合浦珠母贝26S蛋白酶体S4,S7亚基基因片段的分离与分析     

姜铁民  张勇  黄洁  谢莉萍  吴移谋  张荣庆 《海洋科学》2003,27(4):28-34

26S蛋白酶体是真核生物中一种具有ATP依赖性的蛋白酶复合体,主要通过泛肽途径选择性降解细胞内与代谢调控、细胞周期有关的功能蛋白及异常蛋白,参与多种细胞活动的调控过程。26S蛋白酶体由具有催化活性的20S亚复合体和一个具有调节作用的19S亚复合体组成,其中19S亚复合体中的ATP酶亚基是调节26S蛋白酶体活性的重要组件。本通过简并引物PCR手段,从软体动物合浦珠母贝(Pinctada fucata)中扩增到参与构成19S亚复合体的S4和S7(MSS1)两个亚基的基因片段。这两个基因片段所编码的ATP酶组件包含有Gx4GKT,DEID,SAT和H／QRxGRxxR等26S蛋白酶体ATP酶亚基的共同功能基序。这是首次在软体动物中报道26S蛋白酶体的ATP酶亚基基因序列,为研究软体动物中26S蛋白酶体的结构与功能奠定了分子基础。  相似文献   

Competition within the marine microalgae over the polar dark period in the Greenland Sea of high Arctic   总被引：2，自引：0，他引：2  

Zhang Qing  Rolf Gradinger  Zhou Qingsong 《海洋学报(英文版)》2003,22(2):233-242

Abstract-With the onset of winter, polar marine microalgae would have faced total darkness for aperiod of up to 6 months. A natural autumn community of Arctic sea ice microalgae was collected fordark survival experiments from the Greenland Sea during the ARKTIS-XI/2 Expedition of RV Po-larstern in October 1995. After a dark period of 161 days, species dominance in the algal assemblagehave changed from initially pennate diatoms to small phytoflagellates (<20μm). Over the entire darkperiod, the mean algal growth rate was-0.01 d~(-1). Nearly all diatom species had negative growthrates, while phytoflagellate abundance increased. Resting spore formation during the dark period was ob-served in less than 4.5% of all cells and only for dinoflagellates and the diatom Chaetoceros spp. We as-sume that facultative heterotrophy and energy storage are the main processes enabling survival during thedark Arctic winter. After an increase in light intensity, microalgal cells reacted with fast growth withindays. Phytoffa  相似文献   

远区台风“三巴”对长江口波浪动力场的作用机制     

任剑波  何青  沈健  徐凡  郭磊城  谢卫明  朱磊 《海洋科学》2020,44(5):12-23

针对远区台风对河口波浪动力场的影响问题,利用第三代波浪模式SWAN计算了远区台风"三巴"期间长江口波浪动力场分布,分析了陆架至河口区的波浪能量耗散和波致泥沙侵蚀的时空分布,发现波浪由外海向近岸传播过程中,波-波相互作用导致能量由高频向低频转换,周期和波长逐渐增大,近底层轨道流速增大,能量密度增高;阐明白帽破碎是维持深水区波浪能量平衡和限制波高成长的主要机制,底摩擦耗能和水深诱导的破碎耗能是长江口横沙东滩和崇明东滩邻近海域波高衰减的主要原因;提出波浪产生的底部切应力与相对水深有关,当波浪传播到浅水区时,波长和周期越大,波浪切应力越大。研究揭示了与河口相距数百公里的远区台风能够对长江口波浪动力场产生明显影响,河口水下三角洲前缘是最容易受到波浪侵蚀的区域,研究成果弥补了目前关于陆架远区台风对河口波浪动力场影响研究的不足,对深化认识远区台风对长江口动力环境、地貌演变、航运安全和滩涂保护等有重要科学意义。  相似文献   

THE CLIMATIC CHANGES AND THE COASTAL ENVIRONMENT     

F. Marabini Qing YunshanA. Veggiani Gang Shuxi 《海洋地质与第四纪地质》1992,(1)

Although the Yellow River Delta and Bohai Sea coastal zone are long distance and different environment with Po River Delta and Adriatic Sea coastal zone, the comparison of data of two different areas and the evolution trend of two areas are quite similar. The influence of natural climatic changes on the evolution of the deltas and coastal zones exists both in ancient and modern times. The cold/wet period occurred in 1300—1400A. D. and 1550 —1850 A.D. within this"large scale" climatie cycle, and shorter periods (10— 35 years) of cold rainy weather alternated with warm/dry period are known as "Bruckner cycles" which have influence on the evolution of the deltas and coastal zones.  相似文献   

Sea ice characteristics between the middle Weddell Sea and the Prydz Bay, Antarctica during the austral summer of 2003   总被引：1，自引：0，他引：1  

TANG Shulin  KANG Jiancheng  ZHOU Shangzhe  LI Zhijun 《海洋学报(英文版)》2005,24(2):9-15

The antarctic sea ice was investigated upon five occasions between January 4 and February 15, 2003. The investigations included: (1) estimation of sea ice distribution by ship-based observations between the middle Weddell Sea and the Prydz Bay; (2) estimation of sea ice distribution by aerial photography in the Prydz Bay; (3) direct measurements of fast ice thickness and snow cover, as well as ice core sampling in Nella Fjord; (4) estimation of melting sea ice distribution near the Zhongshan Station; and (5) observation of sea ice early freeze near the Zhongshan Station. On average, sea ice covered 14.4% of the study area. The highest sea ice concentration (80%) was observed in the Weddell Sea. First-year ice was dominant (99.7%-99.8%). Sea ice distributions in the Prydz Bay were more variable due to complex inshore topography, proximity of the Larsemann Hills, and/or grounded icebergs. The average thickness of landfast ice in NeUa Fjord was 169.5 cm. Wind-blown snow redistribution plays an important role in affecting the ice thickness in Nella Fjord. Preliminary freezing of sea ice near the Zhongshan Station follows the first two phases of the pancake cycle.  相似文献   

TRANSFORMATION OF CONTINENTAL AND OCEANIC CRUSTS AND GEOTECTURE OF THE WESTERN PACIFIC MARGIN     

张文佑  张抗 《海洋学报(英文版)》1987,6(1):94-103

Three types of crust exist in the area studied. There was an assembled continent at the end of the Hercynian-Indosinian cycle. Since the Cenozoic the area has shown a trend of tension, thinning and oceanization in varying degrees, which is manifested in the creeping and obduction of the Asian continent as well as the spreeding and subduction of West Pacific Ocean, thus bringing about the continental rifts, maginal basins and arc-thrence systems in this area.Geotectonic cycle usually begins with a tensional phase and ends with a compressional phase. When tension predominates, various rifts can be formed on the continental crust and the crust is in the trend of oceanization. When compression predominates, the rifts disappears, the crust is in the trend of continentization and, in the end, folded mountain belts and geosutures can be formed. Thus, the crust is tending towards "maturatity" and thickening.  相似文献   

螺旋藻多糖对给予环磷酰胺BALB/C小鼠的保护作用研究   总被引：7，自引：1，他引：7  

王友顺  黎露刚  吴侃  谢宗华  唐庆  倪金星  汪庭  方光如 《海洋科学》1997,21(6):36-39

研究了螺旋藻多糖（ＰＳ）对环磷酰胺（ＣＹ）引起的ＢＡＬＢ／Ｃ小鼠造血功能等损伤的保护作用。当给小鼠ＣＹ时能同时给予ＰＳ时，就能大大减轻因给予ＣＹ经起的造血功能障碍；能明显减缓动物因使用ＣＹ的致的体重下降以及能明显减低因ＣＹ作用的死亡率，提示使用ＰＳ对预防或减轻化疗制剂的危害是具有明显作用的。为临床化疗、放疗病人使用ＰＳ提供了依据。  相似文献   

海洋湍流观测技术   总被引：3，自引：0，他引：3  

康建军  邬海强  杨庆轩  付红丽 《海洋技术学报》2007,26(3):19-23

湍流在海洋能量和水体的交换演化中起着非常重要的作用。海洋湍流研究的发展和海洋湍流观测仪器的研发密切相关。世界上湍流观测仪器的研发起步于上世纪50年代,但是我国在湍流观测设备方面到目前为止仅仅处于起步和引进国外设备阶段。针对我国的情况,文中对海洋湍流观测的平台、探头测量原理及其数据修正、资料后处理等关键技术做一个总结性的介绍,为我国使用引进的海洋湍流设备和自主开发海洋湍流测量设备提供参考。  相似文献   

大黄鱼病原弧菌拮抗放线菌的筛选与人工诱变   总被引：4，自引：0，他引：4  

鄢庆枇  王军  苏永全  梁桂洪  麦添成 《台湾海峡》2002,21(2):187-192

从厦门集美海滩底泥分离得到38株放线菌，用琼脂块法测定它们对大黄鱼病原弧菌-溶藻弧菌和副溶血弧菌的拮抗效果，选择两株拮抗效果较好的放线菌进行诱变，测定诱变后各菌株的抗菌效果，并选择抗菌效果较好的菌株进行第二次诱变，如此反复诱变3次，共得到97株放线菌，结果表明；38株放线菌中的22株对两株病原菌有一定的拮抗作用；用紫外线照射可以获得少量对两株病原菌拮抗作用加强的菌株。  相似文献   

对称式布置锚链系统的线性化处理   总被引：6，自引：2，他引：6  

陈徐均  沈庆等 《海洋工程》2002,20(1):75-79

用悬链线方程求解了不同锚链状态的锚链力 ,并用多项式回归了锚链力与锚固点位移的关系 ,由此求得锚链恢复力刚度系数。分析了对称式布置锚链系统的非线性程度 ,阐述对称式布置锚链系统所提供的恢复力作线性化处理的可行性及方法 ,并给出了对称布置锚链系统的线性刚度矩阵。为各类受对称式布置锚链系统约束的浮动结构物的动力分析提供了理论依据  相似文献