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采用组化方法首次探讨碱性磷酸酶在文昌鱼体内的分布。结果发现 :碱性磷酸酶存在于腹褶部分表皮、围腮腔上皮、构成背鳍腔的内皮、肌膜、中肠腔和肝盲囊腔细胞纹状缘、生殖上皮和精原细胞中 相似文献
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对青岛文昌鱼卵巢和卵母细胞的发育作了周期性的观察。结果表明,青岛文昌鱼的卵巢和卵的发育,在繁殖季节前并不同步,早在第一年的12月就有卵黄生成期晚期的卵母细胞,而在第二年的6—7月达到成熟、产卵。文章还提出在沙子口的青岛文昌鱼可能有着季节性迁移的习性。 相似文献
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ＵｌｔｒａｓｔｒｕｃｔｕｒａｌｓｔｕｄｙｏｆｏｏｇｅｎｅｓｉｓｉｎＸｉａｍｅｎａｍｐｈｉｏｘｕｓ￥ＦａｎｇＹｏｎｇｑｉａｎｇａｎｄＱｉＸｉａｎｇ（ＲｅｃｅｉｖｅｄＡｐｒｉｌ１０，１９９３；ａｃｃｅｐｔｅｄＪｕｌｙ９，１９９３）Ａｂｓｔｒａｃｔ：─—Ｕ... 相似文献
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于1997年6月在青岛市沙子口海域采集文昌鱼样品，采用RT－PCR方法进行青岛文昌鱼LIM类同源框基因片段的扩增研究。结果表明，用一对引物一次PCR扩增的方法未能得到特异的PCR产物，用一对引物两次PCR扩增的方法也同样未果。在对套式PCR进行研究时发现，用普遍PCR方法进行的套式PCR结果不够理想，而用三步两段法和四步两段法套式PCR则可进行成功的扩增。用这两种方法扩增的PCR产物具有高度特异性。相比之下，用简并的引物对未知的基因片段进行PCR扩增时，三步两段法和四步两段法的套式PCR应是首选方法。 相似文献
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In order to realize the spatiotemporal variations of benthic macrofaunal communities at the "Amphioxus Sand"habitat, six surveys including four seasons and three consecutive summers(i.e., 2014, 2015 and 2016) were conducted in two core sites, i.e., Huangcuo(HC) and Nanxian-Shibaxian(NX), in the Xiamen Amphioxus Nature Reserve in China. A total of 155 species of macrofauna were recorded, therein, polychaetes were dominant in terms of species number and density. Significant spatiotemporal variations of macrofaunal communities were observed. The density of polychaetes and the biomass of molluscs in the HC site were higher than those in the NX site. Macrofauna were more diverse and abundant in the cold seasons(winter and spring) than that in the warm seasons(summer and autumn). The annual variations of macrofaunal communities may be attributed to the changes in sediment texture among the three years of the survey. The variations in macrofaunal communities were mainly related to the proportion of polychaetes within the community. In addition, the density of amphioxus(include Branchiostoma japonicum and B. belcheri) was negatively correlated to that of polychaetes, bivalves, and crustaceans. Amphioxus was less likely to be found in the sediments with higher silt and clay content. Five biotic indices including Margaref's richness index(d), Peilou's evenness index(J′), Shannon-Wiener diversity index(H′),AMBI and M-AMBI were calculated in the present study. AMBI seems suitable in assessing benthic health at the"Amphioxus Sand" habitat, and a potential risk of ecological health in Xiamen Amphioxus Nature Reserve should be aware. 相似文献
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在繁殖季节，给正在成熟的文昌鱼体腔注射ＧｎＲＨ－Ａ，结果表明，注射激素后３６ｈ时，有５０％文昌鱼产卵，未产动物成熟卵母细胞达９５．５％。而对照组无任何动物产卵，成熟卵母细胞为５５％，我们由引首次直接证实文昌鱼脊椎动物一样，ＧｎＲＨ－Ａ是调控其卵母细胞成熟和产卵的重要激素。 相似文献
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文昌鱼幼体体腔、血管发育的初步研究 总被引：2，自引：0，他引：2
本文研究了24,48h文昌鱼幼体体腔、血管的发育状况,旨在为研究文昌鱼体腔细胞、粒细胞的起源和分化提供依据。1 材料和方法1.1 实验材料青岛文昌鱼(BranchiostomabelcheriTsingtauense)24,48h幼体。1.2 方法文昌鱼幼体经2.5%戊二醛(0.1mol/L二甲砷酸钠配制,pH7.3)固定1h,1%锇酸固定1h,然后经系列酒精脱水,Epon812包埋。半薄切片(2μm)经碱性美蓝-品红染色[1],光镜观察、照相。半薄切片定位的样品经超薄切片,醋酸双氧铀、柠檬酸铅染色,JEM-100cx电镜观察、照相。2 结果2.1 光镜观察结果青岛文昌鱼卵子在室温条件下自受精后50～60min… 相似文献
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