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We constructed and characterized a normalized cDNA library of Nannochloropsis oculata CS-179, and obtained 905 nonredundant sequences (NRSs) ranging from 431–1 756 bp in length. Among them, 496 were very similar
to nonredundant ones in the GenBank (E ≤1.0e-05), and 349 ESTs had significant hits with the clusters of eukaryotic orthologous groups (KOG). Bases G and/or C at
the third position of codons of 14 amino acid residues suggested a strong bias in the conserved domain of 362 NRSs (>60%).
We also identified the unigenes encoding phosphorus and nitrogen transporters, suggesting that N. oculata could efficiently transport and metabolize phosphorus and nitrogen, and recognized the unigenes that involved in biosynthesis
and storage of both fatty acids and polyunsaturated fatty acids (PUFAs), which will facilitate the demonstration of eicosapentaenoic
acid (EPA) biosynthesis pathway of N. oculata. In comparison with the original cDNA library, the normalized library significantly increased the efficiencies of random
sequencing and rarely expressed genes discovering, and decreased the frequency of abundant gene sequences. 相似文献
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Ubiquitin, a highly conserved stress-related protein, is assigned multiple functions, such as DNA processing, protein degradation, and ribosome synthesis. The Crassostrea hongkongensis ubiquitin gene (designated ChUbL40) was cloned by a combination of suppressive subtractive hybridization (SSH) and rapid amplification of cDNA ends (RACE). The full-length cDNA of ChUbL40 is 496 bp in length, consisting of a 5’ untranslated region (UTR) of 34 bp, a 3’-UTR of 75 bp and an open reading frame of 387 bp encoding a ubiquitin fusion protein of 128 amino acids. Analysis of the amino acid sequence of ChUbL40 reveals that UbL40 is highly conservative during evolution. The expression patterns of ChUbL40 gene in various tissues were examined by real-time PCR. The expression level of ChUbL40 in haemocytes is down-regulated at 4 h and gradually returned to its original level from 6 h to 24 h after Vibrio alginolyticus challenge. Our results suggest that ChUbL40 is ubiquitously expressed and plays an important role in immune defense against bacterial challenge. 相似文献
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MAHongming XUWei MAIKangsen LIUFUZhiguo CHENHong 《中国海洋大学学报(英文版)》2004,3(2):145-149
1 Introduction Actinisanimportantcontractileproteinineukary oticcells .Itisoneofthetwomajorcomponentsin volvedinthecontractionofmusclecells .Innon mus clecells ,itisthemajorpartofcytoskeletoninvolvedinmanyprocessessuchascellmotility ,endocytosis ,exocyt… 相似文献
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Ultraviolet mutagenesis was applied to Nannochloropsis oculata and three mutants resistant to 3-(3, 4-dichlorophenyl)-1,1-dimethylurea (DCMU) were isolated. The cellular chlorophyll a and total lipid content of the wild are higher in the medium supplemented with DCMU than in the control without DCMU. Without
DCMU, the growth rates and chlorophyll a contents of the mutants are similar to those of the wild. Significant changes of fatty acid content and composition have
occurred in DCMU-resistant mutants growing in the medium supplemented with DCMU. The total lipid, palmitic acid (16:0), palmitoleic
acid (16:1ω9) and oleic (18:1ω9) contents decrease significantly, while the vaccenic acid (18:1ω11) increases significantly
and the EPA content of dried powder increases slightly in the mutants. The study may provide a basis to improve EPA content
in Nannochloropsis oculata in the future. 相似文献
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Xiaolei Ma Kehou Pan Lin Zhang Baohua Zhu Guanpin Yang Xiangyang Zhang 《中国海洋大学学报(英文版)》2016,15(2):351-356
The gene ble from Streptoalloteichus hindustanus is widely used as a selective antibiotic marker. It can control the phleomycin resistance, and significantly increase the tolerance of hosts to zeocin. The unicellular marine microalga Nannochloropsis oculata is extremely sensitive to zeocin. We selected ble as the selective marker for the genetic transformation of N. oculata. After the algal cells at a density of 2×107 cells mL?1 was digested with 4% hemicellulase and 2% driselase for 1 h, the protoplasts accounted for 90% of the total. The ble was placed at the downstream of promoter HSP70A-RUBS2 isolated from Chlamydomonas reinhardtii, yielding a recombinant expression construct pMS188. The construct was transferred into the protoplasts through electroporation (1 kV, 15 μS). The transformed protoplasts were cultured in fresh f/2 liquid medium, and selected on solid f/2 medium supplemented with 500 ng mL?1 zeocin. The PCR result proved that ble existed in the transformants. Three transformants had been cultured for at least 5 generations without losing ble. Southern blotting analysis showed that the ble has been integrated into the genome of N. oculata. The ble will serve as a new dominant selective marker in genetic engineering N. oculata. 相似文献
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Optimal salinity for dominant copepods in the East China Sea, determined using a yield density model
From 1997 to 2000, four field surveys were conducted in the East China Sea (ECS) (23°30′–33°00′N, 118°30′–128°00′E). A field
data yield density model was used to determine the optimal salinities for 19 dominant copepod species to establish the relationship
between surface salinities and abundance of those species. In addition, ecological groups of the copepods were classified
based on optimal salinity and geographical distribution. The results indicate that the yield density model is suitable for
determining the relationship between salinity and abundance. Cosmocalanus darwini, Euchaeta rimana, Pleuromamma gracilis, Rhincalanus cornutus, Scolecithrix danae and Pareucalanus attenuatus were determined as oceanic species, with optimal salinities of >34.0. They were stenohaline and mainly distributed in waters
influenced by the Kuroshio or Taiwan warm current. Temora discaudata, T. stylifera and Canthocalanus pauper were nearshore species with optimal salinities of <33.0 and most abundant in coastal waters. The remaining 10 species, including
Undinula vulgaris and Subeucalanus subcrassus, were offshore species, with optimal salinity ranging from 33.0–34.0. They were widely distributed in nearshore, offshore
and oceanic waters but mainly in the mixed water of the ECS. 相似文献
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Based on a simple conceptual model of stratified ocean, the criterion of the geostrophic velocity inversion in and below the
thermocline was derived ash′·ν′<0 and {ie1-1}, meaning that the slopes of the thermocline (h′) and the sea {ie1-2} must be opposite to each other, and thath′ must be strong enough to satisfy the latter inequality. The criterion was applied to discuss the features of the western
boundary undercurrents, the counter undercurrents of the western boundary currents below the thermocline, and to discuss the
dynamics of their formation finally resulting from the combination of the basin-scale circulation and local geostrophic balance.
The formation mechanism, multicore structure, and transport variations of the Mindanao Undercurrent and those of other undercurrents,
such as the North Equatorial Undercurrent and the Kuroshio undercurrent, can be satisfactorily explained by the above results.
Research supported by the Postdoctoral Research Foundation and the NSFC (No. 49176255 and 49706066). Contribution No. 3405
from the Institute of Oceanology, Chinese Academy of Sciences. 相似文献
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Xinping Zhang 《中国地理科学(英文版)》1997,7(4):339-346
The relations between δ18O and temperature on the different time scales were analysed, according to the data from Tuotuohe (34°13′N, 96°25′E; 4533
m a. s. l.), Delingha (37°22′N, 97°22′E; 2981 m a. s. l.) and Xining (36°37′N, 101°46′E; 2261 m a. s. l.) in the Qinghai-Xizang
Plateau. The results show that the significance of d δ18O/dT on different time scales are different. The d δ18O/dT on the synoptic scale reflects the interdependent relation between δ18O and temperature in the short-term synoptic scale process; the d δ18O/d T on the seasonal scale reflects the relation between them whithin a year; and the d δ18O/d T on the climatic scale reflects the relation between them in the long-term climatic change. The calculated d δ18O/dT on climatic scale is very close to the theoretical values on the condition of advection transport for Tuotuohe Station. However,
there are great differences between the calculated and the theoretical values for Delingha and Xining stations. 相似文献
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Three new spades and a new variety of nannoplankton, Chrysochromulina papillata, Gaysochromulina chiton var. minuta, Paraphysomonas simplexocorbita and Paraphysomonas bisorbulina are reported in this paper. All were isolated from the preliminary culture samples of seawater collected from Jiaozhou Bay, Shandong, China. The three species occurred at Station 1(120° 14.56′ E, 36°4′N) in November 1984, the new variety at Station 2 (120° 16.35° E,36°4.5′N) in January, 1985. The morphological features, especially the structures of the scales of these new nannoplankton,. are described. The differences between the new species and the related ones are discussed; their movement and nutrition, and the temperature and salinity of their biotopes are also mentioned. 相似文献
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Shuo Li Chunmei Li Xubo Wang Yanan Wang Zhipeng Liu Teng Zhai Quanqi Zhang 《中国海洋大学学报(英文版)》2010,9(1):59-64
A novel immune-related gene was expressed in Japanese flounder (Paralichthys olivaceus) injected with Vibrio anguillarum. The complete cDNA contained a 169 bp 5’UTR, a 336 bp open reading frame (ORF) encoding 111 amino acids and a 556bp 3’UTR.
Six exons and five introns were identified in the PoIR2 gene. Blastp similarity comparison showed its encoding protein had 50% similarity to Danio rerio neuromedin S (NMS), but further alignment indicated they did not have NMS C-terminal conservational signature domain. So
it was not defined as an NMS homologue. Protein structure analysis indicated it had a 26aa signal peptide and was a secretory
pathway protein. RT-PCR demonstrated that the expression of PoIR2 was quickly induced and drastically increased in liver, kidney, spleen, gills, intestine, heart, and skeletal muscle after
infected with V. anguillarum. These results indicated that the PoIR2 might play some important role in Japanese flounder immune response system. This gene was named PoIR2 (P.olivaceus immune-related gene 2, GenBank accession number: EU224372). The mature PoIR2 peptide was expressed in BL21(DE3) pLysS using pET-32a(+) vector and a great part of the recombinant mature peptide existed
as soluble type. 相似文献
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The fatty acid compositions of seston and Calanus sinicus were investigated to study trophic relationships in Jiaozhou Bay. Principal component analysis was carried out to ordinate the fatty acid patterns of seston in stations and months. The results showed that diatoms were most abundant in the phytoplankton at station A5 (located in the northwest of the bay:36°9′N, 120°20′E) and least abundant at station D7 (located outside of the bay: 35°59′N, 120°26′E). By contrast, dinoflagellates were most abundant at station D7 and least abundant at station A5. According to the annual variations of 16:1ω7 and 18:4ω3/ 16:1ω7, diatoms flourished mainly in spring and summer, while dinoflagellates bloomed exclusively in summer. A distinctive feature of the fatty acid composition of C. sinicus was the prevalence of 20:5ω3 and 22:6ω3. The higher content of 16:1ω7 over 18:4ω3 in females indicated that diatoms contributed more than dinoflagellates to the diet of C. sinicus. The feeding intensity of C. sinicus on diatoms was higher in spring and autumn than in other seasons. The herbivorous indicators 20:1 and 22:1 were comparatively low, suggesting that besides phytoplankton, C. sinicus might feed on a wider range of particles including organic detritus, bacteria and small copepods. 相似文献
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During the 13th (1996–1997) and the 19th (2002–2003) Chinese National Antarctica Research Expeditions, we collected 60 discrete surface seawater samples along the
cruise from the Chanjiang River (Yangtze) estuary (30°59′S, 122°26′E) through Taiwan Strait, the South China Sea, and the
Eastern Indian Ocean to Prydz Bay, Antarctica (69°10′S, 74°30′E), and analyzed them for the 226Ra specific activity. The 226Ra specific activity of the Chanjiang River estuary surface water (3.15 Bq/m3) was found to be the highest among all the surface samples because of the desorption of 226Ra from riverine particles. Between Chanjiang River estuary and 40°S, 226Ra specific activity was found to be relatively uniform with a mean value of 1.07 Bq/m3 (n = 19, SD = 0.14), similar to that of the open ocean. From 40°S to 65°S, 226Ra specific activity increased intensively, then decreased moderately further southwards. Near the Antarctic shore, it increased
again, to 2.31 Bq/m3. This distribution was controlled by a combination of deep water upwelling, Southern Ocean fronts, water mixing and the continental
226Ra import. In Prydz Bay and the adjacent sea area, the mean 226Ra activity value was 2.26 Bq/m3 (n = 31, SD = 0.28), with a relatively higher value outside of the bay and low 226Ra activity value in the center of the bay. This was consistent with the topography and hydrological setting of the bay. In
addition, we extended the study area northward to the Arctic, by combining the published 226Ra dataset for surface water from the Bering Sea to the Japan Sea. We also discuss the 226Ra distribution of high latitude oceanic surface water and its mechanisms. 相似文献
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邢福武，李泽贤，叶华谷，陈炳辉，吴德邻ＡＳＴＵＤＹＯＮＴＨＥＦＬＯＲＩＳＴＩＣＰＬＡＮＴＧＥＯＧＲＡＰＨＹＯＦＸＩＳＨＡＩＳＬＡＮＤＳ，ＳＯＵＴＨＣＨＩＮＡ￥ＸｉｎｇＦｕｗｕ；ＬｉＺｅｘｉａｎ；ＹｅＨｕａｇｕ；ＣｈｅｎＢｉｎｇｈｕｉ；ＷｕＤｅｌｉｎ（... 相似文献
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YU Shanshan KONG Xiaoyu LI Yulong XU Hui 《中国海洋大学学报(英文版)》2007,6(2):187-192
The complete mitochondrial cytochrome oxidase subunit Ⅱ （COⅡ） gene of Penaeinae shrimp Fenneropenaeus chinensis was cloned and sequenced. The gene is 688 bp in length and codes for 229 amino acids. It shows 83.2%, 87.0% and 83.8% sequence similarity to Marsupenaeus Japonicus, Penaeus monodon and Farfantepenaeus notialis, respectively. The A＋T content of the whole gene and that at the third position of codons are 64.7% and 78.2%, respectively. The phylogenetic relationship between F. chinensis and three other species representing genera Farfanatepenaeus, Marsupenaeus and Penaeus was analyzed. Results showed that the genetic distances among the four taxa ranged from 0.144 0 to 0.200 5, exceeding those estimated with COⅠ and partial 16S rRNA gene sequences among Marsupenaeus, Litopenaeus and Melicertus, and being therefore larger than the value among subgenera. It has been suggested that the COⅡ gene has a faster evolutionary rate than that of the COⅠ gene and partial 16S rRNA gene and could be used for phylogenetic analysis at genus or species level. The results of the present study indicated that Farfantepenaeus, Fenneropenaeus, Marsupenaeus and Penaeus are at a higher phylogenetic level than subgenus, which supports the opinion of the elevation of phylogenetic status of the four subgenera to genus level. 相似文献
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Two new species of the asteroid family Ophidiasteridae are described from two specimens collected by commercial fishery vessels
of Qingdao from the vicinity of Diaoyudao (25°45′N, 123°15′E), East China Sea.Hacelia tuberculata sp. nov. shows affinities withH. tyloplax (H. L. Clark, 1914) andLinckia gracilis sp. nov. is related toL. laevigata (Linnaeus).
Contribution No. 1163 from the Institute of Oceanology, Academia Sinica. 相似文献
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An oligopeptide permease A(OppA)was purified from the extracellular product of Vibrio harveyi SF-1.The molecular weight of the purified protein was estimated to be 58 kDa on SDS-PAGE.The purified protein showed phospholipase C activity at the optimal values of temperature 50℃ and pH 8.0.The enzymatic activity decreased when the temperature increased to 40℃.The N-terminal sequence of the purified protein was determined as ADVPAGTKLA,which is similar to that of OppA.The OppA pre-cursor gene was cloned from th... 相似文献