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1.
根据日立835—50型氨基酸自动分析仪生理体液分析法，以及同型胱氨酸的生理特性，建立测定同型胱氨酸的150min短程序法。经过精密度和准确度试验，重视性好，变异系数小。当同型胱氨酸浓度为4×10－5mol／L时，峰位置和峰面积的变异系数（CV值）分别为0．20％和1．98％（n＝10），明显低于规定技术指标（1％和2．5％）。对1、2、4（×10－4mol／L）浓度的同型胱氨酸加标回收试验，回收率为叶．33～106．67％（n＝9）。本法与原法同时测定大鼠及人体血浆的同型胱氨酸的含量结果基本一致。表明本方法准确、可靠、快速、简便，适用于高脂血症、动脉粥样硬化以及血栓等血浆中同型胱氨酸的测定。本方法样品处理采用任其与空气接触，使血浆中同半型胱氨酸氧化为同型胱氨酸，测定其总同型胱氨酸含量。 相似文献
2.
类肝素新药“916”对高脂血症大鼠血清脂蛋白及过氧化脂质产物含量的影响(英文) 总被引：1，自引：0，他引：1
初步探讨了类肝素海洋新药“916”对高脂血症大鼠血清脂蛋白胆固醇及过氧化脂质的影响。结果表明,“916”能够明显抑制高脂饲料诱发的血清总但固醇及低密度脂蛋白胆固醇的升高;显著降低高脂血症大鼠血清过氧化脂质的含量。 相似文献
3.
从合浦珠母贝Ｐｉｎｃｔａｄａ ｆｕｃａｔａ （Ｇｏｕｌｄ）和刁海龙Ｓｏｌｅｎｏｇｎａｔｈｍｓ ｈａｒｄｗｉｃｈｉ（Ｇｒａｙ）中提取而制成的龙珠液，给大鼠灌服（０．７５、１．５０、２．２５ｇ．ｋｇ＾－１）可显著抑制由高脂食物诱发的高脂血症；给小鼠灌服（２．２５、３．７５ｇ．ｋｇ＾－１）可显著增强其对异物颗粒的廓清能力，增高淋平细胞化的转化率，增加前列腺贮精囊的重量。 相似文献
4.
岩藻聚糖硫酸酯低聚糖降压作用的初步研究 总被引：8，自引：0，他引：8
采用两肾—夹型建立肾血管性高血压大鼠模型。探讨岩藻聚糖硫酸酯低聚糖对肾血管性高血压大鼠的血压、呼吸及血浆中血管紧张素Ⅱ(Ang Ⅱ)含量的影响。用GY-6088型多道生理参数分析记录仪和张力传感器测定、记录血压和呼吸；用均相竞争法测定Ang Ⅱ的含量。实验表明，12．5，25，50mg／kg的岩藻聚糖硫酸酯低聚糖均能显著地降低肾血管性高血压大鼠的动脉血压。其中，高剂量(50mg／kg)的降压效果与14mg／kg的卡托普利相当；给药大鼠的呼吸频率随血压的变化呈正相关变化；12．5，25，50mg／kg剂量的岩藻聚糖硫酸酯低聚糖均能降低高血压大鼠血浆中Ang Ⅱ的含量。其中，高剂量(25，50mg／kg)组的Ang Ⅱ水平同卡托普利组(14mg／kg)相当。岩藻聚糖硫酸酯低聚糖对肾血管性高血压大鼠有显著的降压作用，抑制AngⅡ生成可能是其降压机制之一。 相似文献
5.
研究墨西哥刺参(Isostichopus fuscus)对大鼠脂质代谢的调节作用及其机理.采用灌胃高脂乳剂方法建立实验性高胆固醇血症大鼠模型,检测模型大鼠血清总固醇(Total cholesterol, TC)、甘油三酯(Triglyceride, TG)、低密度脂蛋白胆固醇(Low density lipoprotein cholesterol, LDL-C)、高密度脂蛋白胆固醇(High density lipoprotein cholesterol, HDL-C)含量;肝脏TC、TG含量和肉毒碱棕榈酰转移酶-1(Carnitine palmitoyl transterase-1, CPT-1)、过氧化物酶体β-氧化酶系(peroxisomal β-oxidation enzymes)、磷脂酸磷酸酶(Phosphatidic acid phophyhydrolase, PAP)等脂质代谢相关酶活力;粪便总脂质、TC和胆汁酸含量.实验结果显示,墨西哥刺参能显著降低高胆固醇血症大鼠血清TC(P<0.01)、LDL-C(P<0.05)含量及LDL-C/HDL-C(P<0.05);显著降低肝脏TC(P<0.01)、TG(P<0.01)含量,提高肝脏CPT-1(P<0.01)、peroxisomal β-oxidation enzymes(P<0.05)和PAP(P<0.05)活力;显著提高粪便中总脂质(P<0.01)、TC(P<0.01)和胆汁酸(P<0.01)含量.提示墨西哥刺参能有效调节高胆固醇血症大鼠脂质代谢,其主要作用机理是促进脂质在体内的分解代谢,同时抑制消化道内脂质的吸收. 相似文献
6.
石油污染对非洲鲫鱼血清电解质和胆固醇的影响 总被引：4，自引：0，他引：4
本文通过分析非洲鲫鱼血清电解质和胆固醇含量的变化，研究石油对鱼类的致毒效应，实验结果表明：（１）石油对血清电解质没有影响；（２）石油能降低血清胆固醇含量；（３）石油对非洲鲫血清胆固醇产生作用的浓度阈值小于０．２４ｍｇ／Ｌ，认为血清胆固醇可以作为石油污染的监测指标。 相似文献
7.
目的：探究煜鹤堂蝮蛇酒对血糖血脂和血压的调节作用。方法：将检疫合格的SD大鼠、自发性高血压（SHR）大鼠随机分为正常对照组、模型对照组、溶媒对照组及煜鹤堂蝮蛇酒高、中、低剂量组（用药剂量分别相当于成人推荐食用量的20、10和5倍）。SD大鼠除正常对照组外，其余各组大鼠给予定量高脂高糖饲料喂养，同时按10ml/kg给予受试物或溶媒，连续4周，末次给样后检测血糖（GLU）以及血清中总胆固醇（TC）、三酰甘油（TG）、高密度脂蛋白（LDL-C）及低密度脂蛋白（HDL-C）水平。SHR大鼠按10ml/kg灌胃给予受试物4周，每周检测各组大鼠体质量和血压。结果：煜鹤堂蝮蛇酒各剂量均可显著降低高糖高脂模型大鼠GLU和TC水平（P<0.01），高、中剂量可显著降低模型大鼠TG及LDL-C水平（P<0.01或P<0.05）；煜鹤堂蝮蛇酒高剂量可明显降低SHR大鼠收缩压（P<0.05），对实验动物体质量无明显影响。结论：煜鹤堂蝮蛇酒具有明显的降糖调脂和降压作用。 相似文献
8.
采用肾血管性高血压大鼠模型（两肾一夹型）观察海洋硫酸多糖DPS对肾血管性高血压大鼠血清中一氧化氮(NO)和血浆中血管紧张素II（AngII）及内皮素-1(ET-1)含量的影响。DPS在肾血管性高血压大鼠造模第二天起分别以12.50,25.00，50.00mg/kg口服预防给药五周，每日给药一次。于给药前、给药后第三周和第六周分别测定动脉血压和心率。实验结束前，从每只大鼠取血6mL，用试剂盒测定血清中NO的含量；用放射免疫法测定血浆中AngII和ET-1的含量。血管紧张素转化酶抑制剂卡托普利（14mg/kg）作为本实验阳性对照药。结果：DPS口服预防给药五周，可显著增加血清中NO的含量和降低血浆中ET-1的含量，且呈剂量依赖性；DPS亦能降低血浆中AngII的含量，但未见剂量依赖性。结论：海洋硫酸多糖DPS对肾血管性高血压大鼠的降压作用机制可能与其促进体内NO生成或释放、降低AngII和ET-1的含量有关。 相似文献
9.
本研究采用添加10%不同水产品蛋白的标准饲料或高胆固醇饲料喂食小鼠,研究高胆固醇水产品对小鼠脂质代谢的影响。通过测定小鼠血清总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白(HDL-c)的浓度以及肝脏TC、TG和磷脂(PL)的含量,同时称量小鼠体质量、肝脏、肾脏重量以及肾周脂肪和精巢上部脂肪重量,以评价不同蛋白质对小鼠成长的影响,并收集了高胆固醇血症模型小鼠的排泄物并测定其总脂质和中性固醇的排泄量。结果显示,水产品作为优质蛋白质能明显降低正常小鼠和高胆固醇血症模型小鼠的血清总脂水平(P<0.05)以及动脉粥样硬化指数(P<0.05),不同水产品对小鼠肝脂水平及高胆固醇血症小鼠排泄物中总脂质和中性固醇的排泄量的影响因水产品种类而不同。提示本研究中的高胆固醇水产品对由高胆固醇血症引发的动脉粥样硬化不具有危害性。 相似文献
10.
目的：研究金花茶、银杏叶单用及配伍对高脂饲料诱导的高脂模型大鼠体质量、脂肪指数、血脂水平以及肝脏的影响。方法：将40只雄性SD大鼠随机分为5组，即空白组、模型组、金花茶组（给药剂量为0.053 g/kg）、银杏叶组（给药剂量为0.013 g/kg）、银杏叶+金花茶组（给药剂量为0.066 g/kg），空白组予以普通饲料喂养，其余各组均予以高脂饲料喂养40 d。饲养10 d后开始药物干预，灌胃体积均为1.0 ml /100 g，空白组与模型组给予同体积纯化水，给药时间为30 d。考察不同时期大鼠体质量与血脂水平的变化，检测大鼠脂肪指数、肝脏脂肪变性情况。结果：灌胃给予受试样品30 d，金花茶、银杏叶、金花茶+银杏叶均能控制高脂模型大鼠的体质量与脂肪指数，降低血清三酰甘油（TG）、总胆固醇（TC）、低密度脂蛋白胆固醇（LDL-C）水平，提高高密度脂蛋白胆固醇（HDL-C）水平，还可不同程度地改善大鼠肝脏的脂肪变性，其中以银杏叶+金花茶组效果最佳。结论：金花茶与银杏叶单用及合用均有降脂和改善、预防脂肪肝的作用，且综合各项指标的检测结果，二者配合使用的降脂功效最优。 相似文献
11.
Arne Schnack-Friedrichsen Angela M. Davis Jim D. Bennell Dei G. Huws 《Marine Georesources & Geotechnology》2001,19(4):221-243
The interdependence between the seismo-acoustic properties of a marine sediment and its geotechnical/physical parameters has been known for many years, and it has been postulated that this should allow the extraction of geotechnical information from seismic data. Though in the literature many correlations have been published for the surficial layer, there is a lack of information for greater sediment depths. In this article, a desktop study on a synthetic seafloor model illustrates how the application of published near-surface prediction equations to subsurface sediments (up to several tens of meters burial depth) can lead to spurious predictions. To test this further, acoustic and geotechnical properties were measured on a number of sediment core samples, some of which were subjected to loading in acoustically-equipped consolidation cells (oedometers) to simulate greater burial depth conditions. For low effective pressures (representing small burial depths extending to around 10 meters subsurface), the general applicability of established relationships was confirmed: the prediction of porosity, bulk density, and mean grain size from acoustic velocity and impedance appears generally possible for the investigated sedimentary environments. As effective pressure increases through, the observed relationships deviate more and more from the established ones for the near-surface area. For the samples tested in this study, in some instances increasing pressure even resulted in decreasing velocities. There are several possible explanations for this abnormal behavior, including the presence of gas, overconsolidation, or bimodal grain size distribution. The results indicate that an appropriate depth correction must be introduced into the published prediction equations in order to obtain reliable estimates of physical sediment properties for greater subsurface depths. 相似文献
12.
Derek V. Ellis 《Marine Georesources & Geotechnology》2001,19(1):51-63
This article reviews information recently available from existing marine and coastal mining for responses to environmental issues affecting marine mining at different depths. It is particularly but not exclusively concerned with those issues affecting seabed biodiversity impact and recovery. Much information has been gathered in the past 10 years from shallow mining operations for construction aggregate, diamonds, and gold, from coastal mines discharging tailings to shallow and deep water, and from experimental deep mining tests. The responses to issues identified are summarized in a series of eight tables intended to facilitate site-specific consideration. Since impacts can spread widely in the surface mixing layer SML, and can affect the biologically productive euphotic zone, the main issues considered arise from the depth of mining relative to the SML of the sea. Where mining is below the SML, the issue is whether it is environmentally better to bring the extraction products to the surface vessel for processing (and waste discharge), or to process the extraction products as much as possible on the seabed. Responses to the issues need to be sitespecific, and dependent on adequate preoperational environmental impact and recovery prediction. For deep tailings disposal from a surface vessel, there are four important environmental unknowns: (1) the possible growth of "marine snow" (bacterial flocs) utilizing the enormous quantities of fine tailings particles (hundreds or thousands of metric tons per day) as nuclei for growth, (2) the possibility that local keystone plankton and nekton species may migrate diurnally down to and beyond the depth of deep discharge and hence be subjected to tailings impact at depth, (3) the burrow-up capability of deep benthos and their ability to survive high rates of tailings deposition, and (4) the pattern and rate of dispersion of a tailings density current through the deep water column from discharge point to seabed. Actions to obtain relevant information in general and site-specifically are suggested. 相似文献
13.
G. Parthiban 《Marine Georesources & Geotechnology》2000,18(3):223-235
Particle fluxes were measured 7 m above the sea bottom during the predisturbance, disturbance, and postdisturbance periods by using time series sediment traps attached to seven deep-sea moorings deployed in the INDEX experiment site in the Central Indian Basin. The predisturbance particle fluxes varied between 22.3 to 55.1 mg m -2 day -1 . Increased and variable particle fluxes were recorded by the sediment traps during the disturbance period. The increase observed was 0.5 to 4 times more than the background predisturbance fluxes. The increases in particle fluxes (~4 times) recorded by the sediment trap located in the southwestern direction (DMS-1) were the greatest, which could be the result of preferential movement of resuspended particles generated during the deep-sea benthic disturbance along the general current direction prevailing in this area during the experimental period. Also, the traps located closer to the disturbance area recorded greater fluxes than did the traps far away, across the Deep Sea Sediment Resuspension System path. This variability in recorded particle fluxes by the traps around the disturbance area clearly indicates that physical characteristics such as grain size and density of the resuspended particles produced during the disturbance had an important effect on particle movement. The postdisturbance measurements during ~5 days showed a reduction in particle fluxes of ~50%, indicating rapid particle settlement. 相似文献
14.
海上大直径钢管桩打桩过程中,桩周土体受到强烈扰动而发生强度弱化,掌握桩周土体强度弱化规律对于准确预测打桩过程、保证工程安全具有重要意义。为研究土体强度弱化规律,开展了环剪试验模拟打桩对桩周土体的扰动,测试土体强度随剪切速率的变化规律,建立了描述土体强度弱化规律的拟合公式,引入到打桩分析软件中。研究结果表明：土体的强度折减程度不仅与土体本身的性质有关还受到土体的埋深和剪切速率的影响,埋深越深土体强度折减程度越低,剪切速率越高土体强度折减越高,在打桩分析中可采用这里推荐的线性折减方法来模拟不同深度处土体强度的折减规律。 相似文献
15.
The advanced piston corer (APC) has been used by the Ocean Drilling Program since 1985 for recovering soft sediments from the ocean floor. The pullout force measured on extracting the core barrel from the sediment is shown to correlate with the average shear strength of the sediment core measured in the ship's laboratory. A simple rule of thumb is derived relating the shear strength of the sediment to the pullout force. Multiple APC holes at individual sites allow the consistency of the pullout measurements to be assessed. The effects of different operational procedures during APC coring are also explored. Although generally applicable, the correlation between pullout force and laboratory measurements of shear strength breaks down for some APC holes, possibly because of the disturbance of some sediment types during the APC coring process. A better understanding of the physical process of APC coring, and its effect on the properties of the sediment both inside and immediately outside the core barrel, would indicate what confidence can be put on the measurement of pullout force as a way of evaluating the in situ shear strength of deep sea sediments. 相似文献
16.
Shigehisa Nakamura 《Marine Geodesy》2000,23(1):55-61
Specific properties of the interannual sea level variations and annual tides in the Northwestern Pacific were studied. Several tide stations were monitored. The monthly mean sea level for the year of 1995 was analyzed at each tide station. A seismic event in 1995, some tectonic activity around the subject area, and the Kuroshio (the oceanic western boundary current) may possibly contaminate results which would have occurred from the astronomical annual tide alone. 相似文献
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《海洋学报(英文版)》2014,(11):161-F0003
<正>Acta Oceanologica Sinica(AOS)is a comprehensive academic journal edited by the Editorial Committee of Acta Oceanologica Sinica and is designed to provide a forum for important research papers of the marine scientific community which reflect the information on a worldwide basis.The journal publishes scholarly papers on marine science and technology,including physics,chemistry,biology, 相似文献