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1.
Antarctic marine bacteria were screened for their ability to degrade polychlorinated biphenyls (PCB) as the sole carbon and energy source at both 4 degrees C and 15 degrees C. PCB-degrading isolates (7.1%) were identified by sequencing their 16S rDNA as Pseudoalteromonas, Psychrobacter and Arthrobacter members. One representative isolate per genera was selected for evaluating the biodegradative potential under laboratory scale and phenotypically characterized. Removal of individual PCB congeners was between 35.6% and 79.8% at 4 degrees C and between 0.4% and 82.8% at 15 degrees C. Differences in the removal patterns of PCB congeners were observed in relation to the phylogenetic affiliation: Arthrobacter isolate showed similar biodegradation efficiencies when growing at 4 degrees C and 15 degrees C, while Pseudoalteromonas better degraded PCBs at 15 degrees C. No biodegradation was detected for Psychrobacter isolate at 4 degrees C. Results obtained highlight the occurrence of PCB-degrading bacteria in Antarctic seawater and suggest the potential exploitation of autochthonous bacteria for PCB bioremediation in cold marine environments.  相似文献   

2.
The Ischia hydrothermal system was analysed through hydrogeological and microbial community investigations. Mesophilic communities were detected in two cold springs, suggesting a negligible influence of thermal circuits in freshwater sub‐systems which are mainly or only fed by local precipitations. Thermophilic and extremely thermophilic bacteria were detected in two wells, according to higher water temperatures (61 and 85 °C), even if the two communities show significant differences. In one well, thermophilic and extremely thermophilic bacteria are associated with strains belonging to ε‐Proteobacteria isolated in different sulphur‐rich carbonate environments. This association suggests a greater influence on ascending hot fluids that interact with the carbonate basement of volcanic rocks. In the other well, thermophilic and extremely thermophilic bacteria are associated with strains isolated in cold hypersaline environments or in aquatic habitats where terrestrial and marine components are coupled. This association supports the fact that seawater intrusion can affect this part of Ischia, according to results of hydrogeological and geochemical surveys. Differences in groundwater temperature and bacterial communities are probably mainly due to differences in permeability between volcanic rocks and differences in hydrogeologic behaviour between faults in the upper carbonate basement, above the deep magma chamber, that influence relationships between ascending hot fluids and local recharge. This study contributes to discussion of the reliability of the actual behaviour models of the Ischia system, based on the results of geochemical and isotopic investigations, and, in a wider context, it shows that microbial community investigations may be a valuable supplementary tool for analysing hydrothermal system behaviour. Copyright © 2010 John Wiley & Sons, Ltd.  相似文献   

3.
Most-probable-number (MPN) dilution series were used to enumerate and isolate bacteria from bulk water, suspended aggregates, the oxic layer, and the oxic–anoxic transition zone of the sediment of a tidal flat ecosystem in the southern North Sea. The heterotrophic aerobic bacteria were able to grow on agar-agar, alginate, cellulose, chitin, dried and ground Fucus vesiculosus, Marine Broth 2216, palmitate, and starch. MPN counts of bulk water and aggregate samples ranged between 0.18?×?101 and 1.1?×?106 cells per milliliter and those of the sediment surface and the transition zone between 0.8?×?101 and 5.1?×?107 cells per gram dry weight. Marine Broth and F. vesiculosus yielded the highest values of all substrates tested and corresponded to 2.3–32% of 4,6-diamidinophenyl indole cell counts. Strains of seven phylogenetic classes were obtained: Actinobacteria, Bacilli, α- and γ-Proteobacteria, Sphingobacteria, Flavobacteria, and Planctomycetacia. Only with agar-agar as substrate could organisms of all seven classes be isolated.  相似文献   

4.
This study combined bacterial incubation and hydrogeochemical methods to investigate denitrification in the surface water, top soil (0–20 cm), and shallow groundwater of the Ochi wetland in Japan. Surface water was rich in nitrate (40 mg/l) and denitrifying bacteria (700 per ml). Three functional zones in the wetland were identified in the top soil and shallow groundwater. In the upstream portion of the wetland (Zone I), the counts of denitrifying bacteria in top soil increased from 5200 to 14 970 per ml and nitrate decreased from 25·4 to 1·8 mg/l. Organic carbon concentrations decreased as sulfate increased from 4·0 to 9·6 mg/l in this zone. In the middle‐stream of the wetland (Zone II), all concentrations of major anions, iron, organic carbon, and total nitrogen content in top soil were relatively constant, but the counts of denitrifying bacteria increased up to 70 200 per ml. In the downstream portion of the wetland (Zone III), complete removal of nitrate resulted in sharp reduction of counts of denitrifying bacteria. Correspondingly, dissolved oxygen (DO), organic carbon, and total nitrogen increased in this zone. Counts of denitrifying bacteria were lower in shallow groundwater than in top soil; nitrate concentrations in shallow groundwater were also very low in this zone. DO and the oxidation/reduction potential data suggest that groundwater flows to the surface along an extended flow path, thus providing nitrate for the denitrifying community. Copyright © 2011 John Wiley & Sons, Ltd.  相似文献   

5.
Vertical distribution of fossil carotenoids in a sediment core from meromictic Mahoney Lake was studied. Besides okenone and demethylated okenone, lutein and zeaxanthin and-carotene isomers were identified. No carotenoids typical for purple nonsulfur or green sulfur bacteria were detected. The ratio of zeaxanthin to lutein (above 1:1 in all samples) indicates a dominance of cyanobacteria over green algae in the phytoplankton assemblages of the past. Okenone, which is found exclusively in Chromatiaceae, was the dominating carotenoid in all sediment zones.The oldest sediment layers containing okenone were deposited 11 000 years ago. Between 9000 and 7000 and since 3000 years b.p., Chromatiaceae reached a considerable biomass in the lake. Vertical changes in okenone concentration were not related to changes of paleotemperatures. In contrast, okenone concentrations decreased during periods of volcanic ash input. During most of the lake history, however, mean okenone concentrations were positively correlated with sedimentation rates. This indicates that vertical changes of okenone concentration in the sediment reflect past changes of purple sulfur bacterial biomass in the lake.According to these results, the past limnology of Mahoney Lake resembled that of the present with a sulfide-containing monimolimnion and a well-developed population of okenone-bearing purple sulfur bacteria.  相似文献   

6.
对武汉东湖水柱和沉积物中几丁质分解菌的种类组成、数量分布及动态的研究结果表明:几丁质分解菌中.放线菌居于优势,在水柱中约为总数的50—72.3％;在沉积物中约为63—88.5％。经鉴定的102株放线菌中,链霉菌属(Steptomyces)占总数的70.6％;链孢囊菌属(Streptosporangium)、小单孢菌属(Micromonospora)、小多孢菌属(Micropolyspora)分别为8.8％、9.8％和10.8％。细菌中主要是芽孢杆菌属(Bacillus),占鉴定菌株数的42％,其次为沙雷氏菌属(Serratia)、气单胞菌属(Aeromonas)、微球菌属(Micrococcus)和短杆菌属(Brevibacterium)的一些菌株。在数量分布上,沉积物中高于水柱2—3个数量级;东湖Ⅰ站高于Ⅱ站。随着季节更迭,水温上升,其数量亦有相应变化。对菌株分解几丁质的能力和其它生物活性作了比较,并就所得结果进行了讨论。  相似文献   

7.
Adhesion of two marine bacteria Shewanella sp. strain T1 and Pseudoalteromonas sp. strain T8, on differently terminated alkanethiolate self-assembled monolayers on gold was investigated. The selected model surfaces—terminated by CH3, OH, NH2, COOH, OH-terminated oligo(ethylene glycol), and methyl-terminated oligo(ethylene glycol)—are characterized by contact angle measurement using water, methylene iodide, 1-bromonaphthalene, and formamide. Surface free energies were calculated. Cell counting of the two bacterial strains on the model surfaces after different times revealed differences between the two strains by at least one order of magnitude. For the different surfaces, the bacteria showed comparably small selectivity. Atomic force microscopy images of adhered bacteria showed very different fingerprints on the different surfaces. Electronic supplementary material  Supplementary material is available in the online version of this article at and is accessible to authorized users.  相似文献   

8.
Methane fermentation is widely used to dispose of sewage sludge at wastewater treatment plants (WWTPs), due to production of renewable energy in the form of biogas. Antibiotics present in wastewater may accumulate in a sewage sludge. The aim of the present study is to investigate the impact of three antibiotics from different classes in three different doses on methane production from sewage sludge. For this purpose, metronidazole (MET), amoxicillin (AMO), and ciprofloxacin (CIP) are individually added to anaerobic reactors with sewage sludge collected from municipal WWTP. The antibiotics’ highest concentration (1024 mg kg?1 of AMO; 512 mg kg?1 of MET and CIP) lowers methane production and methane content in biogas. MET exerts the most marked effect and lowers methane production to 36.8 ± 3.7 mL CH4 kg?1 volatile solids. Tested antibiotics probably inhibit methanogenic archaea, which results in volatile fatty acids (VFAs) accumulation. Addition of MET results in accumulation of many kinds of VFAs with the highest concentration of acetic acid (17.52 ± 1.85 g L?1). The addition of of AMO results in accumulation of butyric acid only (253.00 ± 15.89 g L?1). However, addition of CIP results in accumulation of mainly acetic acid (7.58 ± 0.82 g L?1) and isovaleric acid (2.01 ± 0.41 g L?1). Next, synergistic effect of these antibiotics in a low concentration of 16 mg kg?1 of AMO, 8 mg kg?1 of MET, and 2 mg kg?1 of CIP is measured in semi‐continuous conditions and causes inbibition of methane production and accumulation of VFAs.  相似文献   

9.
Both laboratory experiments and numerical modelling were conducted to study the biodegradation and transport of benzene–toluene–xylenes (BTX) in a simulated semi‐confined aquifer. The factors incorporated into the numerical model include advection, hydrodynamic dispersion, adsorption, and biodegradation. The various physico‐chemical parameters required by the numerical model were measured experimentally. In the experimental portion of the study, BTX compounds were introduced into the aquifer sand. After the contaminants had been transported through the system, BTX concentrations were measured at 12 equally spaced wells. Subsequently, microorganisms obtained from the activated sludge of a sewage treatment plant and cultured in BTX mixtures were introduced into the aquifer through the 12 sampling wells. The distribution data for BTX adsorption by the aquifer sand form a nonlinear isotherm. The degree of adsorption by the sand varies, depending on the composition of the solute. The degradation time, measured from the time since the bacteria were added to the aquifer until a specific contaminant was no longer detectable, was 35–42 h for BTX. The dissolved oxygen, after degradation by BTX compounds and bacteria, was consumed by about 40–60% in the entire simulated aquifer; thus the aerobic conditions were maintained. This study provides insights for the biodegradation and transport of BTX in aquifers by numerical modelling and laboratory experiments. Experimental and numerical comparisons indicate that the results by Monod degradation kinetics are more accurate than those by the first‐order degradation kinetics. Copyright © 2002 John Wiley & Sons, Ltd.  相似文献   

10.
Norman E. Peters 《水文研究》2009,23(20):2860-2878
A long‐term stream water quality monitoring network was established in the city of Atlanta, Georgia during 2003 to assess baseline water quality conditions and the effects of urbanization on stream water quality. Routine hydrologically based manual stream sampling, including several concurrent manual point and equal width increment sampling, was conducted ～12 times annually at 21 stations, with drainage areas ranging from 3·7 to 232 km2. Eleven of the stations are real‐time (RT) stations having continuous measures of stream stage/discharge, pH, dissolved oxygen, specific conductance, water temperature and turbidity, and automatic samplers for stormwater collection. Samples were analyzed for field parameters, and a broad suite of water quality and sediment‐related constituents. Field parameters and concentrations of major ions, metals, nutrient species and coliform bacteria among stations were evaluated and with respect to watershed characteristics and plausible sources from 2003 through September 2007. Most constituent concentrations are much higher than nearby reference streams. Concentrations are statistically different among stations for several constituents, despite high variability both within and among stations. Routine manual sampling, automatic sampling during stormflows and RT water quality monitoring provided sufficient information about urban stream water quality variability to evaluate causes of water quality differences among streams. Fecal coliform bacteria concentrations of most samples exceeded Georgia's water quality standard for any water‐usage class. High chloride concentrations occur at three stations and are hypothesized to be associated with discharges of chlorinated combined sewer overflows, drainage of swimming pool(s) and dissolution and transport during rainstorms of CaCl2, a deicing salt applied to roads during winter storms. One stream was affected by dissolution and transport of ammonium alum [NH4Al(SO4)2] from an alum‐manufacturing plant; streamwater has low pH (<5), low alkalinity and high metals concentrations. Several trace metals exceed acute and chronic water quality standards and high concentrations are attributed to washoff from impervious surfaces. Published in 2009 by John Wiley & Sons, Ltd.  相似文献   

11.
Banded iron formations are the most characteristic of Archean–Paleoproterozoic sediment records. Laminated textures resembling banded iron formations can be observed in modern hot‐spring environments. Using sedimentological and microbiological techniques, we investigated the processes of laminar formation and considered the origin of lamination textures. An iron‐rich deposit at the Okuoku‐hachikurou hot spring in Japan exhibits sub‐millimeter laminations consisting of bacteria‐induced ferrihydrite and aragonite. The ferrihydrite particles are spherical and exhibit fine lamination, up to 100 µm thick in ferrihydrite‐rich parts. In aragonite‐rich parts, ferrihydrite particles form filamentous textures with diameters of 10–30 µm, but not laminations. Textural analysis using scanning electron microscopy and phylotype analysis using 16S rRNA indicated the bacterial contribution to ferrihydrite precipitation. A sheath‐like fabric showing a meshwork of nanometer‐order organic filaments, and sheath‐forming bacteria were observed in the deposit specimen etched by citric acid. Phylotype analysis detected in the iron‐rich deposits some bacterial types related to cyanobacteria, purple bacteria, and iron‐oxidizing bacteria. Iron‐oxidizing bacteria probably were responsible for precipitation of the ferrihydrite. Chemolithoautotrophic iron‐oxidizing bacteria are microaerophilic and thrive on Fe(II) in a redox gradient, but dissolved oxygen was not detected in the Okuoku‐hachikurou hot spring. Thus, a certain supply of oxygen is needed for metabolism of the microaerophilic iron‐oxidizing bacteria. The distribution of photosynthetic pigments in the iron‐rich parts indicates that the most likely source of oxygen is photosynthesis by cyanobacteria. This symbiotic relationship between cyanobacteria and iron‐oxidizing bacteria can explain the laminated texture of iron‐rich deposits in the Okuoku‐hachikurou hot spring. These laminations may reflect changes in photosynthetic intensity. There is presently some debate about the bacterial groups that may have played roles in precipitation of banded iron formations. This study presents a new bacterial model for iron precipitation and may provide a mechanism for sub‐millimeter laminations in banded iron formations deposited in shallow water.  相似文献   

12.
Magnetofossils in the sediment of Lake Baikal, Siberia   总被引：1，自引：0，他引：1  
A multidisciplinary approach involving rock-magnetics, transmission electron microscopy, and X-ray diffraction was used to identify a biogenic magnetite component in the Lake Baikal, Siberia, sedimentary magnetic record. The distinctive biogenic component to the magnetic record occurs as chains of single-domain, elongate hexagonal and tear-drop cone-shaped magnetite particles. These magnetofossils are inferred to be magnetosomes produced by magnetotactic bacteria living in the surficial sediment throughout Lake Baikal. Postdepositional reduction diagenesis results in the loss of the fine-grained magnetofossils at depth. In addition, this study shows that the fine-grained magnetofossils are removed by the process of storage diagenesis during long periods (21 months) of core storage, which results in a change to a coarser grained, slightly higher coercivity bulk magnetic mineral assemblage. Although the Lake Baikal sedimentary magnetic record has several distinct and complex components, by determining their origin this study has shown that the sediments are well suited for environmental magnetic study.  相似文献   

13.
An experimental study on reduction of U (Ⅵ) by anaerobic bacteria, Shewane//a putrefaciens, is first reported here in China. The experimental conditions were: 35℃ and pH =7.0-7.4, corresponding to a physicochemical environments in which the sandstone-hosted interlayer oxidation-zone type uranium deposit formed in Northwest China's Xinjiang. Bacteria adopted in the present experiment, Shewanella putrefaciens, occur extensively in natural environment. Our study shows that nano-crystal precipitates of uraninite quickly occurred on the surface of the cells within one week. It was found that the pitchblende was characterized by a random arrangement of uraninite nanocrystals (2-4 nm) in it, significantly different from natural pitchblende in which uraninite nanocrystals are arranged in order. Finally, a possible mechanism of uranium biomineralization by microorganisms in the deposits is discussed. Our investigation may supply a technical train of thoughts for bioremediation of nuclear-contaminated water environments and for underground dissolving extraction of the sandstone-hosted uranium ores.  相似文献   

14.
采用多管发酵培养法检测水样中致腐蚀并富集出测试菌群,设置杀菌剂浓度梯度,筛选出有效的杀菌剂,对不同配比及含不同杀菌剂的树脂涂层进行耐腐蚀及杀菌效果检测,并对其中效果最好的２２号杀菌菌剂涂层进行了长效性实验和耐菌性实验,结果表明含２２号杀菌剂的抗菌涂料是一种很好的抗水库中微生物腐蚀的材料。  相似文献   

15.
对贵州草海沉积物柱芯碳酸盐碳同位素组成测定的结果显示,其δ13C值的变化范围为- 14.25‰至23.10‰,变化幅度为37.35％,这是迄今所发现的湖泊沉积物碳酸盐中最大的碳同位素变化幅度,其中的部分同位素值也是迄今发现的最大碳同位素值.综合碳酸盐氧同位素、碳酸盐含量和有机质含量指标,认为草海沉积物碳酸盐碳同位素组成...  相似文献   

16.
A lot of fossil bacteria and algae, which were re-placed by uraninite ( pitchblende), were first found inthe ores when the authors studied the sandstone-hostedinterlayer oxidation-zone type uranium deposits innorthwest China[1,2]. It is known that a powerful radia-tion from the uranium ores is harmful and, evendeadly to any life, including microorganism. Whatmechanism does this inconceivable fact result from?Up to the present time in China, there has been no ex-perimental investigation on red…  相似文献   

17.
The deltaic estuarine system of the Matang Mangrove Forest Reserve of Malaysia is a site where several human settlements and brackish water aquaculture have been established. Here, we evaluated the level of fecal indicator bacteria (FIB) and the presence of potentially pathogenic bacteria in the surface water and sediments. Higher levels of FIB were detected at downstream sampling sites from the fishing village, indicating it as a possible source of anthropogenic pollution to the estuary. Enterococci levels in the estuarine sediments were higher than in the surface water, while total coliforms and E. coli in the estuarine sediments were not detected in all samples. Also, various types of potentially pathogenic bacteria, including Klebsiella pneumoniae, Serratia marcescens and Enterobacter cloacae were isolated. The results indicate that the Matang estuarine system is contaminated with various types of potential human bacterial pathogens which might pose a health risk to the public.  相似文献   

18.
Bathing water quality is an important public health issue, mainly because of fecal contamination. In 2006, the European Commission (EC) adopted a new directive with respect to recreational bathing waters that calls for stricter standards and reduces the number of laboratory tests done in routine beach monitoring from nineteen to two bacterial indicators, namely, Escherichia coli and intestinal enterococci, replacing policies of the EC Bathing Water Directive that have existed since 1976. Our practice in Portugal is in line with this international development, and this study demonstrates the equivalency of the new bacteriological parameters with the old. The water quality of 25 coastal beaches was surveyed using both new and old microbiological indicators of fecal contamination. Statistical analysis demonstrated equivalency of the results obtained for fecal coliforms with Escherichia coli and for fecal streptococci with intestinal enterococci.  相似文献   

19.
固定化土著氮循环细菌在城市湖泊水体净化中的应用   总被引：2，自引：1，他引：1  
从镇江金山湖天然水体中筛选分离出土著氨化、亚硝化、硝化和反硝化细菌,对氮循环细菌固定化后在镇江金山湖示范工程区进行水体净化应用.结果表明,运行一段时间后水质得到明显改善,氨氮、亚硝酸盐氮和硝酸盐氮以及总氮浓度处于动态变化中,有降低的趋势;氨氮含量已经达到Ⅱ类指标,总氮和COD分别达到Ⅴ类、Ⅲ类指标;分析结果还显示硝酸盐氮是金山湖水体中总氮的主要存在形式,与水体荒漠化状态有关;固定化氮循环细菌通过扩散,可以增加水体及植物根区的微生物数量,高出对比组1-3个数量级;固定化氮循环细菌技术对荒漠化水体氨氮、总氮和COD去除具有重要意义.  相似文献   

20.
Research to understand and remediate coastal pollution is moving toward a multitiered approach in which traditional enumeration of fecal indicators is accompanied by molecular analysis of a variety of targets. Technology that rapidly detects multiple microbial contaminants would benefit from such an approach. The Luminex 100 system is a suspension array that assays multiple analytes rapidly in a single well of a microtiter plate. The ability of the system to simultaneously detect multiple fecal indicating bacteria in environmental samples was tested. Primer/probe sets were designed to simultaneously detect the following fecal indicators: the Bacteroides fragilis group, Enterococcus spp., Escherichia coli and Shigella spp., Bacteroides distasonis, and Ent. faecalis. Specificity and sensitivity of the Luminex probes was tested against laboratory cultures. In addition, sequencing, culture plate testing, and specificity testing with environmental isolates were steps taken to validate the function of the assay with environmental samples. Luminex response to cultures and to environmental samples was consistent with sequencing results, suggesting that the technology has the potential to simultaneously detect multiple targets for coastal water quality applications, particularly as progress is made to efficiently extract DNA from water and sediment matrices.  相似文献   

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