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1.
本研究选取5种海洋动植物大分子高聚物或其天然组织,包括虾壳、鱼鳞、海带叶片、几丁质和壳聚糖,分别在海水表层和沉积物环境中进行富集,定期取样,通过高通量测序分析菌群多样性。结果发现,不同有机物原位富集的细菌多样性存在较大的差异,而且同种底物在海水表层与沉积物中的降解菌菌群差异较大。从物种多样性看,在海水表层环境中富集的鱼鳞样品种群最丰富,而沉积物环境中富集的海带叶片样品菌群多样性最低,除其优势菌群为热袍菌门外,其他所有富集物中优势菌均为变形菌门。其中脱硫杆菌科、黄杆菌科、脱硫弧菌科和弧菌科占有较大比例,脱硫杆菌科在所有样品中优势较大,黄杆菌科在海水表层环境样品中为优势菌群,弧菌科在沉积物样品几丁质和壳聚糖样品中占比较高。本研究通过对同种海洋环境中不同富集样品之间以及同种底物不同环境中富集菌群之间的比较,分析结果得到了原位条件下参与大分子聚合物降解的菌群种类,但有待于在更多不同的海域富集物中进行验证。 相似文献
2.
Michael J. Cruickshank 《Marine Georesources & Geotechnology》2013,31(1):85-86
Abstract The engineering properties of deep continental margin sediments were determined on a worldwide basis. Deep Sea Drilling Project (DSDP) core data and material were utilized from over 900 cores obtained from 89 sites, primarily on the continental margins. Cores were recovered from penetrations to 200 m in water depths averaging 3000 m. Supplementary laboratory testing on selected cores was directed toward determining index properties and shear strength properties of the sediments. The study included a literature review of deep‐sea soil properties, the results of which are to be used by DSDP to evaluate foundation conditions for reentry cones with long casing strings. The results will also be used for a feasibility study of an ultradeepwater marine riser and well‐control system. The marine sediments examined can be divided into three main types: clay, calcareous ooze, and siliceous ooze. Sediment distribution consisted of 48% calcareous ooze, 43% clay, 6% siliceous ooze, and 3% volcanic ash. Because of the sample disturbance inherent in the deepwater coring operation, emphasis was placed on analyzing sediment properties not significantly affected by changes in in situ stresses and structure caused by sampling. Averages and ranges in values of water content and unit weight are presented for the three main sediment types. Plasticity and strength characteristics are discussed in detail and the elastic and compression properties are outlined. The geotechnical properties of deep continental margin soils are summarized. 相似文献
3.
Mangrove sediments play a pivotal role in the nutrient biogeochemical processes by behaving as both source and sink for nutrients and other materials. Surface and core sediments were collected from various locations of the Pichavaram mangrove (India) and analyzed for grain size distribution, nutrients and stable N isotope (δ15N) signatures in order to understand the spatial and vertical distribution of nutrients and biogeochemical processes of the C, N, P and S in this ecosystem. Sand is the dominant fraction followed by silt and clay. Spatial distribution of nutrients is controlled by the external and internal loadings, whereas vertical distribution is largely driven by the in situ microbial activities. Interior mangrove sediments contain higher concentrations of organic carbon (OC) than the estuarine sediments reflecting high rates of organic matter retention. Finer fractions of sediment hold ∼60% OC due to high surface area. At some sampling points, moderately high δ15N signatures were observed and this may be because of agricultural runoff and aquaculture effluents. 相似文献
4.
Gravity core samples provide the basic data source for a wide range of geological, geotechnical and geochemical studies. However, the length of the core recovered is often less than the penetration achieved by the corer, such cores being described as shortened. If the penetration of the corer has been measured, and it is assumed that no dropout of the core occurs as the barrel is withdrawn from the seabed, it is present practice to reconstruct in situ dimensions using an overall correction factor based on this penetration and the length of core recovered. However, measurements, reported here, have been made of corer penetration and sample entry and these show that the entry deficit (penetration minus sample entry) develops in some instances continuously, and in others intermittently. These results indicate that an overall correction factor is unlikely to be appropriate to any given section of the core. 相似文献
5.
Takashi Ishimaru Hirotaka Otobe Toshiro Saino Hiroshi Hasumoto Toshisuke Nakai 《Journal of Oceanography》1984,40(3):207-212
A realtime monitoring system for observing physical, biological, and optical oceanographic parameters in shallow waters was developed. It consists of a CTD with oxygen sensor, anin situ fluorometer, anin situ transmissometer, and anin situ quanta meter together with an on-deck quanta meter. The system is also equipped with a water sampling device. Water sampling can be done during the upward cast on the basis of depth profiles of temperature, salinity, oxygen,in situ fluorescence, transmittance, and underwater irradiance taken during the downward cast. 相似文献
6.
Currently, two major types of ship-operated underwater instruments are used for heat flow measurements including a Lister-type
heat probe (LTHP) and small temperature loggers (STLs) that are attached to a solid-steel lance or a core barrel. In both
operations, penetration friction heat introduces a transient disturbance to the temperature of the surrounding sediments.
A pragmatic approach is to extrapolate a cylindrical temperature decay function to estimate the equilibrium ambient temperature
(EAT) and equilibrium ambient temperature gradient (EATG) of the sediments from short temperature recordings. The extrapolated
EAT and EATG will greatly affect the estimate of the base of the gas hydrate stability zone (BGHS). In order to achieve a
better extrapolation of EAT, EATG, and 1-s temperature sampling requirements, compact (22.2 cm × 2.2 cm) high-resolution temperature
loggers (CHTLs) have been designed to work with a sediment core barrel. The mechanical and electronic design of the CHTL is
detailed in the text. With a 24-bit, low noise A/D converter embedded in the mix-signal microprocessor, including a highly
stable reference resistor based ratiometric scheme, the CHTL is capable of resolving 0.1 m°C in the range of −1 to 25°C. It
has a memory capacity of 4 Mbyte which can work continually up to 16 days with a 1-s sampling interval. From a data processing
efficiency consideration, field experiments indicate that adopting short support fins to attach the CHTLs to a relatively
small size core barrel is better than using high support fins. A similar approach such as extrapolating the cylindrical temperature
decay function to estimate EAT, EATG can be obtained from regressing a direct calculated temperature gradient of short recorded
data. The resulting EATG accuracy may be significantly improved through the application of the proposed correction formula
and therefore is much better than that which is directly calculated from the extrapolated EATs. 相似文献
7.
This study investigated differences in the community structure and environmental responses of the bacterial community in sediments of the Bohai Sea.Illumina high-throughput sequencing technology and real-time PCR were used to assay the bacterial 16S rRNA genes in the surface sediments of 13 sampling stations in the Bohai Sea.The results showed that sediments at the majority of the 13 sampling stations were contaminated by heavy metal mercury.The main phyla of bacteria recorded included Proteobacteria(52.92%),Bacteroidetes(11.76%),Planctomycetes(7.39%),Acidobacteria(6.53%)and Chloroflexi(4.97%).The genus with the highest relative abundance was Desulfobulbus(4.99%),which was the dominant genus at most sampling stations,followed by Lutimonas and Halioglobus.The main factors influencing bacterial community structure were total organic carbon,followed by depth and total phosphorus.The content of lead,cadmium,chromium,copper and zinc had a consistent effect on community structure.Arsenic showed a negative correlation with bacterial community structure in most samples,while the impact of mercury on community structure was not significant.The bacterial community in sediment samples from the Bohai Sea was rich in diversity and displayed an increase in diversity from high to low latitudes.The data indicated that the Bohai Sea had abundant microbial resources and was rich in bacteria with the potential to metabolize many types of pollutants. 相似文献
8.
Abstract A differential piezometer was used to monitor excess pore pressure in the soft clayey seafloor sediments of Block 28, South Pass, Mississippi delta, from September 1975 to March 1976. An ambient excess pore pressure of about 32 kPa was measured at a depth of 6.4 m below the mudline in a water depth of 19 m. Storm‐wave‐generated cyclic fluctuations of ± 4 kPa about the ambient were measured during Hurricane Eloise. Irregular, long‐period, small‐amplitude fluctuations in excess pore pressures persisted for 4 days following the storm. An effective stress analysis was made by using excess pore pressures; in situ field vane‐shear strength, t fv, measurements; and laboratory wet unit weights measured by Lehigh and NOAA. The effective stress of the SEA‐SWAB site soil was calculated to be zero to a depth of about 6 m, below which it increased to 3.5 kPa at a depth of 15 m. Values of c´ = 4.6 kPa, ?´ = 56°, and T FV/σvo(c/p) =0.1–0.2 were calculated, and it was concluded that these data do not represent the in situ condition of the soil because of the probability that the measured soil properties were affected by the presence of gas. However, it is clear that the soil is significantly underconsolidated. 相似文献
9.
Naomi Harada Nobuhiko Handa Tadamichi Oba Hiromi Matsuoka Katsunori Kimoto Masashi Kusakabe 《Journal of Oceanography》1997,53(1):1-7
The ages of fossil planktonic foraminifera,Pulleniatina obliquiloculata, in sediments (core 3bPC) from the western North Pacific were determined by aspartic acid chronology, which uses the racemization
reaction rate constant of aspartic acid (kAsp). Aspartic acid racemization-based ages (Asp ages) ranged from 7,600 yrBP at the surface, to 307,000 yrBP at a depth of 352.9
cm in the sediments. This sediment core was also dated by the glacial-interglacial fluctuation of δ18O chronology, and the ages determined by both chronologies were compared. The ages derived from aspartic acid chronology and
δ18O stratigraphy were more or less consistent, but there appeared to be some differences in age estimates between these two
dating methods at some depths within the core. In the core top sediments, the likely cause for the age discrepancy could be
the loss of the surface sediment during sampling of the core. At depths of 66.3 and 139 cm within the core, Asp ages indicated
reduced sedimentation rates duringca. 60,000-80,000 yrBP andca. 140,000–190,000 yrBP. The maximum age differences in both chronologies are 33,000 yr and 46,600 yr during each of these periods.
These anomalous reductions in sedimentation rates occurring during these periods could possibly be related to some geological
events, such as an increased dissolution effect of the calcium carbonate in the western North Pacific. Another possible reason
for these age differences could be the unreliability in δ18O ages of core 3bPC as they were estimated by δ18O ages of another core, 3aPC. 相似文献
10.
Abstract The advanced piston cover (APC) has been used by the Ocean Drilling Program since 1985 for recovering soft sediments from the ocean floor. The pullout force measured on extracting the core barrel from the sediment is shown to correlate with the average shear strength of the sediment core measured in the ship's laboratory. A simple rule of thumb is derived relating the shear strength of the sediment to the pullout force. Multiple APC holes at individual sites allow the consistency of the pullout measurements to be assessed. The effects of different operational procedures during APC coring are also explored. Although generally applicable, the correlation between pullout force and laboratory measurements of shear strength breaks down for some APC holes, possibly because of the disturbance of some sediment types during the APC coring process. A better understanding of the physical process of APC coring, and its effect on the properties of the sediment both inside and immediately outside the core barrel, would indicate what confidence can be put on the measurement of pullout force as a way of evaluating the in situ shear strength of deep sea sediments. 相似文献
11.
Gil Young Kim Gwang Soo Lee Dong Geun Yoo Young Kyo Seo Im Hak Kim 《Marine Georesources & Geotechnology》2018,36(3):264-270
The KISAP (Korea Institute of Geoscience and Mineral Resources Seafloor Acoustic Probe) was developed to obtain in situ compressional wave velocity and attenuation profiles for seafloor sediments. The instrument comprises recording channel receivers, and an acoustic source, and can be attached to a corer or a probe. Here, we report experiments comparing KISAP in situ velocity to laboratory velocity of colocated piston cores. KISAP data matched laboratory data (corrected for temperature and pressure) reasonably. KISAP can be used to collect in situ acoustic data and can be effectively used to calibrate previous laboratory data to in situ data. 相似文献
12.
Pore pressures in marine sediments: An overview of measurement techniques and some geological and engineering applications 总被引：1，自引：0，他引：1
P. J. Schultheiss 《Marine Geophysical Researches》1990,12(1-2):153-168
Pore pressures in the seabed are extremely sensitive to any imposed stress because of the low permeabilities commonly exhibited by marine sediments. Consequently, the measurement of sediment pore pressures can be used to infer either the nature of the imposed stress (if the sediment properties are known) or the physical properties of the sediment (if the imposed stresses are known). Stresses of many different types may be exerted on the seabed either through hydrostatic forces (e.g. tidal and wave effects), or directly by lithospheric forces (e.g. tectonic and thermal forces). Several techniques for measuring in situ pore pressures in the upper few metres of sediments have been developed, and one instrument, the PUPPI, will operate autonomously in water depths up to 6000 m. Basic sediment properties and processes can already be inferred from pore pressure responses using this technique. However, further application and development could greatly enhance its capability, especially for long-term monitoring of sediment conditions. In this Chapter, pore pressure measurement techniques are briefly reviewed and problems are highlighted. An outline is given of some of the many ways in which pore pressure measurements could be used to gain further insight into geological processes and to determine some of the pertinent sediment properties more accurately for engineering applications. 相似文献
13.
用原子吸收光谱法(AAS)和原子荧光光度法(AFS)测定了采集于北海银滩镇、厦门海门岛、宁德漳湾镇、乐清西门岛、温岭坞根镇、三门花桥镇等地区自然滩涂以及温岭坞根镇养殖区的可口革囊星虫(Phascolosoma esculentas)及其栖息地的底泥样品中Zn、Cu、Mn、Fe、Cd、Pb、Hg 和As 等8 种重金属含量, 并对检测结果进行了含量特征、生物体与栖息地底泥中重金属含量相关性分析、生物体重金属富集系数分析。结果表明: 不同栖息地可口革囊星虫间的重金属含量差异性显著(P<0.05), 各群体中Pb 含量均超过标准, 厦门海门岛群体中Cd 含量超标; 厦门海门岛、乐清西门岛自然滩涂栖息地和温岭坞根镇养殖区等底泥中重金属含量相对较高, 所有采样点底泥中Cu 含量以及乐清西门岛自然滩涂底泥中Cd 含量达到沉积物质量标准Ⅱ类; 可口革囊星虫对Zn、Cu、Fe 的富集系数均小于1, 对Mn、Cd、Pb、Hg 和As 的富集系数均大于1。Zn、Cu、Fe、Pb 和As 的富集系数在大部分群体间差异性较小; 可口革囊星虫中的Fe 和As 含量与底泥中的Fe 和As 含量高度正相关, Zn 和Hg 含量的相关性次之, 其余重金属元素含量的相关性较差。 相似文献
14.
The magnetostratigraphy of sediments drilled at two sites (998 and 1001) in the Caribbean Sea during the Ocean Drilling Program (ODP) Leg 165 is established directly from downhole measurements. For the first time in the ODP, the magnetostratigraphy of sediments is determined from both logging magnetic data and paleomagnetic analysis of core material for an age interval of about 16 m.y. across the Cretaceous/Tertiary boundary. The total magnetic induction and the susceptibility of the surrounding sediment were recorded in situ and continuously by the GHMT (Geological High-sensitivity Magnetic Tool). The aim of processing these data is to determine the magnetic polarity. At Site 998 (Cayman Rise), no paleomagnetic results are available from the recovered cores given a hard secondary overprint. Nonetheless, a magnetostratigraphic sequence from early Miocene to late Oligocene is proposed from the analysis of the GHMT data. The comparison with biostratigraphic results shows the accuracy of the resulting magnetic polarity. At Site 1001 (Hess escarpment), cores were recovered in two holes thus allowing the comparison of the magnetostratigraphic results obtained from the GHMT data and from paleomagnetic analysis of core samples over a length of 200 m representing about 16 m.y. from early Eocene to late Cretaceous times. The sedimentation rates computed from the determined magnetochrons are very similar and correspond to the ones obtained from shipboard biostratigraphic results. One of the most striking result obtained from the magnetostratigraphic results, especially the GHMT data, at Site 1001, is the abrupt decrease in the sedimentation rate across the K/T boundary. This suppressed sedimentation lasted at least 4 Ma after the K/T event. 相似文献
15.
Sediment disturbance associated with the Deep Sea Drilling Project coring and core‐handling program are discussed along with the effects of sample disturbance on some measurements of physical properties. Information was obtained from shopboard observations and testing on Leg 43 and shore‐based testing of several sediment samples. Disturbance results from (1) remolding of the particle structure during coring, and (2) swelling induced by stress release and thermal expansion in the presence of excess water. Remolding is most significant to depth of 50–100 m, whereas swelling effects increase with coring depth. As a result, the shipboard data are not rep‐sentative of in situ sediment properties. The tendency is for porosity to increase and for bulk density to decrease with increased swelling. Strength is significantly reduced for swelled samples with further reductions from remolding. All observations and data suggest a need for improved coring capabilities and an in situ testing program. 相似文献
16.
Mud samples were collected from the eastern coast of Osaka Bay from April 1984 to August 1985 and were incubated atin situ mud temperatures to examine the effects of temperature on the liberation of seven species of red-tide algae.
Skeletonema costatum appeared extensively over a wide temperature range of 9–25°C. The percentage of liberation of benthicHeterosigma akashiwo cells tended to decrease with decreasing temperature, but this species also showed high percentages of liberation (40–100 %) in April whenin situ mud temperatures were still low (9.5–10°C). Germination of benthicHeterocapsa triquetra cells was active at temperatures lower than 10°C but was inhibited to below 20% at temperatures higher than 21°C. This indicates that the supply of vegetativeHeterocapsa cells from bottom muds to seawaters becomes inactive from summer to early autumn. The optimum temperature range for the liberation of benthicChattonella sp. cells was 16–21°C. ForProrocentrum micans, Prorocentrum minimum, andProrocentrum triestinum, no vegetative cells were released from bottom mud at any of the temperatures examined (9–25°C).Field observations on the population dynamics of vegetative cells conducted from April to September 1984 revealed that a close link exists between liberation ofS. costatum andH. triquetra and their blooms in the waters of Osaka Bay. On the basis of these findings, the ecological role of benthic cells in initiating red tides in Osaka Bay is discussed. 相似文献
17.
18.
Black spots produced by buried macroalgae in intertidal sandy sediments of the Wadden Sea: Effects on the meiobenthos 总被引：3，自引：0，他引：3
The effects of buried decaying macroalgae on meiobenthos were examined in intertidal sandy sediments of the Wadden Sea of Lower Saxony. In situ experiments confirmed that one of the principal causes of the formation of reduced surface sediments or ‘black spots’ on the tidal flats is the increasing occurrence and subsequent decomposition of filamentous green algae (Enteromorpha spp.) buried in the sediment. Five to fifteen days after algal material had been buried, the sediment surface turned black. The impact of these black spots on meiobenthos was dramatic: the changed chemical conditions in the sediment resulted in long and drastic reductions in meiofaunal abundance and number of taxa. A multi-dimensional scaling (MDS) analysis of data on meiobenthic abundances revealed that samples from black-spot areas were clearly separated from those of control and reference areas. Re-oxidized black spots showed recolonization by meiofaunal animals, with numbers of individuals and taxa similar to those of oxidized surface sediments. The use of abundances of members of higher meiobenthic taxa to monitor changes in the sediment's chemistry, especially those caused by biomass overload, is discussed. 相似文献
19.
Although environmental factors such as grain size and organic carbon content may influence the distribution of microbes in marine sediments, there has been little experimental study of the topic to date. To investigate how those sediment variables affect microbial colonisation under in situ conditions, deep‐sea sediments and artificial sediments (glass beads, sands) were incubated in the Arctic deep sea at 2500 m water depth with or without chitin, one of the most important carbon polymers in marine environments. Microbial abundance, biomass, chitobiase activity and changes in community structure were monitored after 7 days and 1 year. In control sediments without chitin addition, no significant changes in microbial abundance, biomass and activity were observed after 1 year. In the presence of chitin, however, considerable increases in these parameters were recorded in all three sediment types tested. Regardless of chitin addition, natural deep‐sea sediments were always associated with higher values of microbial abundance, biomass and activity compared with artificial sediments. Sediment type was always found to be the most significant factor explaining variation in enzymatic activity and bacterial community structure as compared to the effects of chitin amount, incubation time, and changes in cell number or biomass. Overall, this is the first in situ study that has addressed the effects of multiple factors and their interactions on abundance, biomass, activity and community structure of microbial communities in the deep Arctic Ocean. 相似文献
20.
Molecular data have shown that jellyfishes are more geographically restricted and evolutionarily divergent than previously thought. We examined genetic variation and divergence within the meroplanktonic barrel jellyfish Rhizostoma pulmo in the Mediterranean Sea; specific sampling areas were the northern Adriatic, western Mediterranean and Tunisian coast. A total of 19 sampling sites and 68 sequences of the mtDNA cytochrome c oxidase subunit I (COI) gene were used. Of the 68 COI sequences, 45 were newly collected specimens which originated from nine sampling sites along the Tunisian coast. A total of 24 haplotypes were obtained and the specimens sampled were characterised by relatively high levels of haplotype diversity (h = 0.866) and low levels of nucleotide diversity (π = 0.004). Haplotype network analysis showed the presence of three distinct phylogenetic lineages (populations), with separate geographic ranges in the northern Adriatic, western Mediterranean and Tunisian coast. The observed genetic differentiation between these three lineages was supported by the presence of significant genetic differentiation between the 19 populations (FST = 0.757, p < 0.001). The high level of genetic differentiation detected in the barrel jellyfish investigated could be attributed to either intrinsic and/or extrinsic barriers to genetic exchange between different populations that may have adapted to different environmental conditions. 相似文献