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1.
Experiments were carried out to investigate the potential for bacterial colonization of different substrates in karst aquifers and the nature of the colonizing bacteria. Laboratory batch experiments were performed using limestone and PVC as substrates, a natural bacterial isolate and a known laboratory strain (Escherichia coli [E. coli]) as inocula, and karst ground water and a synthetic formula as growth media. In parallel, fragments of limestone and granite were submerged in boreholes penetrating two karst aquifers for more than one year; the boreholes are periodically contaminated by enteric bacteria from waste water. Once a month, rock samples were removed and the colonizing bacteria quantified and identified. The batch experiments demonstrated that the natural isolate and E. coli both readily colonized limestone surfaces using karst ground water as the growth medium. In contrast, bacterial colonization of both the limestone and granite substrates, when submerged in the karst, was less intense. More than 300 bacterial strains were isolated over the period sampled, but no temporal pattern in colonization was seen as far as strain, and colonization by E. coli was notably absent, although strains of Salmonella and Citrobacter were each observed once. Samples suspended in boreholes penetrating highly fractured zones were less densely colonized than those in the borehole penetrating a less fractured zone. The results suggest that contamination of karst aquifers by enteric bacteria is unlikely to be persistent. We hypothesize that this may be a result of the high flow velocities found in karst conduits, and of predation of colonizing bacteria by autochthonous zooplankton. 相似文献
2.
Occurrence and distribution antibiotic resistance of heterotrophic bacteria isolated from a marine beach 总被引：5，自引：0，他引：5
Mudryk ZJ 《Marine pollution bulletin》2005,50(1):80-86
Antibiotic resistance of heterotrophic bacteria isolated from a sandy beach in Sopot, at the Southern Baltic Sea coast was determined. The levels of resistance of bacteria to various antibiotics differed considerably. Bacteria inhabiting the middle part of the beach and the dune were most resistant; the least resistant were bacteria isolated from the sea-beach contact zone. Generally, there were no significant differences in antibiotic resistance between pigmented and non-pigmented bacteria. Bacteria isolated from the surface layer of the sand were more resistant to the tested antibiotics than bacteria from the subsurface layers. The majority of bacterial strains were resistant to 3-8 antibiotics. Bacterial resistance to antibiotics was dependent on their chemical structure. 相似文献
3.
David Muscalo 《Ground Water Monitoring & Remediation》2004,24(1):81-89
The New Jersey Department of Environmental Protection's Technical Regulations require the horizontal and vertical delineation of contamination. Monitor wells screened at increasingly deeper intervals are used to delineate vertical contamination. In New Jersey, the open interval in a bedrock well cannot exceed 7.6 m. Since contamination has been found at depths as great as 91.4 m in a production well in the study area, it would be prohibitively expensive to install monitor wells with 7.6 m open holes at ever-increasing depths until no contamination was found. Isolation of discrete zones in boreholes using pneumatic packers was implemented at a site in north central New Jersey. Ground water samples were collected from selected 6.1 m sections of boreholes drilled into fractured bedrock at three locations on the property and one offsite location. The ground water samples were analyzed in a field laboratory. The analytical results were used to determine the vertical extent of gasoline-related compounds dissolved in the ground water on the property and offsite. These compounds include benzene, ethylbenzene, methyl tertiary butyl ether, toluene, and xylenes. The four boreholes were converted into bedrock monitor wells. The intake interval for each of the wells was selected through evaluation of the vertical distribution of contaminants as determined from analytical results obtained from a field laboratory located onsite. Three wells are used for the recovery of contaminated ground water. The recovered water will be treated at the onsite air-stripping unit. The fourth well is used to chemically and hydraulically monitor the progress of the ground water recovery program. 相似文献
4.
Uptake of Dissolved and Oil Phase Organic Chemicals by Bacteria 总被引：1，自引：0，他引：1
James R. Mihelcic Annette Pritschow Donald R. Lueking 《Ground Water Monitoring & Remediation》1995,15(3):100-106
Hydrophobic organic chemicals (HOCs) discharged into soil and ground water will partition into gaseous, aqueous, oil, and sorbed phases. Knowledge of how bacteria assimilate HOCs is important to individuals involved in evaluating intrinsic, or engineered, bioremediation. The majority of bacteria isolated from the subsurface are gram-negative. The outer membrane of gram-negative organisms acts as a selective barrier to many solutes, including hydrophobic chemicals. Thus, diffusional transport of a hydrophobic solute through the outer membrane may be the rate-limiting step in biodegradation. Bacteria may also produce biosurfactants that can facilitate cell-oil contact or assist solubilization of oil and sorbed phases. 相似文献
5.
Improved surface-based geophysical technologies that are commercially available provide a new level of detail that can be used to guide ground water remediation. Surface-based multielectrode resistivity methods and tomographic seismic refraction techniques were used to image to a depth of approximately 30 m below the surface at the Natural and Accelerated Bioremediation Research Field Research Center. The U.S. Department of Energy (DOE) established the research center on the DOE Oak Ridge Reservation in Oak Ridge, Tennessee, to conduct in situ field-scale studies on bioremediation of metals and radionuclides. Bioremediation studies are being conducted on the saprolite, shale bedrock, and ground water at the site that have been contaminated with nitrate, uranium, technetium, tetrachloroethylene, and other contaminants (U.S. DOE 1997). Geophysical methods were effective in imaging the high-ionic strength plume and in defining the transition zone between saprolite and bedrock zones that appears to have a significant influence on contaminant transport. The geophysical data were used to help select the location and depth of investigation for field research plots. Drilling, borehole geophysics, and ground water sampling were used to verify the surface geophysical studies. 相似文献
6.
Understanding the mechanisms of bacterial transport in aquifers is important in developing bioremediation strategies. Collision efficiency (alpha) is one important parameter used in modeling bacterial transport. This study was undertaken to measure change in alpha with distance by performing a bacterial transport experiment in Oyster, Virginia. Following injection of a bacterium, Comamonas sp., into a well, water samples were collected at various distances along the flowpath and injected into columns packed with homogenized South Oyster focus area sediment. Zeta potentials of the bacteria in the samples were measured. Values of alpha were determined at various locations in the field in two ways: based on field breakthrough concentrations at the sampling points and based on column breakthrough concentrations. The alpha values estimated from field breakthrough decreased with distance, whereas those estimated from column breakthrough increased with distance. Bacterial cell surface charge became progressively more negative with distance in the field. We hypothesize that the apparent contradiction between field and column alpha values was caused by differences in the flow of the two systems. Flow in the columns was forced to occur through fine-grained zones of iron and aluminum hydroxide coatings that selectively removed the most negatively charged bacteria. In contrast, in the field, the injected cells did not come into contact with the positively charged coatings because the bulk solution bypassed them due to heterogeneous hydraulic properties. These results suggest that laboratory-based models may underestimate bacterial transport distance in the field. A more realistic approach may be necessary to capture the degree of heterogeneity. 相似文献
7.
《Advances in water resources》2007,30(6-7):1680-1695
Bacterial adhesion and motility are studied at the pore scale by focusing on two interrelated aspects of transport: wall attachment/detachment (reversible sorption) and the role of convection and pore geometry on adhesion. Motility is also examined through use of Brownian dynamics. Bacteria motility and reversible attachment/detachment are incorporated with a numerical laminar flow solver. Since individual bacteria are modeled, the results apply to low concentrations/coverage. Pore geometries consistent with a microflow cell of variable cross sectional area are used. This exploratory modeling work precedes an ongoing microflow cell experimental study and more detailed Lévy particle models. Adsorption reactions occurring over different time scales are modeled as multimodal distributions with power law tails. Computations show the relative magnitude of bacterial motility to advection controls the average number of collisions against solid walls. Variable cross section in pore geometry changes hydrodynamic conditions for deposition (e.g., variable shear stress). In regions of reduced cross sectional area, the ratio of bacteria motility to average velocity is smaller and results in less collisions and reduced retardation. Additionally, reduced cross sectional area increases both wall shear stress and vorticity which should be considered in adhesive models. While the shear forces acting on a particle deposited at the wall work on a spatial scale of the microbe’s size, adhesive forces may be confined to tens of nanometers. Multimodal adhesion causes the first passage time distributions to have long tails. 相似文献
8.
Remediation of ground water and soil contamination at the Wells G & H Superfund Site, Woburn, Massachusetts, uses technologies that reflect differences in hydrogeologic settings, concentrations of volatile organic compounds (VOCs), and costs of treatment. The poorly permeable glacial materials that overlie fractured bedrock at the W.R. Grace property necessitate use of closely spaced recovery wells. Contaminated ground water is treated with hydrogen peroxide and ultraviolet (UV) oxidation. At UniFirst, a deep well completed in fractured bedrock removes contaminated ground water, which is treated by hydrogen peroxide, UV oxidation, and granular activated carbon (GAC). The remediation system at Wildwood integrates air sparging, soil-vapor extraction, and ground water pumping. Air stripping and GAC are used to treat contaminated water; GAC is used to treat contaminated air. New England Plastics (NEP) uses air sparging and soil-vapor extraction to remove VOCs from the unsaturated zone and shallow ground water. Contaminated air and water are treated using separate GAC systems. After nine years of operation at W.R. Grace and UniFirst, 30 and 786 kg, respectively, of VOCs have been removed. In three years of operation, 866 kg of VOCs have been removed at Wildwood. In 15 months of operation, 36 kg of VOCs were removed at NEP. Characterization work continues at the Olympia Nominee Trust, Whitney Barrel, Murphy Waste Oil, and Aberjona Auto Parts properties. Risk assessments are being finalized that address heavy metals in the floodplain sediments along the Aberjona River that are mobilized from the Industri-Plex Superfund Site located a few miles upstream. 相似文献
9.
Matrix diffusion can attenuate the rate of plume migration in fractured bedrock relative to the rate of ground water flow for both conservative and nonconservative solutes of interest. In a system of parallel, equally spaced constant aperture fractures subject to steady-state ground water flow and an infinite source width, the degree of plume attenuation increases with time and travel distance, eventually reaching an asymptotic level. The asymptotic degree of plume attenuation in the absence of degradation can be predicted by a plume attenuation factor, beta, which is readily estimated as R' (phi(m)/phi(f)), where R' is the retardation factor in the matrix, phi(m) is the matrix porosity, and phi(f) is the fracture porosity. This dual-porosity relationship can also be thought of as the ratio of primary to secondary porosity. Beta represents the rate of ground water flow in fractures relative to the rate of plume advance. For the conditions examined in this study, beta increases with greater matrix porosity, greater matrix fraction organic carbon, larger fracture spacing, and smaller fracture aperture. These concepts are illustrated using a case study where dense nonaqueous phase liquid in fractured sandstone produced a dissolved-phase trichloroethylene (TCE) plume approximately 300 m in length. Transport parameters such as matrix porosity, fracture porosity, hydraulic gradient, and the matrix retardation factor were characterized at the site through field investigations. In the fractured sandstone bedrock examined in this study, the asymptotic plume attenuation factors (beta values) for conservative and nonconservative solutes (i.e., chloride and TCE) were predicted to be approximately 800 and 12,210, respectively. Quantitative analyses demonstrate that a porous media (single-porosity) solute transport model is not appropriate for simulating contaminant transport in fractured sandstone where matrix diffusion occurs. Rather, simulations need to be conducted with either a discrete fracture model that explicitly incorporates matrix diffusion, or a dual-continuum model that accounts for mass transfer between mobile and immobile zones. Simulations also demonstrate that back diffusion from the matrix to fractures will likely be the time-limiting factor in reaching ground water cleanup goals in some fractured bedrock environments. 相似文献
10.
Chuan Zhao Thorsten Brinkhoff Malte Burchardt Meinhard Simon Gunther Wittstock 《Ocean Dynamics》2009,59(2):305-315
Adhesion of two marine bacteria Shewanella sp. strain T1 and Pseudoalteromonas sp. strain T8, on differently terminated alkanethiolate self-assembled monolayers on gold was investigated. The selected
model surfaces—terminated by CH3, OH, NH2, COOH, OH-terminated oligo(ethylene glycol), and methyl-terminated oligo(ethylene glycol)—are characterized by contact angle
measurement using water, methylene iodide, 1-bromonaphthalene, and formamide. Surface free energies were calculated. Cell
counting of the two bacterial strains on the model surfaces after different times revealed differences between the two strains
by at least one order of magnitude. For the different surfaces, the bacteria showed comparably small selectivity. Atomic force
microscopy images of adhered bacteria showed very different fingerprints on the different surfaces.
Electronic supplementary material Supplementary material is available in the online version of this article at and is accessible to authorized users. 相似文献
11.
根据天津波地表加速度记录和相应实测场地钻孔资料,采用等效线性化方法模拟土体非线性,进行了深厚软土场地基岩地震动反演,研究了频率截断对深厚软土场地基岩地震动反演的影响,为天津波地表加速度记录提供了相应的基岩地震动输入。研究表明:不同截断频率情况下,反演得到的基岩地震动峰值和反应谱曲线形状均相差悬殊,但反演后再正演所得到的地面运动与原地表记录相比,加速度时程曲线形状和峰值大小、反应谱曲线形状和峰值大小均非常接近;可根据工程需要对地表加速度记录进行不同频率的截断,反演得到所需强度的基岩地震动。 相似文献
12.
Owing to increased demands on ground water accompanied by increased drawdowns, technologies that use recharge options, such as aquifer storage recovery (ASR), are being used to optimize available water resources and reduce adverse effects of pumping. In this paper, three representative ground water flow models were created to assess the impact of hydrogeologic and operational parameters/factors on recovery efficiency of ASR systems. Flow/particle tracking and solute transport models were used to track the movement of water during injection, storage, and recovery. Results from particle tracking models consistently produced higher recovery efficiency than the solute transport models for the parameters/properties examined because the particle tracking models neglected mixing of the injected and ambient water. Mixing between injected and ambient water affected recovery efficiency. Results from this study demonstrate the interactions between hydrogeologic and operational parameters on predictions of recovery efficiency. These interactions are best simulated using coupled numerical ground water flow and transport models that include the effects of mixing of injected water and ambient ground water. 相似文献
13.
Analysis of the vapor in passive vapor samplers retrieved from a streambed in fractured rock terrain implied that volatile organic carbon (VOC) discharge from ground water to surface water substantially increased following installation of a contaminant recovery well using air rotary drilling. The air rotary technique forced air into the aquifer near the stream. The injection produced an upward hydraulic gradient that appears to have transported water and contaminants from deeper parts of the aquifer through fractures into shallow parts of the aquifer. Once in the shallow flow regime, the contamination was transported to the stream, where it discharged during the next several weeks following well installation. After the recovery well was activated and began continuously pumping contaminated ground water to a treatment facility, the VOC concentrations in the stream bottom passive vapor samplers decreased to below detectable concentrations, suggesting that the withdrawal had captured the contaminated ground water that previously had discharged to the stream. 相似文献
14.
Natural bedrock rivers flow in self‐formed channels and form diverse erosional morphologies. The parameters that collectively define channel morphology (e.g. width, slope, bed roughness, bedrock exposure, sediment size distribution) all influence river incision rates and dynamically adjust in poorly understood ways to imposed fluid and sediment fluxes. To explore the mechanics of river incision, we conducted laboratory experiments in which the complexities of natural bedrock channels were reduced to a homogenous brittle substrate (sand and cement), a single sediment size primarily transported as bedload, a single erosion mechanism (abrasion) and sediment‐starved transport conditions. We find that patterns of erosion both create and are sensitive functions of the evolving bed topography because of feedbacks between the turbulent flow field, sediment transport and bottom roughness. Abrasion only occurs where sediment impacts the bed, and so positive feedback occurs between the sediment preferentially drawn to topographic lows by gravity and the further erosion of these lows. However, the spatial focusing of erosion results in tortuous flow paths and erosional forms (inner channels, scoops, potholes), which dissipate flow energy. This energy dissipation is a negative feedback that reduces sediment transport capacity, inhibiting further incision and ultimately leading to channel morphologies adjusted to just transport the imposed sediment load. Copyright © 2007 John Wiley & Sons, Ltd. 相似文献
15.
Ivan K. Gall Robert W. Ritzi Jr. A. Dwight Baldwin Jr. Paul D. Pushkar Cindy K. Carney Joseph F. Talnagi Jr. 《Ground water》1995,33(2):197-206
Two hypotheses have previously been proposed for the source of elevated radon in ground water of southwestern Ohio: (1) penecontemporaneous uranium at the Silurian-Ordovician unconformity, and/or (2) parent radionuclides transported from fragments of uranium-rich Ohio Shale within the glacial drift above the aquifer. To further test the first hypothesis, vertical profiles of dissolved radon in ground water and uranium in rock cores were obtained at two locations immediately underlain by the Silurian/Ordovician unconformity. Radon concentrations exceeding 1000 pCi/l occurred in zones where the bedrock had uranium concentrations greater than 1.5 ppm. Radon concentrations of less than 500 pCi/l occurred in zones where the rock had uranium concentrations below 0.25 ppm. A log-linear regression model between uranium and radon had a correlation coefficient of 0.82. Three aspects of the results support the hypothesis that the source is transported, although not necessarily from fragments of Ohio Shale. First, the high uranium-radon zones did not occur consistently or exclusively at the Silurian/Ordovician unconformity. Second, the high uranium-radon zones are correlated to fracture zones having a higher hydraulic conductivity and thus appear to be related to the zones of greater flow and transport. Third, the amount of uranium-radon disequilibrium increases exponentially with increasing hydraulic conductivity. The hypothesis of a penecontemporaneous source, not supported by our study, arose when previous investigators conducted regional surveys of domestic wells and springs and found a correspondence between elevated radon and the location of the Silurian-Ordovician unconformity. The observations of the previous investigators can be explained by the fact that the basal Silurian is in some places a horizon of higher hydraulic conductivity that facilitates transport. The two most probable external sources of uranium would be uranium-containing detritus in the glacial drift or uranium-containing phosphate fertilizers spread on the surface. Given that the uranium was transported into the aquifer during the Holocene, it could not have generated enough radium in the time elapsed since entering the aquifer to produce the radon levels that were measured. This observation indicates that radium was cotransported with uranium into the zones of high radon. 相似文献
16.
《Ground Water Monitoring & Remediation》1985,5(1):51-57
The possible mine will remove a gently, less than 50 feet per mile, westerly dipping Springfield coal from an area covered by glacial till and some channel sands and gravel. The area is flat, with less than 20 feet of relief in a square mile. The channel sands and gravels, the till and the bedrock are capable of yielding ground water at 5 to 75,3 to 10, and 1 to 10 gallons per minute (gpm), respectively. The ground water in the drift and the shallow bedrock is calcium-bicarbonate type, contrasting with the sodium-bicarbonate type in the deep bedrock. The surface mine will feature selective handling of overburden. The probable hydrologic consequences of the mine will be 1) a short-term, areally limited dewatering, 2) an increase in dissolved solids, 3) a change in ground water chemistry in some areas to a calcium-bicarbonate sulfate water, 4) an increase in ground water storage, and 5) a new integrated surface water system. The proposed ground water monitoring system will include seven monitoring wells in the glacial material and one in the bedrock. The primary effort in ground water monitoring to the west of the mine will be to detect changes in the quality of the ground water, whereas to the east, changes in both quality and quantity will need to be monitored intensively. 相似文献
17.
Sam Roberson 《地球表面变化过程与地形》2008,33(13):1931-1947
This paper considers the links between structure, sediment transport and sediment delivery at Glacier de St. Sorlin, France. Sediment transported by the glacier is concentrated at flow‐unit boundaries as medial moraines, controlled by the position of bedrock outcrops in the accumulation area. Rockfall entrained within primary stratification is tightly folded at flow‐unit boundaries under high cumulative strains and laterally compressive stress. High cumulative strains and laterally compressive stresses lead to the development of longitudinal foliation from primary stratification. Folding elevates subglacial sediments into foliation‐parallel debris ridges, which are exposed in the ablation area. Crevasses and shear planes within the glacier have little control on sediment transport. Debris stripes in the proglacial area are morphologically similar to foliation‐parallel debris ridges; however, they are not structurally controlled, but formed by fluvial erosion. The conclusion of this study is that at Glacier de St. Sorlin proglacial sediment‐landform associations are subjected to intense syn‐ and post‐depositional modification by high melt‐water discharges, hence their composition does not reflect that of sediments melting out at the terminus. The action of melt water limits the potential of the sedimentary record to be used to constrain numerical models of past glacier dynamics in debris‐poor glacierized Alpine catchments. Copyright © 2008 John Wiley & Sons, Ltd. 相似文献
18.
In a recent field study of ground water/surface water interaction between a bedrock stream and an underlying fractured rock aquifer, it was determined that the majority of ground water discharge occurred through sparsely located vertical fractures. In this paper, the dominant mechanisms governing ground water/surface water exchange in such an environment are investigated using a numerical model. The study was conducted using several conceptual models based on the field study results. Although the field results provided the motivation for the modeling study, it was not intended to match modeling and field results directly. In addition, the extent of capture zones for discharging or recharging fractures was explored. The results of this study are intended to provide a better understanding of contaminant migration in the vicinity of bedrock streams. Based on the numerical results, the rate of ground water discharge (or recharge) was found to depend on the aperture size of the discharging feature, and on the distribution of hydraulic head with depth within the fracture network. It was determined that the extent of both the capture zone and reverse capture zone for an individual fracture can be extremely large, and will be determined by the height of the stream stage, the fracture apertures of the network, and the hydraulic-head distribution within the network. Because both the stream stage and the hydraulic-head distribution are transient, the size of the capture zone and/or the reverse capture zone for an individual fracture may change significantly over time. As a result, the migration path for contaminants within the fracture network and between the surface and subsurface will also vary significantly with time. 相似文献
19.
随着强震台网的密布及观测记录的增加,为研究各类局部场地地震反应预测模型的合理性提供了有效的参考依据,也使利用强震记录及场地条件研究地震动特征成为可能。选取场地地质参数资料和地震记录数据齐全的日本小田原（Ashigara Valley）盲测试验场地,通过对比不同地震动输入方式及场地反应分析模型,研究地震动特征,分析现有模型的优劣。基于1990年8月5日M5.1强震事件的地表基岩记录和地下基岩地震记录,采用地下台强震记录直接输入、地表基岩台强震记录减半为基底地震动输入、地表基岩台强震记录反演为基底地震动输入作为3种基岩地震动输入。基于局部场地条件分别建立一维等效线性模型、二维黏弹性模型及二维时域等效线性化模型等工程中常用的场地数值分析模型,进行局部场地地震反应分析,预测该盲测场地的地表地震动特征,并与对应的实测强震记录结果进行对比,分析不同基岩地震动输入方式对预测地震动特征及地表土层反应谱特征的影响,重点分析地震动输入、土体非线性、场地横向不均匀性及几何与非线性特征共同作用等因素对地表地震动特征的影响,以期为地表地震动的合理预测提供参考。 相似文献