随摄食水平的增加而减少 ;氮排泄 (U)占摄食能比例为 5 .5 2 %～ 6 .77% ,各摄食水平下变化不大 ;标准代谢耗能 (Rs)占摄食能比例为 1.0 5 %～ 7.82 % ,随摄食水平的增加而减少 ;在各摄食水平条件下 ,摄食代谢耗能(Rf)占摄食能比例变化不大 ,平均为 4 5 .72 % ;生长能 (G)占摄食能比例为 32 .6 7%～ 4 9.2 1%。随摄食水平增加而增加。在最大摄食水平时 ,褐牙鲆能量分配形式为 :10 0 C =0 .95 F + 5 .5 2 U +4 4.31R+ 4 9.2 1G 相似文献
7.
《海洋地质与第四纪地质》在年度中国科技期刊引证报告 《海洋地质与第四纪地质》2001,21(1):86
《海洋地质与第四纪地质》在 1999年度中国科技期刊引证报告 (2 0 0 0年版 )中国科技期刊 (1372种 )地质与地理学科类中 ,影响因子排序第 2 6名 (表 1) ;被引频次排序第 19名 (表 2 )。表 1 1999年中国科技期刊地质与地理学科类影响因子排序名次期刊名称 影响因子 即年指标1矿床地质 1.4870 .1112第四纪研究 1.195 0 .1963地质学报 0 .8480 .3 5 14地质论评 0 .80 70 .1835地理学报 0 .7810 .13 36地学前缘 0 .65 3 0 .1987岩石学报 0 .62 5 0 .2 0 38岩矿测试 0 .5 670 .0 5 49地质科学 0 .5 13 0 .17910冰川冻土 0 .45 5 0 .10 311岩石… 相似文献
8.
海洋弧菌碱性蛋白酶的分离纯化及部分性质研究 总被引：2，自引：0，他引：2
采用硫酸铵沉淀、Sephadex- 75 ,Sephadex- 10 0凝胶过滤层析等方法纯化海洋弧菌 (Vibriop acini) X4 B- 7菌株产生的碱性蛋白酶 ,得到电泳纯酶制品 ,并对纯化酶的性质进行了研究。结果显示 :纯酶的分子量为 2 7KD,等电点 p I=8.7,最适反应 p H9.0～ 10 .5 ,最适反应温度 5 0～ 6 0℃。ED-TA对酶活力没有影响 ,高酶浓度可以降低 SDS对酶的抑制作用 ,该酶可用于解聚组蛋白。 DNA琼脂糖凝胶电泳证明 :酶对 DNA酶有降解作用 ,而对 DNA没有降解作用 ,该酶有希望应用于核酸的提取 相似文献
9.
Wave Energy Study in China: Advancements and Perspectives 总被引：3，自引：0，他引：3
1 .Ｉｎｔｒｏｄｕｃｔｉｏｎ1ＴｈｉｓｓｕｂｊｅｃｔｗａｓｓｕｐｐｏｒｔｅｄｂｙｔｈｅＨｉｇｈＴｅｃｈＲｅｓｅａｒｃｈａｎｄＤｅｖｅｌｏｐｍｅｎｔ ( 863)Ｐｒｏｇｒａｍ (ＧｒａｎｔＮｏ .2 0 0 1ＡＡ5 160 10 ) ,ｔｈｅＮａｔｉｏｎａｌＮａｔｕｒａｌＳｃｉｅｎｃｅＦｏｕｎｄａｔｉｏｎｏｆＣｈｉｎａ (ＧｒａｎｔＮｏ .5 99760 4 7ａｎｄＮｏ .5 99790 2 5 ) ,ｔｈｅＳｃｉｅｎｃｅａｎｄＴｅｃｈｎｉｃＰｒｏｇｒａｍｏｆＧｕａｎｇｄｏｎｇＰｒｏｖｉｎｃｅ (ＧｒａｎｔＮｏ .Ｃ32 0 0 4 ) ,ａｎｄＮａｔｉｏｎａｌＳｃｉｅ… 相似文献
10.
渤海小型底栖生物的丰度和生物量 总被引：9，自引：1，他引：9
该文是渤海 1997年 6月、1998年 9月和 1999年 4月 3个航次小型底栖生物调查结果。结果表明 ,3个航次小型底栖生物的平均丰度分别为 :(2 30 0± 12 0 6 ) ind/ (10 cm2 )、(86 9± 5 10 ) ind/(10 cm2 )和 (6 32± 4 0 0 ) ind/ (10 cm2 )。平均生物量分别为 :(15 2 1± 6 34) μg(dwt) / (10 cm2 )、(72 5±35 4 )μg (dwt) / (10 cm2 )和 (5 17± 393)μg (dwt) / (10 cm2 )。共鉴定出 14个小型底栖生物类群 ,其中自由生活海洋线虫丰度占绝对优势 ,桡足类丰度居第 2位 ,这两个类群总和占小型底栖生物总丰度的 94 .8%～ 97.5 %。在生物量中所占比例列前 4位的类群依次为线虫、多毛类、桡足类、双壳类 ,加起来超过小型底栖生物总生物量的 80 %。小型底栖生物的 74 %分布于 2 cm以浅表层中。小型底栖生物的丰度和生物量在渤海海峡和渤海中东部较高 ,与环境因子的相关分析表明小型底栖生物的丰度与水深呈极显著的正相关 ,与沉积物的中值粒径呈显著的负相关 相似文献
11.
Chandralata Raghukumar G. Sheelu P. A. Loka Bharathi Shanta Nair C. Mohandass 《Marine Georesources & Geotechnology》2001,19(1):1-16
In order to assess the impact of deep-sea mining on the in situ benthic life, we measured the microbial standing stock and concentration of organic nutrients in the deep-sea sediments of the Central Indian Ocean Basin in the Indian pioneer area. Sediments were collected using box core and grab samples during September 1996. The total bacterial numbers ranged from 10 10 -10 11 cells per g -1 dry weight sediment. There was a marginal decrease in the number of bacteria from surface to 30 cm depth, though the subsurface section registered a higher number than did the surface. The highest numbers were encountered at depths of 4-8 cm. The retrievable number of bacteria were two orders less in comparison with the direct total counts of bacteria. An almost homogeneous distribution of bacteria, total organic carbon, living biomass, and lipids throughout the depth of cores indicates active microbial and benthic processes in the deep sea sediments. On the other hand, a uniform distribution of total counts of bacteria, carbohydrates, and total organic carbon in all the cores indicates their stable nature and suggests that they can serve as useful parameters for long-term monitoring of the area after the benthic disturbance. Further studies on temporal variability in this region would not only verify the observed norms of distribution of these variables but would also help to understand restabilization processes after the simulated benthic disturbance. 相似文献
12.
Arne Schnack-Friedrichsen Angela M. Davis Jim D. Bennell Dei G. Huws 《Marine Georesources & Geotechnology》2001,19(4):221-243
The interdependence between the seismo-acoustic properties of a marine sediment and its geotechnical/physical parameters has been known for many years, and it has been postulated that this should allow the extraction of geotechnical information from seismic data. Though in the literature many correlations have been published for the surficial layer, there is a lack of information for greater sediment depths. In this article, a desktop study on a synthetic seafloor model illustrates how the application of published near-surface prediction equations to subsurface sediments (up to several tens of meters burial depth) can lead to spurious predictions. To test this further, acoustic and geotechnical properties were measured on a number of sediment core samples, some of which were subjected to loading in acoustically-equipped consolidation cells (oedometers) to simulate greater burial depth conditions. For low effective pressures (representing small burial depths extending to around 10 meters subsurface), the general applicability of established relationships was confirmed: the prediction of porosity, bulk density, and mean grain size from acoustic velocity and impedance appears generally possible for the investigated sedimentary environments. As effective pressure increases through, the observed relationships deviate more and more from the established ones for the near-surface area. For the samples tested in this study, in some instances increasing pressure even resulted in decreasing velocities. There are several possible explanations for this abnormal behavior, including the presence of gas, overconsolidation, or bimodal grain size distribution. The results indicate that an appropriate depth correction must be introduced into the published prediction equations in order to obtain reliable estimates of physical sediment properties for greater subsurface depths. 相似文献
13.
Geoacoustic Inversion via Genetic Algorithm and Its Application to Manganese Sediment Identification
An acoustic inversion method using a wide-band signal and two near field receivers is proposed and applied to multiple layered seabed models including a manganese sediment. The inversion problem can be formulated into a probabilistic model comprised of signals, a forward model, and additive noise. The forward model simulates wide-band signals, such as chirp signals, and is chosen to be the source-waveletconvolution plane wave modeling method. The wavelet matching technique, using weighted least-squares fitting, estimates the sediment sound-speed and thickness on which determination of the possible numerical ranges for a priori uniform distribution is based. The genetic algorithm is applied to a global optimization problem to find a maximum a posteriori solution for determined a priori search space. Here the object function is defined by an L 2 norm of the difference between measured and modeled signals. Not only the marginal pdf but also its statistics are calculated by numerical evaluation of integrals using the samples selected during importance sampling process of the genetic algorithm. 相似文献
14.
海上大直径钢管桩打桩过程中,桩周土体受到强烈扰动而发生强度弱化,掌握桩周土体强度弱化规律对于准确预测打桩过程、保证工程安全具有重要意义。为研究土体强度弱化规律,开展了环剪试验模拟打桩对桩周土体的扰动,测试土体强度随剪切速率的变化规律,建立了描述土体强度弱化规律的拟合公式,引入到打桩分析软件中。研究结果表明：土体的强度折减程度不仅与土体本身的性质有关还受到土体的埋深和剪切速率的影响,埋深越深土体强度折减程度越低,剪切速率越高土体强度折减越高,在打桩分析中可采用这里推荐的线性折减方法来模拟不同深度处土体强度的折减规律。 相似文献
15.
Shigehisa Nakamura 《Marine Geodesy》2000,23(1):55-61
Specific properties of the interannual sea level variations and annual tides in the Northwestern Pacific were studied. Several tide stations were monitored. The monthly mean sea level for the year of 1995 was analyzed at each tide station. A seismic event in 1995, some tectonic activity around the subject area, and the Kuroshio (the oceanic western boundary current) may possibly contaminate results which would have occurred from the astronomical annual tide alone. 相似文献
16.
The effect of different fixation and storage protocols on the flow cytometric (FCM) simultaneous analysis of bacterioplankton and phytoplankton in coastal seawater samples (Mediterranean coastal lagoons) was investigated. FCM measurements (cell number, fluorescence and scatter characteristics) were obtained through DAPI staining. Three fixatives [glutaraldehyde (GA), formaldehyde (FA) and paraformaldehyde (PFA)] and two storage (3 months duration) methods (5 °C and −196 °C) were tested. Two dominant populations were detected in studied samples: bacteria and eukaryotic picophytoplankton. Adding fixatives (2% final concentration) appears necessary to obtain FCM exhaustive counts of all the bacteria and phytoplanktonic cells. This was related to the permeation effect of fixatives which allowed a better DAPI staining of the cells. Maximum fluorescence, i.e. optimal staining of the cells was obtained with FA or PFA, and significant lower fluorescences with GA. Fixed samples stored at 5 °C induced rapid cell loss. Only storage in liquid nitrogen of samples fixed with FA or PFA, allows mid-term (≥4 months) preservation of bacteria or picophytoplankton cell numbers, and limited evolution of DAPI-induced fluorescence and scatter characteristics. 相似文献
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18.
Oedometer tests have been carried out on 70 undisturbed surficial clays (at approximately 250 mm below the mudline), mostly collected by free-fall corers from sites widely scattered throughout the deep-sea North Atlantic. Acoustic measurements were also made, initially on contiguous samples and ultimately on the same sample using a geophysically instrumented oedometer which also collected electrical resistivity data. Apart from those quiescent areas below the carbonate compensation depth, such as north of the West Indies where very fine clays exist, most of the samples are silty clays whose geotechnical-geophysical properties are dependent on the type of clay minerals present (and their ability to take in moisture), the sand-size fraction, and the quantity of carbonate present. Thus the pure clays have high compressibilities which decrease on the addition of coarse particles, while the converse is true for the acoustic parameters, these increasing with the sand fraction. Using the notion of the intrinsic compression line for all samples, and comparison to it of the measured compression curves, it is clear that, contrary to some previously held ideas, most deep-sea clays are normally consolidated; the addition of carbonate has the effect of creating an open, stronger sediment skeleton. Interestingly, where information is available, the variation with depth of a sample's acoustic velocity follows the void ratio pressure relationship of the compression curve. This allows the construction of an in-situ sediment compression curve using the in-situ geophysical observations. 相似文献
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《海洋学报(英文版)》2014,(9):F0003-F0003
<正>Acta Oceanologica Sinica(AOS)is a comprehensive academic journal edited by the Editorial Committee of Acta Oceanologica Sinica and is designed to provide a forum for important research papers of the marine scientific community which reflect the information on a worldwide basis.The journal publishes scholarly papers on marine science and technology,including physics,chemistry,biology, 相似文献