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1.
Platymonas (Tetraselmis) subcordiformis is a unicellular marine green alga. It was found to be very sensitive to the herbicide Basta through a sensitivity test indicating it could be employed as a selective agent. The bar gene is a practicable and selectable marker gene. The vector containing the expression cassette of the bar gene was transferred to P. subcordiformis by both particle bombardment and glass-bead agitation and transformants were then selected using Basta. Finally, Southern blotting analysis indicated that the bar gene had been successfully integrated into the nuclear genome of P. subcordiformis using both of the transgenic techniques, with the transformation efficiency of the glass-bead method being slightly higher than that of particle bombardment. This is the first report on stable transformation of P. subcordiformis, and will improve fundamental research and enlarge application of this alga. 相似文献
2.
LU Yongzhong ZHANG Xuecheng 《中国海洋大学学报(英文版)》2007,6(3):244-248
In order to investigate the regulation mechanism of the phycocyanin gene,a series of functional analyses of the upstream sequence of cpcB gene from Arthrospira platensis were conducted in E. coli with green fluorescent protein encoding gene(gfp) as the reporter. Results showed that the gfp gene could express at a high level under the promotion of the upstream sequence,suggesting the existence of some strong promoter elements in it. The expression of GFP was influenced by temperature. Higher temperature led to higher expression level. The bioinformatics analyses followed by mutation analyses on the secondary structure of translation ini-tiation region(TIR) revealed that RNA thermosensor might account for the temperature regulation. 相似文献
3.
A harmful algae bloom (HAB) is a dense aggregation of algae in a marine or aquatic environment that can result in significant
environmental problems. To forecast the occurrence of HAB, development of a rapid and precise detection method is urgently
required. In this study, two Skeletonema costatum-like diatoms (SK-1 and SK-2), were identified morphologically under a light microscope, and detected using fluorescent in situ hybridization (FISH). Strain SK-1 was isolated from a frequently HAB affected area of the East China Sea, and strain SK-2
from an aquatic farm in Qingdao, China. Fluorescent DNA probes were designed that were complementary to the ITS sequence (including
5.8S rDNA) of strain SK-1. After hybridization, strong green fluorescence was observed in cells of strain SK-1 under an epifluorescence
microscope; however, no such fluorescence was observed with strain SK-2, which indicates that probes hybridized only the DNA
of the target strain, SK-1, in species-specific manner, and that the two strains do not belong to a same species. This finding
was confirmed by ITS sequence analysis. The FISH technique used in this study was sensitive, simple, and rapid, and is a promising
tool for detecting target HAB species in natural environments. 相似文献
4.
Evaluation and Influence Factor of Green Efficiency of China's Agricultural Innovation from the Perspective of Technical Transformation 总被引：1，自引：0，他引：1
《中国地理科学(英文版)》2021,(2)
Agricultural innovation is important for the green transformation of agriculture. Based on the perspective of technology transformation, this paper builds a theoretical analysis framework and evaluation index system for green efficiency of agricultural innovation,and discusses the evolution laws and influencing factors of the green efficiency of China's agricultural innovation from 2005 to 2017 utilizing the DEA model, Malmquist index, and Tobit regression analysis. The results show that: 1) The overall green efficiency of China's agricultural innovation is not high, the green efficiency of agricultural innovation in eastern China is mainly driven by pure technical efficiency, while that in central and western China is mainly driven by the scale efficiency. The green efficiency of agricultural innovation shows significant spatial differences, and the low efficiency and relatively low-efficiency regions moved to central and southeastern China. 2) Technical progress is the main force affecting the change of green total factor productivity of China's agricultural innovation, seeing a trend of decrease followed by an increase. Pure technical efficiency and scale efficiency exhibit an increasing-decreasing trend, and gradually transform into key factors that restrict the improvement of the green total factor productivity of agricultural innovation. 3) Agricultural technologies' diffusion, absorption, and implementation are three influencing factors of the green efficiency of agricultural innovation. The local level of informatization, the number of agricultural technicians in enterprises and institutions, average education level of residents, and the level of agricultural mechanization have positive impacts on the promotion of the green efficiency of agricultural innovation, promoting the diffusion, absorption and implementation of agricultural innovation technology can significantly improve the green efficiency of agricultural innovation. 相似文献
5.
为了深入了解油气分布规律，利用流体包裹体显微荧光光谱和显微测温以及一维盆地模拟的埋藏热历史，结合地震构造属性和沉积相分析，对泌阳凹陷北部缓坡带核桃园组油气运聚成藏过程进行了研究。结果显示北部缓坡带核桃园组第三段储层检测到橙黄色、黄绿色和蓝绿色3种颜色荧光油包裹体，反映了3幕不同成熟度的原油捕获。油包裹体的丰度和伴生盐水包裹体均一温度和埋藏史曲线表明，北部储层共存在两期三幕充注事件:第一期规模小，发生于断陷末期的相对低成熟度发橙黄色荧光油，第二期发生在中新世构造抬升期的相对高成熟度的黄绿色和蓝绿色荧光油。流体包裹体古压力模拟显示第二期油气充注时储层是超压状态，推测与当时的构造挤压有关。超压持续作用以及北部地层抬升造成与深凹带源岩增加的流体势差为第二期油气提供了强有力的动力；频繁的断层活动，特别是走滑断裂使得单一的砂体顺层输导转变为砂体顺层输导+断裂垂直输导的复合立体运移输导体系，使得原油以高效率运移到凹陷北部缓坡带。这对精细理解构造抬升控制油气大规模运聚具有重要的意义。 相似文献
6.
城市绿地信息在城市研究中的重要作用。但由于各种因素的影响,城市绿地信息提取的精度受到很大的限制,其中,城市中建筑物的阴影是城市绿地信息提取的一个重要限制因素。本研究选取呼和浩特市城区的QuickBird影像,在获取最佳波段组合的基础上,利用多种方法对遥感影像的阴影信息进行提取和消除,以期获得最佳的阴影消除方法,高效地提取城市绿地信息。首先,通过比值运算、波段重组,增强处理影像阴影信息,用最佳指数法分析QuickBird影像阴影提取的最佳波段组合;然后,根据阴影在近红外波段的最小亮度值与最大亮度值的范围建立掩膜,成功提取影像的阴影信息;最后,将色彩空间变换分别与同态滤波和Gamma矫正结合以消除影像阴影,并与其他方法进行对比。研究结果表明,QuickBird影像阴影提取的最佳波段组合为3/4、4、2波段组合,最佳亮度值范围为70-165;色彩空间变换与Gamma矫正相结合的方法可更好地消除阴影,并能较好地保留影像的彩色信息,是消除阴影的最佳方案。 相似文献
7.
Chinese shrimp(Fenneropenaeus chinensis)is an economically important aquaculture species in China.However,cytogenetic and genomic data is limited in the organism partly because the chromosomes are difficult to isolate and analyze.In this study,fluorescence in-situ hybridization(FISH) was used to identify the chromosomes of F.chinensis.The 5S ribosomal RNA gene(rDNA)of F. chinensis was isolated,cloned and then used as a hybridization probe.The results show that the 5S rDNA was located on one pair of homologous chromosomes in F.chinensis.In addition,triploid shrimp were used to evaluate the feasibility of chromosome identification using FISH and to validate the method.It was confirmed that 5S rDNA can be used as a chromosome-specific probe for chromosome identification in F.chinensis.The successful application of FISH in F.chinensis shows that chromosome-specific probes can be developed and this finding will facilitate further research on the chromosomes of penaeid shrimps. 相似文献
8.
This study investigates the contrasting sceneries of community-based tourism after the 2008 Wenchuan earthquake and focuses on the potentials of tourism in transformations towards green economies. How are tourism and environment linked in a sustainable relationship? And how can potentials of community-based tourism be directed in transformations towards green economies? A qualitative research design is used to examine contrasting sceneries and specific green elements of community-based tourism in a Qiang people's fortress village in Mao County, Sichuan Province, West China and further discuss how tourism can be compatible with human wellbeing through demonstrating a commitment to sustainable development. The study argues that potential economic, social and environmental costs of tourism need to be considered when evaluating the loss of community benefits toward sustainability. The green elements suggest the potential of community-based tourism in transformations towards green economies, involving green agricultural, green building, and green energy, green governance, green service and green consumption. This study will contribute to the debate about the relationship between tourism and environment sustainability in the green economies, as well as the critique of what can be learned for community development from green tourism in mountainous regions after the inevitable natural disasters. 相似文献
9.
本文选择大连市沙河口和西岗区为研究区,以Landsat ETM、SPOT等数据为基础,通过遥感解译和反演得到1999年和2013年研究区内绿地及地表温度空间分布及演变特征,结合温度-植被指数（TVX）空间分析法测度城区发展过程中绿色空间减退所引起的热环境效应,以提出更有针对性的城市气候变化适应性规划。研究结果表明：（1）1999-2013年,研究区绿地面积减少43.8%,绿地斑块增加42.9%,城市绿地呈现面积减少、空间分布碎化的演变特征。其中,森林绿地、公园绿地和街区绿地转化为建设用地的贡献率分别为43.8%、42.9%和13.3%,街区绿地因原始面积小且转化率高,被建设用地侵占最为严重。（2）1999-2013年,研究区地表温度最高值上升5.2 K,地表温差增加1.7 K,地温聚集区间由301.6 ~304.7 K上升至306.5~310.9 K,热环境效应加剧。（3）TVX模型追踪结果显示,快速城市化过程中研究区内城市绿地转化为建设用地引起植被覆盖度下降和地表温度上升,其中街区绿地被侵占造成的地表热环境效应大于公园绿地和森林绿地。 相似文献
10.
《山地科学学报》2021,(9)
Rural environmental governance is an important strategy to achieve the goal of building "green liveable villages" in China. However, studies on technology and devices for pollution remediation focus mainly on urban areas; in this regard, few studies have focused on rural areas, especially mountainous or hilly areas with interflow. Coordinate remediation techniques and devices for water-soil restoration in mountain areas(CRWS-mountain), a subproject of the "environmental monitoring and remediation in rural areas" project(ERM-rural project), was undertaken to develop techniques and devices for coordinating the restoration of water and soil in rural mountainous and hilly areas with interflow. The CRWS-mountain project will attempt to explore the mechanisms of pollutant transportation and transformation in the interflow-soil-surface water system, establish in-situ and heterotopic remediation systems in landfill, towns and surrounding areas, and construct a comprehensive system for "polluted soilinterflow-surface water" by integrating all the technologies and devices at Yanting, a typical rural mountainous hilly area in Sichuan, Southwest China. We expect that after using this comprehensive system, the water quality will meet the requirements for local water function zoning. The operation cost is 10% lower than that of the existing urban equipment. 相似文献
11.
12.
A genetic transformation model for the seaweedLaminaria japonica mainly includes the following aspects:
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1.
The method to introduce foreign genes into the kelp,L. japonica
Biolistic bombardment has been proved to be an effective method to bombard foreign DNA through cell walls into intact cells of both sporophytes and gametophytes. The expression ofcat andlacZ was detected in regenerated sporophytes, which suggests that this method could induce random integration of foreign genes.
Promoters to drive gene expression
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2.
The CaMV35S promoter was first used by us to induce the expression of GUS gene in brown algae. But results of further studies suggested that CaMV35S could be a tissue-specific promoter. Our use of SV40 promoter resulted in both transient and stable expression oflacZ andcat in sporophytes or gametophytes. No GUS or LacZ background was found in either sporophytes or gametophytes.The regeneration route of transgenic kelp
The regeneration efficiency of explants is still very low. By using female gametophytes as gene hosts and parthenogenesis as regeneration route, CAT activity and LacZ activity were detected in regenerated sporophytes of parthenogenetic kelp. li]4.|The way to select transgenic kelp
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1.
Results of sensitivity tests showed that kelp was only sensitive to chloramphenicol and hygromycin among many antibiotics. The regenerated sporophytes by parthenogenesis were more sensitive to hygromycin than to chloramphenicol. Resistant kelp was created by transforming female gametophytes with pSV40-CAT and stimulating parthenogenesis followed by selection in medium with lethal concentration of chloramphenicol.
Safety consideration of transgenic kelp
L. japonica was originally introduced from Japan. In China it is a cultured population. The possibility of its negative impact on natural populations is very low. 2) The vectors and target genes used for transformation should be restricted in order to avoid any negative impacts on human health and environment. 3) Specially devised containers (3.6 L, made of 200 μm membrane) were used to ensure that the kelp cannot escape or be eaten by marine animals. 4) To avoid the release of spores, it is very necessary to harvest the kelp at suitable age before the sporangium forms.
13.
A Method for Retrieving Water-leaving Radiance from Landsat TM Image in Taihu Lake, East China 总被引：1，自引：0，他引：1
WANG Deyu FENG Xuezhi MA Ronghua KANG Guoding 《中国地理科学(英文版)》2007,17(4):364-369
The visible and infrared bands of Landsat Thematic Mapper (TM) can be used for inland water studies. A method of retrieving water-leaving radiance from TM image over Taihu Lake in Jiangsu Province of China was investigated in this article. To estimate water-leaving radiance, atmospheric correction was performed in three visible bands of 485nm, 560nm and 660nm. Rayleigh scattering was computed precisely, and the aerosol contribution was estimated by adopting the clear-water-pixels approach. The clear waters were identified by using the Landsat TM middle-infrared band (2.1μm), and the water-leaving radiance of clear water pixels in the green band was estimated by using field data. Aerosol scattering at green band was derived for six points, and interpolated to match the TM image. Assuming the atmospheric correction coefficient was 1.0, the aerosol scattering image at blue and red bands were derived. Based on a simplified atmospheric radiation transfer model, the water-leaving radiance for three visible bands was retrieved. The water-leaving radiance was normalized to make it comparable with that estimated from other remotely sensed data acquired at different times, and under different atmospheric conditions. Additionally, remotely sensed reflectance of water was computed. To evaluate the atmospheric correction method presented in this article, the correlation was analyzed between the corrected remotely sensed data and the measured water parameters based on the retrieval model. The results show that the atmospheric correction method based on the image itself is more effective for the retrieval of water parameters from Landsat TM data than 6S (Second Simulation of the Satellite Signal in the Solar Spectrum) code based on standard atmospheric and aerosol models. 相似文献
14.
A cell line,SHK,was derived from the kidney of spotted halibut Verasper variegates.The cell line was subcultured more than 40 passages in minimum essential medium(MEM)supplemented with fetal bovine serum(FBS)and 10 ng ml-1 basic fibroblast growth factor(bFGF).Cell morphology from primary culture and subculture was observed continuously by microscopy.The SHK cell line consisted predominantly of fibroblast-like cells.The cell line was able to grow between 20℃ and 30℃ with the optimum growth at 24℃ and with a reduced growth between 12℃ and 20℃.The growth rate of the cells increased as the proportion of FBS increased from 10% to 20% at 28℃ with optimum growth at the concentration of 20%.The doubling time of the cells was determined to be 44.8 h.Chromosome analysis revealed that 52% of the SHK cells maintained a normal diploid chromosome number (2n=46).The cells were successfully transfected with green fluorescent protein(GFP)reporter plasmids and the expression of GFP gene in the cells indicated the possible utility of the cells in gene expression studies.The cells were infected by lymphosystis disease virus(LCDV)and found to be susceptible to the virus in cytopathic effect(CPE)observation.The infection was confirmed by PCR and electron microscopy experiments,which proved the existence of the viral particles in the cytoplasm of the virus-infected cells. 相似文献
15.
WANGXing-zhong SUNFeng-hua GUOLiu-jian HEXiao-dong 《中国地理科学(英文版)》2005,15(1):7-15
The quality dimension of the urban living space was addressed much earlier abroad; nevertheless, it has not been studied in the Chinese academia up to date, as evidenced by the limited research literature. Through recourse to Rich BOYER‘s nine-factor theory, this study attempted to evaluate and rank 31 target cities, by using the data from the China Statistical Yearbook 2002. Our research results show that the quality of a city‘s living space is in positive correlation with its size, and that there will be changing demands for the quality of the urban living space as a result of future social, economic, sci-tech, cultural and educational developments, combined with the improvement of the overall quality of urban residents. Previous research was focused on the quality of food, housing, education, transportation and entertainment. However, with the development of the knowledge-economy, such factors as information accessibility, green environment, and air quality, will be major considerations in the quality evaluation of the urban living space. 相似文献
16.
Green tides are caused by the proliferation of chlorophytes under suitable hydrographic conditions.These blooms lead to environmental degradation and negatively impact the waters and seagrass beds,as well as fishing and other recreational activities in the bay.A comprehensive ecological understanding of the bloom dynamics,including the origin and persistence,is needed to foster management decisions.The algae in the great majority of green tide blooms usually belong to two genera of Ulvophyceae,Ulva and Enteromorpha.Ulva has been observed more often in recent years.In China,green tides occurred for the first time in the middle area of the Yellow Sea in 2007,and a large-scale algae blooming broke out in the middle and southern areas of the Yellow Sea in late May 2008.We identified them as Ulva prolifera by comparative analysis of the rDNA internal transcribed spacer 1 (ITS1),5.8S and ITS2 sequences in combination with microscopic observation.Morphological differences were found between the free-floating algae and the attached thalli.Various reproduction patterns of the free-floating algae include sexual,asexual and vegetative propagations,which played important roles in the long-term green tide persistence in China.The ITS sequences of the blooming algae were identical to those of the samples from the Lianyungang sea area but were different from the attached samples from the Qingdao sea area.The results infer that the blooms are originated from other sea areas rather than from the local attached populations. 相似文献
17.
为了阐明喀斯特水库对河流水体碳循环造成的影响, 总结了近年来喀斯特流域筑坝作用对不同形态碳迁移转化和环境影响的研究进展。通过光谱学、稳定及放射性同位素等手段等对喀斯特地区河流-水库系统中的溶解无机碳(DIC)、溶解有机碳(DOC)、颗粒无机碳(PIC)和颗粒有机碳(POC)迁移转化及其控制机制的研究, 发现喀斯特水库碳循环展现出明显的季节性分布特征以及梯级水库群可能会进一步放大单个水库的生态效应, 这一结果不仅极大地促进了人们对水库碳循环的认识, 还有利于探索河流碳循环中"遗失"的碳汇以及更加准确的评估岩溶水库在全球河流碳循环中扮演的角色。喀斯特水库相比非喀斯特水库对人为活动加剧的影响可能具有更强的响应强度, 这也意味着喀斯特水库在全球变暖的趋势中发挥的作用需要得到更准确的评估, 而在未来的研究中, 通过不同分析手段从微观到宏观系统性的总结不同形态碳迁移转化特点将更准确的回答该问题。 相似文献
18.
Chinese shrimp (Fenneropenaeus chinensis) is an economically important aquaculture species in China. However, cytogenetic and genomic data is limited in the organism
partly because the chromosomes are difficult to isolate and analyze. In this study, fluorescence in-situ hybridization (FISH)
was used to identify the chromosomes of F. chinensis. The 5S ribosomal RNA gene (rDNA) of F. chinensis was isolated, cloned and then used as a hybridization probe. The results show that the 5S rDNA was located on one pair of
homologous chromosomes in F. chinensis. In addition, triploid shrimp were used to evaluate the feasibility of chromosome identification using FISH and to validate
the method. It was confirmed that 5S rDNA can be used as a chromosome-specific probe for chromosome identification in F. chinensis. The successful application of FISH in F. chinensis shows that chromosome-specific probes can be developed and this finding will facilitate further research on the chromosomes
of penaeid shrimps. 相似文献
19.
The marine green alga Chaetomorpha valida fouls aquaculture ponds along the coastal cities of Dalian and Rongcheng, China. Unialgal cultures were observed under a microscope to determine the developmental morphological characters of C. valida. Results reveal that gametophytic filaments often produce lateral branches under laboratory culture conditions, suggesting an atypical heteromorphic life cycle of C. valida between unbranched sporophytes and branched gametophytes, which differs from typical isomorphic alternation of Chaetomorpha species. The shape of the basal attachment cell, an important taxonomic character within the genus, was found variable depending on environmental conditions. The 18S rDNA and 28S rDNA regions were used to explore the phylogenetic affinity of the taxa. Inferred trees from 18S rDNA sequences revealed a close relationship between C. valida and Chaetomorpha moniligera. These results would enrich information in general biology and morphological plasticity of C. valida and provided a basis for future identification of green tide forming algae. 相似文献
20.
XIAOYah 《中国地理科学(英文版)》2004,14(2):186-191
The Changbai Mountains is rich in the resources of green food. At present, the low marketization of green food resources in the forest region of the Changbai Mountains becomes the bottleneck to restrict the benign development of its green food industry. With huge market demands at home and abroad, it is the urgent problem how to improve marketization process of green food resources and transfer the resources superiority into the market superiority in the region. According to the investigation, this paper analyzed the status quo and the cause of formation of low-marketization with the method of combining comparative research and practice research. It pointed out that necessary condition of marketization of green food resources in the forest region, such as strategy, economic environment, marketization allocation of sci-tech resources, etc. should be established. Furthermore, the concrete strategies of marketization of green food resources in the region such as market location, strategies of objective markets, combined strategy of marketing, etc. were advanced. 相似文献