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1.
眼斑拟石首鱼肌肉生长抑制素基因克隆及组织表达分析 总被引：1，自引：0，他引：1
肌肉生长抑制素(MSTN)基因对肌肉生长起负调控作用,在动物育种上具有潜在的应用前景.报道了眼斑拟石首鱼(Sciaenops ocellatus)的MSTN基因的序列结构特征及其组织特异性表达.MSTN基因具有3个外显子和2个内含子,编码376个氨基酸.在内含子Ⅰ和3'-UTR区内,分别发现了(TC)4CC(TC)4CT(TC)4和(AC)7微卫星序列.推断的眼斑拟石首鱼的MSTN氨基酸序列具很强的保守性,与石鼓鱼、金鲷、条纹石鮨、白鲈、金眼石鮨之间的相似性在90%以上.在结构上,眼斑拟石首鱼的MSTN包含N端信号序列(1~22氨基酸残基),TGF-β前肽域(41~256氨基酸残基),RXXR蛋白水解位点(264~267位的RARR)和TGF-β区域(282~376氨基酸残基).在检测的10种组织中,MSTN只在眼斑拟石首鱼的肌肉、脑和眼组织中表达,其中在肌肉表达最强. 相似文献
2.
Raft-type wave energy converter (WEC) is a multi-mode wave energy conversion device, using the relative pitch motion to drive its hydraulic power take-off (PTO) units for capturing energy from the ocean waves. The hydraulic PTO unit as its energy conversion module plays a significant role in storing large qualities of energy and making the output power smooth. However, most of the previous investigations on the raft-type WECs treat the hydraulic PTO unit as a linear PTO unit and do not consider the dynamics of the hydraulic circuit and components in their investigations. This paper is related to a two-raft-type WEC consisting of two hinged rafts and a hydraulic PTO unit. The aim of this paper is to make an understanding of the dynamics of the hydraulic PTO unit and how these affect the performance of the two-raft-type WEC. Therefore, a combined hydrodynamic and hydraulic PTO unit model is proposed to investigate and optimize the performance of the two-raft-type WEC; and based on the simulation of the combined model, the relationships between the optimal power capture ability, the optimal magnitude of the hydraulic PTO force and the wave states are numerically revealed. Results show that an approximately square wave type hydraulic PTO force is produced by the hydraulic PTO unit, which causes the performance of the two-raft-type WEC not to be sinusoidal and the energy capturing manner different from that of the device using a linear PTO unit; moreover, there is an optimal magnitude of the hydraulic PTO force for obtaining an optimal power capture ability, which can be achieved by adjusting the parameters of the hydraulic PTO unit; in regular waves, the optimal power capture ability as well as the optimal magnitude of the hydraulic PTO force normalized by the wave height presents little relationship with the wave height, mainly depends on the wave period; in irregular waves, the trends of the optimal power capture ability and the normalized optimal magnitude of the hydraulic PTO force against the peak wave periods at different significant wave heights are generally identical and show a good correlation. All means that the hydraulic PTO unit of the two-raft-type WEC can be tuned to the wave states, and these would provide a valuable guidance for the optimal design of its hydraulic PTO unit. 相似文献
3.
基于上海海洋大学研制的卧式海流能发电装置,在对叶片受力进行分析的基础上,采用雷诺平均N-S方程和滑移网格技术,对不同工况下的叶轮运动进行了数值模拟,获得了力矩系数、能量转换效率及功率等性能参数。通过分析比较不同工况下的各性能参数的变化规律,发现叶轮运动具有周期性,一个周期内的相邻极大、极小值之间相差72°相位角;随着海流速度和叶轮转速的增大,输出功率逐渐增大;而力矩系数和能量利用效率却呈现先增大后减小的趋势,在海流速度v=1.5 m/s,尖速比λ=2.46时,能量转换效率最大,可达到34.6%,输出功率为373 W,并与海试结果进行了对比分析。 相似文献
4.
《Oceanic Engineering, IEEE Journal of》2005,30(3):630-646
Over the last 20 years, there have been tremendous intellectual and technological advancements in the fields of muscle biophysics, biomechanics, and musculoskeletal modeling. These advances have fueled a revolution in integrative muscle physiology. Whereas 20 years ago the notion of understanding the function and design of a muscular system from the molecular to the whole animal level was a dream, it is now becoming a reality. Fish represent an exceptional model for understanding the function and design of the muscular system of vertebrates. There are two fundamental reasons for this preeminence. First, the unique anatomical separation of the different muscle fiber types has made fish the most tractable model (i.e., the use and properties of the different muscle fiber types can be most easily studied). Second, fish utilize the broadest range of movement than any vertebrate. This diversity of movement imposes a wide array of challenges on the muscular system of fish and at the same time enables physiologists/biomechanists to observe how these challenges have been met. These features have permitted us to extract a number of general principles of actuation and control that have evolved over millions of years. Rather than summarizing the considerable literature on fish muscle function and fish swimming, this paper will focus narrowly on a relatively few studies that permit us to extract principles of actuation. These principles may in turn provide some insights into the design and construction of autonomous underwater vehicles (AUVs). 相似文献
5.
氮气浮标是一种新型的剖面浮标,通过增加蓄能器作为被动浮力调节模块,可以利用海洋压差实现更有效率的剖面运动。由于蓄能器的加入,氮气浮标的运动特性相对于常规浮标有所变化。基于一款深海剖面浮标,利用理论分析和运动仿真的方法研究了氮气浮标的运动特性,对氮气浮标主动体积改变量与剖面运动深度之间的对应关系、氮气浮标的剖面运动形式以及氮气浮标的定深悬浮稳定性进行研究。研究表明,氮气浮标只需要主动对浮标体积做较小的改变即可完成同等深度的剖面运动,节省了浮标完成一次剖面运动的能量消耗。但蓄能器的引入增加了浮标完成剖面运动需要的时间,且给浮标的运动带来了突变性和不稳定性。 相似文献
6.
将青岛文昌鱼(Branchiostoma belcheri tsingtauense)肌球蛋白重链基因片段亚克隆到pET-30a质粒,构建出重组表达载体并转化入大肠杆菌Escherichia coli BL21中.经SDS-PAGE和Western杂交检测表明,在IPTG诱导下含有重组载体的菌株可表达分子质量约30 ku的融合蛋白.诱导条件优化实验结果显示,该菌株经0.2 mmol/L IPTG诱导1 h就可大量表达此蛋白.可溶性实验确认该重组蛋白是可溶性蛋白.本研究为文昌鱼肌球蛋白重链特异性抗体的制备奠定了基础. 相似文献
7.
Kern E. Kenyon 《Journal of Oceanography》2004,60(6):1045-1052
Freely propagating surface gravity waves are observed to slow down and to stop at a beach when the bottom has a relatively gentle upward slope toward the shore and the frequency range of the waves covers the most energetic wind waves (sea and swell). Essentially no wave reflection can be seen and the measured reflected energy is very small compared to that transmitted shoreward. One consequence of this is that the flux of the wave’s linear momentum decreases in the direction of wave propagation, which is equivalent to a time rate of change of the momentum. It takes a force to cause the time rate of change of the momentum. Therefore, the bottom exerts a force on the waves in order to decrease the momentum flux. By Newton’s third law (action equals reaction) the waves then impart an equal but opposite force to the bottom. In shallow (but finite) water depths the wave force per unit bottom area is calculated, for normal angle of incidence to the beach, to be directly proportional to the square of the wave amplitude and to the bottom slope and inversely proportional to the mean depth; it is independent of the wave frequency. Constants of proportionality are: 1/4, the fluid density and the acceleration of gravity. Swell attenuation near coasts and some characteristics of sand movement in the near-shore region are not inconsistent with the algebraic structure of the wave force formula. Since the force has a depth variation which is significantly faster than that of the dimensions of the particle orbits in the vertical direction, the bottom induces a torque on the fluid particles that decreases the angular momentum flux of the waves. By an extension of Newton’s third law, the waves also exert an equal but opposite torque on the bottom. And because the bottom force on the waves exists over a horizontal distance, it does work on the waves and decreases their energy flux. Thus, theoretically, the fluxes of energy, angular and linear momentum are not conserved for shoaling surface gravity waves. Mass flux, associated with the Stokes drift, is assumed to be conserved, and the wave frequency is constant for a steady medium. 相似文献
8.
《Ocean & Coastal Management》2000,43(6):445-474
The world's oceans cover three quarters of the global surface and account for about 80% of products taken by humankind from the aquatic systems. The spatial dimensions critical to fishery activities and management have been under constant change for many years because of pressure from two major interdependent forces. The first, of a geopolitical and socio-economic nature, tends to extend national jurisdiction outwards as far as possible from the coast, at the same time fragmenting the ocean area and its jurisdictions into ever-smaller geographical units linked to increasingly localized and decentralized governing institutions. The second, newer force favors increasingly larger geographical management units corresponding as far as possible to a particular harvested ecosystem, i.e. the aggregate of target, associated and dependent species, their productive environment and the exploitation system. This article looks at these two trends and their implications, and examines their convergence in the development of a more modern and responsible form of governance of fisheries. 相似文献
9.
海水贝类养殖对生态系统的影响 总被引：6，自引：0，他引：6
Zhu Mingyuan 《中国海洋大学学报(自然科学版)》2000,30(Z1):53-57
海水贝类养殖在迅速发展的同时 ,也对海洋生态系统产生了影响。这些影响包括由于贝类大量摄食而导致的浮游植物生物量 下降、种类组成改变,以及有机物质和营养盐的截留和再循环从而影响生物地球化学循环, 筏式养殖的“岛屿”效应而引起的水动力的改变。 相似文献
10.
The impact of parameterized topographic internal lee wave drag on the input and output terms in the total mechanical energy budget of a hybrid coordinate high-resolution global ocean general circulation model forced by winds and air-sea buoyancy fluxes is examined here. Wave drag, which parameterizes the generation of internal lee waves arising from geostrophic flow impinging upon rough topography, is included in the prognostic model, ensuring that abyssal currents and stratification in the model are affected by the wave drag.An inline mechanical (kinetic plus gravitational potential) energy budget including four dissipative terms (parameterized topographic internal lee wave drag, quadratic bottom boundary layer drag, vertical eddy viscosity, and horizontal eddy viscosity) demonstrates that wave drag dissipates less energy in the model than a diagnostic (offline) estimate would suggest, due to reductions in both the abyssal currents and stratification. The equator experiences the largest reduction in energy dissipation associated with wave drag in inline versus offline estimates. Quadratic bottom drag is the energy sink most affected globally by the presence of wave drag in the model; other energy sinks are substantially affected locally, but not in their global integrals. It is suggested that wave drag cannot be mimicked by artificially increasing the quadratic bottom drag because the energy dissipation rates associated with bottom drag are not spatially correlated with those associated with wave drag where the latter are small. Additionally, in contrast to bottom drag, wave drag is a non-local energy sink.All four aforementioned dissipative terms contribute substantially to the total energy dissipation rate of about one terawatt. The partial time derivative of potential energy (non-zero since the isopycnal depths have a long adjustment time), the surface advective fluxes of potential energy, the rate of change of potential energy due to diffusive mass fluxes, and the conversion between internal energy and potential energy also play a non-negligible role in the total mechanical energy budget. Reasons for the <10% total mechanical energy budget imbalance are discussed. 相似文献
11.
潮流能发电装置支撑结构对水轮机水动力学性能影响研究 总被引：1，自引：0，他引：1
水平轴潮流能水轮机在工作过程中,由于支撑结构的存在,会使水轮机周围流场中的潮流流向、流速等参数发生不同程度的改变,进而影响水轮机的性能和发电装置的稳定性。为了研究支撑结构对水轮机水动力学性能的影响规律,以某100 k W单立柱座底式潮流能发电装置的支撑结构为研究对象,采用CFD方法,分别在正、反向来流时采用不同支撑结构的共六种工况下,对潮流能水轮机模型的获能和受力进行数值模拟。通过水槽模型试验,验证数值模拟的可靠性。研究结果表明:支撑结构对水轮机的水动力学性能的影响不容忽视,针对所研究的支撑结构,在正向来流时水轮机的获能系数降幅约30%,轴向力系数降幅约28%;反向来流时的降幅更大,分别约为63%和41%。 相似文献
12.
以凡纳滨对虾(Litopnnaus vannamei)幼虾为实验动物,在其基础饲料中分别添加芽孢杆菌(Bacillus sp.)胞外蛋白粗提物(1.0%,胞外蛋白组)、胞内多糖粗提物(1.0%,胞内多糖组),以基础饲料为对照组饲料,进行为期28d的养殖实验,探讨芽孢杆菌胞外蛋白和胞内多糖粗提物对幼虾生长、能量代谢及抗病能力的影响。研究表明,芽孢杆菌2种粗提物对凡纳滨对虾的生长和成活率影响不显著(P>0.05)。饲喂2种粗提物影响了凡纳滨对虾肝胰腺和肌肉中能量代谢相关酶活力。胞外蛋白组肝胰腺中磷酸果糖激酶(PFK)、乳酸脱氢酶(LDH)活力在养殖实验前期显著高于对照组(P<0.05),而肌肉中PFK、LDH活力显著低于对照组(P<0.05);胞内多糖组肝胰腺中己糖激酶(HK)活力及肌肉中PFK、LDH活力显著低于对照组(P<0.05)。饲喂2种粗提物均显著提高了凡纳滨对虾肌肉中琥珀酸脱氢酶(SDH)活力和肝胰腺中电子传递系统(ETS)活力(P<0.05),胞外蛋白粗提物对肝胰腺中的SDH活力也有显著的提高作用(P<0.05)。饲喂2种粗提物显著降低了凡纳滨对虾肝胰腺及肌肉组织中脂肪酸合酶(FAS)含量(P<0.05)。此外,饲喂2种粗提物均会显著提高凡纳滨对虾肠道蛋白酶活力(P<0.05),而胞外蛋白粗提物使凡纳滨对虾肠道脂肪酶活力显著降低(P<0.05)。白斑综合征病毒(WSSV)感染后,与对照组相比,胞外蛋白组和胞内多糖组凡纳滨对虾半致死时间分别延长了26.19%和7.14%。根据以上结果推测,芽苞杆菌2种粗提物能够影响凡纳滨对虾能量代谢水平并提高其抗WSSV能力,且二者之间密切相关。 相似文献
13.
14.
Nature evolved an elegant actuating system, muscle, which can be controlled by a relatively small molecule, myosin. We briefly introduce herein an artificial approach based on applying molecular actuating building blocks to mimic the function of muscle. A particular focus of this review is the design of a molecular hinge. Designed molecular mechanisms have great potential to produce quick responses and large deformations. 相似文献
15.
为解决海洋监测微型传感器供能问题,设计新型波浪能捕获装置,在海面振荡浮筒气室产生空气气柱,驱动介电弹性体形变发电为传感器供能。建立振荡浮子式气柱数值模型,研究新型振荡水柱发电计算理论。利用水动力仿真软件AQWA求解浮子所受波浪力作用振荡幅值、辐射阻尼和附加质量。基于Simulink软件分别计算波浪作用下浮子位移和气室内水柱位移,根据两者的位移差计算气室体积变化所产生的空气压强、介电弹性体发电薄膜形变量和系统输出电能,单次循环周期最大发电量达到24.6 mJ。分析波浪周期、发电薄膜几何参数等对输出电能的影响。 相似文献
16.
大黄鱼肌肉组织cDNA全长文库的构建及EST分析 总被引：1，自引：0，他引：1
以大黄鱼肌肉组织为材料,利用Creator Smart cDNA Library Construction Kit构建了大黄鱼肌肉组织的全长cDNA文库.文库质量分析表明：cDNA文库容量不低于1.68×106cfu/mm3,重组率达96%,插入片断的平均长度大于1 000bp.随机挑取512个cDNA克隆进行5’端测序,其中430条ESTs的长度大于100bp;并初步拼接得到了230个单基因簇（unigene）,其中包括58个重叠群（contigs）,172个单拷贝ESTs（singletons）,冗余度为46.51%.经检索,35个ESTs在BLASTx上无明显的同源性（E值≤1.00×10-10）,为新基因.195个ESTs与已报道的基因有较高的同源性;其中与能量相关基因的ESTs丰度最高,占总数的20.00%.蛋白质合成、细胞骨架和信号传导次之,比例分别为13.48%、12.17%、10.00%,其中包括高迁移率族蛋白B1、瘦素受体、β1-热激蛋白等.这些EST数据为进一步筛选和克隆大黄鱼肌肉特异性表达基因提供了平台. 相似文献
17.
We presented a detailed investigation on the movement of two-headed Brownian motors in an asymmetric potential under a feedback control. By numerical simulations the direct current is obtained. The current is periodic in the initial length of spring. There is an optimal value of the spring constant. And the dependence of the current on the opposing force is reversed. Then we found that when the change of the temperature and the opposing force have optimal values, the Brownian motors can also obtain the optimal efficiency. 相似文献
18.
Increasing offshore oil and gas activities in the European Arctic has raised concerns of the potential anthropogenic impact of oil-related compounds on the polar marine ecosystem. We measured cellular energy allocation (CEA) in the sea ice amphipod Gammarus wilkitzkii after exposure for one month to the water soluble fraction (WSF) of oil. The CEA biomarker measures the energy budget of organisms by biochemically assessing changes in carbohydrates, protein and lipid content as well as the electron transport system activity. A significantly higher protein content was observed in the medium dose compared to controls, while the total energy budget was not affected in G. wilkitzkii. This indicates that parts of the energy budget of G. wilkitzkii, which is a key species in the Arctic ecosystem, is affected by a WSF of oil. 相似文献
19.
Maneuvers may be defined as a series of changes in direction and position for a specified purpose (as in changing course, switching tracks, or docking). Although the definition excludes hovering, this is considered to be a maneuver by biologists. Maneuvers are typically complex, combining more than one translational and rotational component. They are usually driven by hydrodynamic forces, which may be classified as trimming (steering), driven by the kinetic energy of a moving animal, or powered forces, driven by force generation by the control surfaces themselves. Force production by effectors are affected by molecular structure, material properties, degrees of freedom for movement, the number of propulsors working together, and interactions with other organisms/objects, as well as time through a maneuver and/or a powered fin beat. The result is a large repertoire of maneuvering behaviors and high redundancy in control systems. There is no unanimity on quantifying maneuvering performance, but maximum capabilities appear to be most appropriate. Performance metrics will vary from maneuver to maneuver and the scaling product group seems unlikely even for specified maneuvers. An exception is relative volume swept out. Seeking inspiration from biology for the design of autonomous underwater vehicles requires explicit a priori statements of mission goals. 相似文献
20.
《中国海洋工程》2021,(3)
In the present study, the dynamics of the tendon system of a tension-leg platform(TLP) is investigated through the absolute nodal coordinate formulation(ANCF). Based on the energy conversion principle, the stiffness, generalized elastic force, external load and mass matrices of the element are deduced to perform the element assembling by using the finite element method. Then the motion equation of the tendon/riser is established. In this study, the TLP in the International Ship Structures Committee(ISSC) model under the first and second wave forces is considered as the case study. The simulation is performed in the MATLAB environment. Moreover, the accuracy and reliability of the programs are verified for cases of beam model with theoretical solutions. It is found that the motion response of tendons is affected by the TLP movement and environmental load, simultaneously. Then, the motion response is calculated using the SESAM software and exported as the boundary of ANCF tendons. Finally, the static and dynamic characteristics of the four tendons of ISSC TLP are analyzed systematically by the ANCF method.Performed analysis proves the effectiveness and feasibility of the ANCF method. It is concluded that the proposed method is a powerful scheme for calculating the dynamics of tendon/riser in the field of ocean engineering. 相似文献