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本文从水产无脊椎动物与环境的关系论述其来克丁物质的功能;来克丁与微生物间的相互作用包括相互作用中有关来克丁、微生物种类,作用部位和作用物质等。从来克丁在水产无脊椎动物识别外来物质,包括抵御病原体的作用看,健康发展的水产业之一项关键任务便是认识和掌握来克丁在御防病害活动中的机理。 相似文献
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Tagging indicates at least two stocks of snapper Chrysophrys auratus in south‐east australian waters
M. J. Sanders 《新西兰海洋与淡水研究杂志》2013,47(2):371-374
During 1957 to 1962, 4 155 snapper Chrysophrys auratus (Bloch & Schneider) were tagged and released at a number of sites in Victoria. From 1957 until 31 June 1972, 210 of these fish have been reported, and the relationship between the release and recapture sites indicates the existence of at least two stocks: One stock extends from central Victoria to eastern South Australia, and the other extends from eastern Victoria to northern New South Wales. 相似文献
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C-型凝集素(C-typelectin,CTL)是甲壳类动物体液免疫中重要的免疫因子之一。但CTL基因在溶藻弧菌对三疣梭子蟹(Portunustrituberculatus)感染的过程中的表达及抗病机制尚有待进一步研究。为初步阐明CTL基因SNP E4-205 C/T位点与抗溶藻弧菌感染相关的分子机制,对不同基因型梭子蟹进行溶藻弧菌感染,通过绝对定量方法分析不同基因型梭子蟹肝胰腺、肌肉组织中溶藻弧菌的复制情况。发现溶藻弧菌感染后12 h内梭子蟹肝胰腺、肌肉组织中C/C组细菌数量显著高于T/T组,结果表明T/T组梭子蟹抗感染能力显著高于C/C组。进一步对该位点非同义突变(ACT-ATT)导致的一个氨基酸改变(Thr-Ile)的两种蛋白进行体外重组表达,并对两种重组蛋白CTL-ATT及CTL-ACT进行活性分析。发现两种重组蛋白对溶藻弧菌生长均具有一定的抑制作用, CTL-ATT的抑菌活性显著高于CTL-ACT。重组蛋白CTL-ATT与PAMPs的结合活性高于CTL-ACT与PAMPs的结合活性,同时两种蛋白与PAMPs和溶藻弧菌的结合活性具有浓度依赖性。结果表明三疣梭子蟹CTL基因参与机... 相似文献
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泥蚶凝集素的抑菌作用和免疫学研究 总被引：1，自引：0，他引：1
分别从泥蚶血淋巴液和肌肉中提取出凝集素,采用全血微量培养法进行淋巴细胞转化和抑菌实验。结果表明:两种凝集素都具有促进小鼠淋巴细胞转化的免疫调节功能;两种凝集素对供试的金黄色葡萄球菌(Staphylococcus aureus),甘薯薯瘟病原细菌(Fasarium oxyssporam),大肠杆菌(Escherichia coli),普通变形杆菌(Proteas valgaris),八叠球菌(Sarcina lutea)5种细菌和啤酒酵母(Saccharomyces carlsbergensis),玉米大斑病菌(Helminthos porium turcicum),稻瘟病菌(Piricularia oryzae)3种真菌都有不同程度的抑制作用,且对真菌的抑制作用大于对细菌的抑制作用;由二者分别制备的抗血清通过免疫双扩散法并不能与对方发生交叉沉淀反应,说明它们不具有同源性。 相似文献
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戴聪杰 《海洋通报(英文版)》2005,7(1):77-81
Twelve species of crustacean from Fujian coast were examined for lectins with different animal erythrocytes. Serum extracts fromall of 12 species showed agglutinating activity against at least two types of the erythrocytes used, which revealed the existence of lectins in these species. There were five species ( Penaeus japonicus, Lophosquilla costata, Charybdis feriatus, Portunus pelagicus, Scylla serrata ) whose serums could agglutinate all the erythrocytes tested. The lowest serum protein concentration required to produce erythrocytes agglutination varied remarkably, ranging from 0.7 μg/mL to 8 080 μg/mL. The strongest activity was shown in the agglutination of rabbit erythrocyte by serum of Penaeus vanaminas. Inhibition assays performed with seven mono- and bisaccharides showed that agglutination of quail erythrocytes by serums of three species (Portunus pelagicus, Scylla serrata and Sesarma sp. ) were not inhibited by any sugars, while others were inhibited by at least three types of sugars. The assay of temperature influence on agglutinating activity showed that only Penaeusjaponicus retained activity when the serum was heated to 90℃, and other serums lost their activity at 40 ℃, 50 ℃, 60 ℃, 70 ℃, 80 ℃ for 10 minutes, respectively. 相似文献
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FITC‐conjugated lectins proved to be effective probes for differentiating between morphologically similar dinoflagellate species isolated from New Zealand coastal waters. In particular the binding (fluorescence) of peanut (PNA) lectin differentiated G. mikimotoi from Gymnodinium sp. (Waimangu) and G. pulchellum and the non‐binding of Helix pomatia (HPA) and wheat germ (WGA) lectins discriminated between G. mikimotoi and the other Gymnodinium species tested. G. breve (Florida) was differentiated from the New Zealand isolates by binding with soy bean (SBA) lectin. Ulex europeus (UEA) distinguished the toxic species Alexandrium minutum from the morphologically similar, but non‐toxic, Cachonina hallii. Two strains of Prorocentrum lima (Spain and Rangaunu) were not differentiated by the lectins, but P. lima was differentiated from P. compressum. 相似文献
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利用血凝试验及糖抑制试验测定脊条褶虾蛄的血清及肌肉提取液的凝血活性及糖抑制专一性，同时进行热稳定性、pH及Ca^2 影响试验。结果显示：其血清能凝集12种红细胞，且其凝集效价高于肌肉提取液（兔、蛇、鲫鱼除外）。血清对鹌鹑红细胞的凝集可被D－果糖、D－半乳糖和蔗糖所抑制，最小抑制浓度均为200mmol/dm^3，其凝集活性均依赖于Ca^2 ，在pH值为5.0-9.0范围内较稳定，经40℃保温10min后活性完全消失；肌肉提取液不被7种糖抑制，在pH值为4.0-10.0、温度为40－90℃范围内均具活性，说明有很强的耐热性，其凝集性不依赖于Ca^2 。以上结果表明：脊条褶虾蛄血清与肌肉提取液均含有凝集素，且凝集素的种类不同。 相似文献
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为寻找凝集素作为分子探针提供基础资料,用12种甲壳动物血清抽提液对天然的或经酶修饰的鹌鹑红细胞进行凝集试验。实验结果表明,经蜗牛酶修饰后的鹌鹑红细胞的凝集作用发生了变化,与未经酶处理的鹌鹑红细胞相比,凝集活性提高的有日本沼虾（Macrobrachium nipponense）等7种,凝集活性下降的有罗氏沼虾（Macrobrachium rosenbergi）等2种。凝集素对动物红细胞的凝集可看作是与这些细胞表面的多糖或糖蛋白——即受体的识别性鲒合,这表明鹌鹑红细胞表面具有12种凝集素的受体,且大多数受体都受到酶修饰的影响,从而在凝集活性上表现出差异。 相似文献
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