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1.
An experiment on the phosphorus requirement and the proper Ca/P ratio in the diet of the black sea bream using the phosphorus gradient method (with casein as basic diet, sodium dihydrogen phosphate as source of phosphorus, and calcium lactate as source of calcium) showed that growth was greatly affected by the diet's phosphorus content and Ca/P ratio. Inadequate phosphorus in the diet resulted in slow growth and poor food conversion ratio (FCR). Analyses of the fish body showed it contained a high level of lipid but a low level of moisture, ash, calcium and phosphorus. The optimal values of phosphorus and Ca/P ratio in the black sea bream diet are 0.68% and 1:2 respectively. Phosphorus in excess of this optimum value resulted in slow growth or even death. The results of this experiment clearly indicated that phosphorus is the principal mineral additive affecting black sea bream growth. 相似文献
2.
An experiment on the optimum dietary protein content for the black sea bream using semi-purified diets showed the optimum
is 50.19% by weight as inferred from the growth rate and food consumption of the fish.
Another experiment on the proper ratio of animal to plant protein in its diet using sandeel as the animal protein source and
peanut cake meal as plant protein source showed that the ratio had great effect on its growth and should be 1.00: 0.89–0.96
as judged from the growth rate and food consumption of the fish.
Contribution No. 1973 from the Institute of Oceanology, Chinese Academy of Sciences. 相似文献
3.
An experiment on the optimum dietary protein content for the black sea bream using semi-purified dietsshowed the optimum content is 50.19% by weight as inferred from the growth rate and food consumptionof the fish. Another experiment on the proper ratio of animal to plant protein in its diet using sandeel as theanimal protein source and peanut cake meal as plant protein source showed that the ratio had great ef-fect on its growth and should be 1.00:0.89—0.96 as judged from the growth rate and food consumptionof the fish. 相似文献
4.
An experiment was performed to determine the dietary phosphorus requirement of the young abalone,Haliotis discus hannai. Five semi-purified diets were formulated to provide a series of graded levels of dietary total phosphorus (0.23%–1.98) from
monobasic potassium phosphate (KH2P04). The brown alga,Laminaria japonica, was used as a control diet. Similar size abalone were distributed in a single-pass, flow-through system using a completely
randomized design with six treatments and three replicates each treatment. The abalone were hand-fed to satiation with appropriate
diets in excess, once daily at 17:00. The feeding trial was run for 120-d. Survival rate and soft-body to shell ratio (SB/S)
were constantly maintained regardless of dietary treatment. However, the weight gain rate (WGR), daily increment in shell
length (DISL), muscle RNA to DNA ratio (RNA/DNA), carcass levels of lipid and protein, soft-body alkaline phosphatase (SBAKP),
and phosphorus concentrations of whole body (WB) and soft body (SB) were significantly (ANOVA, P<0.05) affected by the dietary
phosphorus level. The dietary phosphorus requirements of the abalone were evaluated from the WGR, DISL, and RNA/DNA ratio
respectively, by using second-order polynomial regression analysis. Based on these criteria, about 1.0%–1.2% total dietary
phosphorus, i.e. 0.9%–1.1% dietary available phosphorus is recommended for the maximum growth of the abalone.
Project 39670572 supported by the NSFC. 相似文献
5.
Requirement for dietary n-3 HUFA (n-3 highly unsaturated fatty acid) for growth and survival of black sea bream (Sparus macrocephalus) larvae was studied using rotifers and Artemia at various levels of n-3 HUFA. Five treatments with rotifers and Artemia differing in n-3 HUFA were prepared by enriching them with various oil emulsions. Results indicated that dietary n-3 HUFA significantly influence fish n-3 HUFA levels and are essential for growth and survival of black sea bream larvae. The results also indicated that the incorporation of n-3HUFA TG (triacylglycerols) into tissues of larval black sea bream was more effective from natural fish oil in comparison with n-3 HUFA fatty acid ethyl esters from ethyl-esterified oil. 相似文献
6.
Requirement for dietary n−3 HUFA (n−3 highly unsaturated fatty acid) for growth and survival of black sea bream (Sparus macrocephalus) larvae was studied using rotifers andArtemia at various levels of n−3 HUFA. Five treatments with rotifers andArtemia differing in n−3 HUFA were prepared by enriching them with various oil emulsions. Results indicated that dietary n−3 HUFA
significantly influence fish n−3 HUFA levels and are essential for growth and survival of black sea bream larvae. The results
also indicated that the incorporation of n−3HUFA TG (triacylglycerols) into tissues of larval black sea bream was more effective
from natural fish oil in comparison with n−3 HUFA fatty acid ethyl esters from ethyl-esterified oil.
Project No. 39770588 supported by the NSFC. 相似文献
7.
The effect of dietary phosphorus on the growth and body components of juvenile Synechogobius hasta was determined. Different percentages of dietary phosphorus (0.63, 0.77, 0.93, 1.06, 1.22 and 1.36) were tested by feeding
the fish (body weight, 15.81 g ± 0.32 g; 20 individuals each group; 3 groups each percentage) at a surplus of 5%–10% above
satiation for 35 d. Dietary phosphorus did not significantly affect the specific growth rate, feed intake, feed conversion
ratio and protein efficiency rate. Nitrogen retention was found to be the highest in fish fed the diet containing 1.06% of
phosphorus; however, this was not significantly different from other diets. Fish fed the diet containing 0.93% of phosphorus
showed the highest phosphorus retention; similar phosphorus retention rates were found in fish fed the diets containing 0.77%
and 1.06% of phosphorus. Fish fed the diet containing the highest percentage of dietary phosphorus were found to contain the
least whole body lipid, lower than fish fed other diets (P<0.05). The protein content increased from 18.59% to 19.55% (although not significant) with the decrease of body lipid content
(P>0.05). The contents of the whole body ash, whole body phosphorus and vertebrae phosphorus increased with dietary phosphorus
percentage up to 1.06 (P<0.05), reaching a plateau after that. Dietary phosphorus did not significantly influence the muscle components (protein,
lipid and moisture). Condition factor and hepatosomatic index were the highest in fish fed the diet containing 0.63% of dietary
phosphorus; however, this was not significantly different from those of other diets. The second-order polynomial regression
of phosphorus retention against dietary phosphorus identified a breakpoint at 0.88% of dietary phosphorus. However, the dietary
requirement of phosphorus for maintaining maximum phosphorus storage determined by broken-line analysis of the contents of
whole body phosphorus, and ash and vertebrae phosphorus was 1.06% of the diet. 相似文献
8.
A 54-day feeding experiment was conducted on juvenile Nile tilapia using isonitrogenous, isocaloric semipurified diets. The
carbohydrate content in the diet was 9%, 32% and 50%; the corresponding lipid content was 22.2%, 12%, and 4%. The diets were
supplemented with 0.85% or 1.5% phosphorus and 40 mg/kg or 100 mg/kg zinc. The experiment was carried out in flow-through
aquaria using dechlorinated tap water at 23–26°C.
The experiment showed that the increase of the carbohydrate content in the diets resulted in a 43–249% increase in weight
gain, a 27–59% decrease in feed conversion ratio, and a 65–121% increase in protein efficiency ratio. In fish fed diets containing
36–50% carbohydrate, an increase in supplemented phosphorus to 1.5% greatly increased the weight gain. On the contrary, a
high content of supplemented zinc (100 mg/kg) inhibited growth and increased feed conversion ratio. 相似文献
9.
the effects of recombinant eel growth hormone (reGH), methyltestosterone (MT) and L-thyroxine (T4) on the growth of red sea
bream,Pagrosomus major, were investigated. Administration of reGH to fry by immersion at 2 mg/1 for 2 h every 5 days resulted in significant increase
in both weight and length, but the condition factor (CF) diminished relative to that of similarly treated controls over the
37 day treatment period. Immersion in 0.1 mg/l T4 also resulted in significant increase in both weight and length and higher
survival rate of test fry compared to the controls. Immersion in MT had less effect on growth and high-dose resulted in high
mortality. In the second study, injection of 2 μg reGH/(g·wk) caused a significant increase in the specific growth rate (SGR)
of test red sea bream fingerlings relative to that of the controls during the 4—week treatment period and maintained the increasing
trend over the post-treatment period (weeks 4–6). Injection of MT at a dosage of 1μg/(g·wk) resulted in a significant increase
in SGR during the 4—week treatment period. Intramuscular injection of 0.1 μg T4/(g·wk) also resulted in a significant increase
in weight. Injection of high-doses of MT and T4 inhibited growth and resulted in darkening skin, bulging eyes and thinning
body. In muscle chemical composition, the treated groups had no significant differences compared to the controls, but the
high-dose MT and T4 groups showed significant increases in lipid content. Potential practical methods for hormone application
in fish culture are discussed. 相似文献
10.
The ratio of nitrogen/phosphorus (N/P) is known to affect cell proliferation of some marine micro algae. We evaluated the effect of N/P ratios on the proliferation and succession of phytoplankton using five marine micro algae species. We used two sources of nitrogen, NH4Cl (N1) and urea (N2), and a single source of phosphorous, NaH2PO4(P). The optimal N/P ratio that differed among the five species was affected by the source of nitrogen, being as follows (N1/P, N2/P in order): Thalassiosira sp. (30/1, 20/1),... 相似文献
11.
Laboratory culture experiments showed that <100μ mol/L nitrate, amonium or mixture of amino acids promote the growth of the
red tide organismProrocentrum micans Ehrenb, but that >100μmol/L of ammonium, or mixture of glycine and glutamate was harmful to growth, and that orthophosphate
wasP. micans’ main phosphorous source in the ocean. Presence of 80μ mol/L EDTA, 0.5 to 1 μmol/L Fe3+, 1.0 to 20.0 μ mol/L Mn2+ 0.1 to 0.4 μmol/L Co2+ in the culture medium could improve the growth ofP. micans. Vitamin B1 promoted growth, but vitamin B12 and biotin did not. The estimated minimum cell quotas (q
o) for nitrogen and phosphorus being 0.74 pmole/cell and 0.045 pmole/cell show that phosphorus (more than nitrogen) limits
the growth ofP. micans in the study area.
This project was supported by the Natural Science Foundation of Zhejiang Province. 相似文献
12.
Microalgal lipids are regarded as main future feedstock of biofuels for its higher efficiency of accumulation and sustainable production.In order to investigate the effect of various nitrogen to phosphorus ratios on cells growth,chlorophyll content and accumulation of lipids in Dunaliella tertiolecta,experiments were carried out in modified microalgal medium with inorganic nitrogen(nitrate-nitrogen) or organic nitrogen(urea-nitrogen) as the sole nitrogen source at initial N:P ratios ranging from 1:1 to 32:1.The favorable N:P of 16:1 in the nitrate-N or urea-N medium yielded the maximum cell density and specific growth rate.Decrease in chlorophyll content were observed at the N:P of 4:1 in both nitrate-N and urea-N cultures.It was also observed that the maximum lipids concentration was obtained at the N:P of 4:1 in both nitrate and urea nutrient medium.The lipid productivity and lipid content of cultures in the urea-N medium at the N:P of 4:1were markedly higher than those from cultures with other N:P ratios(p 0.05).The results of this work illustrate the possibility that higher ratios of nitrogen to phosphorus have enhancing effect on cells growth of D.tertiolecta.Conversely,higher lipid accumulation is associated with a decrease in chlorophyll content under lower ratios of nitrogen to phosphorus.The results confirm the hypothesis of this study that a larger metabolic flux has been channeled to lipid accumulation in D.tertiolecta cells when the ratios of nitrogen to phosphorus drop below a critical level. 相似文献
13.
植酸酶对凡纳滨对虾生长性能和体成分的影响 总被引：1，自引：0，他引：1
在凡纳滨对虾饲料中添加不同量微生物植酸酶(0,500,1000,2000 U/kg),观察虾的存活率、增重率、饲料系数及虾体和虾壳成分,研究饲料中添加植酸酶对凡纳滨对虾生长性能的影响。结果表明:饲养8周后,饲料中添加500~2000 U/kg植酸酶对凡纳滨对虾增重率、存活率和饲料利用无显著影响(P>0.05);植酸酶添加各组与对照组虾体的水分、粗蛋白、脂肪、灰分、总钙和总磷含量差异不显著;虾壳中粗灰分和钙含量在500和1000 U/kg组显著高于对照组和2000 U/kg组(P<0.05),虾壳磷含量在各饲料组间差异不显著。血清磷浓度在对照组和2000 U/kg组显著高于500 U/kg组(P<0.05),血清钙浓度在组间差异不显著,血清碱性磷酸酶活性以对照组最高,显著高于500和1000 U/kg组。结果说明,在特定试验条件下,饲料中添加植酸酶对凡纳滨对虾幼虾的虾壳和血清成分有显著影响(P<0.05),对幼虾生长性能影响不显著(P>0.05)。 相似文献
14.
The ratio of nitrogen/phosphorus (N/P) is known to affect cell proliferation of some marine micro algae. We evaluated the
effect of N/P ratios on the proliferation and succession of phytoplankton using five marine micro algae species. We used two
sources of nitrogen, NH4Cl (N1) and urea (N2), and a single source of phosphorous, NaH2PO4(P). The optimal N/P ratio that differed among the five species was affected by the source of nitrogen, being as follows (N1/P, N2/P in order): Thalassiosira sp. (30/1, 20/1), Heterosigma akashiwo (30/1, 30/1), Chroomonas salina (20/1, 30/1), Chaetoceros gracilis (40/1, 60/1), and Alexandrium sp. (10/1, 30/1). Thus, the source of nitrogen must be considered when analyzing the N/P ratio. Our results provide insight
for predicting phytoplankton succession in coastal waters and may be used to forecast the potential risk of harmful algal
blooms. 相似文献
15.
The gonads of sea urchins(Strongylocentrotus intermedius) are characterized by high levels of arachidonic acid(ARA, 20:4 n-6) and eicosapentaenoic acid(EPA, 20:5 n-3). However, to our knowledge, little information is available regarding the physiological response of adult sea urchins to dietary ARA. In the present study, four dietary feeds were formulated with graded ARA(0, 0.5%, 1%, and 2% dry diet). Each diet was randomly allocated to three cages during a 56-day feeding experiment. The results showed that the sea urchin weight gain rate(WGR) and the gonadosomatic index(GI) significantly increased as ARA was equal to or above 1.0% of dry diet(P 0.05). The activities of superoxide dismutase(SOD), catalase(CAT), and total anti-oxidative capacity(T-AOC) were the highest in the coelomic fluid of sea urchins that were fed diets with 1% ARA. The total essential amino acid(TEAA) and its ratio to total non-essential amino acid(TNEAA) showed a similar tendency to WGR and GI as dietary ARA increased, and the highest TEAA and TEAA/TNEAA were observed in the gonads of sea urchins that were fed diets with 1% ARA. Levels of ARA and ARA/EPA of the gonads increased while n-3/n-6 polyunsaturated fatty acid(PUFA) decreased with the increase of dietary ARA(P 0.05). EPA in the gonads of experimental animals fed with formulated feeds showed no significant differences(P 0.05), but was significantly lower than those fed with kelp(P 0.05). These results suggested that relatively higher levels of ARA(1% dry diet) significantly promoted growth, gonad development, activities of antioxidant enzymes, as well as nutritional values(TEAA, TEAA/TNEAA, and PUFA) of adult S. intermedius. 相似文献
16.
Synchrotron radiation X-ray fluorescence （SR-XRF） approach was applied to analyzing of Chinstrap penguin （ Pygoscelis Antarctica） cortical bone. The method enabled the in situ determination of Ca and P concentrations and the Ca/P ratio in cortical bone. The preliminary results show that ： （ 1 ） there is the bone site-related difference for Ca and P concentrations. The mean values for the investigated parameters （ on a dry-weight basis） are： 30.7% （Ca） and 14.9% （P） for the femoral cortical bone, 21.4% （Ca） and 11.5% （P） for wing cortical bone. （2） The variation for the Ca/P ratio in cortical bone is lower than those for Ca and P separately. This is in agreement with the previous report that the specificity of the Ca/P ratio is better than that of Ca and P concentrations and is more reliable for the diagnosis of bone disorders. The authors suggest that further studies be conducted to establish norreal values of Ca, P and Ca/P ratio for polar animals and provide a basis for the diagnosis of bone disorders. 相似文献
17.
AVAILABILITY OF PHOSPHORUS FROM SELECTED INORGANIC PHOSPHATES TO JUVENILE ABALONE, HALIOTIS DISCUS HANNAI INO 总被引：2，自引：0，他引：2
The availability of phosphorus to juvenile abalone,Haliotis discus hannai from primary, secondary and tertiary calcium phosphate, primary sodium or potassium phosphate separately or in combination
was determined in a 120-day feeding trial. Seven semi-purified diets were formulated to contain relatively constant dietary
phosphorus, ranging from 0.78%–0.82%. The concentrations of dietary phosphorus were presumed to be slightly below the requirement
for abalone. A brown alga,Laminaria japonica, was used as a control diet. Abalone juveniles of silimar size (average weight 1.18 g; average shell length 18.74 mm) were
distributed in a single-pass, flow-through system using a completely randomized design with eight treatments and three replicates
each treatment. The survival ranged from 71.1% to 81.1%, and was not significantly (P>0.05) related to dietary treatment.
However, the weight gain rate (41.72%–65.02%), daily increment in shell length (36.87–55.07 μm) and muscle RNA-DNA ratio (3.44–4.69)
were significantly (P<0.05) affected by dietary treatment. Soft body alkaline phosphatase activity (10.9–19.8 U/g wet tissue)
and carcass levels of lipid (7.71%–9.33%) and protein (46.68%–49.35%) were significantly (P<0.05) responsive to available
phosphorus of the diets. Dietary treatment had significant effect (P<0.05) on concentrations of phosphorus in the whole body
(WB) and soft body (SB). Apparent digestibility coefficients (45%–97%) of phosphorus were also significantly (P<0.05) different
between dietary treatments. The results indicated that among these inorganic compounds, primary calcium, potassium and sodium
phosphates separately or in combination could be utilized effectively by juvenile abalone as dietary phosphorus sources (availability
ranging from 87%–97%). However, secondary and tertiary calcium phosphates were found to be low in availability, the values
being 45% and 77%, repectively.
Project 39670572 supported by the Natural Science Foundation of China (NSFC). 相似文献
18.
In Antarctica, the marine ecosystem is dynamically interrelated with the terrestrial ecosystem. An example of the link between these two ecosystems is the biogeochemical cycle of phosphorus. Biovectors, such as penguins, transport phosphorus from sea to land, play a key role in this cycle. In this paper, we selected three colonies of penguins, the most important seabirds in Antarctica, and computed the annual quantity of phosphorus transferred from sea to land by these birds. Our results show that adult penguins from colonies at Ardley Island, the Vestfold Hills, and Ross Island could transfer phosphorus in the form of guano at up to 12 349, 167 036, and 97 841 kg/a, respectively, over their breeding period. These quantities are equivalent to an annual input of 3.96×109–1.63×1010 kg of seawater to the land of Antarctica. Finally, we discuss the impact of phosphorus on the ice-free areas of the Antarctica. 相似文献
19.
Jiahuan Rong Yu Han Shanjie Zha Yu Tang Wei Shi Xiaofan Guan Xueying Du Maolong He Guangxu Liu 《中国海洋大学学报(英文版)》2020,19(2):428-438
Recently,the use of natural immunopotentiators,such as plant extracts,is predicted to offer high disease-prevention potential in aquaculture.However,few reports on the immunomodulatory impacts of olive extract(OE)on teleost are available.Therefore,the effects of dietary intake of triterpene-enriched OE on black sea bream(Acanthopagrus schlegelii)were investigated in this study.Our data showed that total blood cell counts,in vivo content of lysozyme,activities of antiprotease and myeloperoxidase,and contents of IL-4 and IL-6 were significantly up-regulated by dietary intake of triterpene-enriched OE.Additionally,the hampered immune response induced by cadmium exposure was significantly mitigated by the administration of OE as were indicated by partially or completely rescued immune-relating parameters.Furthermore,the expressions of immune-related genes encoding NF-κB inhibitor alpha(IkBα),tumor necrosis factorα(TNFα),cyclooxygenase-2(COX-2),and proto-oncogene protein c-fos(FOS)were found to be significantly up-regulated by the dietary intake of OE.In general,the results suggested that the dietary intake of triterpene-enriched OE has immune enhancing effect in black sea bream.Such effect may be realized by 1)increasing the total counts of diverse blood cells;2)activating nonspecific immune biomolecules;and 3)affecting signaling pathways such as IKK and ERK and subsequently inducing IL-4 and IL-6 biosyntheses. 相似文献
20.
《中国海洋大学学报(英文版)》2018,(6)
This study was conducted to evaluate the effects of plant/animal(P/A) protein ratios(viz.1:4, 1:3, 1:2, 1:1,2:1, 3:1, 4:1) on growth performance, body composition, apparent digestibility of diets, and nonspecific immunity of juvenile sea cucumber(Apostichopus japonicus). Sea cucumbers were divided into 21 plastic tanks, and each tank was stocked with 15 individuals(initial weight: about 23.73 g). Each feed was allocated to three replicates of sea cucumbers. The feeding experiment lasted for 50 days. Results indicated that weight gain rate(WGR) and body wall weight(BWW) significantly increased as dietary ratio of P/A increased from 1:4 to 3:1, and then decreased significantly with further increase of this ratio(P 0.05). The body wall coefficient(BWC) showed a similar tendency to WGR and BWW, but no significance was detected among dietary treatments(P 0.05). The apparent digestibility of dry matter, protein and lipid increased with ratio of P/A increasing from 1:4 to 2:1(P 0.05), and then decreased with further increase of this ratio. Correspondingly, activities of trypsin and amylase were significantly increased as P/A increased from 1:4 to 2:1(P 0.05). The activities of SOD and CAT showed a similar trend with WGR, with the highest value observed in the ratio of 1:2 and 1:1, respectively. Results above showed that moderate or relatively higher ratio of P/A protein(1:1-3:1) significantly increased the growth performance, apparent digestibility, and nonspecific immunity of sea cucumber. This will contribute to improving the feed formulation for juvenile cucumbers. 相似文献