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1.
近年来海水富营养化导致近海海域大型海藻过量繁殖,为研究大型海藻与营养盐的相互关系,我们采用实验生态学的方法研究了4种大型海藻(孔石莼、小珊瑚藻、缘管浒苔、石花菜)单养和两两混养条件下的生长、营养盐(PO4-P和NO3-N)含量变化以及营养盐吸收利用情况。结果表明,单养条件下,孔石莼的湿重增长最多(46.4%),其相对增长率显著高于缘管浒苔、小珊瑚藻、石花菜。孔石莼+缘管浒苔、孔石莼+小珊瑚藻、孔石莼+石花菜混养体系中,孔石莼的相对增长率显著高于缘管浒苔、小珊瑚藻、石花菜,其他混养体系中两种大型海藻的相对增长率无显著差异。在单养条件下培养10d,海藻对PO4-P、NO3-N的利用率分别以孔石莼(85.4%)、缘管浒苔(72.6%)为最大,而石花菜对两种营养盐的利用率均最小(51.1%和57.1%)。在混养条件下,PO4-P、NO3-N的最高利用率分别出现在孔石莼+小珊瑚藻(86.3%)和缘管浒苔+小珊瑚藻(81.7%)体系中。无论单养还是混养,海藻对PO4-P和NO3-N的吸收速率都在第2d达到峰值。因此,大型海藻可以有效地去除营养盐,4种藻中,孔石莼和缘管浒苔分别是去除PO4-P和NO3-N的最佳大型海藻。本研究结果可以为利用大型海藻进行环境污染的治理提供支持。  相似文献   

2.
石莼属(Ulva)和浒苔属(Enteromorpha)绿藻的RAPD分析   总被引:9,自引:1,他引:8  
于1997年下半年在大连石油庙海区采集石莼、孔石莼、砺菜、缘管浒苔、肠浒苔、管浒苔、刚毛藻等7种海藻样品,采用RAPD技术对大连地区绿藻门石莼属和浒苔属进行了初步的分子系统学研究。所试60个引物中有32个经扩增得到了237个多态片段。应用PHYLIP软件包,按照UPGMA法和N-J法聚类分析的结果均表明,缘管浒苔(Enteromorpha linza)与石莼属有较近的亲缘关系,应深水探究其分类地位  相似文献   

3.
报道秦皇岛港区底栖海藻的群落结构、季节变化、生物量及优势种类等。通过4次定性采样指出:4月和6月的种类数高于8月和10月,群落组成都以红藻类为优势。平均生物量分布以金山咀最高,灯塔最低,各采样点的优势种是新煤码头为绿藻类的孔石莼、肠浒苔和尾孢藻;灯塔为红藻类的珊瑚藻、鸭毛藻及绿藻类的孔石莼和刺松藻,金山咀为孔石莼、刺松藻、萱藻和蜈蚣藻。  相似文献   

4.
为比较大型藻抑制赤潮藻的能力,研究了孔石莼、羊栖菜、长浒苔、马尾藻、蜈蚣藻和裙带菜的干粉末海水浸提液对中肋骨条藻的化感抑制作用并从长浒苔中分离出具有强抑制作用的组分。分析6种大型藻浸提液对中肋骨条藻的生长抑制率(Inhibitory rate,IR%)和120 h的半效应浓度(Effect concentration,EC50,120 h)发现,孔石莼、羊栖菜、长浒苔、马尾藻和蜈蚣藻的浸提液在浓度达到2.4 g/L时可在实验周期内使中肋骨条藻全部致死,抑制率达到100%,全部致死所需时间分别为96、120、96、144和144 h,当浓度达到4.8 g/L时,6种大型藻均可全部杀死中肋骨条藻,6种大型藻对中肋骨条藻的EC50,120 h分别为1.0、1.0、1.1、1.4、1.5和4.7 g/L。从长浒苔浸提液中分离的乙酸乙酯萃取组分具有强抑藻效果,受试条件下,其EC50值为0.08 mg/L。乙酸乙酯相气相色谱-质谱分析表明,组分至少包含14种物质,其中9-十八炔和邻苯二甲酸二异丁酯是含量最大的2种物质,各物质的抑藻活性尚需进一步研究。长浒苔具有很强的抑藻效果,具有应用于赤潮藻控制的潜力,研究结果为分离鉴定抑制中肋骨条藻的化感物质奠定了基础。  相似文献   

5.
六种大型藻浸提液对中肋骨条藻的抑制及活性成分分离   总被引:1,自引:0,他引:1  
为比较大型藻抑制赤潮藻的能力,研究了孔石莼、羊栖菜、长浒苔、马尾藻、蜈蚣藻和裙带菜的干粉末海水浸提液对中肋骨条藻的化感抑制作用并从长浒苔中分离出具有强抑制作用的组分.分析6种大型藻浸提液对中肋骨条藻的生长抑制率(Inhibitory rate,IR%)和120 h的半效应浓度(Effect concentration,EC50,120h)发现,孔石莼、羊柄菜、长浒苔、马尾藻和蜈蚣藻的浸提液在浓度达到2.4 g/L时可在实验周期内使中肋骨条藻全部致死,抑制率达到100%,全部致死所需时间分别为96、120、96、144和144 h,当浓度达到4.8 g/L时,6种大型藻均可全部杀死中肋骨条藻,6种大型藻对中肋骨条藻的EC50、120h分别为1.0、1.0、1.1、1.4、1.5和4.7 g/L.从长浒苔浸提液中分离的乙酸乙酯萃取组分具有强抑藻效果,受试条件下,其EC50值为0.08 mg/L.乙酸乙酯相气相色谱-质谱分析表明,组分至少包含14种物质,其中9-十八炔和邻苯二甲酸二异丁酯是含量最大的2种物质,各物质的抑藻活性尚需进一步研究.长浒苔具有很强的抑藻效果,具有应用于赤潮藻控制的潜力,研究结果为分离鉴定抑制中肋骨条藻的化感物质奠定了基础.  相似文献   

6.
大型海洋绿藻5.8S rRNA基因序列及系统发育分析   总被引:2,自引:0,他引:2       下载免费PDF全文
采用PCR方法,将扩增的产物直接测序,测定了孔石莼Ulva pertusa和浒苔Enteromorpha prolifeFa的5.8S rRNA基因序列,与肠浒苔、扁浒苔、缘管浒苔、礁膜、北极礁膜、袋礁膜和格氏礁膜等7种已知的大型海洋绿藻的同一基因序列合并进行比较,分析了核苷酸序列差异和碱基替换特点,并构建系统发生树。结果表明,浒苔和孔石莼的颠换率为0.690%,小于浒苔与肠浒苔、扁浒苔、缘管浒苔的颠换率;且浒苔与孔石莼之间的Kimura双参数距离也小于浒苔与肠浒苔、扁浒苔以及缘管浒苔之间的平均Kimura双参数距离。相对于浒苔属的其他物种,浒苔与孔石莼的亲缘关系更近。在构建的系统发生树中,缘管浒苔与孔石莼聚为一类,表现出与石莼属物种更近的亲缘关系。  相似文献   

7.
采用生物急性和慢性毒性实验的方法,研究了赤潮异弯藻对蒙古裸腹溞和日本新糠虾的毒性影响,并初步探讨了赤潮异弯藻导致二者较快死亡的机制。结果发现,赤潮异弯藻不仅对蒙古裸腹溞和日本新糠虾的存活有显著影响,而且使其个体变小、产幼前发育时间延长、总生殖量降低、平均存活时间缩短,种群数量增长受抑制。显微观察发现,蒙古裸腹溞能摄食赤潮异弯藻,但摄食后,其粪便紧紧地粘在蒙古裸腹溞的肠道末端;日本新糠虾不摄食赤潮异弯藻,但该藻粘附在其触须、附肢以及腹部、尾部的刚毛上,以及作为食物的卤虫幼体也被大量粘附在一起,聚集成团,或通过赤潮异弯藻粘附在日本新糠虾的附肢上。因此赤潮异弯藻很可能通过干扰蒙古裸腹溞和日本新糠虾正常的生理活动,而导致其快速死亡。  相似文献   

8.
本研究将环介导等温扩增技术(loop-mediated isothermal amplification,LAMP)与横向流动试纸条(lateral flow dipstick,LFD)的可视化检测方法结合,建立了扁浒苔(Ulva compressa)的LAMPLFD快速检测技术。该方法以扁浒苔的内转录间隔区(ITS1-5.8S-ITS2)序列为检测靶标,设计了3对特异性引物(其中,上游内引物由生物素标记)和1条异硫氰酸荧光素标记的探针。结果表明,LAMP最适反应温度为63°C,扩增时间为60 min,从核酸扩增到LFD结果判读需70 min。利用LAMP-LFD可特异性检出扁浒苔,对浒苔、曲浒苔、缘管浒苔和孔石莼等石莼属绿藻以及塔玛亚历山大藻、无纹环沟藻、东海原甲藻、锥状斯克里普藻和赤潮异弯藻等常见微藻的检测结果为阴性。该方法最低可检测到0.1 pg的扁浒苔基因组DNA,是以Uco ITS-F3和Uco ITS-B3为特异性引物的PCR方法的100倍。对实际样品的检测结果表明,LAMP-LFD方法检测扁浒苔与传统的形态学观察的结果一致。因此,该方法可快速、特异地检测出扁浒苔,而且操作简单,仪器设备依赖性低,有潜力成为扁浒苔现场检测的常规技术手段。  相似文献   

9.
流沙湾冬、春季大型海藻的微量元素分析   总被引:3,自引:0,他引:3  
采用微波消解法和AFA、ICP-MS对流沙湾2008年冬春季21种大型海藻的微量元素进行了分析。结果表明,As、Cu、Pb、Cr、Se、Fe、Mn在细江蓠体内含量最高,分别为8.90、34.35、24.26、68.12、12.04、26777.20、400.16mg/kg;Hg在海蕴体内含量最高,达6.90mg/kg;Zn在鼠尾藻体内含量最高,为277.37mg/kg;Cd在石花菜体内含量最高,为6.77mg/kg;Ba在海门冬体内含量最高,为127.02mg/kg。各藻类对各重金属元素的综合富集能力由强到弱依次为细江蓠拟刚毛藻水云海蕴鼠尾藻海门冬条浒苔小珊瑚肠浒苔蜈蚣藻礁膜大石花菜石花菜半盐生单歧藻团扇藻长石莼宽扁叉节藻穗状鱼浒苔喇叭菜海头红刚毛藻,显示出藻类对各重金属元素综合富集能力因藻而异。各元素被藻类富集的量从大到小依次为FeMnBaZnCrCuPbAsSeCdHg,显示出藻类对营养元素的富集要大于对毒性元素的富集。各藻类对各重金属的富集系数从几倍至几十万倍不等,最高是细江蓠对Fe的富集,其次为海蕴对Hg的富集,最差为刚毛藻对Se的富集,其富集系数分别为204875.29、13800.00、3.48。聚类分析结果显示细江篱的富集能力最强,是多种重金属污染海域修复的首选生态环境材料。但对重金属复合污染海域进行生物修复,应有针对性地选择数种富集能力较强的大型海藻组成修复生物群落。  相似文献   

10.
海藻乙醇提取物抗菌活性的研究   总被引:26,自引:2,他引:26  
分属绿藻门、红藻门、褐藻门的海藻样品于 2 0 0 0年 5月采于山东青岛和威海附近沿海 ,将海藻样品的乙醇提取物进行薄层层析分离和抗大肠杆菌、金黄色葡萄球菌活性测定。结果表明 ,抗大肠杆菌活性较强的海藻样品有 :鸭毛藻、酸藻、松节藻和孔石莼的水相 ;抗金黄色葡萄球菌活性较强的提取物有 :鸭毛藻、海黍子、松节藻、小粘膜藻的乙酸乙酯相以及酸藻的水相。从不同门类海藻的抗菌活性看 ,褐藻最强 ,红藻次之 ,而绿藻活性最弱。薄层层析分离得到 1 6个层析带具有抗金黄色葡萄球菌活性 ,1 1个层析带具有的抗大肠杆菌性。其中褐藻门的酸藻、海黍子的 3个层析带具有很强的抗金黄色葡萄球菌活性 ,酸藻的 2个层析带也能强烈地抑制大肠杆菌的生长。在同样的实验条件下 ,对常见抗生素进行的对照实验表明 ,这 5条层析带的活性比常见的抗生素高。  相似文献   

11.
1 Introduction R ed tide is a global m arine ecological calam ity.In recentyears, there has been an increase in frequen-cy, affected area and extentof injury of red tide out-breaks in the coastalw aters ofour country.A ccordingto China M arine D isaster B ulletin issued by the StateO ceanic A dm inistration of C hina, harm ful red tideshad occurred up to 119 tim es in 2003, w hich w as 40tim es m ore than thatin 2002. O w ing to the w orsenedcultural environm ent and increased nutrient en…  相似文献   

12.
利用共培养系统,研究了不同氮、磷营养盐浓度和光照强度下龙须菜Gracilaria lemanei for-mi:对赤潮异弯藻Heterosigma akashiwo和海洋原甲藻Prorocentrum micans生长的影响及龙须菜水提取物对2种赤潮藻生长的抑制效应,分析了龙须菜的抑藻机理.结果表明,在氮、磷浓度分别为...  相似文献   

13.
3种赤潮微藻对UV-B辐射处理的敏感性   总被引:4,自引:1,他引:3  
通过实验生态学的方法,研究了赤潮异弯藻(Heterosigma akashiwo)、亚历山大藻(Alexandrium tamarense)和中肋骨条藻(Skeletonema costatum)的生长对UV—B辐射增强的响应。结果表明,UV—B辐射处理对赤潮异弯藻的生长始终表现出抑制效应,而低剂量的UV—B辐射处理对亚历山大藻和中肋骨条藻的生长有刺激作用,高剂量的UV—B辐射处理对亚历山大藻和中肋骨条藻的生长仍然显示出抑制作用。UV—B辐射对赤潮异弯藻、亚历山大藻和中肋骨条藻的96h半抑制剂量分别为1.63、2.34和2.58J/m^2。  相似文献   

14.
不同氮源对微藻增殖的影响   总被引:12,自引:1,他引:11  
采用室内实验的方式研究了无机氮和有机氮对不同微藻增殖的影响情况。比较了不同氮源(氯化铵和尿素)对纤细角毛藻(Chaetoceros gracilis)、赤潮异弯藻(Heterosigma akashiwo Hada)、亚历山大藻(Alexandrium sp.)、隐藻(Chroomonas salina f.adolenscens)4种海洋微藻生长的不同影响。结果表明,有机氮尿素对海洋微藻增殖的影响比无机氮的复杂。由微藻最适生长的氮、磷浓度得到N:P值,讨论了该值对微藻增殖的影响,以及影响N:P值的可能情形。  相似文献   

15.
A two-month study was conducted to test the e?ects of macroalgae on the growth and survivorship of juvenile lined seahorse (Hippocampus erectus) in aquaria. Twenty-day old seahorses were cultured in the tanks with green alga Chaetomorpha linum, red alga Gracilaria tikvahiae, green plastic artiˉcial plant (stargrass), and black plastic net, respectively. Increases in wet mass and standard length for the seahorses were signiˉcantly higher in the tanks with macroalgae than those without macroalgae. The seahorse survivorships in the two macroalgal treatments were also signiˉcantly higher than those of the two treatments without macroalgae. In the treatments with macroalgae, the DO and pH were higher than those without marcoalgae. During the experiment, the color of seahorses was changed to certain extent. In the green background (the treatments with C. linum and stargrass), 24.1%{28.4% of black seahorses decreased over time; whereas in the black (plastic net) or brown (G. tikvahiae) background treatments, 14.1%{16.3% of yellow seahorses decreased over time, for matching that of the background. Furthermore, the survival rate of seahorse was correlation with DO and pH, and strong correlation with standard length, wet mass and CF. Polyculture with macroalgae, survival rates of seahorse were higher than without macroaglae.  相似文献   

16.
铅离子对海洋浮游植物生长影响的研究   总被引:4,自引:0,他引:4  
采用一次培养实验方法,研究了Pb^2 对8种常见海洋浮游植物生长的影响。在Logistic生长模型的基础上,结合Lorentz方程和GaussAmp方程,引入Pb^2 浓度项,建立新的方程来描述Pb^2 存在条件下海洋浮游植物的生长过程,并且通过对实验数据的非线性拟合,验证该方程是合理的。实验拟合结果表明,高浓度Pb^2 (c(Pb^2 )≥2500μg/L)对浮游植物的生长普遍有抑制作用,而较低浓度Pb^2 (c(Pb^2 )≤100μg/L)则易促进赤潮异弯藻(Heterosigma akashiuo Hada)、旋链角毛藻(Chaetoceros curvisetus Cleve)、三角褐指藻(Pheodactylum tricormutum Bohlin)、中肋骨条藻(Skeletonema costatum (Greville) Cleve)、青岛大扁藻(Platymonas helgolanidica Kylin var tsingtaoensis)、亚心型扁藻(Platymonas subcordiformis)的生长,它们最佳的促进生长浓度分别为:1991、2523、101、1488、627、509μg/L,但对于海洋原甲藻(Prorocentrum micans Ehrenberg)、裸甲藻(Gymnodinium sp.)的生长没有明显的影响。应用该方程在一定程度上不仅可以根据浮游植物的生长情况.推测相应海区的Pb^2 污染物浓度;而且也可以预测不同浓度Pb^2 条件下,相应海区的海洋浮游植物的生长情况。  相似文献   

17.
Allelopathic effects of several concentrations of fresh tissue,dry powder and dry tissue of three bloom-forming green macroalgae Ulva pertusa,Ulva linza and Enteromopha intestinalis on the red tide microalga Heterosigma akashiwo were evaluated in microcosms systems.The effects of macroalgae culture medium filtrate were investigated on H.akashiwo using initial or semi-continuous filtrate addition.Preliminary studies on the algicidal effects of one aqueous and four organic solvent extracts from the macroalgae on the microalga were carried out to confirm the existence of allelochemicals in the tissue of these macroalgae.The dry powder of U.pertusa was extracted with methanol,and the methanol extracts were partitioned to petroleum ether phase,ethyl acetate phase,butanol phase and distilled phase by liquid-liquid fractionation.The bioassays of the activity of every fraction were carried out on H.akashiwo.The resultant microcosms assay showed that the growth of H.akashiwo was strongly inhibited by using fresh tissues,dry powder or dry tissue of these three macroalgae,while aqueous and methanol extracts of both macroalgae had strong inhibitory effects on the growth of H.akashiwo,and the EC 50 values for methanol extract of U.pertusa,U.linza or E.intestinalis were 0.016,0.028×10-12 or 0.033×10-12,respectively.While the other three organic solvent extracts(acetone,ether and chloroform) had no apparent effect on its growth,this suggests that the allelochemicals from these three macroalgae had relatively high polarities.The activity of petroleum ether phase,ethyl acetate phase,butanol phase and distilled phase of U.pertusa methanol extract was carried out on H.akashiwo indicating that petroleum ether phase and ethyl acetate phase had stronger algicidal effect on H.akashiwo.The inhibition effect of the ethyl acetate phase was not as strong as that of petroleum ether phase,and effective concentration of petroleum ether phase was 17 mg/L for H.akashiwo.However,no significant algicidal effects were observed on the butanol phase and distilled water phase.These three macroalgae's culture medium filtrate exhibited no apparent growth inhibitory effect on the microalga under initial filtrate addition whereas the growth of H.akashiwo was significantly inhibited under semi-continuous filtrate addition,which suggests that continuous release of small quantities of rapidly degradable allelochemicals from the fresh tissue of both macroalgae was effective in inhibiting the growth of H.akashiwo.  相似文献   

18.
Effects of four nitrogen substrates on growth of several red tide species   总被引:1,自引:0,他引:1  
--Eight common red tide species including the Dinoflagellates Ale randrium tamarense,Prorocentrum minimum, Prorocentrum mican, Diatoms Skeletonema costatum , Cerataulina pelagica, Leptocylindrus minimus, a Raphidophyte Heterosigma akashiwo and a Chrysophyte Ivohrysis galbana were chosen to study the effect of four nitrogen substrates ( NO3 - N, NH4 - N, Urea - N,Mixed - N) on the growth of these algae. After two transfers, the results showed that at N concentration of 550μrnol, NO3 N was the best nitrogen source among these four nitrogen substrates; Urea - N and Mixed- N were also good for the growth of most algae, but not as good as NO3 - N for some species; NH4 - N inhibited the growth of all these species except H. akashiwo. At concentrations of 280 and 50 μmnol experiments on the growth of I. galbana, P. minimum and L. minimus in NH4 - N and NO3 - N substrates were also performed. The results showed that the growth rates of I. galbana were not significantly different from each other in NO3 - N and NH4 - N substrates both concentrations of 280 and 50 μmol. In concentration of 280μmol NH4 - N substrate, the growth rates of P.minimum and L. minimus were slower than in same concentration of NO3 - N; At concentration of 50μrmol , the growth rate of P. minimum in NH4 - N was not significantly different from that in NO3 - N, while the growth rate of L. minimus was only about 30% of that in NO3 - N substrate.The results indicated that each alga had its own preference in N-substrate and concentration, therefore,different nitrogen substrates may play a role in red tide formation.  相似文献   

19.
研究了从浙江海区分离的一株微小卡罗藻(Karlodinium micrum)对鲫鱼动脉血的溶血性,以典型鱼毒性的赤潮异弯藻作为比较,分别就细胞提取液、去藻上清液和细胞悬浮液的溶血性进行了研究。结果发现:同样处理条件下,以1%的Triton X-100作为溶血度100%的指标,微小卡罗藻细胞提取液的溶血度为70%,赤潮异弯藻细胞提取液的溶血度为32%。微小卡罗藻去藻上清液的溶血度为33%,赤潮异弯藻去藻上清液的溶血度大于100%溶血的TritonX-100组。两种藻的细胞悬浮液溶血度接近0。去藻上清液和细胞提取液具有显著浓度相关的溶血特征。在同样藻密度时,去藻上清液的溶血度大于细胞提取液的溶血度。由于微小卡罗藻在形态上容易与无毒的河口裸甲藻(Gyrodinium estuariales)混淆,因此,对于该藻的环境危害应当加以重视。对于微小卡罗藻具有溶血性的报道尚属首次。  相似文献   

20.
在添加浒苔和紫菜干体的条件下,通过测定藻细胞数量,观察藻细胞形态,分析海藻干体对米氏凯伦藻、中肋骨条藻和塔玛亚历山大藻的半抑制和完全抑制浓度以及半抑制和完全抑制浓度有效作用的起始藻细胞数量范围,研究了浒苔和紫菜干体对3种赤潮微藻生长的影响.在此基础上,设定海藻干体的添加量为其完全抑制浓度,通过监测微藻培养液中总氮(硝酸态氮、氨态氮和亚硝酸态氮)和总磷等变化,分析海藻干体添加对培养液营养化程度的影响.结果表明:(1)当浒苔和紫菜干体浓度分别大于0.8 g/L 和1.0 g/L 时,海藻干体能显著抑制3种赤潮微藻的生长,它们对赤潮微藻的生长抑制率超过60%(第12 d).(2)浒苔和紫菜干体对3种实验微藻的半抑制浓度(IC50)接近,在0.71~1.22 g/L 之间;但完全抑制浓度(IC10)差别较大,浒苔干体对3种赤潮微藻的完全抑制浓度在2.4~2.8 g/L 范围,其数值约为紫菜干体对它们的完全抑制浓度的2倍.(3)3种赤潮微藻起始藻细胞数量不超过40伊104·mL-1(对于米氏凯伦藻和中肋骨条藻来说,起始藻细胞数量可增大至60伊104·mL-1),海藻干体对3种实验微藻的半抑制浓度和完全抑制浓度均有效.(4)添加海藻干体的实验组培养液中总氮和总磷含量均明显高于对照培养液中总氮和总磷含量.其中,实验组培养液中总氮浓度为382滋g/L,总磷浓度明显低于100滋g/L.综上所述,浒苔和紫菜干体具有强烈的抑藻作用,且不会造成培养液营养加剧现象,可尝试应用于赤潮微藻的控制与治理中.  相似文献   

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