共查询到20条相似文献，搜索用时 46 毫秒
1.
通过Wright染色对长鳍裸颊鲷(Lethrinus erythropterus)的头肾、体肾、脾脏、肝脏等组织涂片、印片的观察,发现其头肾、体肾、脾脏是其主要的造血器官。红细胞、粒细胞、淋巴细胞和单核细胞主要在头肾和体肾中发生,其次是脾脏。肝脏中无原始型血细胞,可能不是其造血器官。血细胞的发生大致经过三个阶段,即原始阶段、幼稚阶段、成熟阶段。本文着重描述了长鳍裸颊鲷的红细胞、嗜中性粒细胞、嗜酸性粒细胞、嗜碱性粒细胞、淋巴细胞和单核细胞发生过程中各阶段细胞的形态特征。嗜碱性粒细胞的发育也可以在头肾中被发现。 相似文献
2.
3.
报道了青弹涂鱼 ( Scartelaos virids ) 外周血细胞的显微结构,血涂片经过Wright液染色,可鉴定出红细胞、单核细胞、淋巴细胞、血栓细胞、嗜中性粒细胞和嗜碱性粒细胞,未发现嗜酸性粒细胞.红细胞数量多,圆形,具圆核;血栓细胞体积最小,呈三五个聚集分布;嗜碱性粒细胞体积最大.淋巴细胞大小有三种:大淋巴细胞、中淋巴细胞和小淋巴细胞.在数量上,血栓细胞最多,淋巴细胞次之,单核细胞,嗜中性粒细胞和嗜碱性粒细胞则较少.此外,还见到红细胞、单核细胞可在外周血液中通过直接分裂产生,这说明红血细胞及白血细胞的部分种类也可在外周血直接分裂. 相似文献
4.
通过Wright-Giemsa染色对斜带髭鲷[Hapalogenys nitens (Richardson)]的头肾、体肾、脾脏、肝脏四组织涂片、印片的观察,发现其头肾、体肾、脾脏是其主要的造血器官。头肾能发育生成各类型血细胞,体肾能生成红细胞和粒细胞,脾脏能生成淋巴细胞和红细胞。肝脏中未发现幼稚型血细胞。血细胞发生大致经过原始、幼稚和成熟三个阶段。细胞化学染色显示:外周血细胞中,除淋巴细胞外,均有糖原;嗜中性粒细胞含有ACP、ALP、NACE、αNAE和POX;嗜酸性粒细胞含有ACP、NACE和POX;单核细胞含有ACP、αNAE和POX;小淋巴细胞有ACP。研究发现斜带髭鲷是一种抗病能力较强的鱼类。血细胞组织化学染色可作为斜带髭鲷疾病诊断的辅助手段。 相似文献
5.
《热带海洋学报》2017,(5)
为查明杂交石斑鱼Epinephelus fuscoguttatus♀×Epinephelus lanceolatus♂血细胞形态分类及血细胞发生特点,文章通过血液和组织涂(印)片、细胞染色和显微观察,研究了体重263.18±12.31g的杂交石斑鱼外周血液白细胞的分类组成,以及头肾、脾脏、体肾和肝脏等4种组织中各类血细胞的发生情况,并将结果与其母本进行比较。结果表明,杂交石斑鱼外周血液白细胞由淋巴细胞[(55.46±12.38)%]、血栓细胞[(21.11±5.38)%]、嗜中性粒细胞[(19.44±12.02)%]、单核细胞[(1.66±2.13)%]和嗜酸性粒细胞[(2.33±1.91)%]组成;4种组织印迹片中,头肾中未成熟的细胞总数比例最高[(16.12±0.30)%]],体肾次之[(7.90±0.35)%],脾脏[(2.56±0.21)%]和肝脏[(0.97±0.18)%]未成熟细胞比例较低;由此推断杂交石斑鱼主要造血组织是头肾,其次是体肾。在杂交石斑鱼4种组织中均观察到浆细胞,在头肾、体肾和脾脏印迹片中观察到嗜碱性粒细胞和巨噬细胞,在体肾印迹片中观察到巨大原单核细胞。杂交石斑鱼与其母本相比,外周血液白细胞中淋巴细胞比例接近(+2.16%),血栓细胞较少(-14.58%),嗜中性粒细胞(+9.10%)、嗜酸性粒细胞(+2.18%)和单核细胞(+1.38%)较多;主要造血组织都是头肾,但淋巴细胞的主要发生组织不同(分别是体肾和头肾),次要造血组织也不同(分别是体肾和脾脏);此外杂交石斑鱼各组织中未成熟细胞比例明显高于母本,显示出活跃的造血活动。本研究结果能为杂交石斑鱼的血液生理学、病理学研究及石斑鱼杂交选育提供基础资料。 相似文献
6.
红鳍笛鲷外周血细胞的显微结构观察 总被引：3，自引：0，他引：3
报道了红鳍笛鲷(Lutjanus erythopterus)外周血细胞的显微结构,血涂片经过Wright染色液染色,可区分出:红血细胞、血栓细胞、嗜中性粒细胞、单核细胞、淋巴细胞等5种血细胞,无嗜碱性粒细胞、嗜酸性粒细胞。白血细胞中:血栓细胞体积最小,单核细胞体积最大;血栓细胞数目最多,嗜中性粒细胞和单核细胞数目最少;淋巴细胞有大中小3种类型:大淋巴细胞、中淋巴细胞和小淋巴细胞。此外,在外周血液中还观察到少量幼稚的红细胞,偶而可见正在分裂的红细胞,提示红细胞也可在外周血直接分裂。 相似文献
7.
报道了青石斑外周血细胞的显微结构,血涂片经过wright染色液染色,可区分出:红血细胞、血栓细胞、嗜中性粒细胞、单核细胞、淋巴细胞等5种血细胞,未发现嗜碱性粒细胞、嗜酸性粒细胞。白血细胞中,血栓细胞体积最小,单核细胞体积最大;在数量上,血栓细胞最多,淋巴细胞次之,单核细胞和嗜中性粒细胞则较少。青石斑的淋巴细胞可分为以下三种类型:大淋巴细胞、中淋巴细胞和小淋巴细胞。此外,在外周血液中还观察到少量未成熟的红血细胞和嗜中性粒细胞,幼稚型、成熟型和功能衰退型以及三者之间过渡类型的嗜中性白细胞皆可见到。偶尔可见正在分裂的红血细胞及单核细胞,提示红血细胞及白血细胞的部分种类也可在外周血直接分裂。 相似文献
8.
花尾胡椒鲷血细胞在光镜和扫描电镜下的结构 总被引：3，自引：0，他引：3
以花尾胡椒鲷血细胞进行光镜及扫描电镜观察。结果在血涂片中可分辨出红细胞、淋巴细胞、单核细胞、嗜酸性粒细胞、嗜中性粒细胞与血栓细胞,没有发现嗜碱性粒细胞。白细胞中,以淋巴细胞的比例最高,嗜酸性粒细胞数量最少。在扫描电镜下,红细胞椭圆形,表面光滑,无突起：而各种白细胞为圆形,细胞表面不平,具有各种突起,提示白细胞具有活跃的变形运动和吞噬能力。还见到红细胞、血栓细胞可在外周血液中通过直接分裂产生。 相似文献
9.
美国红鱼海上网箱养殖技术研究 总被引：3，自引：0，他引：3
美国红鱼属鲈形目,石首鱼科,拟石首鱼属,学名为Ｓｃｉａｅｎｏｐｓｏｃｅｌｌａｔｕｓ,中文直译名为眼斑拟石首鱼,在台湾等地又称红鱼、红姑鱼、斑尾鲈、黑斑红鲈等。体型与鮸鱼、大黄鱼近似,体色微红,尾鳍基部通常有１～４个黑色圆斑。美国红鱼原产墨西哥湾及美国西南部沿岸,是美国重要的商业养殖鱼类。１９９１年国家海洋局第一海洋研究所从美国引进该鱼的仔鱼进行入工养殖,对其在我国的生态、生长特性、养殖技术做了初步研究,并于１９９５年成功地培育出了第一代美国红鱼鱼苗［１～５］。美国红鱼具有生长快、适温广,能在海水… 相似文献
10.
采用组织学方法,获得患肾肿大症美国红鱼的心、脾、肾、肝、肠和鳃6种组织,利用组织切片以及超薄切片电镜技术对患病组织的病理变化进行了研究分析,探讨该病的致病机制。患病红鱼解剖后主要症状为:体表溃烂,部分病鱼鳍条上有肿块;病鱼肾脏显著肿大,体积甚至达到正常肾脏的1()倍;肝脏、脾脏及胆囊等也有肿大现象;有些病鱼脏器上有白色结节。组织病理显示:美国红鱼肾肿大症的主要表现为肾脏有典型的肉芽肿,大量炎性细胞浸润,肾小管管壁破裂、坏死;肝脏中肝实质有充血现象,实质细胞呈空泡变性;脾脏充血,现大量病变的红细胞,有脂肪样变。超徽病理显示:肾近曲小管上皮细胞凋亡现象严重,肾小管内刷状缘排列稀疏;肝细胞坏死严重,肝细胞界限模糊不清;脾脏组织脾窦扩张,有大量凋亡红细胞和淋巴细胞。实验结果表明:美国红鱼肾肿大症的组织细胞结构发生明显的病理变化,肾脏和肝脏等主要组织器官受到严重损伤病变,使鱼体不能进行正常的生命活动,最后导致死亡。 相似文献
11.
Arne Schnack-Friedrichsen Angela M. Davis Jim D. Bennell Dei G. Huws 《Marine Georesources & Geotechnology》2001,19(4):221-243
The interdependence between the seismo-acoustic properties of a marine sediment and its geotechnical/physical parameters has been known for many years, and it has been postulated that this should allow the extraction of geotechnical information from seismic data. Though in the literature many correlations have been published for the surficial layer, there is a lack of information for greater sediment depths. In this article, a desktop study on a synthetic seafloor model illustrates how the application of published near-surface prediction equations to subsurface sediments (up to several tens of meters burial depth) can lead to spurious predictions. To test this further, acoustic and geotechnical properties were measured on a number of sediment core samples, some of which were subjected to loading in acoustically-equipped consolidation cells (oedometers) to simulate greater burial depth conditions. For low effective pressures (representing small burial depths extending to around 10 meters subsurface), the general applicability of established relationships was confirmed: the prediction of porosity, bulk density, and mean grain size from acoustic velocity and impedance appears generally possible for the investigated sedimentary environments. As effective pressure increases through, the observed relationships deviate more and more from the established ones for the near-surface area. For the samples tested in this study, in some instances increasing pressure even resulted in decreasing velocities. There are several possible explanations for this abnormal behavior, including the presence of gas, overconsolidation, or bimodal grain size distribution. The results indicate that an appropriate depth correction must be introduced into the published prediction equations in order to obtain reliable estimates of physical sediment properties for greater subsurface depths. 相似文献
12.
Shigehisa Nakamura 《Marine Geodesy》2000,23(1):55-61
Specific properties of the interannual sea level variations and annual tides in the Northwestern Pacific were studied. Several tide stations were monitored. The monthly mean sea level for the year of 1995 was analyzed at each tide station. A seismic event in 1995, some tectonic activity around the subject area, and the Kuroshio (the oceanic western boundary current) may possibly contaminate results which would have occurred from the astronomical annual tide alone. 相似文献
13.
The effect of different fixation and storage protocols on the flow cytometric (FCM) simultaneous analysis of bacterioplankton and phytoplankton in coastal seawater samples (Mediterranean coastal lagoons) was investigated. FCM measurements (cell number, fluorescence and scatter characteristics) were obtained through DAPI staining. Three fixatives [glutaraldehyde (GA), formaldehyde (FA) and paraformaldehyde (PFA)] and two storage (3 months duration) methods (5 °C and −196 °C) were tested. Two dominant populations were detected in studied samples: bacteria and eukaryotic picophytoplankton. Adding fixatives (2% final concentration) appears necessary to obtain FCM exhaustive counts of all the bacteria and phytoplanktonic cells. This was related to the permeation effect of fixatives which allowed a better DAPI staining of the cells. Maximum fluorescence, i.e. optimal staining of the cells was obtained with FA or PFA, and significant lower fluorescences with GA. Fixed samples stored at 5 °C induced rapid cell loss. Only storage in liquid nitrogen of samples fixed with FA or PFA, allows mid-term (≥4 months) preservation of bacteria or picophytoplankton cell numbers, and limited evolution of DAPI-induced fluorescence and scatter characteristics. 相似文献
14.
15.
《海洋学报(英文版)》2014,(9):F0003-F0003
<正>Acta Oceanologica Sinica(AOS)is a comprehensive academic journal edited by the Editorial Committee of Acta Oceanologica Sinica and is designed to provide a forum for important research papers of the marine scientific community which reflect the information on a worldwide basis.The journal publishes scholarly papers on marine science and technology,including physics,chemistry,biology, 相似文献
16.
The relationship between the flux of exotic benthic foraminiferal tests (i.e. tests which are supplied from open-sea sources alone) in a tidal inlet and that of bulk sediment was analysed, which can be expressed as two first-order linear equations. According to this relationship, in order to determine net sediment transport directions in the entrance, the test concentration in surficial sediments of the tidal basin can be compared against a ‘ critical level ’. The critical level is determined for the conditions that no net transport of bulk sediment is present within the entrance. If the observed concentration (averaged over the tidal basin) is higher than the simulated critical level, then the net sediment transport is directed to landward. This method is applied to the analysis of net sand transport at Christchurch Harbour, a tidal inlet system located in southern England. In this investigation, concentrations of exotic foraminiferal tests in the surficial sediments of the tidal basin and ebb tidal delta area were obtained from the analysis of sea-bed sediment samples. A series of probable critical levels were calculated based upon the data sets with regard to: (1) sediment discharge from the rivers; (2) magnitude of sediment discharge within the entrance during the ebb; (3) the test concentration outside the harbour; (4) the thickness of the moving layer; and (5) two parameters associated with dispersive processes. The results show that the concentration in the tidal basin sediment is higher than a number of simulated critical concentrations for representative cases. Consequently, the high level of the concentration of exotic benthic foraminiferal tests within the harbour should be explained as a result of landward net transport of sands within the entrance. 相似文献
17.
18.
The behaviour of an axially pre-loaded cylindrical member of an offshore structure, hit by a supply ship, has been investigated. The effects of axial pre-loading on the dynamic properties of members, the extent of damage and propagation of dynamic instability in the tubular members have also been investigated. Numerical models have been validated using available experimental data from the literature although these are mostly static with no pre-loading. Axial pre-loading was found to change the dynamic characteristics of some cylindrical members quite dramatically while it had no significant effect on others. The study examined whether there was any change in the behaviour between local and global modes when the loading was dynamic rather than static. The effect of damping on the dynamic instability of axially pre-loaded tubes under lateral dynamic loads was also studied. It was observed that for some geometries the quasi-static response can be used to define the boundary between bounded and unbounded dynamic responses. The main contribution of this study, compared with previous investigations reported in the literature, is that both pre-loading and dynamic effects have been included. 相似文献
19.
Rahul Sharma 《Marine Georesources & Geotechnology》2000,18(3):237-250
Observations on seafloor features from subbottom profiling and seabed photography data show that the undisturbed and natural conditions before the benthic disturbance undergo a substantial change after operation of the disturber. The effects can be seen in the form of deep trenches and grooves, formed by the action of the sleds and the pumping of the sediment along its path, as well as sediment piles on either side of the track. Biological traces such as fecal coils, casts, tubes, burrows, and trails get obliterated in the areas of disturbance and resedimentation. The smooth, uniform nature of the seafloor observed in predisturbance phase shows microtopographic changes in and around the disturbed area as a result of sediment excavation and resettlement. Vertical mixing as well as lateral transport of sediment alter the geological, biological, physical, and chemical conditions on the seafloor, which need to be monitored over time to assess the process and the time taken for restoration of environmental conditions. 相似文献
20.
Vance P. Vicente 《Marine Ecology》1989,10(2):179-191
Abstract. Long-term changes in the distributional patterns of commercial sponges ( Spongia spp. and Hippospongia spp.) within the West Indian Region indicates that: 1) commercial sponges had a widespread distribution throughout the whole West Indian Region and were ubiquitous in very shallow water until about the first half of the present century; 2) they were fished commercially not only in the traditional northern Caribbean sites (Florida, Gulf of Mexico, Bahamas) but also in the Greater ( e. g. , Hispaniola, Jamaica) and Lesser Antilles; and 3) they became extinct throughout most of the Lesser Antilles ( e. g. , Puerto Rico, Vieques, St. Thomas) sometime during the first half of this century. Mortalities of spongiids within the Antilles were found to differ from other marine mortalities in that: 1) species disappeared from a large region; 2) species vanished from different habitats and depths; and 3) natural populations never recovered. Species richness patterns suggest that commercial sponge genera ( Spongia and Hippospongia ) evolved under slightly cooler elimatic conditions than those found at present, and that these extinctions occurred as a direct or indirect effect of positive thermal anomalies in sea surface and atmospheric temperatures between 1900–1950. The concept that species diversity is stable on a regional scale is questioned. 相似文献