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凡纳滨对虾高密度养殖实验 总被引：3，自引：0，他引：3
实验时间 4 0天 ,放苗密度 5 0 0尾 /m2 、10 0 0尾 /m2 。凡纳滨对虾初体长为 2 .5 88±0 .341cm ,初体重 (0 .2 39± 0 .0 86 ) g。养殖水体中放置筛绢隔断 ,在不换水的条件下 ,监测水体中无机磷、氨氮的变化趋势 ,初步实验高密度和分隔养殖的效果。结果表明 ,高、低密度氨氮、无机磷差异均为极显著 (P <0 .0 1) ;成活率、产量差异均为显著 (P <0 .0 5 )。高、低密度特定生长率 体重 差异不显著 (P >0 .0 5 )。观察到不同时间不同密度凡纳滨对虾对筛绢隔断的反应不同 ,表明对虾能利用隔断筛绢 ,从而有效提高养殖容量 相似文献
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In laboratory conditions, effects of rearing temperature and stocking density were examined on hatching of fertilized egg
and growth of auricularia larvae of Apostichopus japonicus respectively. Data series like larval length and density, metamorphic time, and survival rate of the larvae were recorded.
Statistics showed that for A. japonicus, survival rate (from fertilized egg to late auricularia) decreased significantly with the increasing rearing temperature
(P<0.05). At different temperatures SGR was statistically significant as well (P<0.05) from day 1, and maximal SGR was found on day 9 at 24°C (159.26±3.28). This study clearly indicated that at low temperature
(<24°C), metamorphic rate was remarkably higher than at higher temperature (>26°C). Hatching rate was significantly different
between 0.2–5 ind./ml groups and 20–50 ind./ml groups. Rearing larvae at the higher density had the smaller maximal-length,
whereas needed longer time to complete metamorphosis. This study suggested that 21°C and 0.4 ind./ml can be used as the most
suitable rearing temperature and stocking density for large -scale artificial breeding of A. japonicus’s larvae. 相似文献
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研究两种硒化物在文蛤富集、排出镉过程中的作用,对文蛤进行转录组测序,并进行生物信息学分析,以探讨硒化物对Cd2+代谢相关机制。硒化卡拉胶组和亚硒酸钠组共产出587 855条高质量reads。基因本体分析功能注释到15 380条unigenes, KEGG通路注释到18 866条unigenes。差异基因主要富集到肌球蛋白复合物(myosin complex)、离子通道抑制(ion channel suppression)等基因本体分析功能中。差异基因KEGG通路富集显示,在嘌呤代谢(purinemetabolism)、ECM受体相互作用(extracellularmatrix receptor interaction)、硒化合物代谢(metabolism of selenium compounds)等通路显著富集。从结果分析Cd2+可以使文蛤体内蛋白转录修饰出现异常;有机硒可以通过调控金属离子通道活性来排出细胞内的Cd2+;无机硒主要作用于文蛤细胞表面,增强其表面活性抵御Cd2+进入细胞;差异基因KEGG注释结果表明有机硒与无机硒在文蛤重金属代谢中作用机制以及途径不相同。 相似文献
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为了解东营河口浅海贝类生态国家级海洋特别保护区中大型底栖动物的群落结构及分布特征,于2019年对保护区内大型底栖动物进行了春、夏、秋、冬4个季度的详细调查,研究结果为:共采集大型底栖动物73种,优势种类为:彩虹明樱蛤(Moerellairidescens)、四角蛤蜊(Mactraveneriformis)、寡节甘吻沙蚕(Glycindegurjanovae)、三叶针尾涟虫(Diastylistricincta)、光滑河篮蛤(Potamocorbula laevis)、极地蚤钩虾(Pontocrates altamarimus)和马丁海稚虫(Spio martinensis)。大型底栖动物的年平均丰度为166 ind/m2,年平均生物量为14.30 g/m2,Shannon-Wiener多样性指数(H′)平均值为2.14。聚类分析结果为调查区域的大型底栖动物分为两组群落(近岸区和远岸区),近岸区域形成以光滑河篮蛤和四角蛤蜊为主的群落,远岸区域形成以彩虹明樱蛤-寡节甘吻沙蚕为优势种类的群落,两者表现出不同的群落特征。ABC曲线分析表明,保护区内的生物群落结构比较稳定,从生态容量上来看,贝类资源目前仍还有一定的增殖及保护空间。 相似文献
5.
The effect of water temperature on gut mass and digestive enzyme activity in sea cucumber Apostichopus japonicus, including relative gut mass (RGM), amylase, lipase, pepsin and trypsin activities were studied at temperatures of 7, 14,
21, and 28°C over a period of 40 days. Results show that RGM significantly decreased after 40 days at 21°C and markedly decreased
over the whole experiment period at 28°C; however, no significant effect of duration was observed at 7 or 14°C. At 14°C, trypsin
activity significantly decreased over 10 and 20 days, then increased; amylase and trypsin activity significantly decreased
after 40 days at 28°C. However, no significant effect of duration was found on amylase, pepsin or trypsin activities in the
other temperature treatment groups. At 28°C, lipase activity peaked in 20 days and then markedly decreased to a minimum at
the end of the experiment. On the other hand, pepsin activity at 28°C continuously increased over the whole experimental period.
Principle component analysis showed that sea cucumbers on day 40 in the 21°C group and in the previous 20 days in the 28°C
group were in the prophase of aestivation. At 28°C, sea cucumbers aestivated at 30–40 days after the start of the experiment.
It is concluded that the effect of temperature on the digestion of A. japonicus is comparatively weak within a specific range of water temperatures and aestivation behavior is accompanied by significant
changes in RGM and digestive enzyme activities. 相似文献
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在6~24℃室内可控温范围内,每3℃为一个梯度,以魁蚶壳长、体质量日增长率以及存活率作为指标,研究了温度对3种规格魁蚶(Scapharca broughtonii)幼贝(小规格壳长6.565 mm±0.225 mm、体质量53.704 mg±5.830 mg;中规格壳长10.114 mm±0.446 mm、体质量181.918 mg±24.797 mg;大规格壳长14.725 mm±0.315 mm、体质量562.416 mg±42.791 mg)生长和存活的影响。结果表明,3种规格魁蚶幼贝在6~24℃水温下均能生长,但在水温6℃及24℃下的壳长和体质量日增长率均较低;水温9℃时3种规格的幼贝的存活率均最高,水温24℃时3种规格幼贝的存活率均最低;水温6~21℃时3种规格幼贝的存活率随着规格的增大而提高,水温24℃时3种规格幼贝的存活率随着规格的增大而降低;小、中、大规格幼贝适宜生长温度分别为10.7~22.3、6.9~23.2、3.7~23.3℃,较适宜生长温度分别为14.3~19.5、15.8~21.6、11.5~21.0℃,最适生长温度分别为18、18、15℃。实验结论为,随着规格增大,魁蚶幼贝适宜生长温度范围扩大,最适生长温度降低,本实验确定了魁蚶幼贝适宜的生长温度,为更有效地开展魁蚶中间培育和底播增殖提供理论支持。 相似文献
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为了调查刺参(Apostichopus japonicus)免疫机能的周年变化,初步探索刺参免疫的规律,于2006年7月至2007年6月对刺参体腔液几种免疫指标进行了周年测定.结果显示,周年内刺参体腔液内几种酶类显著变化的转折点为9月、10月,1月,2月、4月和5月.刺参体腔液几种酶类活性变化与温度和盐度等环境因素并不存在相关关系,因此推测这些显著变化并不是单一的温度、盐度等环境因素作用的结果,综合分析,其变化原因可能与刺参的夏眠、生理及繁殖等因素的作用有关. 相似文献
8.
温度选择对刺参群体在不同温度下生长及热休克蛋白表达的影响 总被引：1，自引：0，他引：1
高温(26℃)培育刺参(Apostichopus japonicus)幼体获得选择群体,生长至1.03 g±1.30 g,与对照组幼参在18、20、22和24℃温度下培育。二者的成活率随着温度升高而降低,而选择组幼参24℃的成活率显著高于对照群体(P0.05)。不同温度下的特定生长率之间没有差异。TRIzol法提取刺参总RNA,获得刺参HSP70片断基因序列,利用实时定量PCR方法检测,选择群体HSP70mRNA的表达量在正常温度和热激条件下都较对照组高,但表达量升高的倍数相近,选择组为1.99倍,对照组为2.07倍。 相似文献
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