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1.
Shiro Gohda 《Journal of Oceanography》1974,30(4):163-167
The content and the oxidation state of arsenic and antimony in coastal seawater were determined coupled with chemical separation and neutron activation method. The contents of arsenic and antimony in coastal seawater were estimated to be 1.56–2.73g As/l, 0.25–0.57g Sb/l. Most parts of these elements exist as soluble ionic species, passing through 0.45 pore-size membrane filter, and at surface, 80–97% of arsenic occurs as arsenate and 70–94 % of antimony may occurs as antimonate. The vertical distribution of arsenite and arsenate is almost constant from the surface to 1,200 m depth. However the distribution of Sb(III) and Sb(V) is much more complicated with depth. 相似文献
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为了优化蛏苗集约化平面流中间培育技术,研究了不同进水流速和苗种规格对缢蛏中间培育效果的影响,并分析了集约化平面流中间培育系统的水质状况。结果显示,不同进水流速对缢蛏稚贝生长影响显著,稚贝生长速率随进水流速增加而增加,但成活率下降。通过流速与成活率和体质量日增生长量的线性回归分析,估算0.163L/s为适宜的进水流速。在适宜流速和相同放苗重量下,大规格苗种(8万粒/kg)生长速度显著高于小规格苗种(18万粒/kg),但因为小规格组放苗数量多,小规格组单位面积质量较其高出23.72%。除低流速组以外,平面流中间培育过程对叶绿素a和铵态氮有良好的去除效果,去除率分别达到36.99%和3.88%以上,这表明平面流集约化中间培育在利用海水池塘水体进行苗种中间培育的同时,也起到了池塘养殖水体的净化作用。综合认为,在养殖密度0.5kg/m^2、流速0.163 L/s的培育条件下,可以保证水体自污染程度较低,缢蛏苗种生长较快,成活率在73.12%以上。 相似文献
4.
We examined the contribution of submarine groundwater discharge (SGD) to nutrient budgets in Hwasun Bay, Jeju Island, Korea in August 2009, October 2014, and May 2015. The concentrations of dissolved inorganic nitrogen (DIN) and dissolved inorganic phosphorus (DIP) in fresh groundwater were in the range of 285?716 μM and 2.3?3.2 μM, respectively, which were each 1?2 orders of magnitude higher than those in the bay seawater. The outer-bay seawater flowing into the bay was oligotrophic (2.9 ± 1.9 μM for DIN and 0.2 ± 0.3 μM for DIP). Nutrient budget calculations were performed for each season by accounting for submarine fresh groundwater discharge (SFGD) and water residence times. In August 2009 (DIN = 1.8 μM and DIN:DIP ratio = 4.6 for the outerbay water), DIN inputs from SFGD accounted for approximately 40% of the DIN inventory in the bay seawater. In October 2014 (DIN = 1.1 μM and DIP < 0.05 μM for the outer-bay water), DIP from SFGD accounted for approximately 100% of the DIP inventory in the bay seawater. In May 2015, mean concentrations of DIN and DIP in the bay seawater were 8.6 ± 12 μM and 0.11 ± 0.04 μM, respectively, with conservative behaviors in the bay seawater in association with excessive groundwater inputs. These results imply that SGD plays a critical but different role in nutrient budgets and stoichiometry in coastal waters off a volcanic island depending on open-ocean nutrient conditions. 相似文献
5.
A method for the determination of nanomolar concentrations of orthophosphate in oligotrophic seawater developed by Liang et al. (2007) has been modified to make it fully feasible for shipboard application and for faster sample throughput with minimized
sample volume. The technique is based on the flow injection method with solid phase extraction on a Sep-Pak C18 cartridge
and colorimetric detector. The Schlieren effect was minimized by rinsing the cartridge sequentially with 5 mL water and 2
mL 95% ethanol solution. With three micro pumps in parallel, savings of up to 80% in amount of reagents and 25% volume of
seawater samples could be achieved in comparison to the previous method. Variation of stopped flow time and sample loading
time gave 3 different standard curves, which corresponded to 3 linear ranges within 3.4 and 515 nM. The modified method permits
the analysis of samples over a wide range of concentrations, and has been successfully applied to shipboard determination
of trace orthophosphate in more than 200 seawater samples during a one-month cruise in the South China Sea. For seawater at
concentrations of 20.6, 82.5, 206.2 nM orthophosphate, the relative standard deviations (RSD) (n = 6), determined daily for 6 days on board ship were 4.45%, 4.73% and 6.75%, respectively. Five seawater samples collected
in the Station SEATS (South East Asia Time Series Station at 18°N, 116°E) were analyzed using the present method both on board
and in a land-based laboratory, as well as with the magnesium hydroxide-induced coprecipitation (MAGIC) method, and showed
no significant difference according to the statistical t-test. 相似文献
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A continuous flow method for the determination of ammonium concentration in seawater from a nanomolar to a micromolar level is described. To prevent spurious peaks derived from salinity difference, a gas-permeable hydrophobic membrane filter was used to separate the manifold into an outgassing section and an indophenol blue reaction section. The indophenol blue reaction section was adopted for colorimetric analysis and is equipped with a 1-m path length liquid capillary cell and a fiber-optic spectrometer, which is able to record the absorbance at multiple wavelengths. The minimum detection limit at wavelength 630 nm is 5.5 ± 1.8 nM, and the calibration curves are linear to at least 2,000 nM. In addition, the minimum detection limit at wavelength 530 nm was 13 ± 5.3 nM, and linear calibration curves were observed until at least 10,000 nM. The slopes of the calibration curves were similar for standards prepared using filtered seawater and ultrapure water. The ammonium concentration of the ultrapure water was similar to those of ion-exchanged water and unfiltered low-nutrient seawater, but was significantly lower than those of filtered seawater and solutions that contained sodium hydroxide. Therefore, ultrapure water is optimal for both blank and standard preparations because of its stable quality and availability. Given its large concentration range and the use of readily available blanks, this method is suitable for the determination of ammonium concentration and helps our understanding of ammonium dynamics in the ocean. 相似文献
8.
为分析海洋微表层这一特殊生境中的细菌类群,于2010—2011年4个航次对北黄海微表层和次表层海水中的总菌丰度、可培养细菌丰度和群落结构进行了分析。采用流式细胞仪测定总菌丰度,平板计数法测定可培养细菌丰度,PCR-16SrDNA分析可培养细菌的群落结构。结果表明,调查海域微表层海水总菌均值为1.76×106 cell/mL,次表层海水总菌均值为1.07×106 cell/mL。可培养细菌丰度范围是1.00×102~1.70×106 CFU/mL,微表层和次表层可培养细菌所占总菌数量的百分比分别为13.05%和0.45%。微表层对总菌的富集因子(EF)均值为2.02,可培养细菌的EF均值为74.16。PCR-16SrDNA序列分析结果表明,该海域可培养细菌分属变形菌门(Proteobacteria)(94.34%)、厚壁菌门(Firmicutes)(1.89%)、拟杆菌门(Bacteroidetes)(1.89%)和放线菌门(Actinobacteria)(1.89%)4个类群。本研究初步发现,微表层对细菌具有较强的聚集作用,尤其对可培养细菌聚集作用更为明显。微表层中可培养细菌的群落结构与次表层有所不同,其种类丰富,来源和功能多样。由此可见,微表层独特的生境成就了其独特的微生物类群,其生态功能有待于进一步研究。 相似文献
9.
电解富集-液体闪烁计数法测量北极海水中氚活度 总被引：1，自引：1，他引：0
A method of measuring the tritium in seawater based on electrolytic enrichment and ultra-low background liquid scintillation counting techniques was established. The different factors influencing the detection limit were studied, including the counting time, the electrolytic volume of the seawater samples, the selection of background water, scintillation solution and their ratio. After optimizing the parameters and electrolyzing 350 mL volume of samples, the detection limit of the method was as low as 0.10 Bq/L. In order to test the optimization of system for this method, of the 84 seawater samples collected from the Arctic Ocean we measured, 92% were above the detection limit(the activity of this samples ranged from 0.10 Bq/L to 1.44 Bq/L with an average of(0.30±0.24) Bq/L).In future research, if we need to accurately measure the tritium activity in samples, the volume of the electrolytic samples will be increased to further reduce the minimum detectable activity. 相似文献
10.
《Marine Chemistry》2007,103(1-2):122-130
Phosphomolybdenum blue (PMB) paired with cetyltrimethylammonium bromide (CTAB) can be extracted using a solid phase extraction technique on C18 sorbent. Based on this, a novel on-line solid phase extraction method coupled with flow injection (FI) analysis and colorimetric detection has been established to determine nanomolar level orthophosphate in seawater. A stopped flow technique was employed to assure the complete formation of the PMB–CTAB compound, which was sequentially extracted on an in-line Sep-Pak C18 cartridge. The adsorbed PMB–CTAB can be rapidly eluted by 0.56 mol/L H2SO4 in ethanol, and determined with a spectrophotometer at 700 nm. Experimental parameters, including reaction temperature, sample loading flow rate, stopped time and eluting flow rate, were optimized throughout the experiments based on univariate experimental design. The results show that reaction temperature and stopped time were the major factors affecting the formation of PMB–CTAB. Silicate concentration up to 5000 times higher than that of orthophosphate would not interfere with the determination of orthophosphate. Using artificial seawater with salinity of 35 as a matrix under the optimized conditions, the standard curve shows a linear range between 3.2 and 48.5 nmol/L, and the recovery and the detection limit of the proposed method are 96.4% and 1.57 nmol/L, respectively. The relative standard deviation (RSD) (n = 8), which was determined daily for 8 days, was 4.52% for the artificial seawater at a concentration of 32.4 nmol/L orthophosphate. Two typical seawater samples were analyzed using both the proposed method and the MAGnesium hydroxide-Induced Coprecipitation (MAGIC) method. The results of the two methods show no significant difference using the t test. Compared to the MAGIC method, the proposed method has the advantage of being more sensitive, faster, sample saving and easy for on-line analysis. 相似文献
11.
Modified Method for the Analysis of Nitrogen Isotopic Composition of Oceanic Nitrate at Low Concentration 总被引：1，自引：0，他引：1
A method for the determination of the δ15N of nitrate in seawater described by Cline and Kaplan (1975) has been modified for application to low-level nitrate samples.
We have minimized the reagent blank problem by replacing the Devarda's alloy with an aluminum reagent, and have also established
a procedure that yields quantitative (93 ± 2%) extraction of nitrogen even at low nitrate levels. Though the amounts and the
δ15N of the blank N varied from one reagent set to another, with these modifications, an overall N blank was reduced to approximately
0.80 ± 0.33 μmole N having an estimated δ15N value of −1.8‰. After blank and yield corrections, the measured isotopic composition of nitrate differed by approximately
0.1‰ from the actual value while the precision was within ±0.2‰ at the 1.25 μM level. The modified procedure was applied to
seawater samples collected from the equatorial Pacific in order to compare the N blanks in field samples with those derived
from laboratory experiments. The results support the suitability of the modified approach for isotopic analysis of oceanic
nitrate in shallow water.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
12.
Chemical extraction techniques show that the majority of the arsenic in North Atlantic deep-sea sediments is associated with an iron phase compositionally similar to that found in deep-sea ferromanganese nodules (As/Fe ～ 11 · 10?4) and is probably of seawater origin. Some sediments also contain As associated with Fe oxides produced by continental weathering. A minority (～8%) of the arsenic is of detrital origin but is not associated with Fe or Mn oxides; it has a content (1.7 ppm) similar to the average crustal abundance. In the Eastern Mediterranean Sea, near-shore sediments contain As associated with land-derived Fe oxides (As/Fe ～ 2 · 10?4), but As/Fe ratios increase to ～ 13 · 10?4 in deep-sea sediments as the contribution of seawater derived arsenic becomes dominant. Arsenic is enriched in metalliferous sediments (As/Fe ～ 20?50 · 10?4) but As/P ratios of metalliferous sediments, deep-sea ferromanganese nodules and deep-ocean water are all similar. Although a hydrothermal contribution cannot be discounted, it is likely that the arsenic is also of seawater origin, suggesting that hydrothermal iron oxyhydroxides remove As more efficiently from seawater than do iron phases (goethite) in deep-sea sediments and nodules. Arsenic accumulates in deep-sea sediments (～ 6 μg cm?2 10?3 yr?1) at sediments (～ 120 μg cm?2 10?3 yr?1) at rate sufficient to balance river input input (～3 · 1010 g yr?1). These estimates give an oceanic residence time for arsenic of 1–2 · 105 yr. 相似文献
13.
海水中总氮和总磷的同时测定 总被引：24，自引：0，他引：24
早期总氮和总磷的过硫酸钾法氧化分析分别是在碱性和酸性介质中进行［１,２］,样品分别保存,然后分别氧化测定,工作较繁琐。１９７７年Ｋｏｒｏｌｅｆｆ用过硫酸钾氧化法同时氧化水体中的总氮和总磷,使样品的保存和氧化分析工作简化。１９８１年Ｖａｌｄｅｒｒａｍａ对该方法测定天然海水中的总氮和总磷做了进一步研究,总氮和总磷同时氧化后,水样中的硝酸盐和磷酸盐用手工分别进行分析。Ｗａｌｓｈ１９８９年和Ｋａｒｌ等１９９３年曾采用ＵＶ光氧化法对海水中总溶解氮和总溶解磷进行同时氧化后,用自动分析对氧化后的无机氮和磷进行… 相似文献
14.
A chemiluminescent technique for the determination of nanomolar concentrations of nitrate and nitrite in seawater 总被引：1，自引：0，他引：1
C. Garside 《Marine Chemistry》1982,11(2):159-167
A chemiluminescent analysis technique for the determination of nanomolar quantities of nitrate, nitrate plus nitrite or nitrite alone in seawater is described. The method depends on the selective reduction of these species to nitric oxide which is then determined by its chemiluminescent reaction with ozone, using a commercial nitrogen oxides analyzer. The necessary equipment is compact and sufficiently sturdy to allow shipboard use. A precision of ±2 nM is claimed with analytical rates of 10–12 samples h?1, and modifications are discussed to allow doubling the analytical rate. 相似文献
15.
Sensitive methods for the determination of arsenate and particulate arsenic (PAs), as well as phosphate and particulate phosphorus (PP), in seawater are described. The method for arsenate and phosphate was established by applying automated liquid waveguide spectrophotometry. Because the reaction time for the formation of the arsenate-molybdate complex is longer than that for the phosphate-molybdate complex, a long Teflon tube submerged in a heating bath was installed in the conventional phosphate flow system. The arsenate was quantified as the difference between absorbances of molybdenum blue dyes with (only phosphate) and without (phosphate + arsenate) arsenate reduction treatment. Contamination was observed in the reagent for arsenate reduction and must be corrected. Linear dynamic ranges up to 1000 nM were confirmed for arsenate and phosphate. The detection limits for arsenate and phosphate were 5 and 4 nM, respectively. Freezing was a reliable sample preservation technique for both arsenate and phosphate. The method for PAs and PP was established by combining conventional persulfate oxidation of PP and the automated liquid waveguide spectrophotometry of arsenate and phosphate. The digestion efficiencies of organic As analogs were >93%. Contamination in the glass fiber filter was negligible. Field tests confirmed that the coefficients of variation (CVs) of 10–19 nM arsenate and 4–151 nM phosphate were 7–20 and 1–25%, respectively, while the CVs of 0.9 nM PAs and 10.2 nM PP were 11 and 4%, respectively. 相似文献
16.
A thorough understanding of the biogeochemical cycling of trace metals in the ocean is crucial because of the important role these elements play in regulating metabolism in marine biotas and thus, the climate. However, a precise and accurate analysis of trace metals in seawater is difficult because they are present at extremely low concentrations in a high salt matrix. In this study, we report an analytical method for the preconcentration and separation of six trace metals, Fe, Ni, Cu, Zn, Cd and Pb, in seawater using a seaFAST automatic solid-phase extraction device, analysis by a triple quadrupole collision/reaction technique with inductively coupled plasma mass spectrometry (ICP-MS), and quantification by the isotope dilution technique. A small volume (10 mL) of seawater sample was mixed with a multi-element isotope spike and subjected to seaFAST procedures. The preconcentrate solution was then analyzed using the optimized collision/reaction cell mode of ICP-MS, with NH3 gas for Fe and Cd with a flow rate of 0.22 mL/min and He for Ni, Cu, Zn and Pb with a flow rate of 4.0 mL/min. The procedure blanks were 130 pmol/L, 3.0 pmol/L, 6.8 pmol/L, 37 pmol/L, 0.29 pmol/L and 0.42 pmol/L, for Fe, Ni, Cu, Zn, Cd and Pb, respectively. The method was validated using five reference materials (SLRs-6, SLEW-3, CASS-6, NASS-7 and GEOTRACE-GSC), and our results were consistent with the consensus values. The method was further validated by measuring full-water-column seawater samples from the subtropical Northwest Pacific Ocean, and our results demonstrated good oceanic consistency. 相似文献
17.
春季水华对南黄海总溶解态无机砷生物地球化学行为的影响 总被引：1，自引：0，他引：1
利用氢化物发生-原子荧光光谱法(HG-AFS)对2007年3月30日至4月23日南黄海海域总溶解态无机砷(TDIAs,[TDIAs]=[As5+]+[As3+])的含量进行了测定,其中针对水华中心区域(BM1站)进行了25h的连续观测,以探讨春季水华对有毒类金属元素砷的生物地球化学行为的影响。结果表明,TDIAs的浓度范围为7.9~22.3nmol/L,平均值为(17.8±1.9)nmol/L。TDIAs在南黄海的分布主要表现为由近岸向外海逐渐升高的趋势,最大值出现在南部海域底层海水中。近岸海域表、底层TDIAs的含量相当,而中、南部海域由于存在明显的密度跃层,表、底层TDIAs的浓度具有显著性差异。2007年3月31日至4月1日研究区域西南部受到沙尘天气和降雨的影响,表层海水中TDIAs的含量显著升高。研究区域中、南部海域在观测期间暴发了典型的黄海春季水华,通过大面观测和对重点区域的连续观测可以发现,水华期间TDIAs的分布和磷酸盐类似,与Chl a呈现出较好的负相关关系(r=0.51,P0.05,n=39)。经初步计算,浮游植物水华对10m以上表层水体中TDIAs的清除量约为2.4nmol/L,占表层保有量的15%左右。通过箱式模型计算得出黄海TDIAs的停留时间约为(18.2±8.5)a,远远低于大洋。通过对该海域砷、磷摩尔比值的计算可以发现,南黄海砷、磷摩尔比值约为大洋中的20倍左右,这可能会引起浮游生物对砷酸盐的大量吸收和转化,从而带来潜在的生态危机,需要引起足够的重视。 相似文献
18.
黄、渤海二甲基硫化物的浓度分布与迁移转化速率研究 总被引：2，自引：1，他引：1
于2015年8-9月对黄、渤海海域进行现场调查，研究了海水中二甲基硫（DMS）、β-二甲巯基丙酸内盐（DMSP）、二甲亚砜（DMSO）的浓度分布、相互关系及影响因素，测定了DMS的生物生产与消耗、光化学氧化和海-气扩散速率，对DMS的迁移转化速率进行综合评价。结果表明:表层海水中DMS、溶解态DMSP（DMSPd）、颗粒态DMSP（DMSPp）、溶解态DMSO（DMSOd）和颗粒态DMSO（DMSOp）浓度的平均值分别为（6.12±3.01）nmol/L、（6.03±3.45）nmol/L、（19.47±9.15）nmol/L、（16.85±8.34）nmol/L和（14.37±7.47）nmol/L，整体呈现近岸高远海低，表层高底层低的趋势。DMS、DMSPd和DMSOp浓度与叶绿素（Chl a）浓度存在显著的相关性。表层海水中DMS光氧化速率顺序为:kUVA > kUVB > k可见，其中UVA波段占光氧化的70.8%。夏季黄、渤海微生物消耗、光氧化及海-气扩散对DMS去除的贡献率分别为32.4%、34.5%和33.1%，表明3种去除途径作用相当。黄、渤海DMS海-气通量变化范围为0.79~48.45 μmol/（m2·d），平均值为（11.87±11.35）μmol/（m2·d）。 相似文献
19.
Research was implemented from September 15 through October 4, 2011 in the Kara Sea along transects located southeastwards Novaya Zemlya, in the St. Anna Trough, the Yenisei River estuary, and the adjacent shelf. The concentration of chlorophyll a was the highest in the photic zone (0.05–2.30 mg/m3, on average, 0.80 ± 0.37 mg/m3). The maximal concentration of Chl a at most of the stations located in the water layer of 7–30 m. Integral primary production in the water column varied from 3.0 to 151.0 mg C/m2 per day, on average, 37.2 ± 36.6 mg C/m2 per day. The maximal rate of primary production at most of the stations has been observed for the surface layer of the water column. Within the upper mixed water layer, relative primary production was from 31 to 100% (on average, 77 ± 20%). The most productive zone was the waters along Yenisei transect. In the estuary and at the adjacent shelf, primary production was 50 mg C/m2 per day, exceeding the range observed for other areas by 1.5–2.0 times. The concentrations of silica and nitrogen together with light regime and water temperature were the major limiting factors affecting the primary production rate in the Kara Sea in autumn. 相似文献
20.
《African Journal of Marine Science》2013,35(2):223-228
South African abalone Haliotis midae farms utilise large volumes of seawater (c. 500–1 500 l s–1) and produce relatively dilute effluents that are potentially suitable for the integrated culture of other species. To test this hypothesis, a marine finfish, silver kob Argyrosomus inodorus, and a detritivorous polychaete, bloodworm Arenicola loveni loveni, were cultured in abalone farm effluent and the results compared to controls reared in unused seawater. The silver kob were fed a nutritionally complete pelleted diet whereas the bloodworm were placed in shallow tanks with a low water velocity that allowed suspended organic solids to settle for the detritivorous worms to feed on. Silver kob growth rate (0.48% body weight d–1; SE = 0.01%), mortality (1.8 ± 0.5%), feed conversion ratio (3.0 ± 0.2) and protein efficiency ratio (1.0 ± 0.1) did not differ significantly between the effluent and control treatments. Bloodworm reared in abalone effluent grew well on the particulate organic waste matter in the effluent (0.39% body weight gain d–1; SE = 0.07%), whereas those in the seawater control lost weight at 0.19 ± 0.04% body weight d–1 over the experimental period. Bloodworm mortality did not differ significantly between effluent (6 ± 3%) and unused seawater (11 ± 8%) treatments. The faster growth of bloodworm in the abalone farm effluent was ascribed to the higher deposition rate of enriched organic solids (182 ± 56 g m–2 d–1) compared with those grown in the seawater control (46 ± 13 g m–2 d–1). It was concluded that abalone farm effluent is potentially suitable for the culture of both bloodworm and silver kob. 相似文献