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1.
眼柄粗提物对凡纳滨对虾性分化的影响 总被引：1，自引：0，他引：1
采用生化方法制备凡纳滨对虾[Litopenaeus(Penaeus)vannamei)]眼柄粗提物,用活体注射法研究不同粗提物剂量对去眼柄凡纳滨对虾卵母细胞直径大小的影响,以检验其性腺抑制素(Gonadinhibiting hormone,GIH)的生物活性;在凡纳滨对虾仔虾后17、22和27 d,分别以不同浓度眼柄粗提物浸泡24 h,结果显示:除0.001个眼柄/mL的GIH浓度外,0.01、0.1和1个眼柄/mL组均显著降低凡纳滨对虾卵母细胞直径(P﹤0.05);浸泡处理后,仔虾后17 d GIH处理组凡纳滨对虾雌性率达70.1%～80.1%;仔虾后22 d GIH处理组雌性率达65.8%～71.7%,各浓度GIH处理组雌性率比对照组(雌雄比例约为1:1)显著提高(P﹤0.05);仔虾后27 d,0.01和1个眼柄/mL处理组雌性率(约58%)略高于对照组,但0.1个眼柄/mL处理组雌性率(73.1%)与对照组相比显著提高(P﹤0.05)。结果表明较低浓度的眼柄粗提物可明显诱导凡纳滨对虾雌性化,在仔虾后22 d前处理效果最佳。 相似文献
2.
《广东海洋大学学报》2016,(3)
为探讨眼柄切除对凡纳滨对虾性成熟的促进机理,采用镊烫法切除雌性凡纳滨对虾单侧眼柄,并测量肝胰腺指数和性腺指数,测定卵巢、肝胰腺和血清中生化组分含量的变化。结果表明,切除眼柄7 d后,1)肝胰腺指数降低,性腺指数上升,差异均有统计学意义(P0.05);2)卵巢、肝胰腺中总蛋白含量增加,差异有统计学意义(P0.05),血清中总蛋白含量无明显改变(P0.05);3)卵巢和血清中胆固醇含量增加,肝胰腺中胆固醇含量降低,差异均有统计学意义(P0.05);4)血清中钙离子和无机磷含量增加,差异有统计学意义(P0.05)。切除眼柄后,卵巢和肝胰腺中卵黄蛋白原等营养物质大量合成,肝胰腺中的总蛋白、胆固醇等营养物质先转移至血淋巴,再转移至卵巢,从而促进性腺的成熟。 相似文献
3.
本文简要地介绍了建立沿海渔业天气警报服务系统的必要性,系统的设计特点,现场试验情况以及今后建台布网的设想。特别是把短波单边带通信,地面波传播方式和双音报警技术引入天气警报服务系统,开辟了短波单边带气象通应用的新领域。 相似文献
4.
刘永 《广东海洋大学学报》2002,22(4):19-23
研究了海水比重的变化对凡纳对虾亲虾人工繁殖活动的影响及换水对亲虾交配率的影响。试验采用剪眼柄、强化培育、控光、换水等技术措施 ,将凡纳对虾培育成熟并使之自然交配产卵 ,孵化出无节幼体 ;亲虾的平均产卵量可达 1 6万粒左右 ,卵的受精孵化率可达 80 %以上。结果表明 :适时换水可将亲虾的交配率从 4 6 %提高到 79% ;凡纳对虾人工繁殖的适宜海水相对密度为 1 0 1 6以上。 相似文献
5.
中国社科院蓝皮书:地方政府土地财政推高房价 总被引：2，自引：0，他引：2
<正>在经营城市的诱惑下,地方政府通过各种手段推高地价和房价,以实现土地收益最大化,城市房价在地方政府的推动下单边上涨,居高不下,使多数城市居民难以承受。这种状 相似文献
6.
凡纳对虾人工繁殖的初步研究 总被引：2，自引：0，他引：2
刘永 《广东海洋大学学报》2002,22(4):19-23
研究了海水比重的变化对凡纳对虾亲虾人工殖活动的影响及换水对亲虾交配率的影响，试验采用剪眼柄，强化培育，控光，换水等技术措施，将凡纳对虾培育成熟并使之自然交配产卵，孵化出无节幼体；亲虾的平均产卵量可达16万粒左右，卵的受精卵化率可达80％以上，结果表明，适时换水可将亲虾的交配率从46％提高到79％，凡纳对虾人工繁殖的适宜海水相对密度为1．016以上。 相似文献
7.
基于不确定度理论,建立参数有界约束下的平差模型。为克服不等式约束模型单边约束的缺陷,从最小二乘出发,将问题转化为附有箱型约束的二次规划问题,提出一种求解参数最优估值的新算法,并给出参数估计的统计性质。数值实验表明,新算法简单、可行,具有较快的收敛速度,并能够在一定程度上减少部分数值的部分不确定性。 相似文献
8.
李良才 《广东海洋大学学报》2009,29(5):34-38
WTO多边贸易体制倡导自由贸易。WTO成员之间应当削减或取消一切贸易壁垒,共同承担多边自由贸易的义务。美国根据国内贸易法采取的人权措施具有单边性、任意性并且暴露了此类贸易限制或禁止措施的霸权主义本质。美国人权措施引发了诸多贸易争端,严重挑战自由贸易精神。美国对华实施人权措施造成我国对美出口遭遇不利影响。我国应从革新外贸立法理念、增设人权措施条款并适度运用人权措施等方面积极应对此类新型贸易壁垒。 相似文献
9.
《广东海洋大学学报》2020,(4)
【目的】研究凡纳滨对虾(Litopenaeus vannamei)miRNA-184 [microRNA-184(miR-184),记作LvmiR-184]在健康虾及染病虾中的表达,探讨其免疫功能。【方法】用茎环法克隆鉴定凡纳滨对虾LvmiR-184,通过荧光定量PCR检测LvmiR-184在对虾血淋巴、肝胰腺、鳃、肌肉、心脏、胃、肠和眼柄等8个组织中的分布。对凡纳滨对虾注射副溶血弧菌(Vibrio parahaemolyticus),检测LvmiR-184不同时间点的响应变化。对感染副溶血弧菌的凡纳滨对虾分别注射0.3、3.0和30.0 ng的mi R-184模拟物和抑制物,检测不同时间点对虾血细胞凋亡率。【结果与结论】LvmiR-184在所有组织中均有表达,在胃中表达量最高,其次是肌肉,鳃表达量最低。注射副溶血弧菌后1.5 h,LvmiR-184表达量显著上调;在24 h达到峰值,较对照组高8.25倍;对照组则维持在较低的水平。模拟物、抑制物注射量为30 ng时对虾血细胞凋亡率差异有统计学意义(P 0.05);与对照组凋亡率相比,注射模拟物6 h后提高62%。LvmiR-184可调控凡纳滨对虾的血细胞凋亡。 相似文献
10.
《国土资源》2013,(1):26-30
2007年4月履新国土资源部部长、党组书记、国家土地总督察的徐绍史,执政轨迹迄今已跨越十六大后期、十七大全程和十八大序期.
前后近6年间,恰值中国工业化、城镇化加速发展,又逢房地产市场持续单边上扬、及至调控震荡.在科学发展观的统领下,国土资源部门被赋予的新历史使命,是节约集约利用土地,深化土地制度改革、增强制度创新与供给.
徐绍史对历史赋予其自身的角色和使命有清醒认知.在《关于2012年深化国土资源管理改革创新重点工作的意见》中,徐绍史曾对他任期内的任务总结道:"深化改革创新,加快新的制度供给,逐步健全有利于保障和促进科学发展的新型的国土资源管理制度框架体系,是全系统的一项重大任务". 相似文献
11.
应用因子分析法研究密鲴繁殖力与诸因素的关系。六个变量是体长、体重、成熟系数、绝对怀卵量和相对怀卵量。29个样本、经正交变换得到3个因子:绝对繁殖因子、相对繁殖因子和成熟因子。 相似文献
12.
LOCALIZATION OF A PUTATIVE EPIBOLY-DETERMINING FACTOR IN OOCYTES OF THE GOLDFISH (CARASSIUS AURATUS)
It is unknown whether cytoplasmic determinants in goldfish eggs are asymmetrically localized beforematuration. In this study fully grown goldfish oocytes with infact germinal vesicles (GVs) were ligated withbaby hair loops along desired planes into two parts, mature in vitro, and inseminated. Embryos developedfrom the animal halves with GV of oocytes ligated along the equator un unable to undergoepiboly, while those developed from the GV-containing parts of oocytes ligated along the animal-vegetal axiswere able to. Also, embryos developed from the animal halves obtained from fertilized eggs could undergo epiboly. From there results, it was postulated that the goldfish's premature oocyte possessed a putativefactor in the vegetal hemisphere which was responsible for epiboly of the embryonic cells. 相似文献
13.
Serotonin (5-HT) has been found to stimulate meiotic maturation of oocytes in many molluscs. During maturation, several signaling pathways are involved, especially the phosphatidylinositol and cAMP pathways. In order to examine the possible role of the phosphatidylinositol signaling pathway in regulating oocyte maturation in Mercenaria mercenaria, the effects of the activator/inhibitor of phospholipase (PLC) and protein kinase C (PKC) on serotonin-induced maturation were studied. Results show that high-concentrations of neomycin (inhibitor of PLC) blocked oocyte maturation, while 9, 10-dimethyl-1, 2-benzanthracene (DMBA, activator of PLC) promoted oocyte maturation in the presence of serotonin. It was also found that in the presence of serotonin, phorbol 12-myristate 13-acetate (PMA, activator of PKC) inhibited oocyte maturation, while sphingosine (inhibitor of PKC) stimulated oocyte maturation. These results indicate that serotonin-induced oocyte maturation requires the activation of the phosphatidylinositol pathway. Decrease of PLC inhibited serotonin-induced oocyte maturation, whereas a decrease of PKC stimulated the maturation. Thus, our study indicates that serotonin promotes maturation of M. mercenaria oocytes through PLC stimulated increase in calcium ion concentration via inositol-1, 4, 5-trisphosphate (IP3) but not PKC. 相似文献
14.
1-methladenine (1-MA) induces starfish oocytes maturation via surface reaction followed by the appearance of a cytoplasmic
maturation factor which in turn induces germinal vesicle breakdown (GVBD) to resume meiosis. Cellular mechanisms involved
in GVBD were investigated by microinjection of metabolic inhibitors. Colchicine (Co) inhibited maturation, cytochalasin-B
(CB) delayed GVBD and actinomycin-D-(Act-D) and puromycin (Pu) had no effect. It appears that the microtubule and the microfilament
systems are associated with the nuclear membrane dissolution during the process of oocyte maturation of starfish. 相似文献
15.
Wang Renxue Wu Xianhan Zhou Jing Zhang Shicui Ma Yingjie Wu Shangqin Shi Yingxian 《中国海洋湖沼学报》1991,9(1):88-93
Full maturation of goldfish oocyte was inducedin vitro by 17 α-hydroxy-20β-dihydroprogesterone. The oocyte maturation involves GV migration to the periphery of the oocyte and germinal
vesicle breakdown (GVBD). In the experiment, incubation duration for GVBD varied in different broods of oocytes. Generally,
if the duration for GVBD was shorter than 6 h, oocytes would have a better chance to survive after maturation and insemination.
The maturation of nucleus (GV) and cytoplasm are not synchronous. Cytoplasm maturation occurs several hs after GVBD. Oocytes
inseminated 8–9 h after GVBD have the highest fertilizing and hatching rate. Fertilized ova maturedin vitro can develop to sexually mature adults capable of reproduction.
Contribution No 1731 from the Institute of Oceanology, Academia Sinica. 相似文献
16.
The limited knowledge about the relationships between environmental factors and the maturation and reproductive seasonality of nemerteans is mostly obtained from field studies, and in many cases is conjectural. Few species have been studied under well-controlled laboratory conditions. In this work, the effects of temperature and photoperiod on gonad development and spawning of Cephalothrix hongkongiensis were observed by rearing worms under different temperatures and photoperiods. Worms reared at 4℃ and 6℃ showed no growth or gonadal development, and all died within 109 days. At 8℃ and 12℃, gonads could be seen from 90 days to the termination of the 360-day experiment, but no spawning was observed. Worms maintained at 16, 24, and 28℃ showed both gonadal development and spawning, and matured faster at higher temperatures. Under a fixed temperature(16℃), photoperiod did not have any significant impact on maturation and spawning. It is likely that temperature plays a major role in regulating the maturation and reproductive cycle of C. hongkongiensis. 相似文献
17.
引进印度尼西亚大珠母贝人工育苗试验 总被引：2，自引：0，他引：2
在雷州半岛海域水温24.0～30.0℃、盐度30.8～32.5、pH8.0～8.3条件下,以牟氏角毛藻,亚心形扁藻,牟氏角毛藻、亚心形扁藻和小球藻,天然混合藻等4种饵料,进行印度尼西亚产大珠母贝亲贝促熟培育试验。结果表明:不同饵料组合在亲贝促熟百分率、排精产卵率和受精率之间差异不显著(P>0.05);阴干温差流水刺激可以有效诱导亲贝排精产卵,优选幼虫和投放合适的采苗器可以提高幼虫的变态率。 相似文献
18.
We conducted a phenological evaluation of Sargassum thunbergii, a common algal species, along the coast of Lidao Bay, Rongcheng, China. The local S. thunbergii population entered a maturation period from early June to mid-August, when seawater temperature was 14.4 to 25.1°C. Our results suggest an increase in temperature and day length during this period initiated thalli maturation. Inhabitants of the low tidal zone had higher thalli length and biomass compared with those from the mid-and high tidal zones. We observed a switch in the length frequency distribution and contribution of length classes to biomass between the small (<20 cm) and long (20–79.9 cm) length classes at the time of thallus maturity. This suggests there is a trade-offbetween sexual reproduction and vegetative regeneration. 相似文献
19.
The in vitro effects of steroids, human chorionic gonadotropin (HCG) and cyanoketone on germinal vesicle breakdown (GVBD)
of striped mullet (Mugil cephalus L.) oocytes were investigated. All concentrations of HCG (5,10,50 I.U./ml), progesterone and pregnenolone at the highest
concentrations(lug/ml) were moderately effective in inducing GVBD, whereas 17β-estrodiol, cortisol, testosterone, 11β-hydroxyandrostenedione
and 11-ketotestosterone did not stimulate GVBD. 17α, 20βdihydroxy-4-pregnen-3-one (17α, 20βdiOHprog) and deoxycorticosterone
(DOC) were the most potent steroids in stimulating final oocyte maturation. The results indicate that C21 hydroxylated steroids
are potent inducers of final maturation in mullet. Further, co-incubations with 17β-estradiol, cortisol and testosterone did
not alter the maturation-inducing effects of HCG or 17α,20βdiOHprog. Cyanoketone, a blocker of 3βHSD activity, was only partially
effective in blocking GVBD induced by HCG. This suggests that Δ5 (pregnenolone derived) and Δ4 steroids may be involved in
final oocyte maturation in this species. 相似文献
20.
KONG Lingfeng WANG Zhaoping YU Ruihai LI Qi WANG Rucai 《中国海洋大学学报(英文版)》2007,6(4):383-386
The glycogen content and the activities of two key enzymes in glycogen metabolism, glycogen phosphorylase and gly- cogen synthetase, in the gonad of diploid and triploid Pacific oysters (Crassostrea gigas) were compared during maturation. The glycogen content in the gonad of diploids decreased with gametogenesis (by 85.7%), but the glycogen content in the gonad of trip- loids did not vary significantly. Activity of glycogen phosphorylase (GP) in the gonad of diploids decreased with gametogenesis (by 55.5%), while GP activity of triploids did not vary significantly during maturation. Activity of glycogen synthetase (GS) in the gonad of diploids increased slightly with gametogenesis, reaching a peak in June. Activity of GS declined sharply from June to July, which might be due to gonad spawning. GS activity of triploid oysters in spawning time (July and August) was significantly higher than that in other months, which might be explained with a ‘compensating’ mechanism for the higher glycogen content in triploids. 相似文献