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41.
42.
采用廉价的乳糖替代IPTG诱导重组别藻蓝蛋白(rAPC)在大肠杆菌(Escherichiacoli)JM109中表达,对诱导产物表达的培养基、培养条件、诱导剂的浓度和诱导时机进行了优化,将优化条件用于5L自控发酵罐进行高密度培养。结果表明,对rAPC表达的条件进行优化后,乳糖诱导rAPC的表达率可达26.8%,与IPTG的诱导结果接近;在高密度培养中,OD600最大达21.8。研究结果可为乳糖替代IPTG大规模生产rAPC奠定基础。 相似文献
43.
针对以往噪声信号发射换能器在实际工作中,存在着诸多影响正常科研试验的问题,并根据噪声信号的技术要求,以及探测方式的技术特征,我们对噪声发射换能器进行了重新设计,从选用超磁致伸缩材料作为换能器的材料入手,结合国内外一些新的设计理念,从材料性能分析,到理论设计,最后到可靠性实施方法,都进行了周密细致的研究,并完成了设计全过程。测试结果和使用性能表明,达到了预期的目的,这也是该材料在国内首次应用于单只宽频水声换能器中,开创了稀土超磁致伸缩材料应用的又一个先列。 相似文献
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J. Perbos P. Escudier F. Parisot G. Zaouche P. Vincent Y. Menard F. Manon G. Kunstmann D. Royer L. -L. Fu 《Marine Geodesy》2003,26(3):147-157
On 7 December 2001, Jason-1 was successfully launched by a Boeing Delta II rocket from the Vandenberg Air Force Base, California. The Jason-1 satellite will maintain the high accuracy altimeter service provided since 1992 by TOPEX/Poseidon (T/P), ensuring the continuity in observing and monitoring the Ocean Dynamics (intraseasonal to interannual changes, mean sea level, tides, etc.). Despite one-fourth the mass and power, the Jason-1 system has been designed to have basically the same performance as T/P, measuring sea surface topography at a centimetric level. This new CNES/NASA mission also provides near real-time data for sea state and ocean forecast. The first two months of the Jason-1 mission have been dedicated to the assessment of the overall system. The goals of this assessment phase were:
1. To assess the behavior of the spacecraft at the platform and payload levels (Jason-1 being the first program to call on the PROTEUS versatile multimission platform for Low and Medium Earth Orbit Missions developed in partnership between Alcatel Space and CNES);
2. To verify that platform performance requirements are met with respect to Jason-1 requirements;
3. To verify that payload instruments performance requirements evaluated at instrument level are met;
4. To assess the performance of the Jason-1 Ground System.
This article will display the main outputs of the assessment of the system. It will demonstrate that all the elements of the onboard and ground systems are within the specifications. Provision of data to the Jason-1 Science Working Team started at the end of March 2002. This is the goal of a six-month phase after closure of the initial assessment phase to derive the error budget of the system in terms of altimetry user products. 相似文献
1. To assess the behavior of the spacecraft at the platform and payload levels (Jason-1 being the first program to call on the PROTEUS versatile multimission platform for Low and Medium Earth Orbit Missions developed in partnership between Alcatel Space and CNES);
2. To verify that platform performance requirements are met with respect to Jason-1 requirements;
3. To verify that payload instruments performance requirements evaluated at instrument level are met;
4. To assess the performance of the Jason-1 Ground System.
This article will display the main outputs of the assessment of the system. It will demonstrate that all the elements of the onboard and ground systems are within the specifications. Provision of data to the Jason-1 Science Working Team started at the end of March 2002. This is the goal of a six-month phase after closure of the initial assessment phase to derive the error budget of the system in terms of altimetry user products. 相似文献
48.
水动力作用下管道稳定性的试验研究 总被引:2,自引:2,他引:2
利用振荡流水槽在以下两种约束条件下,研究波浪作用下直接铺设于砂质海底的管道失稳临界条件:1)管道两端自由;2)管道可水平、垂直自由运动,但流动受到限制。试验结果表明,管重无量纳数G与管道失稳的临界Fr数之间大致呈线性关系,而当G超过一定数值时,管道是侧向稳定的。管道的约束条件、砂床特性和加载速度对管道稳定性均有影响。 相似文献
49.
Acoustic behavior of gas-bearing sediments is significantly different from that of gas-free sediments. In situ velocity profiles and acoustic signal characteristics in gas-bearing sediments of the upper several meters of the sea floor in Kiel Bay are presented in this study. Observed velocities in gas-bearing sediments are both higher and lower than those of the gas-free sediments. Small amounts of gas appear to cause signal reverberation without much attenuation. whereas large amounts of gas cause substantial attenuation. 相似文献
50.
任佐皋 《海洋学报(英文版)》1984,3(4):574-585
By means of photography the author has investigated the mechanism of wave and current motion to measure simultaneously orbital path, velocity and acceleration of water particles. This new experimental system consists of a camera, a special light source, an intermittent exposure unit and a chemical liquid tracer.On the basis of theoretical analysis of combined effects of current and wave and the results of experiments, a model of total wave and current velocity is provided to calculate wave-current forces on a pile according to Morison's Formula. The calculated results from three different sets of wave and current data have been compared with experimental data and they are found in excellent agreement. 相似文献