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高效液相色谱-离子阱质谱法测定海洋微藻藻粉中的8种脂溶性毒素 总被引:1,自引:0,他引:1
建立了利用高效液相色谱-电喷雾离子阱质谱(HPLC-ESI-IT-MS)测定海洋微藻藻粉中8种典型脂溶性毒素的分析方法。藻粉样品经超声细胞破碎后,采用超声波辅助提取法对藻毒素进行提取,用HPLC-ESI-IT-MS多反应离子监测(MRM)模式对各种毒素(包括大田软海绵酸(OA)、鳍藻毒素1(DTX-1)、扇贝毒素2(PTX-2)、虾夷扇贝毒素(YTX)、原多甲藻酸1(AZA1)原多甲藻酸2(AZA2)、罗环内酯毒素(SPX),米氏裸甲藻毒素(GYM))进行测定。8种脂溶性藻毒素均在线性范围内线性关系良好(R2均在0.991以上),检出限均介于0.085~1.315 pg之间,加标回收率在88.5%~111.4%之间,方法重复性相对标准偏差(RSD)在4.82%~10.17%范围。应用该方法对利玛原甲藻干藻粉中的毒素进行了测定,分析结果良好,说明本方法是海洋微藻藻粉中脂溶性藻毒素测定的有效方法。 相似文献
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Herbaceous plants are widely distributed on islands and where they exhibit spatial heterogeneity. Accurately identifying the impact factors that drive spatial heterogeneity can reveal typical island biodiversity patterns. Five southern islands in the Miaodao Archipelago, North China were studied herein. The spatial distribution of herbaceous plant diversity on these islands was analyzed, and the impact factors and their degree of impact on spatial heterogeneity were identified using CCA ordination and ANOVA. The results reveal 114 herbaceous plant species, belonging to 94 genera from 34 families in the 50 plots sampled. The total species numbers on different islands were significantly positively correlated with island area, and the average α diversity was correlated with human activities, while the β diversity among islands was more affected by island area than mutual distances. Spatial heterogeneity within islands indicated that the diversities were generally high in areas with higher altitude, slope, total nitrogen, total carbon, and canopy density, and lower moisture content, pH, total phosphorus, total potassium, and aspect. Among the environmental factors, pH, canopy density, total K, total P, moisture content, altitude, and slope had significant gross effects, but only canopy density exhibited a significant net effect. Terrain affected diversity by restricting plantation, plantation in turn influenced soil properties and the two together affected diversity. Therefore, plantation was ultimately the fundamental driving factor for spatial heterogeneity in herbaceous plant diversity on the five islands. 相似文献
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近十年来渤海湾围填海工程对渤海湾水交换的影响 总被引:2,自引:0,他引:2
近十年来我国海岸带地区快速城市化,对土地的需求持续高涨,导致沿海地区围海造地现象愈演愈烈,海湾纳潮海域面积持续萎缩,对海湾的水交换带来了较大压力。为此,选取我国海岸带快速城市化的典型区域——渤海湾为例,研究近十年来渤海湾海岸带城市化对渤海湾水交换的影响。基于国际流行的MIKE3水动力模型耦合粒子追踪模块,应用2000年和2010年两个典型年份的渤海湾岸线资料,分别计算了2000年和2010年渤海湾环流系统及其驱动下的水质点运移规律和变化特征。研究将渤海湾划分为8个区块,定量化研究和对比了近十年来由于渤海湾持续围填海导致的海湾水存留时间和不同区块水交换能力的变化。结果表明,随着近十年来渤海湾沿岸快速城市化产生的岸线变化,导致湾内环流涡旋增多,决定湾内水交换的流场结构发生了显著变化,导致各区块的水交换能力和平均水存留时间不仅存在较大差异,而且发生了显著的变化。与2000年相比,2010年天津港、黄骅港和曹妃甸港区海域的平均水存留时间明显增加,水交换率下降,对区域水质的改善十分不利。 相似文献
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近岸海域的生态系统管理,要求对海洋健康进行综合的评估以及对人工调控进行科学的指导,进而促进可持续发展。本文构建了定量化方法,用于评估当前的和近期的基于生态系统服务的海洋健康。以莱州湾为例的研究结果表明,当前的海洋健康指数分值为0.7856(总分为1.0);通过对负面压力进行不同强度的人工调控,得到近期海洋健康预计的变化范围为0.5551到0.8041。具体地说,本文评估得到当前的海洋健康主要表现为文化服务和供给服务基本优秀,而支持服务和调节服务不够良好。若不对持续增长的压力采取有效的调控措施,该近岸海域生态系统会在近期丧失部分支持服务和调节服务;若负面压力被完全控制,该近岸海域生态系统服务的分类等级会得到小幅提升。另外,应进一步加强对生态系统服务尤其是文化服务和调节服务的宣传教育。本文的分析过程和量化结果,为进一步指导人工调控提供了灵活的工具,有助于海岸带区域的生态系统管理。 相似文献
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我国正处于工业化、城镇化进程加快的时期,能源消费强度较高。随着经济规模进一步扩大,我国能源需求持续增长对能源供给形成很大压力,石油天然气对外依存度将进一步提高。我国石油天然气资源相对不足,但能源勘探技术相对落后加剧了能源供给能力的提高,不少国际石油公司借助技术优势以合作开发的形式参与我国油气资源开发。虽然海洋油气资源的合作开发模式表面看起来实现了双方的共赢,但海洋油气资源的合作开发具有诸多潜在的危害性。 相似文献
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Prediction and sensitivity models,to elucidate the response of phytoplankton biomass to environmental factors in Quanzhou Bay,Fujian,China,were developed using a back propagation(BP) network.The environmental indicators of coastal phytoplankton biomass were determined and monitoring data for the bay from 2008 was used to train,test and build a three-layer BP artificial neural network with multi-input and single-output.Ten water quality parameters were used to forecast phytoplankton biomass(measured as chlorophyll-a concentration).Correlation coefficient between biomass values predicted by the model and those observed was 0.964,whilst the average relative error of the network was-3.46% and average absolute error was 10.53%.The model thus has high level of accuracy and is suitable for analysis of the influence of aquatic environmental factors on phytoplankton biomass.A global sensitivity analysis was performed to determine the influence of different environmental indicators on phytoplankton biomass.Indicators were classified according to the sensitivity of response and its risk degree.The results indicate that the parameters most relevant to phytoplankton biomass are estuary-related and include pH,sea surface temperature,sea surface salinity,chemical oxygen demand and ammonium. 相似文献
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