Ontogeny of the lymphoid organs of Japanese flounder, Paralichthys olivaceus     

Liu Yun  Jiang Guoliang  Zhang Shicui 《中国海洋大学学报(英文版)》2004,3(2):161-165

Histological study on the ontogeny of the lymphoid organs, kidney, thymus and spleen of Japanese flounder, Paralichthys olivaceus, from hatching to 40 d was carried out. The pronephric kidney duct appeared early in hatching although the primordial haemopoietic stem cells were observed within a week after hatching. The spleen was first seen after 8d of hatching. The thymus appeared after 15d, situated near the pronephric kidney. Small lymphoid cells appeared during the later phase of the post-larval stage in the sequence of thymus, kidney and spleen. During the 40d of observations, there were no distinct inner or outer zones in thymus and no red or white pulp in spleen. These results suggest that the nonspecific defense immune system plays a very important role in the early larval stage of Japanese flounder.  相似文献   

CHARACTERIZATION OF LACTATE DEHYDROGENASE ISOZYME PATTERN AND MORPHOLOGY OF THREE MARINE FISH CELL LINES     

郭华荣  张士璀  李红岩  童裳亮  相建海 《中国海洋湖沼学报》2002,20(3):226-231

Three continuous marine fish cell lines of FG (i. e., Hounder Gill) from flounder (Paralichthys olivaceus) gill, SPH (i. e. , Sea Perch Heart) from sea perch (Lateolabrax japonicus) heart and RSBF (i.e., Red Sea Bream Fin) from red sea bream (Pagrosomus major) fin, were characterized by lactate dehydrogenase (LDH) isozyme and morphological analysis. The LDH isozyme patterns of these three cell lines and their corresponding tissues of origin were investigated and compared. The results showed: (1) No difference was found in the LDH isozyme patterns of FG and flounder gill tissue. However, the LDH isozyme patterns of SPH and RSBF were significantly different from their corresponding tissues of origin; (2) LDH isozyme patterns of FG, SPH and RSBF were markedly different from each other and could serve as genetic markers for species identification and detection of cross contamination. Morphological change analysis of these three cell lines in comparison to their original tissues indicated that FG cells still appeared epithelioid without morphological transformation. However, morphological changes were found in SPH and RSBF compared to their original tissues. Therefore, the cellular morphology was still plastic in the relatively stable culture conditions, and it was possible that change of LDH patterns wasrelated to morphological changes of fish cells in vitro.  相似文献   

杂交鲆(牙鲆♀×夏鲆♂)胚胎发育的初步观察     

于道德  肖志忠  徐世宏  马道远  李军 《海洋科学》2007,31(2):55-60

通过人工调控促使亲本牙鲆(Paralichthys olivaceus)♀×夏鲆(Paralichthys dentatus)♂的性腺发育,人工干法授精获得杂交受精卵。杂交受精卵在温度为16.5~17.5℃,光照为100~400 lx,微充气条件下孵化。对杂交鲆胚胎发育全过程进行连续观察并与牙鲆及夏鲆的胚胎发育进行比较。结果表明:杂交鲆卵裂方式与其他大部分硬骨鱼类一样,属于盘状卵裂。受精后经过2 h 30 min进入2细胞期,7 h 45 min进入囊胚期,15 h 10 min进入原肠胚阶段,25 h 30 min进入神经胚期,49 h左右进入尾芽期。授精后63 h左右发现初孵仔鱼,68h左右50%的仔鱼孵出。在杂交鲆的胚胎发育中,主要有2个发育特征与母本类似而与父本不同:视囊的出现要早于体节的分化;孵化前,仔鱼在卵膜内环绕卵黄囊不到1周。但是在其孵化时间上却与父本相似。  相似文献   

海水温度和盐度对泥蚶幼虫和稚贝生长及存活的影响   总被引：24，自引：0，他引：24  

尤仲杰  徐善良  边平江  陈坚 《海洋学报》2001,23(6):108-113

1996～1997和1999年在浙江省乐清市东发水产育苗场和福建省宁德市四海水产育苗场分别研究了海水温度和盐度对泥蚶浮游幼虫和稚贝生长及存活的影响,结果表明,泥蚶浮游幼虫的适宜温度为25～33℃,最适生长温度为28～30℃.稚贝的适宜温度为15～35℃,最适生长温度为25～30℃.浮游幼虫的适宜盐度为16.54～30.02,最适生长盐度为1654～23.38;稚贝的适宜盐度为10.01～30.02,最适生长盐度为10.01～23.38.泥蚶幼虫和稚贝对高温和低盐海水有较强的适应能力,这与它们夏季的繁殖期和自然分布于内湾河口区是相适应的.  相似文献   

利用牙鲆鳃细胞系分离和培养淋巴囊肿病毒   总被引：1，自引：4，他引：1  

吕宏旭  汪岷  李红岩  郭华荣  张士璀  包振民  周丽 《中国海洋大学学报(自然科学版)》2003,33(2):233-239

本文利用牙鲆鳃细胞系进行了养殖牙鲆淋巴囊肿病毒的分离及培养 ,并通过电镜对培养细胞中淋巴囊肿病毒的形态及感染循环进行了初步研究。将病鱼的淋巴囊肿组织无菌滤液接种牙鲆细胞系 ,细胞出现了明显的细胞病变 ( Cytopathic effect,CPE)。电镜观察在培养细胞的胞质中有病毒的包涵体 ,胞质中散在 6角形、5角形或圆形的病毒粒子 ,大小为 10 0～ 140 nm之间。在感染细胞的线粒体中也存在大量的病毒颗粒。  相似文献   

Metazoan parasites of flounder Platichthys flesus (L.) along a transect from the southwestern to the northeastern Baltic Sea     

Koie  M. 《ICES Journal of Marine Science》1999,56(2):157-163

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两项新技术及其在海水苗种生产中的应用   总被引：4，自引：0，他引：4  

杜守恩 《海洋湖沼通报》1995,(3):59-62

本文介绍了两项新技术，用泡沫净化空气、海水和生物效应灯，及其在海水苗种生产中的应用。文中分别讨论了它们的原理、设计安装及使用效果。希望这两项新技术能够得到推广，以提高出苗率，增加海水苗种生产的经济效益。  相似文献   

石鲽鱼人工育苗技术研究   总被引：1，自引：0，他引：1  

王立超  刘增华  王远洋  高永刚  连岩  张志新  倪高田 《海洋科学进展》2001,19(1):55-59

根据石鲽鱼的生物学特性 ,对亲鱼的选择培育、人工催产、挤卵、受精孵化和仔稚鱼的培育技术 ,进行试验探索。在室内人工控制水温、光照、饵料投喂的条件下 ,较为系统地研究了石鲽鱼的发育和变态及其与理化因子的关系 ,探索了人工育苗的关键技术 ,突破了过去单纯依赖采捕自然苗进行人工养殖的方式 ,为实现室内全人工育苗及养成产业化生产 ,解决苗种来源途径提供了技术保证  相似文献   

The combined effect of transport and temperature on distribution and growth of larvae and pelagic juveniles of Arcto-Norwegian cod   总被引：5，自引：0，他引：5  

Vikebo  Frode; Sundby  Svein; Adlandsvik  Bjorn; Fiksen  Oyvind 《ICES Journal of Marine Science》2005,62(7):1375-1386

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几种大型珍珠贝人工苗网箱式中间培育的研究   总被引：2，自引：0，他引：2  

梁飞龙  邓陈茂  符韶  黄海立  周银环  刘永 《海洋湖沼通报》2008,(3)

采用网箱式和传统式2种方法,在3个不同类型的海区(内湾性的陵水县黎安港、半开放性的雷州市流沙港、开放性的三亚市六道湾),进行几种大型珍珠贝人工苗的中间培育.经90d的中间培育后,结果表明:网箱式培育与传统式培育相比较,几种珍珠贝的生长速率和成活率都有较大幅度的提高.其壳高生长速率的增幅分别为:珠母贝18.9%、18.2%、19.9%;大珠母贝22.8%、20.6%、23.5%;企鹅珍珠贝21.8%、20.7%、21.7%;均为差异显著(P＜0.05)或差异极显著(P＜0.01).成活率的增幅分别为:珠母贝175.0%、147.9%、133.9%;大珠母贝172.8%、159.0%、127.4%;企鹅珍珠贝122.5%、118.2%、102.2%,均为差异显著(P＜0.05)或差异极显著(P＜ 0.01).  相似文献