强光照和黑暗条件下刺参体壁的基因表达差异     

张立斌  冯其明  孙丽娜  房燕  徐冬雪  张涛  杨红生 《海洋学报(英文版)》2018,37(5):54-66

参对光照变化非常敏感，研究刺参对光照的分子响应非常重要。本研究应用RNA测序获取了刺参暴露于强光（“强光”）、正常光照（“对照”）和完全黑暗（“黑暗”）环境下体壁的基因表达谱情况，通过“对照”与“黑暗”，“对照”与“强光”和“黑暗”与“强光”的比较，在|log₂ ratio|≥1和FDR≤0.001的标准下分别发现了1161、113和1705个差异表达基因（DEGs）。基因本体分析表明，“cellular process”和“binding”在“生物过程”和“分子功能”类别中的DEGs富集最多，而“cell”和“cell part”在“细胞组分”类别中的DEGs富集最多。将DEGs与Kyoto Encyclopedia基因和基因组数据库上的于214、41和229条通路进行比对，发现了51、2和57条通路分别显著富集。本研究发现的光特异性DEGs可作为深入研究刺参对光照变化的生化适应机制的重要目标基因。  相似文献   

Revisiting the existence of an effective stress for wet granular soils with micromechanics          下载免费PDF全文

Jérôme Duriez  Richard Wan  Mehdi Pouragha  Félix Darve 《国际地质力学数值与分析法杂志》2018,42(8):959-978

A possible effective stress variable for wet granular materials is numerically investigated based on an adapted discrete element method (DEM) model for an ideal three‐phase system. The DEM simulations consider granular materials made of nearly monodisperse spherical particles, in the pendular regime with the pore fluid mixture consisting of distinct water menisci bridging particle pairs. The contact force‐related stress contribution to the total stresses is isolated and tested as the effective stress candidate for dense or loose systems. It is first recalled that this contact stress tensor is indeed an adequate effective stress that describes stress limit states of wet samples with the same Mohr‐Coulomb criterion associated with their dry counterparts. As for constitutive relationships, it is demonstrated that the contact stress tensor used in conjunction with dry constitutive relations does describe the strains of wet samples during an initial strain regime but not beyond. Outside this so‐called quasi‐static strain regime, whose extent is much greater for dense than loose materials, dramatic changes in the contact network prevent macroscale contact stress‐strain relationships to apply in the same manner to dry and unsaturated conditions. The presented numerical results also reveal unexpected constitutive bifurcations for the loose material, related to stick‐slip macrobehavior.  相似文献   

基于灯光数据的京津冀城市多标度异速分析     

龙玉清  陈彦光 《地理科学进展》2019,38(1):88-100

城镇体系的时空演化是一个复杂的动力学过程,具有无尺度性,其特征可以采用异速标度指数定量描述。论文以京津冀为例,基于1992—2013年夜间灯光数据的校正结果,提取城镇夜间灯光信息,采用多标度异速分析方法,分析京津冀城镇体系的相对发展特征及其空间分异格局。研究发现：①京津冀大城市的增长空间局限日益显著。虽然大城市如京津唐的绝对发展水平高,但接近环境承载量的极限,22 a间增长快速的是大城市交接地带的小城市(如三河、迁安、廊坊等)。②相对增长速度快的城市从东北到西南呈现带状分布——政府选择发展的“雄安新区”就在这个轴带上。由此可以得出两个结论：其一,京津冀城市演化的趋势相对均衡化,空间结构异质性逐步减弱,整个城镇体系的发展趋于相对平衡状态;其二,大、小城市“两极”贯通或许是增强城镇体系功能的有效途径。京津冀的空间格局优化不能仅仅考虑主要的、规模大的中心城市,而应该基于等级结构协调整个城镇网络体系。  相似文献   

全球气候变化下南海诸岛保护优先区识别分析   总被引：3，自引：1，他引：2  

左秀玲  苏奋振  张宇  吴文周  吴迪 《地理学报》2020,75(3):647-661

全球变化下,珊瑚礁保护区是保护生物多样性、增强珊瑚礁对气候变暖抵抗力的有效方式,而维持珊瑚礁弹性是其核心内容。针对珊瑚礁最具有威胁性的热压力因子,基于南海1982—2009年卫星观测海表面温度（SST）数据和CMIP5加拿大地球系统模式CanESM2模型预估的2006—2100年南海SST数据构建热压力强度模型,从维持珊瑚礁弹性的角度识别IPCC RCP 4.5和RCP 8.5情景下南海诸岛保护优先区。结果表明：RCP 4.5和RCP 8.5情景下13%左右的南海诸岛珊瑚礁识别为保护优先区。根据热压力强度与珊瑚抵抗力及避难所关系,西沙群岛七连屿和晋卿岛近年观测与未来预估的热压力强度均比较低,在保障其服务功能的基础上建议实施完全保护;东沙群岛东沙环礁和中沙环礁排洪滩近年观测急性热压力强度较高但未来预估热压力强度较低,建议实施50%禁止利用保护;中沙群岛黄岩岛近年观测和未来预估的急性热压力强度均比较低,建议实施50%多用途保护。南沙群岛有14%左右的珊瑚礁识别为保护优先区,根据其热压力强度可实施30%~100%禁止利用保护或30%~50%多用途保护。RCP 4.5和RCP 8.5情景下的南海诸岛保护优先区及保护对策,可为维持珊瑚礁生态弹性及应对全球气候变化提供重要的参考价值。  相似文献   

光照强度和光色对番红砗磲(Tridacna crocea)氨氮、活性磷酸盐及氧代谢的影响     

刘春胜  刘小霞  汪浩  王爱民  顾志峰 《海洋与湖沼》2018,49(2):313-318

采用室内实验生态学方法,以水体中氨氮、活性磷酸盐和溶氧变化为指标研究了光照强度和光色对番红砗磲代谢的影响。结果表明:(1)在0—10000lx光照时,番红砗磲排氨量逐渐减少,在光照12000lx时转为吸收海水中氨氮,并在16000lx时达到最高[1.89μg/(ind·h)];在2000lx光照条件下,番红砗磲可吸收海水中活性磷酸盐并释放氧气。其活性磷酸盐吸收量随光照强度增强而增加,在16000lx时达到最高峰。在实验光照范围内,番红砗磲产氧率随光照强度的增强逐渐增加。上述结果显示番红砗磲最佳光照强度约为16000lx。(2)当番红砗磲自黑暗转移至各光照组后,其氨氮、活性磷酸盐吸收率均随时间逐渐降低,而产氧率则逐渐升高。(3)光色显著影响番红砗磲合成代谢,蓝光最佳,红光次之,白光最差。  相似文献   

The Upper Devonian Kellwasser event recorded in a regressive sequence from inner shelf to lagoonal pond,Catalan Coastal Ranges,Spain     

《Sedimentology》2018,65(6):2055-2087

This study, conducted in the Catalan Coastal Ranges, north‐east Spain, describes the Upper Devonian Kellwasser event in a shallowing‐upward sequence of black shales, siltstones and quartz arenites. This sequence was deposited in a progradational and regressive coastal system where the sedimentary environment evolved from the inner shelf to a lagoonal pond located landward of the shoreline. Three anomalous succeeding steps have been identified by geochemical analysis. The first one, detected on the inner shelf, was characterized by oxygen depletion and high organic productivity. The second, detected in the nearshore, was caused by hydrothermal activity occurring under normal oxic conditions. The third and most intense step was identified in the muds of the lagoonal pond and has been linked to strong anoxic conditions, elevated clastic input derived from changes in the weathering regime at the source area and moderate hydrothermal activity. The Kellwasser event is thus defined in the study area as stepwise and multi‐causal. This is the first time that the Kellwasser event has been identified in a sedimentary environment behind the shoreline. It is also the first time that it has been reported in the Catalan Coastal Ranges.  相似文献   

EFFECTS OF FISH OIL,DHA OIL AND LECITIHIN IN MICROPARTICULATE DIETS ON STRESS TOLERANCE OF LARVAL GILTHEAD SEABREAM（SPARUS AURATA）     

刘镜恪王文琪  李岿然雷霁霖 《中国海洋湖沼学报》2002,20(4):338-343

The effects of natural fish oil,DHA oil and soybean lecithin in microparticulate diets on stress tolerance of larval gilthead seabream(Sparus aurata)were investigated after 15 days feeding trials.The tolerance of larval gilthead seabream to various stress factors such as exposure to air(lack of dissolved oxygen),changes in water temperature(low)and salinity(high) were determined.This study showed that microparticulate diet with natural fish oil and soybean lecithin was the most effective for in-creasing the tolerance of larval gilthead seabream to various stresses,and that microparticulate diet with natural fish oil and palmitic acid(16:0)was more effective than microparticulate diet with DHA oil and soybean lecithin.  相似文献   

Heat-shock protein 70 expression in shrimp Fenneropenaeus chinensis during thermal and immune-challenged stress   总被引：4，自引：0，他引：4  

郭振宇焦传珍  相建海 《中国海洋湖沼学报》2004,22(4):386-391

Using western immunoblotting, we obtained heat-shock protein 70 (HSP70) induction data and distribution in different tissues from shrimp Fenneropenaeus chinensis during thermal and immune-challenged stresses. This is probably the first report of the effects of various stressors on the expression of HSP70 in shrimp.HSP70 was prominently induced in hepatopancreas and gills, but not in muscle, eyestalk and hemolymph, when the shrimp were exposed to heat shock and Vibrio anguillavium-challenged stresses. Cold shock and WSSV treatment had no significant effects on the levels of HSP70 expression in all tissues examined. HSP70 induction was greatest after 2 h exposure to heat shock stress, which was elevated after acute heat shock exposure of 10℃ above ambient temperature.  相似文献   

A critical state model for sands dependent on stress and density     

Y.P. Yao  D.A. Sun  T. Luo 《国际地质力学数值与分析法杂志》2004,28(4):323-337

An elastoplastic model for sands is presented in this paper, which can describe stress–strain behaviour dependent on mean effective stress level and void ratio. The main features of the proposed model are: (a) a new state parameter, which is dependent on the initial void ratio and initial mean stress, is proposed and applied to the yield function in order to predict the plastic deformation for very loose sands; and (b) another new state parameter, which is used to determine the peak strength and describe the critical state behaviour of sands during shearing, is proposed in order to predict simply negative/positive dilatancy and the hardening/softening behaviour of medium or dense sands. In addition, the proposed model can also predict the stress–strain behaviour of sands under three-dimensional stress conditions by using a transformed stress tensor instead of ordinary stress tensor. Copyright © 2004 John Wiley & Sons, Ltd.  相似文献   

Plane of elastic non-resisting tension material under foundation structures     

A. Baratta  I. Corbi 《国际地质力学数值与分析法杂志》2004,28(6):531-542

In the frame of 2D-static problems one approaches the problem of elastic-NRT (not-resisting tension) semi-plane loaded on its limit line. This problem is intended to model the stress situation induced in the soil by a foundation structure. The solution, in terms of activated stress field, is searched for in the class of stress fields satisfying equilibrium and admissibility conditions, by applying an energy approach. Copyright © 2004 John Wiley & Sons, Ltd.  相似文献