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1.
We compared the genetic diversity of three dominant myctophid fishes in the North Pacific Ocean that have different diel vertical migration patterns on the basis of the nucleotide sequences of the mitochondrial gene for cytochrome b. No genetic structure was detected for each of these three species. The genetic diversity progressively increased for Diaphus theta, a diel migrant species showing clear diel vertical migration; Stenobrachius leucopsarus, a semi-diel migrant, in which only part of the population migrates vertically; S. nannochir, a non-diel migrant. All three species were suggested to have experienced a recent, sudden population expansion. Interspecific differences in genetic diversity might be attributable to differences in the degree of population size reduction during the glacial periods; this degree in turn corresponds to the energy demand of the fishes.  相似文献   

2.
To examine the possibility that outer membrane proteins (OMP) of Synechococcus sp. remain in seawater, we investigated the stability of OMPs in vitro and in situ. Some fractions prepared from Synechococcus sp. CSIRO-94 were treated with trypsin and proteinase K. Four tightly bound OMPs were separated from Synechococcus. We designated the two major OMPs of 52 kDa and 48 kDa as Omp52Sy and Omp48Sy, respectively. Degradation of the OMP in natural seawater was monitored in microcosms to which intact Synechococcus cells and outer membrane (OM) were added. Omp52Sy and Omp48Sy were the most stable against trypsin and proteinase K among the OMPs when they were embedded in the OM. However, in the microcosm experiment using intact cells, Omp52Sy and Omp48Sy were detected in the particulate fraction only during the first 4 days, after which they could not longer be detected. Omp52Sy and Omp48Sy were the most stable proteins among the Synechococcus OMPs in vitro, but they might be degraded in situ. This indicates that stability of Synechococcus porin differs depending on complex formation with other membrane molecules, which might cause different preservation of microbial membrane proteins in the dissolved protein pool in the ocean. This study suggests that Gram negative bacterial OM with thin peptidoglycan forms a lipid bilayer that proptects OMP, but Synechococcus OM with thick peptidoglycan cannot form a lipid bilayer. The incomplete bilayer might not be able to protect from protease attack in the natural environment.  相似文献   

3.
Based on new and existing data on oxygen isotopes, alkenone-surface seawater temperature trends, planktonic foraminifers, lithology, and clay mineral composition of piston cores, a distinct cooling event has been identified around 8,200 cal a b.p. in the middle Okinawa Trough, northwest Pacific. This corresponds to the 8,200 a b.p. cooling event recorded in many places of the Northern Hemisphere. During this event, the local temperature decreased by 1°C, and the δ18O value increased by 0.6‰. A strengthened Asian winter monsoon is the most probable cause for this event, which thus adds further credibility to the contention that we are dealing here with a global phenomenon.  相似文献   

4.
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5.
Three ecological and geographical forms of Luciogobius guttatus from the western and eastern coasts of the Korean Peninsula as well as the coasts of, Jeju Island which is located to the south of the peninsula are described and compared based on morphological characteristics. The environments of these three coastal areas are different, and the morphometrics show a clear pattern of differentiation and reveal the discreteness of the western, eastern and Jeju groups. In addition, a discriminant analysis (DA), a principal component analysis (PCA) of morphometric characteristics, and a t-test for meristic characteristics all show that the three populations of L. guttatus differ from each other. Three divergent lineages of L. guttatus are described with the possibility of niche differentiation between the western, eastern and Jeju coastal habitats. The standard length of eastern populations was greater than that of western and Jeju populations, and those with morphometric characteristics had a longer body. In addition, eastern populations had a higher number of vertebrae. We speculate that the characteristics of eastern populations evolved through the difference in tide level and gravel density, whereas the difference between western and Jeju populations is due to geographical distance.  相似文献   

6.
The food habits of the dominant myctophid Stenobrachius leucopsarus were examined in the central basin of the Bering Sea in relation to oceanographic conditions, in summer 2002 and 2003 and spring 2006. S. leucopsarus exhibited an ontogenetic and seasonal dietary shift. In spring, small fish (≤40 mm) preyed mainly on Neocalanus flemingeri/plumchrus whereas large fish fed mainly on Neocalanus cristatus. In summer, small fish preyed mainly on Metridia pacifica whereas large fish fed mainly on euphausiids (Thysanoessa spp.). In the summer of 2003, when water temperature in the epipelagic layer (≤100 m) was warmer, reflecting the prevalence of the Alaskan Stream, small-sized S. leucopsarus showed a higher stomach content index, perhaps reflecting the greater abundance of M. pacifica. Thus, the present study shows that the physical variability in the epipelagic layer affects not only diets but also feeding performance of micronekton.  相似文献   

7.
In the present study, we have investigated the conditions influencing encystment and excystment in the dinoflagellate Gyrodinium instriatum under laboratory conditions. We incubated G. instriatum in modified whole SWM-3 culture medium and in versions of modified SWM-3 from which NO3 −, PO4 3−, NO3 − + PO4 3−, or Si had been omitted and observed encystment. Percentage encystment was high in the media without N and without P, while the percentage encystment in the medium lacking both N and P was highest. Moreover, to investigate N or P concentration which induced the encystment, Gyrodinium instriatum was also incubated in media with different concentrations of inorganic N and P; the concentrations of NO2 − + NO3 − and PO4 3− were measured over time. The precursors of cysts appeared within 2 or 3 days of a decrease in NO2 − + NO3 − or PO4 3− concentration to values lower than 1 μM or 0.2 μM, respectively. When cysts produced in the laboratory were incubated, we observed excystment after 8–37 days, without a mandatory period of darkness or low temperature. We incubated cysts collected from nature at different temperatures or in the dark or light and observed excystments. Natural cysts excysted at temperatures from 10 to 30°C, in both light and dark, but excystment was delayed at low temperatures. These studies indicate that G. instriatum encysts in low N or P concentration and excysts over a wide temperature range, regardless of light conditions, after short dormancy periods.  相似文献   

8.
The seasonal abundance of the dominant dinoflagellate, Ceratium fusus, was investigated from January 2000 to December 2003 in a coastal region of Sagami Bay, Japan. The growth of this species was also examined under laboratory conditions. In Sagami Bay, C. fusus increased significantly from April to September, and decreased from November to February, though it was found at all times through out the observation period. C. fusus increased markedly in September 2001 and August 2003 after heavy rainfalls that produced pycnoclines. Rapid growth was observed over a salinity range of 24 to 30, with the highest specific rate of 0.59 d−1 measured under the following conditions: salinity 27, temperature 24°C, photon irradiance 600 μmol m−2s−1. The growth rate of C. fusus increased with increasing irradiance from 58 to 216 μmol m−2s−1, plateauing between 216 and 796 μmol m−2s−1 under all temperature and salinity treatments (except at a temperature of 12°C). Both field and laboratory experiments indicated that C. fusus has the ability to grow under wide ranges of water temperatures (14–28°C), salinities (20–34), and photon irradiance (50–800 μmol m−2s−1); it is also able to grow at low nutrient concentrations. This physiological flexibility ensures that populations persist when bloom conditions come to an end.  相似文献   

9.
To date no analytical solution of the pile ultimate lateral capacity for the general c–φ soil has been obtained. In the present study, a new dimensionless embedded ratio was proposed and the analytical solutions of ultimate lateral capacity and rotation center of rigid pile in c–φ soils were obtained. The results showed that both the dimensionless ultimate lateral capacity and dimensionless rotation center were the univariate functions of the embedded ratio. Also, the ultimate lateral capacity in the c–φ soil was the combination of the ultimate lateral capacity (f c ) in the clay, and the ultimate lateral capacity (f φ ) in the sand. Therefore, the Broms chart for clay, solution for clay (φ=0) put forward by Poulos and Davis, solution for sand (c=0) obtained by Petrasovits and Awad, and Kondner’s ultimate bending moment were all proven to be the special cases of the general solution in the present study. A comparison of the field and laboratory tests in 93 cases showed that the average ratios of the theoretical values to the experimental value ranged from 0.85 to 1.15. Also, the theoretical values displayed a good agreement with the test values.  相似文献   

10.
Seven Skeletonema species were identified at one station in the industrial harbor Dokai Bay, Japan, in October 2007 and then monthly from January 2008 to December 2009 by morphological scanning electron microscopy observations and molecular analyses of mainly the large subunit (LSU) rDNA. We refer to one species identified as S. dohrnii using LSU rDNA, but as S. marinoi using the small subunit (SSU) rDNA and consequently we use the term S. marinoi-dohrnii complex. This is the first time that S. ardens, S. costatum s.s., and S. pseudocostatum have been recorded in Japan and that S. menzelii and S. tropicum have been identified by molecular methods in Japan. The S. marinoi-dohrnii complex was isolated with high frequency all year-round, and S. japonicum was also isolated with high frequency but not so in summer. S. Tropicum was isolated from September to December, but S. ardens and S. costatum s.s. were isolated only when the water temperature exceeded 20°C. S. pseudocostatum bloomed just once, in summer, but S. menzelii was isolated in May and again in October. The continuous year-round occurrence of S. costatum s.l. in the bay is supported by the succession of these seven species. Six of the species, except S. menzelii, were important components of algal blooms in the bay. One to four species of Skeletonema were isolated every month. Monthly species diversity was higher when S. ardens, S. costatum s.s., and S. tropicum that are usually associated with tropical or subtropical waters, were isolated. In general, species diversity of the genus Skeletonema was very high in Dokai Bay.  相似文献   

11.
The nitrogenase activities of cyanobacteria of the genus Trichodesmium are strictly light-dependent, although they do not develop heterocysts. Recently, the development of heterocyst-equivalent cells (diazocytes) was suggested in Trichodesmium spp. However, no cells with a similar appearance to diazocytes could be found in Trichodesmium sp. NIBB1067. An immunocytochemical analysis of nitrogenase in individual cells was performed using polyclonal antibodies generated from DNA fragments of genes encoding the Fe-protein and the α-and the β-subunit of the MoFe-protein of nitrogenase of Trichodesmium sp. NIBB1067. Visualization of the antibody binding was carried out using a horseradish peroxidase-conjugated secondary antibody and a chromogenic substrate, 3,3′-diaminobenzidine, to avoid the masking effects caused by the bright autofluorescence emitted by Trichodesmium cells. Nitrogenase proteins were detected in almost all the cells (always higher than 95% of total cells) grown under nitrogen-limited conditions. These results indicate that Trichodesmium sp. NIBB1067 does not differentiate heterocyst-equivalent cells.  相似文献   

12.
We measured the ammonium excretion, phosphate excretion and respiration rates of the scyphomedusa Aurelia aurita from Ondo Strait, in the central part of the Inland Sea of Japan, at 28 and 20°C. The rates measured at 28°C were converted to those at 20°C using the Q10 values, i.e. 1.56, 1.57 and 2.80, for ammonium excretion, phosphate excretion and respiration rates, respectively. The composite relationships between metabolic rates and wet weight of a medusa (WW, g, range 11–1330 g) at 20°C were expressed by the following allometric equations. For ammonium excretion rate (N, μmoles N medusa−1d−1): N = 0.497WW 1.09, phosphate excretion rate (P, μmoles P medusa−1d−1): P = 0.453WW 0.84, and respiration rate (R, μmoles O2 medusa−1d−1): R = 96.9WW 1.06. Mean O:N ratios (i.e. atomic ratios of 16.9 and 11.0 at 28 and 20°C, respectively) indicated that the metabolism of A. aurita medusae was protein-dominated. These metabolic parameters enabled us to estimate the nitrogen and phosphorus regeneration rates of an A. aurita medusa population typical of early summer in the Ondo Strait (means of water temperature, medusa individual weight and population biomass: 20°C, 200 g WW and 50.8 g WW m−3, respectively). Regenerated nitrogen and phosphorus were equivalent to 10.0 and 21.6% of phytoplankton uptake rates, respectively, nearly twice that estimated for mesozooplankton, demonstrating that A. aurita medusae are key components of the plankton community, influencing the trophic and nutrient dynamics in the Ondo Strait during early summer.  相似文献   

13.
Studies of macrophytes in the coastal zone of the Artic Seas, including the White Sea, have shown the essential role of these algae in the activity of the coastal half-latitude ecosystems. In summer, during the macrophyte reproduction period, a great number of reproduction products are released into the water. For a short time, this considerably affects the ratio of the nanoplankton in the populations that inhibit the vast and shallow coastal areas. At different coastal sites in Chernorechenskaya Inlet, Kadalaksha Bay, during the period of intensive reproduction of Ascophyllum nodosum and Fucus vesiculosus, 42 plankton samples were collected in 2005. During this period the concentration of antherozoids in the water reached 55000 cells/ml (216 mg C/m3). The number of eggs was within the range of 0.05–0.7 cells/ml. The proportion of antherozoids in the total biomass of nanoplankton varied at different coastal sites from 0.37 to 99%, with a mean of 46% for the reproduction period of A. nodosum, and only 7% for the reproduction period of F. vesiculosus. As was shown by counts of F. vesiculosus female gametes in sedimentation traps, 1 m2 of the macrophyte bed (assuming 100% coverage) produces 18000–108000 eggs per day (0.33–2 mg C). The calculated flux of the reproductive material from the brown algae beds to the coastal water shows good agreement with the sample counts.  相似文献   

14.
Data from satellite altimeters and from a 13-month deployment of in situ instruments are used to determine an empirical relationship between sea-level anomaly difference (SLA) across the Kuroshio in the East China Sea (ECS-Kuroshio) and net transport near 28°N. Applying this relationship to the altimeter data, we obtain a 12-year time series of ECS-Kuroshio transport crossing the C-line (KT). The resulting mean transport is 18.7 ± 0.2 Sv with 1.8 Sv standard deviation. This KT is compared with a similarly-determined time series of net Ryukyu Current transport crossing the O-line near 26°N southeast of Okinawa (RT). Their mean sum (24 Sv) is less than the mean predicted Sverdrup transport. These KT and RT mean-flow estimates form a consistent pattern with historical estimates of other mean flows in the East China Sea/Philippine Basin region. While mean KT is larger than mean RT by a factor of 3.5, the amplitude of the KT annual cycle is only half that of RT. At the 95% confidence level the transports are coherent at periods of about 2 years and 100–200 days, with RT leading KT by about 60 days in each case. At the annual period, the transports are coherent at the 90% confidence level with KT leading RT by 4–5 months. While the bulk of the Kuroshio enters the ECS through the channel between Taiwan and Yonaguni-jima, analysis of satellite altimetry maps, together with the transport time series, indicates that the effect of mesoscale eddies is transmitted to the ECS via the Kerama Gap southwest of Okinawa. Once the effect of these eddies is felt by the ECS-Kuroshio at 28°N, it is advected rapidly to the Tokara Strait.  相似文献   

15.
In this study, the dB difference and characteristics of krill swarms inhabiting Subarea 48.1, which includes the west and south of the South Shetland Island and the Elephant Island peripheries, were estimated to distinguish Antarctic krill, using acoustics. From April 13 to 24, 2016, acoustic data were collected along 24 survey lines using the frequencies 38 and 120 kHz, and middle trawling was performed at 7 stations. Using the difference between the dB values of two volume backscattering strength (Sv) frequencies (38 and 120 kHz), a clear acoustic distinction could be made between Antarctic krill (4.9 to 12.0 dB) and fish (?4.0 to ?0.2 dB). The distributions and mean Sv of krill swarms in the Elephant Island peripheries and south of South Shetland Island were higher than those in the west of South Shetland Island. The mean length/ height ratio of krill swarms in the west of the South Shetland Island (64.5) was higher than that in the south (35.9) and the Elephant Island peripheries (33.8), with the length of the aggregations exceeding their height. Most krill swarms were distributed between the surface layer (less than 10 m below sea level) and within 200 m of water depth. These results are expected to serve as baseline data for evaluating krill density and biomass by distinguishing them from fish, using acoustics.  相似文献   

16.
In our attempt to characterize the interaction of trophic coupling between Synechococcus and pigmented nanoflagellates (PNFs), successive size-fraction experiments were performed at a coastal station on the northeast coast of Taiwan from June, 2005 to January, 2006. By estimating the growth rate and grazing rate of Synechococcus in the presence of nanoflagellates of different sizes, we truncated the food web by removing organisms with different body sizes (<2 μm, <5 μm, <10 μm, and <20 μm). The growth rates of Synechococcus ranged from −0.016 to 0.051 h−1 during the experimental period, suggesting that temperature was a primary mechanism controlling Synechococcus growth. In addition to size and relative biomass of pigmented nanoflagellates and Synechococcus, it is suggested that community structures played an important role in trophic link. Furthermore, we conclude that the trophic cascading effect in the northeast coast of Taiwan includes: 1) high grazing rates at night in the warm season; 2) the Synechococcus biomass generally exceeds the grazing threshold (6 × 104 cells mL−1); and 3) the biomass ratio of <5 μm PNFs to >5 μm PNFs should be 1:1 to 2:1.  相似文献   

17.
The rock bream, Oplegnathus fasciatus, is a common rocky reef game fish in East Asia and recently has become an aquaculture species. Despite its commercial importance, the population genetic structure of this fish species remains poorly understood. In this study, 163 specimens were collected from 6 localities along the coastal waters of Korea and China and their genetic variation was analyzed with mtDNA COI sequences. A total of 34 polymorphic sites were detected which determined 30 haplotypes. The genetic pattern reveals a low level of nucleotide diversity (0.04 ± 0.003) but a high level of haplotype diversity (0.83 ± 0.02). The 30 haplotypes are divided into two major genealogical clades: one that consists of only Zhoushan (ZS, East China Sea) specific haplotypes from the southern East China Sea and the other that consists of the remaining haplotypes from the northern East China Sea, Yellow Sea, Korea Strait, and East Sea/Sea of Japan. The two clades are separated by approximately 330~435 kyBP. Analyses of AMOVA and Fst show a significant population differentiation between the ZS sample and the other ones, corroborating separation of the two genealogical clades. Larval dispersal and the fresh Yangtze River plume are invoked as the main determining factors for this population genetic structure of O. fasciatus. Neutrality tests and mismatch distribution analyses indicate late Pleistocene population expansion along the coastal waters of Korea and China approximately 133–183 kyBP during which there were periodic cycles of glaciations and deglaciations. Such population information needs to be taken into account when stock enhancement and conservation measures are implemented for this fisheries species.  相似文献   

18.
In a study of the age and growth of Sillago aeolus, a total of 414 fish were collected from December 2000 to March 2002, around Okinawa Island. Ages of 403 specimens were determined by otoliths. Monthly changes in the percentage of otoliths with an opaque zone in the outer margin indicated that an opaque ring formed once a year, occurring between February and May. The rings can therefore be defined as annual rings. Ages of females were estimated to be 0+ to 4+, while those of males were 0+ to 2+. The von Bertalanffy growth curves were well fitted to age-standard length data of males and females, as follows: Male: Lt = 209.6 [1 − exp{−0.70 (t + 0.58)}]. Female: Lt = 297.7 [1 − exp{−0.42 (t + 0.61)}].  相似文献   

19.
The intercellular distribution of nitrogenase was detected in a natural population of Trichodesmium spp. collected from Shitaba Bay, Uwajima, Ehime Prefecture, Japan. Polyclonal antibodies raised against the Fe-protein and the MoFe-protein (α-subunit) of nitrogenase were used as probes. Visualization of the antibody binding to the nitrogenase was performed using horseradish peroxidase-conjugated secondary antibody and the chromogenic substrate, 3,3′-diaminobenzidine tetrahydrochloride, to avoid the masking effects caused by the bright autofluorescence emitted by the Trichodesmium cells. Nitrogenase proteins were detected in 66 to 81% of the trichomes. More than 77% of the cells were immunostained in individual nitrogenase-containing trichome. The data show natural populations of Trichodesmium spp. do not developed heterocyst-equivalent cells.  相似文献   

20.
A numerical experiment using a three dimensional level model was performed to clarify the mechanism generating a strong coastal current, Kyucho, induced by the passage of Typhoon 0406 around the tip of the Tango Peninsula, Japan in June 2004. Wind stress accompanied by Typhoon 0406 was applied to the model ocean with realistic bottom topography and stratification condition. The model well reproduced the characteristics of Kyucho observed by Kumaki et al. (2005), i.e., the strong alongshore current with maximum velocity of 53 cm s−1 and its propagation along the peninsula with propagation speed of about 0.6 m s−1 one half-day after the typhoon’s passage. Coastal-trapped waves (CTW) accompanied by downwelling were induced along the northwest coast of the peninsula by the alongshore wind stress. The energy density flux due to the CTW flowed eastward along the coast, and indicated scattering of the CTW around the eastern coast of the peninsula. In addition, significant near-inertial internal gravity waves were also caused in the offshore region from the west of the Noto Peninsula to the north of the Tango Peninsula by the typhoon’s passage. The energy flux density of the near-inertial fluctuations flowed southward off the Fukui coast, and part of the energy flux was trapped on the tip of the Tango Peninsula, flowing with the coast on its right. It was found that the strong current, Kyucho, at the northeastern tip of the Tango Peninsula was generated by superposition of the near-inertial internal gravity waves and subinertial CTW.  相似文献   

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