共查询到20条相似文献，搜索用时 46 毫秒
1.
【目的】研究探讨造礁石珊瑚自然海区断枝培育技术的条件和方法。【方法】在深圳大鹏新区大澳湾海区海底搭建海区断枝培育系统,并在系统上培育膨胀蔷薇珊瑚(Montiporaturgescens)、霜鹿角珊瑚(Acropora pruinosa)、盾形陀螺珊瑚(Turbinaria peltata)、十字牡丹珊瑚(Pavona decussata)和澄黄滨珊瑚(Porites lutea)等5种造礁石珊瑚的断枝(每种珊瑚100株),开展为期12个月的实验。【结果】5种珊瑚在深圳市大鹏新区大澳湾海域的断枝培育系统上培育12个月后,5种造礁石珊瑚断枝存活率分别为96%、93%、95%、85%和83%。珊瑚断枝生长指标测量结果显示,5种珊瑚中霜鹿角珊瑚生长速度最快,其断枝横向增长长度平均可达46.2mm;其次是膨胀蔷薇珊瑚,其断枝横向伸长长度平均可达38.2 mm;而澄黄滨珊瑚生长速度最慢,横向伸长长度都在24mm以下。【结论】在合适海区,使用海区石珊瑚断枝培育系统进行造礁石珊瑚培育,可改善其存活率和生长。 相似文献
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《广东海洋大学学报》2020,(5)
【目的】对珠海庙湾岛海域进行造礁石珊瑚分布及种类多样性调查。【方法】采用石珊瑚群落边界摸查和截线样条法开展石珊瑚群落的面积分布及生态资源调查。【结果与结论】造礁石珊瑚分布区共有14个,分布面积共42.33 hm~2,分布区主要集中在庙湾岛滃崖礁东、下风湾、庙湾岛西中、水坑湾、钳虫尾湾及庙湾岛东南近岸海域;珠海庙湾岛海域共有造礁石珊瑚11科23属50种及2个未定种,优势种为澄黄滨珊瑚、翼形蔷薇珊瑚和秘密角蜂巢珊瑚;庙湾岛活造礁石珊瑚覆盖率显著下降,珊瑚礁资源退化严重,其中水坑湾降幅达43.56%,下风湾及钳虫尾湾珊瑚覆盖率降低20%～30%。 相似文献
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对裸体方格星虫（Sipunculus nudus）、可口革囊星虫（Phascolosoma esculenta）和澳洲管体星虫（Siphonosoma australe）的线粒体16S rRNA、COⅠ和细胞色素b（Cyt b）基因片段序列进行比较,并对其系统发生进行了初步探讨.采用PCR方法得到总长度分别为531～544 bp（16S）、652～675 bp（COⅠ）和406～453 bp（Cyt b）的线粒体片段.片段碱基A＋T比例较高（16S rRNA基因58.3%,COⅠ基因56.9%,Cyt b基因59.5%）. 16S rRNA片段存在169个碱基变异位点（其中包括167个简约信息位点）和44个碱基插入/缺失,种内个体间变异较小;COⅠ片段有512个碱基（333个简约信息位点）存在变异,79个碱基插入/缺失;Cyt b片段存在347个碱基（318个简约信息位点）变异位点,16个碱基插入/缺失.数据分析结果支持3种星虫和环节动物的分类地位较近,与软体动物较远的分类观点.此外,裸体方格星虫与澳洲管体星虫之间亲缘关系较近（D=0.3159、03156、0.2361）.认为3种星虫线粒体16S rRNA、COⅠ和Cyt b基因在种间存在明显的多态性,证实了三种基因序列均普遍适用于星虫种及以上阶元的系统学分析. 相似文献
4.
《广东海洋大学学报》2017,(4)
通过ITS基因特异扩增测序,对大鹏半岛海域46种石珊瑚ITS基因片段序列进行了比较分析。结果表明,该片段序列长度在674~806碱基对(bp)之间,A、T含量在40%~55.5%之间,大部分物种的序列碱基A、T含量小于G、C含量。46种造礁珊瑚的平均遗传距离为0.261。采用邻位连接(NJ)和最小进化(ME)法分别构建15种复合型珊瑚的5.8S r DNA系统发育树和28种坚实型珊瑚的ITS2系统发育树,结果显示15种复合型珊瑚的5.8S r RNA序列系统进化聚类结果较符合传统形态学分类结果,而28种坚实型珊瑚的ITS2序列系统进化聚类结果与传统形态学存在较大的差异,提示石珊瑚表型的变异性可能对传统分类存在影响。 相似文献
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对裸体方格星虫(Sipunculus nudus)、可口革囊星虫(Phascolosoma esculenta)和澳洲管体星虫(Siphonosoma australe)的线粒体16S rRNA、COI和细胞色素b(Cytb)基因片段序列进行比较,并对其系统发生进行了初步探讨。采用PCR方法得到总长度分别为531～544bp(16S)、652～675bp(COI)和406～453bp(Cytb)的线粒体片段。片段碱基A+T比例较高(16S rRNA基因58.3%,COI基因56.9%,Cytb基因59.5%)。16S rRNA片段存在169个碱基变异位点(其中包括167个简约信息位点)和44个碱基插入/缺失,种内个体间变异较小;COI片段有512个碱基(333个简约信息位点)存在变异,79个碱基插入/缺失;Cytb片段存在347个碱基(318个简约信息位点)变异位点,16个碱基插入/缺失。数据分析结果支持3种星虫和环节动物的分类地位较近,与软体动物较远的分类观点。此外,裸体方格星虫与澳洲管体星虫之间亲缘关系较近(D=0.3159、0.3156、0.2361)。认为3种星虫线粒体16S rRNA、COI和Cytb基因在种间存在明显的多态性,证实了三种基因序列均普遍适用于星虫种及以上阶元的系统学分析。 相似文献
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利用线粒体16S rRNA基因和线粒体细胞色素氧化酶亚基Ⅰ(cytochrome oxidaseⅠ,COⅠ)基因片段初步研究钦州湾牡蛎(Ostrea)的物种组成。以特异引物进行PCR扩增,对扩增产物进行纯化、测序,分析表明,16S rRNA基因部分长度为413bp,COⅠ基因部分长度为535bp,2种牡蛎序列的碱基组成均显示出较高的A+T比例:16S rRNA基因59.8%;COⅠ基因60.5%。对位排序比较表明,16S rRNA片段种内个体间变异较小,存在7个变异位点,4种单倍型,其中包括5个转换位点突变,2个颠换位点突变;COⅠ片段有30个碱基存在变异,7种单倍型,其中包括16个转换位点突变,14个颠换位点突变。运用MEGA4软件计算出不同个体间的遗传距离,并构建了NJ和UPGMA系统树。香港牡蛎(Crassostrea hongkongensis)16S rRNA和COⅠ序列与白肉牡蛎的遗传距离均为0.000,有明巨牡蛎(Crassostrea ariakensis)16S rRNA和COⅠ序列和红肉牡蛎(red meat ostrea)的遗传距离分别为0.000和0.011,白肉牡蛎16S rRNA和COⅠ序列和红肉牡蛎之间的遗传距离分别为0.035和0.146。结果表明,钦州湾牡蛎分为2个不同的种,线粒体16S rRNA和COⅠ基因在种间存在明显的多态性,证实了16S rRNA和COⅠ基因序列适用于牡蛎的系统学分析。 相似文献
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通过PCR扩增直接测序得到10种徐闻石珊瑚线粒体Cyt b基因部分序列,分析其碱基组成和变异频率,并采用Neighbor-Joining和Maximum Parsimony法构建系统发育树。结果显示:序列中A+T比例为61.0%,G+C比例为39.0%,前者明显高于后者,碱基替换主要发生在密码子第3位;滨珊瑚科分类与传统分类存在一定差异,揭示传统形态学分类可能受珊瑚骨骼生长可塑性限制,造成分类不准确;刺柄珊瑚属与蜂巢珊瑚科各属亲缘关系较近。 相似文献
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【目的】分离鉴定大口黑鲈（Micropterus salmoides）肠道益生菌,评估分离菌用作饲料添加剂的益生潜力。【方法】采集大口黑鲈肠道样本,采用稀释涂布法分离细菌,经16S rRNA序列比对和生化试验鉴定种属,并进行抗逆性能、黏附能力、溶血活性和药敏特性等鉴定。【结果】从大口黑鲈肠道分离菌株84株,经测序、blast比对,筛选出3株同源性最高的菌株MS-1、MS-2、MS-3,分别鉴定为地衣芽孢杆菌（Bacillus licheniformis）、解淀粉芽孢杆菌（Bacillus amyloliquefaciens）和贝莱斯芽孢杆菌（Bacillus velezensis）。3株分离菌均为γ-溶血,不具有生物膜,疏水性50%～93.3%,自凝集性43.6%～69.2%;可在pH 2.0～12.0环境和5.0 g/L胆盐中存活;经高温处理后仍可生长;3株菌对大多数抗生素敏感。【结论】芽孢杆菌MS-1、MS-2和MS-3抗逆性高,黏附性能及饲料学安全性高,可作为大口黑鲈养殖中潜在益生菌。 相似文献
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黄喉拟水龟体表溃疡病原菌SG_(24)的分类鉴定 总被引：1，自引：0，他引：1
从患溃疡的黄喉拟水龟病灶中分离到一株病原菌SG24。对菌株SG24进行了常规生理生化测定和ATBExpression半自动细菌鉴定仪鉴定,并测定16S rRNA序列,分析其与相关细菌相应序列的同源性,构建了系统进化树。普通细菌学方法结果显示菌株SG24为黏质沙雷氏菌(Serratia marcescens)。以沙雷氏菌属的16S rRNA基因序列设计一对引物进行PCR扩增,获得了菌株SG24大小约950 bp的16S rRNA部分基因片段,测序结果显示菌株SG24与黏质沙雷氏菌同类,与已登录的黏质沙雷氏菌(S.marcescensDQ207558)的16S rRNA同源性大于99%。综合以上分类鉴定结果,确定菌株SG24属于沙雷氏菌属的黏质沙雷氏菌。药物敏感试验结果表明:菌株SG24对链霉素、庆大霉素、壮观霉素、强力霉素、卡那霉素、阿米卡星、诺氟沙星、氧氟沙星和复方新诺明敏感。 相似文献
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《广东海洋大学学报》2016,(4)
通过16SrRNA基因特异扩增测序,对惠州大亚湾大辣甲岛和小辣甲岛附近海域14种造礁石珊瑚16SrRNA基因片段序列进行了比较分析。结果表明,该片段序列的平均A+T含量为63.5%,所有物种的序列碱基AT含量大于GC含量,序列都处于高度饱和的状态。14种造礁石珊瑚的平均遗传距离为0.179。采用NJ、ML和ME法构建系统发育树,结果显示蜂巢珊瑚科单独作为分支处于发育树的基部,而第二支分支包括菌珊瑚科、鹿角珊瑚科、滨珊瑚科和枇杷珊瑚科。裸肋珊瑚科的腐蚀刺柄珊瑚聚到了蜂巢珊瑚科中,与传统形态学分类存在差异,提示造礁石珊瑚表型的变异性可能对传统分类存在影响。 相似文献
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Based on the hydrographic data in austral summer during the 22nd Antarctic Expedition of China(2005/2006),some features can be found about the northern margin of Emery ice shelf as follows.The heat content in the surface layer(0-50 m) at the eastern end and the western end of the ice-shelf margin is much higher than that at the middle.The upper mixing-layer depth and the seasonal thermocline depth at the middle of the ice-shelf northern margin are much shallower than those at the both ends.However there is much less difference between the middle and the ends in the bottom layer.The remote sensing photos show that the inhomogeneity in the surface-layer water is closely related to the spatial distribution of the floes and polynia in the area. 相似文献
13.
Phylogenetic analysis based on 16S rDNA of 8 strains of cultivable bacteria isolated from Arctic sea-ice was studied.The results showed that strain BJ1 belonged to genus Planococcus,which was a genus of low mole percent G C gram-positive bacteria;strain BJ6 belonged to genus Burkholderia of β-proteobacteria and the rest 6 strain all belonged to γ-proteobacteria,of which strain BJ8 was a species of Pseudoalteromonas,strain BJ2-BJ5 and BJ7 were members of genus Psychrobacter.Phylogenetic analysis also indicated that bacteria of genus Psychrobacter of the isolates formed a relatively independent phylogenetic cluster in comparison with other bacteria belonged to genus Psychrobacter. 相似文献
14.
《极地研究(英文版)》2014,(3)
正1 Aims and Scope Advances in Polar Science(APS)is an international,peer-reviewed journal jointly sponsored by the Polar Research Institute of China and the Chinese Arctic and Antarctic Administration.APS is a comprchensive academic journal dedicated to presentation of multi-disciplinary achievements in Arctic and Antarctic expeditions and research.Its primary purpose is to publish achievements in fundamental research,applied 相似文献
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《山地科学学报》2014,(4):I0004-I0009
<正>AIMS AND SCOPE(Editorial Policy)The Journal of Mountain Science(JMS)is devoted to mountains and their surrounding lowlands-ecoregions of particular global importance,with a particular emphasis on the important highlands/mountains in the world,such as the Tibetan Plateau,the Himalayas,the Alps,the Andes,the Rockies and many other mountain ranges of our planet. 相似文献
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《山地科学学报》2014,(4)
<正>The Journal of Mountain Science(JMS),founded in2004,is an international English-language journal on mountain sciences.JMS is supervised by the Chinese Academy of Sciences(CAS),sponsored by the Chengdu Institute of Mountain Hazards and Environment,CAS,published by Science Press China,and distributed by Springer exclusively throughout the world(excluding Mainland China).The JMS is published bimonthly,fulltexted in SpringerLink and CNKI,indexed/abstracted by 相似文献
18.
References： 《极地研究(英文版)》2007,18(1):73-83
Using the NCEP/NCAR reanalysis monthly 500 hPa height data on a 2.5 latitude-longitude grid and 1°×1° sea ice data,the polar vortex area,intensity index and arctic sea ice area index are calculated respectively,and the meridional distribution,period variation and the abrupts in the long range trend are analyzed to study their relationship.The results show that the meridional distribution of sea ice and polar vortex h-ave distinctive difference,the relative positions of them are different in the eastern and western hemispheres,and exept they have periods of 4 months,quasi half year,quasi year,4-5 years and 10 years commonly,and each of them has its own respective variation as well.The sea ice area is decreasing apparently since 1980's,so is the polar vortex area,but their abrupt changge time are different totally.The area of sea ice and polar vortex has prominent positive correlation,but the relationship of sea ice intensity,polar vortex intensity,polar vortex area is complicated. 相似文献
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The ability of cell to modulate the fluidity of plasma membrane was crucial to the survival of microorganism at low temperature. Plasma membrane proteins, fatty acids and carotenoids profiles of Antarctic psychrophilc yeast Rhodotorula sp. NJ298 were investigated at -3 ℃, 0 ℃ and 8 ℃. The results showed that plasma membrane protein content was greater at -3 ℃ than that at 8 ℃, and a unique membrane polypeptide composition with an apparent molecular mass of 94.7 kDa was newly synthesized with SDS-PAGE analysis; GC analysis showed that the main changes of fatty acids were the percentage of unsaturated fatty acids (C18∶ 1 and C18∶ 2) and shorter chain saturated fatty acid (C10∶ 0) increased along with the decrease of the culture temperature from 8 ℃ to -3 ℃; HPLC analysis indicated that astaxanthin was the major functional carotenoids of the plasma membrane, percentage of which increased from 54.6±1.5% at 8 ℃ to 81.9±2.1% at -3 ℃. However the fluidity of plasma membrane which was determined by measuring fluorescence anisotropy was similar at -3 ℃, 0 ℃ and 8 ℃. Hence these changes in plasma membrane's characteristics were involved in the cellular cold-adaptation by which NJ298 could maintain normal plasma membrane fluidity at near-freezing temperature. 相似文献